Simultaneous quantitation of hydroxychloroquine and its metabolites in mouse blood and tissues using LC-ESI-MS/MS: An application for pharmacokinetic studies.
Identifieur interne : 000138 ( PubMed/Corpus ); précédent : 000137; suivant : 000139Simultaneous quantitation of hydroxychloroquine and its metabolites in mouse blood and tissues using LC-ESI-MS/MS: An application for pharmacokinetic studies.
Auteurs : Yashpal S. Chhonker ; Richard L. Sleightholm ; Jing Li ; David Oupick ; Daryl J. MurrySource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1873-376X ] ; 2018.
English descriptors
- KwdEn :
- Animals, Chromatography, Liquid (methods), Hydroxychloroquine (analysis), Hydroxychloroquine (blood), Hydroxychloroquine (chemistry), Hydroxychloroquine (pharmacokinetics), Limit of Detection, Linear Models, Liver (chemistry), Liver (metabolism), Lung (chemistry), Lung (metabolism), Male, Mice, Mice, Inbred BALB C, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization (methods), Tandem Mass Spectrometry (methods), Tissue Distribution.
- MESH :
- chemical , analysis : Hydroxychloroquine.
- chemical , blood : Hydroxychloroquine.
- chemical , chemistry : Hydroxychloroquine.
- chemistry : Liver, Lung.
- metabolism : Liver, Lung.
- methods : Chromatography, Liquid, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry.
- chemical , pharmacokinetics : Hydroxychloroquine.
- Animals, Limit of Detection, Linear Models, Male, Mice, Mice, Inbred BALB C, Reproducibility of Results, Tissue Distribution.
Abstract
Hydroxychloroquine (HCQ) has been shown to disrupt autophagy and sensitize cancer cells to radiation and chemotherapeutic agents. However, the optimal delivery method, dose, and tumor concentrations required for these effects are not known. This is in part due to a lack of sensitive and reproducible analytical methods for HCQ quantitation in small animals. As such, we developed and validated a selective and sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for simultaneous quantitation of hydroxychloroquine and its metabolites in mouse blood and tissues. The chromatographic separation and detection of analytes were achieved on a reversed phase Thermo Aquasil C18 (50×4.6mm, 3μ) column, with gradient elution using 0.2% formic acid and 0.1% formic acid in methanol as mobile phase at a flow rate of 0.5mL/min. Simple protein precipitation was utilized for extraction of analytes from the desired matrix. Analytes were separated and quantitated using MS/MS with an electrospray ionization source in positive multiple reaction monitoring (MRM) mode. The MS/MS response was linear over the concentration range from 1 to 2000ng/mL for all analytes with a correlation coefficient (R2) of 0.998 or better. The within- and between-day precision (relative standard deviation, % RSD) and accuracy were within the acceptable limits per FDA guidelines. The validated method was successfully applied to a preclinical pharmacokinetic mouse study involving low volume blood and tissue samples for hydroxychloroquine and metabolites.
DOI: 10.1016/j.jchromb.2017.11.026
PubMed: 29207305
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Sleightholm</name><affiliation><nlm:affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name><affiliation><nlm:affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Oupick, David" sort="Oupick, David" uniqKey="Oupick D" first="David" last="Oupick">David Oupick</name><affiliation><nlm:affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States; Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Murry, Daryl J" sort="Murry, Daryl J" uniqKey="Murry D" first="Daryl J" last="Murry">Daryl J. Murry</name><affiliation><nlm:affiliation>Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, United States; Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, United States. 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Chhonker</name><affiliation><nlm:affiliation>Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Sleightholm, Richard L" sort="Sleightholm, Richard L" uniqKey="Sleightholm R" first="Richard L" last="Sleightholm">Richard L. Sleightholm</name><affiliation><nlm:affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name><affiliation><nlm:affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Oupick, David" sort="Oupick, David" uniqKey="Oupick D" first="David" last="Oupick">David Oupick</name><affiliation><nlm:affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States; Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Murry, Daryl J" sort="Murry, Daryl J" uniqKey="Murry D" first="Daryl J" last="Murry">Daryl J. Murry</name><affiliation><nlm:affiliation>Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, United States; Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, United States. Electronic address: dj.murry@unmc.edu.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><idno type="eISSN">1873-376X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chromatography, Liquid (methods)</term><term>Hydroxychloroquine (analysis)</term><term>Hydroxychloroquine (blood)</term><term>Hydroxychloroquine (chemistry)</term><term>Hydroxychloroquine (pharmacokinetics)</term><term>Limit of Detection</term><term>Linear Models</term><term>Liver (chemistry)</term><term>Liver (metabolism)</term><term>Lung (chemistry)</term><term>Lung (metabolism)</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Reproducibility of Results</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term><term>Tandem Mass Spectrometry (methods)</term><term>Tissue Distribution</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hydroxychloroquine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Hydroxychloroquine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hydroxychloroquine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Liver</term><term>Lung</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Liver</term><term>Lung</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Liquid</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Hydroxychloroquine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Limit of Detection</term><term>Linear Models</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Reproducibility of Results</term><term>Tissue Distribution</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hydroxychloroquine (HCQ) has been shown to disrupt autophagy and sensitize cancer cells to radiation and chemotherapeutic agents. However, the optimal delivery method, dose, and tumor concentrations required for these effects are not known. This is in part due to a lack of sensitive and reproducible analytical methods for HCQ quantitation in small animals. As such, we developed and validated a selective and sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for simultaneous quantitation of hydroxychloroquine and its metabolites in mouse blood and tissues. The chromatographic separation and detection of analytes were achieved on a reversed phase Thermo Aquasil C<sub>18</sub> (50×4.6mm, 3μ) column, with gradient elution using 0.2% formic acid and 0.1% formic acid in methanol as mobile phase at a flow rate of 0.5mL/min. Simple protein precipitation was utilized for extraction of analytes from the desired matrix. Analytes were separated and quantitated using MS/MS with an electrospray ionization source in positive multiple reaction monitoring (MRM) mode. The MS/MS response was linear over the concentration range from 1 to 2000ng/mL for all analytes with a correlation coefficient (R<sup>2</sup>) of 0.998 or better. The within- and between-day precision (relative standard deviation, % RSD) and accuracy were within the acceptable limits per FDA guidelines. The validated method was successfully applied to a preclinical pharmacokinetic mouse study involving low volume blood and tissue samples for hydroxychloroquine and metabolites.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29207305</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-376X</ISSN><JournalIssue CitedMedium="Internet"><Volume>1072</Volume><PubDate><Year>2018</Year><Month>Jan</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</Title><ISOAbbreviation>J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.</ISOAbbreviation></Journal><ArticleTitle>Simultaneous quantitation of hydroxychloroquine and its metabolites in mouse blood and tissues using LC-ESI-MS/MS: An application for pharmacokinetic studies.</ArticleTitle><Pagination><MedlinePgn>320-327</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1570-0232(17)31382-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jchromb.2017.11.026</ELocationID><Abstract><AbstractText>Hydroxychloroquine (HCQ) has been shown to disrupt autophagy and sensitize cancer cells to radiation and chemotherapeutic agents. However, the optimal delivery method, dose, and tumor concentrations required for these effects are not known. This is in part due to a lack of sensitive and reproducible analytical methods for HCQ quantitation in small animals. As such, we developed and validated a selective and sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for simultaneous quantitation of hydroxychloroquine and its metabolites in mouse blood and tissues. The chromatographic separation and detection of analytes were achieved on a reversed phase Thermo Aquasil C<sub>18</sub> (50×4.6mm, 3μ) column, with gradient elution using 0.2% formic acid and 0.1% formic acid in methanol as mobile phase at a flow rate of 0.5mL/min. Simple protein precipitation was utilized for extraction of analytes from the desired matrix. Analytes were separated and quantitated using MS/MS with an electrospray ionization source in positive multiple reaction monitoring (MRM) mode. The MS/MS response was linear over the concentration range from 1 to 2000ng/mL for all analytes with a correlation coefficient (R<sup>2</sup>) of 0.998 or better. The within- and between-day precision (relative standard deviation, % RSD) and accuracy were within the acceptable limits per FDA guidelines. The validated method was successfully applied to a preclinical pharmacokinetic mouse study involving low volume blood and tissue samples for hydroxychloroquine and metabolites.</AbstractText><CopyrightInformation>Copyright © 2017 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chhonker</LastName><ForeName>Yashpal S</ForeName><Initials>YS</Initials><AffiliationInfo><Affiliation>Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sleightholm</LastName><ForeName>Richard L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oupický</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, United States; Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murry</LastName><ForeName>Daryl J</ForeName><Initials>DJ</Initials><AffiliationInfo><Affiliation>Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, United States; Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, United States. Electronic address: dj.murry@unmc.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P30 CA036727</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>11</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Chromatogr B Analyt Technol Biomed Life Sci</MedlineTA><NlmUniqueID>101139554</NlmUniqueID><ISSNLinking>1570-0232</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>4QWG6N8QKH</RegistryNumber><NameOfSubstance UI="D006886">Hydroxychloroquine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" 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IdType="pubmed">26458814</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pharm Biomed Anal. 2014 Nov;100:131-137</Citation><ArticleIdList><ArticleId IdType="pubmed">25165008</ArticleId></ArticleIdList></Reference><Reference><Citation>Future Sci OA. 2015 Nov 01;1(3):FSO26</Citation><ArticleIdList><ArticleId IdType="pubmed">28031899</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Antimicrob Agents. 2007 Oct;30(4):297-308</Citation><ArticleIdList><ArticleId IdType="pubmed">17629679</ArticleId></ArticleIdList></Reference><Reference><Citation>Rheumatol Ther. 2015 Dec;2(2):183-195</Citation><ArticleIdList><ArticleId IdType="pubmed">27747530</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chromatogr. 1988 Dec 9;433:197-206</Citation><ArticleIdList><ArticleId IdType="pubmed">3235547</ArticleId></ArticleIdList></Reference><Reference><Citation>Forensic Sci Int. 2002 Mar 28;126(1):17-23</Citation><ArticleIdList><ArticleId IdType="pubmed">11955826</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Sep 30;8(9):e74216</Citation><ArticleIdList><ArticleId IdType="pubmed">24098639</ArticleId></ArticleIdList></Reference><Reference><Citation>Mass Spectrom Rev. 2003 May-Jun;22(3):195-214</Citation><ArticleIdList><ArticleId IdType="pubmed">12838545</ArticleId></ArticleIdList></Reference><Reference><Citation>Antimicrob Agents Chemother. 2009 Apr;53(4):1468-75</Citation><ArticleIdList><ArticleId IdType="pubmed">19188392</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 2011 Apr 1;25(7):717-29</Citation><ArticleIdList><ArticleId IdType="pubmed">21406549</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Rheum Dis. 2013 Nov;72(11):1786-92</Citation><ArticleIdList><ArticleId IdType="pubmed">23144449</ArticleId></ArticleIdList></Reference><Reference><Citation>Arthritis Rheum. 2002 Jun;46(6):1460-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12115175</ArticleId></ArticleIdList></Reference><Reference><Citation>J Thorac Oncol. 2012 Oct;7(10):1602-8</Citation><ArticleIdList><ArticleId IdType="pubmed">22878749</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioanalysis. 2012 Oct;4(19):2391-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23088465</ArticleId></ArticleIdList></Reference><Reference><Citation>Arthritis Rheum. 2006 Oct;54(10):3284-90</Citation><ArticleIdList><ArticleId IdType="pubmed">17009263</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Pharmacol. 1994 Nov;34(11):1088-97</Citation><ArticleIdList><ArticleId IdType="pubmed">7876401</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chromatogr. 1985 Nov 8;344:241-8</Citation><ArticleIdList><ArticleId IdType="pubmed">4086544</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pharm Biomed Anal. 2012 Mar 5;61:86-92</Citation><ArticleIdList><ArticleId IdType="pubmed">22197155</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Chim Acta. 2013 Jun 5;421:79-84</Citation><ArticleIdList><ArticleId IdType="pubmed">23485647</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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