Blocking transmembrane219 protein signaling inhibits autophagy and restores normal cell death
Identifieur interne : 000772 ( Pmc/Curation ); précédent : 000771; suivant : 000773Blocking transmembrane219 protein signaling inhibits autophagy and restores normal cell death
Auteurs : Sean Joyce ; Adel M. NourSource :
- PLoS ONE [ 1932-6203 ] ; 2019.
Abstract
Autophagy plays a vital role in tumor therapy and survival of dormant tumor cells. Here we describe a novel function of a protein known as Transmembrane 219 (TM219) as an autophagy activator. TM219 is a small membrane protein expressed in all known human tissues except the thymus. We used biochemical approaches to identify calmodulin and calmodulin dependent protein kinase II as a part of TM219 protein complex. Then, we employed
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DOI: 10.1371/journal.pone.0218091
PubMed: 31220095
PubMed Central: 6586287
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Blocking transmembrane219 protein signaling inhibits autophagy and restores normal cell death</title><author><name sortKey="Joyce, Sean" sort="Joyce, Sean" uniqKey="Joyce S" first="Sean" last="Joyce">Sean Joyce</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author><author><name sortKey="Nour, Adel M" sort="Nour, Adel M" uniqKey="Nour A" first="Adel M." last="Nour">Adel M. Nour</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31220095</idno><idno type="pmc">6586287</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586287</idno><idno type="RBID">PMC:6586287</idno><idno type="doi">10.1371/journal.pone.0218091</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000772</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000772</idno><idno type="wicri:Area/Pmc/Curation">000772</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000772</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Blocking transmembrane219 protein signaling inhibits autophagy and restores normal cell death</title><author><name sortKey="Joyce, Sean" sort="Joyce, Sean" uniqKey="Joyce S" first="Sean" last="Joyce">Sean Joyce</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author><author><name sortKey="Nour, Adel M" sort="Nour, Adel M" uniqKey="Nour A" first="Adel M." last="Nour">Adel M. Nour</name><affiliation><nlm:aff id="aff001"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Autophagy plays a vital role in tumor therapy and survival of dormant tumor cells. Here we describe a novel function of a protein known as Transmembrane 219 (TM219) as an autophagy activator. TM219 is a small membrane protein expressed in all known human tissues except the thymus. We used biochemical approaches to identify calmodulin and calmodulin dependent protein kinase II as a part of TM219 protein complex. Then, we employed <italic>in vitro</italic> reconstitution system and fluorescence anisotropy to study the requirements of TM219 to bind calmodulin <italic>in vitro</italic>. We also used this system to study the effects of a synthetic peptide derived from the sequence of the short cytoplasmic tail of TM219 (SCTT) on calmodulin-TM219 receptor interactions. We conjugated SCTT peptide with a pH Low Insertion peptide (pHLIP) for optimal cellular delivery. We finally tested the effects of SCTT-pHLIP on triple negative human breast cancer cells in three dimension culture. Our data defined a novel function of TM219 protein and an efficient approach to inhibit it.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chavez, K J" uniqKey="Chavez K">K. J. Chavez</name></author><author><name sortKey="Garimella, S V" uniqKey="Garimella S">S. V. Garimella</name></author><author><name sortKey="Lipkowitz, S" uniqKey="Lipkowitz S">S. Lipkowitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z. Wang</name></author><author><name sortKey="Shi, X" uniqKey="Shi X">X. Shi</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Fan, J" uniqKey="Fan J">J. Fan</name></author><author><name sortKey="Zeng, X" uniqKey="Zeng X">X. Zeng</name></author><author><name sortKey="Xian, Z" uniqKey="Xian Z">Z. Xian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author><author><name sortKey="Dupont, N" uniqKey="Dupont N">N. Dupont</name></author><author><name sortKey="Castillo, E F" uniqKey="Castillo E">E. F. Castillo</name></author><author><name sortKey="Deretic, V" uniqKey="Deretic V">V. Deretic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eskelinen, E L" uniqKey="Eskelinen E">E. L. Eskelinen</name></author><author><name sortKey="Saftig, P" uniqKey="Saftig P">P. Saftig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, J Y" uniqKey="Guo J">J. Y. Guo</name></author><author><name sortKey="Karsli Uzunbas, G" uniqKey="Karsli Uzunbas G">G. Karsli-Uzunbas</name></author><author><name sortKey="Mathew, R" uniqKey="Mathew R">R. Mathew</name></author><author><name sortKey="Aisner, S C" uniqKey="Aisner S">S. C. Aisner</name></author><author><name sortKey="Kamphorst, J J" uniqKey="Kamphorst J">J. J. Kamphorst</name></author><author><name sortKey="Strohecker, A M" uniqKey="Strohecker A">A. M. Strohecker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, X" uniqKey="Xie X">X. Xie</name></author><author><name sortKey="Koh, J Y" uniqKey="Koh J">J. Y. Koh</name></author><author><name sortKey="Price, S" uniqKey="Price S">S. Price</name></author><author><name sortKey="White, E" uniqKey="White E">E. White</name></author><author><name sortKey="Mehnert, J M" uniqKey="Mehnert J">J. M. Mehnert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karsli Uzunbas, G" uniqKey="Karsli Uzunbas G">G. Karsli-Uzunbas</name></author><author><name sortKey="Guo, J Y" uniqKey="Guo J">J. Y. Guo</name></author><author><name sortKey="Price, S" uniqKey="Price S">S. Price</name></author><author><name sortKey="Teng, X" uniqKey="Teng X">X. Teng</name></author><author><name sortKey="Laddha, S V" uniqKey="Laddha S">S. V. Laddha</name></author><author><name sortKey="Khor, S" uniqKey="Khor S">S. Khor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wirawan, E" uniqKey="Wirawan E">E. Wirawan</name></author><author><name sortKey="Lippens, S" uniqKey="Lippens S">S. Lippens</name></author><author><name sortKey="Vanden Berghe, T" uniqKey="Vanden Berghe T">T. Vanden Berghe</name></author><author><name sortKey="Romagnoli, A" uniqKey="Romagnoli A">A. Romagnoli</name></author><author><name sortKey="Fimia, G M" uniqKey="Fimia G">G. M. Fimia</name></author><author><name sortKey="Piacentini, M" uniqKey="Piacentini M">M. Piacentini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Russell, R C" uniqKey="Russell R">R. C. Russell</name></author><author><name sortKey="Tian, Y" uniqKey="Tian Y">Y. Tian</name></author><author><name sortKey="Yuan, H" uniqKey="Yuan H">H. Yuan</name></author><author><name sortKey="Park, H W" uniqKey="Park H">H. W. Park</name></author><author><name sortKey="Chang, Y Y" uniqKey="Chang Y">Y. Y. Chang</name></author><author><name sortKey="Kim, J" uniqKey="Kim J">J. Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levin Salomon, V" uniqKey="Levin Salomon V">V. Levin-Salomon</name></author><author><name sortKey="Bialik, S" uniqKey="Bialik S">S. Bialik</name></author><author><name sortKey="Kimchi, A" uniqKey="Kimchi A">A. Kimchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qian, X" uniqKey="Qian X">X. Qian</name></author><author><name sortKey="Li, X" uniqKey="Li X">X. Li</name></author><author><name sortKey="Cai, Q" uniqKey="Cai Q">Q. Cai</name></author><author><name sortKey="Zhang, C" uniqKey="Zhang C">C. Zhang</name></author><author><name sortKey="Yu, Q" uniqKey="Yu Q">Q. Yu</name></author><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y. Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shimizu, S" uniqKey="Shimizu S">S. Shimizu</name></author><author><name sortKey="Kanaseki, T" uniqKey="Kanaseki T">T. Kanaseki</name></author><author><name sortKey="Mizushima, N" uniqKey="Mizushima N">N. Mizushima</name></author><author><name sortKey="Mizuta, T" uniqKey="Mizuta T">T. Mizuta</name></author><author><name sortKey="Arakawa Kobayashi, S" uniqKey="Arakawa Kobayashi S">S. Arakawa-Kobayashi</name></author><author><name sortKey="Thompson, C B" uniqKey="Thompson C">C. B. Thompson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yue, Z" uniqKey="Yue Z">Z. Yue</name></author><author><name sortKey="Zhong, Y" uniqKey="Zhong Y">Y. Zhong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, Z" uniqKey="Lu Z">Z. Lu</name></author><author><name sortKey="Luo, R Z" uniqKey="Luo R">R. Z. Luo</name></author><author><name sortKey="Lu, Y" uniqKey="Lu Y">Y. Lu</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Yu, Q" uniqKey="Yu Q">Q. Yu</name></author><author><name sortKey="Khare, S" uniqKey="Khare S">S. Khare</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sosa, M S" uniqKey="Sosa M">M. S. Sosa</name></author><author><name sortKey="Bragado, P" uniqKey="Bragado P">P. Bragado</name></author><author><name sortKey="Aguirre Ghiso, J A" uniqKey="Aguirre Ghiso J">J. A. Aguirre-Ghiso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giancotti, F G" uniqKey="Giancotti F">F. G. Giancotti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolf, J" uniqKey="Wolf J">J. Wolf</name></author><author><name sortKey="Dewi, D L" uniqKey="Dewi D">D. L. Dewi</name></author><author><name sortKey="Fredebohm, J" uniqKey="Fredebohm J">J. Fredebohm</name></author><author><name sortKey="Muller Decker, K" uniqKey="Muller Decker K">K. Muller-Decker</name></author><author><name sortKey="Flechtenmacher, C" uniqKey="Flechtenmacher C">C. Flechtenmacher</name></author><author><name sortKey="Hoheisel, J D" uniqKey="Hoheisel J">J. D. Hoheisel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cufi, S" uniqKey="Cufi S">S. Cufi</name></author><author><name sortKey="Vazquez Martin, A" uniqKey="Vazquez Martin A">A. Vazquez-Martin</name></author><author><name sortKey="Oliveras Ferraros, C" uniqKey="Oliveras Ferraros C">C. Oliveras-Ferraros</name></author><author><name sortKey="Martin Castillo, B" uniqKey="Martin Castillo B">B. Martin-Castillo</name></author><author><name sortKey="Vellon, L" uniqKey="Vellon L">L. Vellon</name></author><author><name sortKey="Menendez, J A" uniqKey="Menendez J">J. A. Menendez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mizushima, N" uniqKey="Mizushima N">N. Mizushima</name></author><author><name sortKey="Noda, T" uniqKey="Noda T">T. Noda</name></author><author><name sortKey="Yoshimori, T" uniqKey="Yoshimori T">T. Yoshimori</name></author><author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y. Tanaka</name></author><author><name sortKey="Ishii, T" uniqKey="Ishii T">T. Ishii</name></author><author><name sortKey="George, M D" uniqKey="George M">M. D. George</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thumm, M" uniqKey="Thumm M">M. Thumm</name></author><author><name sortKey="Egner, R" uniqKey="Egner R">R. Egner</name></author><author><name sortKey="Koch, B" uniqKey="Koch B">B. Koch</name></author><author><name sortKey="Schlumpberger, M" uniqKey="Schlumpberger M">M. Schlumpberger</name></author><author><name sortKey="Straub, M" uniqKey="Straub M">M. Straub</name></author><author><name sortKey="Veenhuis, M" uniqKey="Veenhuis M">M. Veenhuis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Janji, B" uniqKey="Janji B">B. Janji</name></author><author><name sortKey="Viry, E" uniqKey="Viry E">E. Viry</name></author><author><name sortKey="Moussay, E" uniqKey="Moussay E">E. Moussay</name></author><author><name sortKey="Paggetti, J" uniqKey="Paggetti J">J. Paggetti</name></author><author><name sortKey="Arakelian, T" uniqKey="Arakelian T">T. Arakelian</name></author><author><name sortKey="Mgrditchian, T" uniqKey="Mgrditchian T">T. Mgrditchian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kumar, P" uniqKey="Kumar P">P. Kumar</name></author><author><name sortKey="Zhang, D M" uniqKey="Zhang D">D. M. Zhang</name></author><author><name sortKey="Degenhardt, K" uniqKey="Degenhardt K">K. Degenhardt</name></author><author><name sortKey="Chen, Z S" uniqKey="Chen Z">Z. S. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiang, L" uniqKey="Qiang L">L. Qiang</name></author><author><name sortKey="Zhao, B" uniqKey="Zhao B">B. Zhao</name></author><author><name sortKey="Shah, P" uniqKey="Shah P">P. Shah</name></author><author><name sortKey="Sample, A" uniqKey="Sample A">A. Sample</name></author><author><name sortKey="Yang, S" uniqKey="Yang S">S. Yang</name></author><author><name sortKey="He, Y Y" uniqKey="He Y">Y. Y. He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gomis, R R" uniqKey="Gomis R">R. R. Gomis</name></author><author><name sortKey="Gawrzak, S" uniqKey="Gawrzak S">S. Gawrzak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goehe, R W" uniqKey="Goehe R">R. W. Goehe</name></author><author><name sortKey="Di, X" uniqKey="Di X">X. Di</name></author><author><name sortKey="Sharma, K" uniqKey="Sharma K">K. Sharma</name></author><author><name sortKey="Bristol, M L" uniqKey="Bristol M">M. L. Bristol</name></author><author><name sortKey="Henderson, S C" uniqKey="Henderson S">S. C. Henderson</name></author><author><name sortKey="Valerie, K" uniqKey="Valerie K">K. Valerie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Forbes, B E" uniqKey="Forbes B">B. E. Forbes</name></author><author><name sortKey="Mccarthy, P" uniqKey="Mccarthy P">P. McCarthy</name></author><author><name sortKey="Norton, R S" uniqKey="Norton R">R. S. Norton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baxter, R C" uniqKey="Baxter R">R. C. Baxter</name></author><author><name sortKey="Dai, J" uniqKey="Dai J">J. Dai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weinzimer, S A" uniqKey="Weinzimer S">S. A. Weinzimer</name></author><author><name sortKey="Gibson, T B" uniqKey="Gibson T">T. B. Gibson</name></author><author><name sortKey="Collett Solberg, P F" uniqKey="Collett Solberg P">P. F. Collett-Solberg</name></author><author><name sortKey="Khare, A" uniqKey="Khare A">A. Khare</name></author><author><name sortKey="Liu, B" uniqKey="Liu B">B. Liu</name></author><author><name sortKey="Cohen, P" uniqKey="Cohen P">P. Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gui, Y" uniqKey="Gui Y">Y. Gui</name></author><author><name sortKey="Murphy, L J" uniqKey="Murphy L">L. J. Murphy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Campbell, P G" uniqKey="Campbell P">P. G. Campbell</name></author><author><name sortKey="Durham, S K" uniqKey="Durham S">S. K. Durham</name></author><author><name sortKey="Suwanichkul, A" uniqKey="Suwanichkul A">A. Suwanichkul</name></author><author><name sortKey="Hayes, J D" uniqKey="Hayes J">J. D. Hayes</name></author><author><name sortKey="Powell, D R" uniqKey="Powell D">D. R. Powell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, S S" uniqKey="Huang S">S. S. Huang</name></author><author><name sortKey="Ling, T Y" uniqKey="Ling T">T. Y. Ling</name></author><author><name sortKey="Tseng, W F" uniqKey="Tseng W">W. F. Tseng</name></author><author><name sortKey="Huang, Y H" uniqKey="Huang Y">Y. H. Huang</name></author><author><name sortKey="Tang, F M" uniqKey="Tang F">F. M. Tang</name></author><author><name sortKey="Leal, S M" uniqKey="Leal S">S. M. Leal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ingermann, A R" uniqKey="Ingermann A">A. R. Ingermann</name></author><author><name sortKey="Yang, Y F" uniqKey="Yang Y">Y. F. Yang</name></author><author><name sortKey="Han, J" uniqKey="Han J">J. Han</name></author><author><name sortKey="Mikami, A" uniqKey="Mikami A">A. Mikami</name></author><author><name sortKey="Garza, A E" uniqKey="Garza A">A. E. Garza</name></author><author><name sortKey="Mohanraj, L" uniqKey="Mohanraj L">L Mohanraj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Granata, R" uniqKey="Granata R">R. Granata</name></author><author><name sortKey="Trovato, L" uniqKey="Trovato L">L. Trovato</name></author><author><name sortKey="Garbarino, G" uniqKey="Garbarino G">G. Garbarino</name></author><author><name sortKey="Taliano, M" uniqKey="Taliano M">M. Taliano</name></author><author><name sortKey="Ponti, R" uniqKey="Ponti R">R. Ponti</name></author><author><name sortKey="Sala, G" uniqKey="Sala G">G. Sala</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, B" uniqKey="Liu B">B. Liu</name></author><author><name sortKey="Lee, H Y" uniqKey="Lee H">H. Y. Lee</name></author><author><name sortKey="Weinzimer, S A" uniqKey="Weinzimer S">S. A. Weinzimer</name></author><author><name sortKey="Powell, D R" uniqKey="Powell D">D. R. Powell</name></author><author><name sortKey="Clifford, J L" uniqKey="Clifford J">J. L. Clifford</name></author><author><name sortKey="Kurie, J M" uniqKey="Kurie J">J. M. Kurie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, M Z" uniqKey="Lin M">M. Z. Lin</name></author><author><name sortKey="Marzec, K A" uniqKey="Marzec K">K. A. Marzec</name></author><author><name sortKey="Martin, J L" uniqKey="Martin J">J. L. Martin</name></author><author><name sortKey="Baxter, R C" uniqKey="Baxter R">R. C. Baxter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grkovic, S" uniqKey="Grkovic S">S. Grkovic</name></author><author><name sortKey="O Eilly, V C" uniqKey="O Eilly V">V. C. O’Reilly</name></author><author><name sortKey="Han, S" uniqKey="Han S">S. Han</name></author><author><name sortKey="Hong, M" uniqKey="Hong M">M. Hong</name></author><author><name sortKey="Baxter, R C" uniqKey="Baxter R">R. C. Baxter</name></author><author><name sortKey="Firth, S M" uniqKey="Firth S">S. M. Firth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Y C" uniqKey="Lee Y">Y. C. Lee</name></author><author><name sortKey="Jogie Brahim, S" uniqKey="Jogie Brahim S">S. Jogie-Brahim</name></author><author><name sortKey="Lee, D Y" uniqKey="Lee D">D. Y. Lee</name></author><author><name sortKey="Han, J" uniqKey="Han J">J. Han</name></author><author><name sortKey="Harada, A" uniqKey="Harada A">A. Harada</name></author><author><name sortKey="Murphy, L J" uniqKey="Murphy L">L. J Murphy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Veraldi, K L" uniqKey="Veraldi K">K. L. Veraldi</name></author><author><name sortKey="Gibson, B T" uniqKey="Gibson B">B. T. Gibson</name></author><author><name sortKey="Yasuoka, H" uniqKey="Yasuoka H">H. Yasuoka</name></author><author><name sortKey="Myerburg, M M" uniqKey="Myerburg M">M. M. Myerburg</name></author><author><name sortKey="Kelly, E A" uniqKey="Kelly E">E. A. Kelly</name></author><author><name sortKey="Balzar, S" uniqKey="Balzar S">S. Balzar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rocha, R L" uniqKey="Rocha R">R. L. Rocha</name></author><author><name sortKey="Hilsenbeck, S G" uniqKey="Hilsenbeck S">S. G. Hilsenbeck</name></author><author><name sortKey="Jackson, J G" uniqKey="Jackson J">J. G. Jackson</name></author><author><name sortKey="Lee, A V" uniqKey="Lee A">A. V. Lee</name></author><author><name sortKey="Figueroa, J A" uniqKey="Figueroa J">J. A. Figueroa</name></author><author><name sortKey="Yee, D" uniqKey="Yee D">D. Yee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johnson, M A" uniqKey="Johnson M">M. A. Johnson</name></author><author><name sortKey="Firth, S M" uniqKey="Firth S">S. M. Firth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Y X" uniqKey="Chan Y">Y. X. Chan</name></author><author><name sortKey="Alfonso, H" uniqKey="Alfonso H">H. Alfonso</name></author><author><name sortKey="Paul Chubb, S A" uniqKey="Paul Chubb S">S. A. Paul Chubb</name></author><author><name sortKey="Ho, K K Y" uniqKey="Ho K">K. K. Y. Ho</name></author><author><name sortKey="Gerard Fegan, P" uniqKey="Gerard Fegan P">P. Gerard Fegan</name></author><author><name sortKey="Hankey, G J" uniqKey="Hankey G">G. J. Hankey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Natsuizaka, M" uniqKey="Natsuizaka M">M. Natsuizaka</name></author><author><name sortKey="Kinugasa, H" uniqKey="Kinugasa H">H. Kinugasa</name></author><author><name sortKey="Kagawa, S" uniqKey="Kagawa S">S. Kagawa</name></author><author><name sortKey="Whelan, K A" uniqKey="Whelan K">K. A. Whelan</name></author><author><name sortKey="Naganuma, S" uniqKey="Naganuma S">S. Naganuma</name></author><author><name sortKey="Subramanian, H" uniqKey="Subramanian H">H. Subramanian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Prager, A J" uniqKey="Prager A">A. J. Prager</name></author><author><name sortKey="Peng, C R" uniqKey="Peng C">C. R. Peng</name></author><author><name sortKey="Lita, E" uniqKey="Lita E">E. Lita</name></author><author><name sortKey="Mcnally, D" uniqKey="Mcnally D">D. McNally</name></author><author><name sortKey="Kaushal, A" uniqKey="Kaushal A">A. Kaushal</name></author><author><name sortKey="Sproull, M" uniqKey="Sproull M">M. Sproull</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nour, A M" uniqKey="Nour A">A. M. Nour</name></author><author><name sortKey="Yeung, Y G" uniqKey="Yeung Y">Y. G. Yeung</name></author><author><name sortKey="Santambrogio, L" uniqKey="Santambrogio L">L. Santambrogio</name></author><author><name sortKey="Boyden, E D" uniqKey="Boyden E">E. D. Boyden</name></author><author><name sortKey="Stanley, E R" uniqKey="Stanley E">E. R. Stanley</name></author><author><name sortKey="Brojatsch, J" uniqKey="Brojatsch J">J. Brojatsch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Janes, K A" uniqKey="Janes K">K. A. Janes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nour, A M" uniqKey="Nour A">A. M. Nour</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Wolenski, J" uniqKey="Wolenski J">J. Wolenski</name></author><author><name sortKey="Modis, Y" uniqKey="Modis Y">Y. Modis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, C M" uniqKey="Lee C">C. M. Lee</name></author><author><name sortKey="He, C H" uniqKey="He C">C. H. He</name></author><author><name sortKey="Nour, A M" uniqKey="Nour A">A. M. Nour</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name></author><author><name sortKey="Ma, B" uniqKey="Ma B">B. Ma</name></author><author><name sortKey="Park, J W" uniqKey="Park J">J. W. Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Inglese, J" uniqKey="Inglese J">J. Inglese</name></author><author><name sortKey="Blatchly, R A" uniqKey="Blatchly R">R. A. Blatchly</name></author><author><name sortKey="Benkovic, S J" uniqKey="Benkovic S">S. J. Benkovic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Horibata, S" uniqKey="Horibata S">S. Horibata</name></author><author><name sortKey="Vo, T V" uniqKey="Vo T">T. V. Vo</name></author><author><name sortKey="Subramanian, V" uniqKey="Subramanian V">V. Subramanian</name></author><author><name sortKey="Thompson, P R" uniqKey="Thompson P">P. R. Thompson</name></author><author><name sortKey="Coonrod, S A" uniqKey="Coonrod S">S. A. Coonrod</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roth, D" uniqKey="Roth D">D. Roth</name></author><author><name sortKey="Lynes, E" uniqKey="Lynes E">E. Lynes</name></author><author><name sortKey="Riemer, J" uniqKey="Riemer J">J. Riemer</name></author><author><name sortKey="Hansen, H G" uniqKey="Hansen H">H. G. Hansen</name></author><author><name sortKey="Althaus, N" uniqKey="Althaus N">N. Althaus</name></author><author><name sortKey="Simmen, T" uniqKey="Simmen T">T. Simmen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michelsen, K" uniqKey="Michelsen K">K. Michelsen</name></author><author><name sortKey="Yuan, H" uniqKey="Yuan H">H. Yuan</name></author><author><name sortKey="Schwappach, B" uniqKey="Schwappach B">B. Schwappach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ritchie, T K" uniqKey="Ritchie T">T. K. Ritchie</name></author><author><name sortKey="Grinkova, Y V" uniqKey="Grinkova Y">Y. V. Grinkova</name></author><author><name sortKey="Bayburt, T H" uniqKey="Bayburt T">T. H. Bayburt</name></author><author><name sortKey="Denisov, I G" uniqKey="Denisov I">I. G. Denisov</name></author><author><name sortKey="Zolnerciks, J K" uniqKey="Zolnerciks J">J. K. Zolnerciks</name></author><author><name sortKey="Atkins, W M" uniqKey="Atkins W">W. M. Atkins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heyduk, T" uniqKey="Heyduk T">T. Heyduk</name></author><author><name sortKey="Ma, Y" uniqKey="Ma Y">Y. Ma</name></author><author><name sortKey="Tang, H" uniqKey="Tang H">H. Tang</name></author><author><name sortKey="Ebright, R H" uniqKey="Ebright R">R. H. Ebright</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yap, K L" uniqKey="Yap K">K. L. Yap</name></author><author><name sortKey="Ames, J B" uniqKey="Ames J">J. B. Ames</name></author><author><name sortKey="Swindells, M B" uniqKey="Swindells M">M. B. Swindells</name></author><author><name sortKey="Ikura, M" uniqKey="Ikura M">M. Ikura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ricort, J M" uniqKey="Ricort J">J. M. Ricort</name></author><author><name sortKey="Lombet, A" uniqKey="Lombet A">A. Lombet</name></author><author><name sortKey="Lassarre, C" uniqKey="Lassarre C">C. Lassarre</name></author><author><name sortKey="Binoux, M" uniqKey="Binoux M">M. Binoux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, X" uniqKey="Li X">X. Li</name></author><author><name sortKey="Wu, X Q" uniqKey="Wu X">X. Q. Wu</name></author><author><name sortKey="Deng, R" uniqKey="Deng R">R. Deng</name></author><author><name sortKey="Li, D D" uniqKey="Li D">D. D. Li</name></author><author><name sortKey="Tang, J" uniqKey="Tang J">J. Tang</name></author><author><name sortKey="Chen, W D" uniqKey="Chen W">W. D. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zalckvar, E" uniqKey="Zalckvar E">E. Zalckvar</name></author><author><name sortKey="Berissi, H" uniqKey="Berissi H">H. Berissi</name></author><author><name sortKey="Mizrachy, L" uniqKey="Mizrachy L">L. Mizrachy</name></author><author><name sortKey="Idelchuk, Y" uniqKey="Idelchuk Y">Y. Idelchuk</name></author><author><name sortKey="Koren, I" uniqKey="Koren I">I. Koren</name></author><author><name sortKey="Eisenstein, M" uniqKey="Eisenstein M">M. Eisenstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zalckvar, E" uniqKey="Zalckvar E">E. Zalckvar</name></author><author><name sortKey="Berissi, H" uniqKey="Berissi H">H. Berissi</name></author><author><name sortKey="Eisenstein, M" uniqKey="Eisenstein M">M. Eisenstein</name></author><author><name sortKey="Kimchi, A" uniqKey="Kimchi A">A. Kimchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pfisterer, S G" uniqKey="Pfisterer S">S. G. Pfisterer</name></author><author><name sortKey="Mauthe, M" uniqKey="Mauthe M">M. Mauthe</name></author><author><name sortKey="Codogno, P" uniqKey="Codogno P">P. Codogno</name></author><author><name sortKey="Proikas Cezanne, T" uniqKey="Proikas Cezanne T">T. Proikas-Cezanne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Howell, G M" uniqKey="Howell G">G. M. Howell</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L. Guo</name></author><author><name sortKey="Collage, R D" uniqKey="Collage R">R. D. Collage</name></author><author><name sortKey="Loughran, P A" uniqKey="Loughran P">P. A. Loughran</name></author><author><name sortKey="Zuckerbraun, B S" uniqKey="Zuckerbraun B">B. S. Zuckerbraun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Andreev, O A" uniqKey="Andreev O">O. A. Andreev</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D. M. Engelman</name></author><author><name sortKey="Reshetnyak, Y K" uniqKey="Reshetnyak Y">Y. K. Reshetnyak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deacon, J C" uniqKey="Deacon J">J. C. Deacon</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D. M. Engelman</name></author><author><name sortKey="Barrera, F N" uniqKey="Barrera F">F. N. Barrera</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wijesinghe, D" uniqKey="Wijesinghe D">D. Wijesinghe</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D. M. Engelman</name></author><author><name sortKey="Andreev, O A" uniqKey="Andreev O">O. A. Andreev</name></author><author><name sortKey="Reshetnyak, Y K" uniqKey="Reshetnyak Y">Y. K. Reshetnyak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zoonens, M" uniqKey="Zoonens M">M. Zoonens</name></author><author><name sortKey="Reshetnyak, Y K" uniqKey="Reshetnyak Y">Y. K. Reshetnyak</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D. M. Engelman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Czogalla, K J" uniqKey="Czogalla K">K. J. Czogalla</name></author><author><name sortKey="Biswas, A" uniqKey="Biswas A">A. Biswas</name></author><author><name sortKey="Rost, S" uniqKey="Rost S">S. Rost</name></author><author><name sortKey="Watzka, M" uniqKey="Watzka M">M. Watzka</name></author><author><name sortKey="Oldenburg, J" uniqKey="Oldenburg J">J. Oldenburg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zerangue, N" uniqKey="Zerangue N">N. Zerangue</name></author><author><name sortKey="Schwappach, B" uniqKey="Schwappach B">B. Schwappach</name></author><author><name sortKey="Jan, Y N" uniqKey="Jan Y">Y. N. Jan</name></author><author><name sortKey="Jan, L Y" uniqKey="Jan L">L. Y. Jan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="O Elly, I" uniqKey="O Elly I">I. O’Kelly</name></author><author><name sortKey="Butler, M H" uniqKey="Butler M">M. H. Butler</name></author><author><name sortKey="Zilberberg, N" uniqKey="Zilberberg N">N. Zilberberg</name></author><author><name sortKey="Goldstein, S A" uniqKey="Goldstein S">S. A. Goldstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, H" uniqKey="Yuan H">H. Yuan</name></author><author><name sortKey="Michelsen, K" uniqKey="Michelsen K">K. Michelsen</name></author><author><name sortKey="Schwappach, B" uniqKey="Schwappach B">B. Schwappach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miller, S G" uniqKey="Miller S">S. G. Miller</name></author><author><name sortKey="Kennedy, M B" uniqKey="Kennedy M">M. B. Kennedy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez Mora, O G" uniqKey="Rodriguez Mora O">O. G. Rodriguez-Mora</name></author><author><name sortKey="Lahair, M M" uniqKey="Lahair M">M. M. Lahair</name></author><author><name sortKey="Evans, M J" uniqKey="Evans M">M. J. Evans</name></author><author><name sortKey="Kovacs, C J" uniqKey="Kovacs C">C. J. Kovacs</name></author><author><name sortKey="Allison, R R" uniqKey="Allison R">R. R. Allison</name></author><author><name sortKey="Sibata, C H" uniqKey="Sibata C">C. H. Sibata</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sheaves, R M" uniqKey="Sheaves R">R. M. Sheaves</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y. Yang</name></author><author><name sortKey="Ma, F" uniqKey="Ma F">F. Ma</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z. Liu</name></author><author><name sortKey="Su, Q" uniqKey="Su Q">Q. Su</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Ni, M" uniqKey="Ni M">M. Ni</name></author><author><name sortKey="Lee, B" uniqKey="Lee B">B. Lee</name></author><author><name sortKey="Barron, E" uniqKey="Barron E">E. Barron</name></author><author><name sortKey="Hinton, D R" uniqKey="Hinton D">D. R. Hinton</name></author><author><name sortKey="Lee, A S" uniqKey="Lee A">A. S. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sosunov, E A" uniqKey="Sosunov E">E. A. Sosunov</name></author><author><name sortKey="Anyukhovsky, E P" uniqKey="Anyukhovsky E">E. P. Anyukhovsky</name></author><author><name sortKey="Sosunov, A A" uniqKey="Sosunov A">A. A. Sosunov</name></author><author><name sortKey="Moshnikova, A" uniqKey="Moshnikova A">A. Moshnikova</name></author><author><name sortKey="Wijesinghe, D" uniqKey="Wijesinghe D">D. Wijesinghe</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D. M. Engelman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yao, L" uniqKey="Yao L">L. Yao</name></author><author><name sortKey="Daniels, J" uniqKey="Daniels J">J. Daniels</name></author><author><name sortKey="Moshnikova, A" uniqKey="Moshnikova A">A. Moshnikova</name></author><author><name sortKey="Kuznetsov, S" uniqKey="Kuznetsov S">S. Kuznetsov</name></author><author><name sortKey="Ahmed, A" uniqKey="Ahmed A">A. Ahmed</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D. M. Engelman</name></author><author><name sortKey="Reshetnyak, Y K" uniqKey="Reshetnyak Y">Y. K. Reshetnyak</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31220095</article-id><article-id pub-id-type="pmc">6586287</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0218091</article-id><article-id pub-id-type="publisher-id">PONE-D-18-35301</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cell Processes</subject><subj-group><subject>Cell Death</subject><subj-group><subject>Autophagic Cell Death</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Biological Cultures</subject><subj-group><subject>Cell Lines</subject><subj-group><subject>Vero Cells</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Structures and Organelles</subject><subj-group><subject>Cell Membranes</subject><subj-group><subject>Membrane Proteins</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Specimen Preparation and Treatment</subject><subj-group><subject>Staining</subject><subj-group><subject>Cell Staining</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>Post-Translational Modification</subject><subj-group><subject>Signal Peptides</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Oncology</subject><subj-group><subject>Cancers and Neoplasms</subject><subj-group><subject>Breast Tumors</subject><subj-group><subject>Breast Cancer</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>Intracellular Receptors</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Signal Transduction</subject><subj-group><subject>Intracellular Receptors</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Structures and Organelles</subject><subj-group><subject>Endoplasmic Reticulum</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cell Processes</subject><subj-group><subject>Secretory Pathway</subject><subj-group><subject>Endoplasmic Reticulum</subject></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Blocking transmembrane219 protein signaling inhibits autophagy and restores normal cell death</article-title><alt-title alt-title-type="running-head">Interrupting transmembrane219 protein signaling blocks autophagy and induces cell death</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Joyce</surname><given-names>Sean</given-names></name><role content-type="http://credit.casrai.org/">Data curation</role><role content-type="http://credit.casrai.org/">Investigation</role><role content-type="http://credit.casrai.org/">Writing – review & editing</role><xref ref-type="aff" rid="aff001"></xref></contrib><contrib contrib-type="author"><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0003-2187-1801</contrib-id><name><surname>Nour</surname><given-names>Adel M.</given-names></name><role content-type="http://credit.casrai.org/">Conceptualization</role><role content-type="http://credit.casrai.org/">Data curation</role><role content-type="http://credit.casrai.org/">Formal analysis</role><role content-type="http://credit.casrai.org/">Investigation</role><role content-type="http://credit.casrai.org/">Supervision</role><role content-type="http://credit.casrai.org/">Validation</role><role content-type="http://credit.casrai.org/">Visualization</role><role content-type="http://credit.casrai.org/">Writing – original draft</role><role content-type="http://credit.casrai.org/">Writing – review & editing</role><xref ref-type="aff" rid="aff001"></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><addr-line>Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Trajkovic</surname><given-names>Vladimir</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Univerzitet u Beogradu, SERBIA</addr-line></aff><author-notes><fn fn-type="COI-statement" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><corresp id="cor001">* E-mail: <email>adel_m_nour@brown.edu</email></corresp></author-notes><pub-date pub-type="epub"><day>20</day><month>6</month><year>2019</year></pub-date><pub-date pub-type="collection"><year>2019</year></pub-date><volume>14</volume><issue>6</issue><elocation-id>e0218091</elocation-id><history><date date-type="received"><day>10</day><month>12</month><year>2018</year></date><date date-type="accepted"><day>24</day><month>5</month><year>2019</year></date></history><permissions><copyright-statement>© 2019 Joyce, Nour</copyright-statement><copyright-year>2019</copyright-year><copyright-holder>Joyce, Nour</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0218091.pdf"></self-uri><abstract><p>Autophagy plays a vital role in tumor therapy and survival of dormant tumor cells. Here we describe a novel function of a protein known as Transmembrane 219 (TM219) as an autophagy activator. TM219 is a small membrane protein expressed in all known human tissues except the thymus. We used biochemical approaches to identify calmodulin and calmodulin dependent protein kinase II as a part of TM219 protein complex. Then, we employed <italic>in vitro</italic> reconstitution system and fluorescence anisotropy to study the requirements of TM219 to bind calmodulin <italic>in vitro</italic>. We also used this system to study the effects of a synthetic peptide derived from the sequence of the short cytoplasmic tail of TM219 (SCTT) on calmodulin-TM219 receptor interactions. We conjugated SCTT peptide with a pH Low Insertion peptide (pHLIP) for optimal cellular delivery. We finally tested the effects of SCTT-pHLIP on triple negative human breast cancer cells in three dimension culture. Our data defined a novel function of TM219 protein and an efficient approach to inhibit it.</p></abstract><funding-group><funding-statement>The authors received no specific funding for this work.</funding-statement></funding-group><counts><fig-count count="7"></fig-count><table-count count="2"></table-count><page-count count="25"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the manuscript and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the manuscript and its Supporting Information files.</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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