Degradation of group V secretory phospholipase A2 in lung endothelium is mediated by autophagy.
Identifieur interne : 000023 ( PubMed/Corpus ); précédent : 000022; suivant : 000024Degradation of group V secretory phospholipase A2 in lung endothelium is mediated by autophagy.
Auteurs : Lucille N. Meliton ; Xiangdong Zhu ; Mary Brown ; Yulia Epshtein ; Takeshi Kawasaki ; Eleftheria Letsiou ; Steven M. DudekSource :
- Microvascular research [ 1095-9319 ] ; 2020.
Abstract
Group V secretory phospholipase A2 (gVPLA2) is a potent inflammatory mediator in mammalian tissues that hydrolyzes phospholipids and initiates eicosanoid biosynthesis. Previous work has demonstrated that multiple inflammatory stimuli induce its expression and secretion in several cell types, including the lung endothelium. However, little is known about the mechanism(s) by which gVPLA2 inflammatory signaling is subsequently downregulated. Therefore, in this study we characterized potential clearance mechanisms for gVPLA2 in lung endothelial cells (EC). We observed that exogenous gVPLA2 is taken up rapidly by nutrient-starved human pulmonary artery EC (HPAEC) in vitro, and its cellular expression subsequently is reduced over several hours. In parallel experiments performed in pulmonary vascular EC isolated from mice genetically deficient in gVPLA2, the degradation of exogenously applied gVPLA2 occurs in a qualitatively similar fashion. This degradation is significantly attenuated in EC treated with ammonium chloride or chloroquine, which are lysosomal inhibitors that block autophagic flux. In contrast, the proteasomal inhibitor MG132 fails to prevent the clearance of gVPLA2. Both immunofluorescence microscopy and proximity ligation assay demonstrate the co-localization of LC3 and gVPLA2 during this process, indicating the association of gVPLA2 with autophagosomes. Nutrient starvation, a known inducer of autophagy, is sufficient to stimulate gVPLA2 degradation. These results suggest that a lysosome-mediated autophagy pathway contributes to gVPLA2 clearance from lung EC. These novel observations advance our understanding of the mechanism by which this key inflammatory enzyme is downregulated in the lung vasculature.
DOI: 10.1016/j.mvr.2019.103954
PubMed: 31730773
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pubmed:31730773Le document en format XML
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in lung endothelium is mediated by autophagy.</title>
<author><name sortKey="Meliton, Lucille N" sort="Meliton, Lucille N" uniqKey="Meliton L" first="Lucille N" last="Meliton">Lucille N. Meliton</name>
<affiliation><nlm:affiliation>Division of Pulmonary, Critical Care, Sleep and Allergy, University of Illinois Department of Medicine, Chicago, IL, United States of America.</nlm:affiliation>
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<author><name sortKey="Zhu, Xiangdong" sort="Zhu, Xiangdong" uniqKey="Zhu X" first="Xiangdong" last="Zhu">Xiangdong Zhu</name>
<affiliation><nlm:affiliation>Department of Emergency Medicine, University of Illinois College of Medicine, Chicago, IL, United States of America.</nlm:affiliation>
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<author><name sortKey="Brown, Mary" sort="Brown, Mary" uniqKey="Brown M" first="Mary" last="Brown">Mary Brown</name>
<affiliation><nlm:affiliation>The Indiana Center for Biological Microscopy, Indiana University School of Medicine, United States of America.</nlm:affiliation>
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<author><name sortKey="Epshtein, Yulia" sort="Epshtein, Yulia" uniqKey="Epshtein Y" first="Yulia" last="Epshtein">Yulia Epshtein</name>
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<author><name sortKey="Kawasaki, Takeshi" sort="Kawasaki, Takeshi" uniqKey="Kawasaki T" first="Takeshi" last="Kawasaki">Takeshi Kawasaki</name>
<affiliation><nlm:affiliation>Department of Respirology, Chiba University, Chiba, Japan.</nlm:affiliation>
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<author><name sortKey="Letsiou, Eleftheria" sort="Letsiou, Eleftheria" uniqKey="Letsiou E" first="Eleftheria" last="Letsiou">Eleftheria Letsiou</name>
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<author><name sortKey="Dudek, Steven M" sort="Dudek, Steven M" uniqKey="Dudek S" first="Steven M" last="Dudek">Steven M. Dudek</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Degradation of group V secretory phospholipase A<sub>2</sub>
in lung endothelium is mediated by autophagy.</title>
<author><name sortKey="Meliton, Lucille N" sort="Meliton, Lucille N" uniqKey="Meliton L" first="Lucille N" last="Meliton">Lucille N. Meliton</name>
<affiliation><nlm:affiliation>Division of Pulmonary, Critical Care, Sleep and Allergy, University of Illinois Department of Medicine, Chicago, IL, United States of America.</nlm:affiliation>
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<author><name sortKey="Zhu, Xiangdong" sort="Zhu, Xiangdong" uniqKey="Zhu X" first="Xiangdong" last="Zhu">Xiangdong Zhu</name>
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<author><name sortKey="Brown, Mary" sort="Brown, Mary" uniqKey="Brown M" first="Mary" last="Brown">Mary Brown</name>
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<author><name sortKey="Epshtein, Yulia" sort="Epshtein, Yulia" uniqKey="Epshtein Y" first="Yulia" last="Epshtein">Yulia Epshtein</name>
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<author><name sortKey="Letsiou, Eleftheria" sort="Letsiou, Eleftheria" uniqKey="Letsiou E" first="Eleftheria" last="Letsiou">Eleftheria Letsiou</name>
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<series><title level="j">Microvascular research</title>
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<front><div type="abstract" xml:lang="en">Group V secretory phospholipase A<sub>2</sub>
(gVPLA<sub>2</sub>
) is a potent inflammatory mediator in mammalian tissues that hydrolyzes phospholipids and initiates eicosanoid biosynthesis. Previous work has demonstrated that multiple inflammatory stimuli induce its expression and secretion in several cell types, including the lung endothelium. However, little is known about the mechanism(s) by which gVPLA<sub>2</sub>
inflammatory signaling is subsequently downregulated. Therefore, in this study we characterized potential clearance mechanisms for gVPLA<sub>2</sub>
in lung endothelial cells (EC). We observed that exogenous gVPLA<sub>2</sub>
is taken up rapidly by nutrient-starved human pulmonary artery EC (HPAEC) in vitro, and its cellular expression subsequently is reduced over several hours. In parallel experiments performed in pulmonary vascular EC isolated from mice genetically deficient in gVPLA<sub>2</sub>
, the degradation of exogenously applied gVPLA<sub>2</sub>
occurs in a qualitatively similar fashion. This degradation is significantly attenuated in EC treated with ammonium chloride or chloroquine, which are lysosomal inhibitors that block autophagic flux. In contrast, the proteasomal inhibitor MG132 fails to prevent the clearance of gVPLA<sub>2</sub>
. Both immunofluorescence microscopy and proximity ligation assay demonstrate the co-localization of LC3 and gVPLA<sub>2</sub>
during this process, indicating the association of gVPLA<sub>2</sub>
with autophagosomes. Nutrient starvation, a known inducer of autophagy, is sufficient to stimulate gVPLA<sub>2</sub>
degradation. These results suggest that a lysosome-mediated autophagy pathway contributes to gVPLA<sub>2</sub>
clearance from lung EC. These novel observations advance our understanding of the mechanism by which this key inflammatory enzyme is downregulated in the lung vasculature.</div>
</front>
</TEI>
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<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>20</Day>
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<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-9319</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>129</Volume>
<PubDate><Year>2020</Year>
<Month>May</Month>
</PubDate>
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<Title>Microvascular research</Title>
<ISOAbbreviation>Microvasc. Res.</ISOAbbreviation>
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<ArticleTitle>Degradation of group V secretory phospholipase A<sub>2</sub>
in lung endothelium is mediated by autophagy.</ArticleTitle>
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<Abstract><AbstractText>Group V secretory phospholipase A<sub>2</sub>
(gVPLA<sub>2</sub>
) is a potent inflammatory mediator in mammalian tissues that hydrolyzes phospholipids and initiates eicosanoid biosynthesis. Previous work has demonstrated that multiple inflammatory stimuli induce its expression and secretion in several cell types, including the lung endothelium. However, little is known about the mechanism(s) by which gVPLA<sub>2</sub>
inflammatory signaling is subsequently downregulated. Therefore, in this study we characterized potential clearance mechanisms for gVPLA<sub>2</sub>
in lung endothelial cells (EC). We observed that exogenous gVPLA<sub>2</sub>
is taken up rapidly by nutrient-starved human pulmonary artery EC (HPAEC) in vitro, and its cellular expression subsequently is reduced over several hours. In parallel experiments performed in pulmonary vascular EC isolated from mice genetically deficient in gVPLA<sub>2</sub>
, the degradation of exogenously applied gVPLA<sub>2</sub>
occurs in a qualitatively similar fashion. This degradation is significantly attenuated in EC treated with ammonium chloride or chloroquine, which are lysosomal inhibitors that block autophagic flux. In contrast, the proteasomal inhibitor MG132 fails to prevent the clearance of gVPLA<sub>2</sub>
. Both immunofluorescence microscopy and proximity ligation assay demonstrate the co-localization of LC3 and gVPLA<sub>2</sub>
during this process, indicating the association of gVPLA<sub>2</sub>
with autophagosomes. Nutrient starvation, a known inducer of autophagy, is sufficient to stimulate gVPLA<sub>2</sub>
degradation. These results suggest that a lysosome-mediated autophagy pathway contributes to gVPLA<sub>2</sub>
clearance from lung EC. These novel observations advance our understanding of the mechanism by which this key inflammatory enzyme is downregulated in the lung vasculature.</AbstractText>
<CopyrightInformation>Copyright © 2019 Elsevier Inc. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meliton</LastName>
<ForeName>Lucille N</ForeName>
<Initials>LN</Initials>
<AffiliationInfo><Affiliation>Division of Pulmonary, Critical Care, Sleep and Allergy, University of Illinois Department of Medicine, Chicago, IL, United States of America.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Xiangdong</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Department of Emergency Medicine, University of Illinois College of Medicine, Chicago, IL, United States of America.</Affiliation>
</AffiliationInfo>
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<AffiliationInfo><Affiliation>The Indiana Center for Biological Microscopy, Indiana University School of Medicine, United States of America.</Affiliation>
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<AffiliationInfo><Affiliation>Division of Pulmonary, Critical Care, Sleep and Allergy, University of Illinois Department of Medicine, Chicago, IL, United States of America.</Affiliation>
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<Author ValidYN="Y"><LastName>Kawasaki</LastName>
<ForeName>Takeshi</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Respirology, Chiba University, Chiba, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Letsiou</LastName>
<ForeName>Eleftheria</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Division of Pulmonary, Critical Care, Sleep and Allergy, University of Illinois Department of Medicine, Chicago, IL, United States of America.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Dudek</LastName>
<ForeName>Steven M</ForeName>
<Initials>SM</Initials>
<AffiliationInfo><Affiliation>Division of Pulmonary, Critical Care, Sleep and Allergy, University of Illinois Department of Medicine, Chicago, IL, United States of America. Electronic address: sdudek@uic.edu.</Affiliation>
</AffiliationInfo>
</Author>
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<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>11</Month>
<Day>13</Day>
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<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Microvasc Res</MedlineTA>
<NlmUniqueID>0165035</NlmUniqueID>
<ISSNLinking>0026-2862</ISSNLinking>
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<CitationSubset>IM</CitationSubset>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Autophagy</Keyword>
<Keyword MajorTopicYN="N">Endothelium</Keyword>
<Keyword MajorTopicYN="N">Group V secretory phospholipase A(2)</Keyword>
<Keyword MajorTopicYN="N">Lysosomes</Keyword>
<Keyword MajorTopicYN="N">Protein degradation</Keyword>
</KeywordList>
<CoiStatement>Declaration of competing interest None.</CoiStatement>
</MedlineCitation>
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<Month>02</Month>
<Day>28</Day>
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<PubMedPubDate PubStatus="revised"><Year>2019</Year>
<Month>09</Month>
<Day>18</Day>
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