Forkhead box K1 regulates the malignant behavior of gastric cancer by inhibiting autophagy
Identifieur interne : 000359 ( Pmc/Corpus ); précédent : 000358; suivant : 000360Forkhead box K1 regulates the malignant behavior of gastric cancer by inhibiting autophagy
Auteurs : Yixuan Wang ; Wensheng Qiu ; Ning Liu ; Libin Sun ; Zhao Liu ; Shasha Wang ; Peng Wang ; Shihai Liu ; Jing LvSource :
- Annals of Translational Medicine [ 2305-5839 ] ; 2020.
Abstract
Forkhead box K1 (FOXK1) is a transcription factor that contributes to cancer development, but it is unclear how FOXK1 regulates the proliferation and migration of gastric cancer (GC) cells. The purpose of this study was to investigate the clinical significance, biological function, and molecular mechanisms of FOXK1 in GC.
We conducted bioinformatics assays and western blotting to assess FOXK1 expression. Then, we performed immunohistochemistry (IHC) with tissue microarrays (TMAs) to assess FOXK1 expression in order to identify an association between FOXK1 expression levels and clinical parameters. We used 5-ethynyl-2’-deoxyuridine (EdU), wound healing and Transwell assays to determine whether FOXK1 promotes malignant behaviors in GC. Furthermore, immunofluorescence staining, transmission electron microscopy and western blotting were used to verify an association between FOXK1 and autophagy.
We observed high levels of FOXK1 expression in GC tissues, which were associated with the degree of malignancy in GC. FOXK1 promotes the malignant behavior of GC by regulating autophagy via activation of the class I phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway and inhibition of the expression of class III PI3K.
These findings provide a new target for the comprehensive treatment of GC by highlighting the relationship between FOXK1 and malignant behaviors in GC.
Url:
DOI: 10.21037/atm.2019.12.123
PubMed: 32175400
PubMed Central: 7049027
Links to Exploration step
PMC:7049027Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Forkhead box K1 regulates the malignant behavior of gastric cancer by inhibiting autophagy</title>
<author><name sortKey="Wang, Yixuan" sort="Wang, Yixuan" uniqKey="Wang Y" first="Yixuan" last="Wang">Yixuan Wang</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Qiu, Wensheng" sort="Qiu, Wensheng" uniqKey="Qiu W" first="Wensheng" last="Qiu">Wensheng Qiu</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Ning" sort="Liu, Ning" uniqKey="Liu N" first="Ning" last="Liu">Ning Liu</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sun, Libin" sort="Sun, Libin" uniqKey="Sun L" first="Libin" last="Sun">Libin Sun</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Zhao" sort="Liu, Zhao" uniqKey="Liu Z" first="Zhao" last="Liu">Zhao Liu</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Shasha" sort="Wang, Shasha" uniqKey="Wang S" first="Shasha" last="Wang">Shasha Wang</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Peng" sort="Wang, Peng" uniqKey="Wang P" first="Peng" last="Wang">Peng Wang</name>
<affiliation><nlm:aff id="aff2">Department of Oncology, Weifang Yidu Central Hospital, Qingzhou<addr-line>262500,<country>China</country>
</addr-line>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Shihai" sort="Liu, Shihai" uniqKey="Liu S" first="Shihai" last="Liu">Shihai Liu</name>
<affiliation><nlm:aff id="aff3">Central Laboratory,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lv, Jing" sort="Lv, Jing" uniqKey="Lv J" first="Jing" last="Lv">Jing Lv</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">32175400</idno>
<idno type="pmc">7049027</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049027</idno>
<idno type="RBID">PMC:7049027</idno>
<idno type="doi">10.21037/atm.2019.12.123</idno>
<date when="2020">2020</date>
<idno type="wicri:Area/Pmc/Corpus">000359</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000359</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Forkhead box K1 regulates the malignant behavior of gastric cancer by inhibiting autophagy</title>
<author><name sortKey="Wang, Yixuan" sort="Wang, Yixuan" uniqKey="Wang Y" first="Yixuan" last="Wang">Yixuan Wang</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Qiu, Wensheng" sort="Qiu, Wensheng" uniqKey="Qiu W" first="Wensheng" last="Qiu">Wensheng Qiu</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Ning" sort="Liu, Ning" uniqKey="Liu N" first="Ning" last="Liu">Ning Liu</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sun, Libin" sort="Sun, Libin" uniqKey="Sun L" first="Libin" last="Sun">Libin Sun</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Zhao" sort="Liu, Zhao" uniqKey="Liu Z" first="Zhao" last="Liu">Zhao Liu</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Shasha" sort="Wang, Shasha" uniqKey="Wang S" first="Shasha" last="Wang">Shasha Wang</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Peng" sort="Wang, Peng" uniqKey="Wang P" first="Peng" last="Wang">Peng Wang</name>
<affiliation><nlm:aff id="aff2">Department of Oncology, Weifang Yidu Central Hospital, Qingzhou<addr-line>262500,<country>China</country>
</addr-line>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Shihai" sort="Liu, Shihai" uniqKey="Liu S" first="Shihai" last="Liu">Shihai Liu</name>
<affiliation><nlm:aff id="aff3">Central Laboratory,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lv, Jing" sort="Lv, Jing" uniqKey="Lv J" first="Jing" last="Lv">Jing Lv</name>
<affiliation><nlm:aff id="aff1">Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Annals of Translational Medicine</title>
<idno type="ISSN">2305-5839</idno>
<idno type="eISSN">2305-5847</idno>
<imprint><date when="2020">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Forkhead box K1 (FOXK1) is a transcription factor that contributes to cancer development, but it is unclear how FOXK1 regulates the proliferation and migration of gastric cancer (GC) cells. The purpose of this study was to investigate the clinical significance, biological function, and molecular mechanisms of FOXK1 in GC.</p>
</sec>
<sec><title>Methods</title>
<p>We conducted bioinformatics assays and western blotting to assess FOXK1 expression. Then, we performed immunohistochemistry (IHC) with tissue microarrays (TMAs) to assess FOXK1 expression in order to identify an association between FOXK1 expression levels and clinical parameters. We used 5-ethynyl-2’-deoxyuridine (EdU), wound healing and Transwell assays to determine whether FOXK1 promotes malignant behaviors in GC. Furthermore, immunofluorescence staining, transmission electron microscopy and western blotting were used to verify an association between FOXK1 and autophagy.</p>
</sec>
<sec><title>Results</title>
<p>We observed high levels of FOXK1 expression in GC tissues, which were associated with the degree of malignancy in GC. FOXK1 promotes the malignant behavior of GC by regulating autophagy via activation of the class I phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway and inhibition of the expression of class III PI3K.</p>
</sec>
<sec><title>Conclusions</title>
<p>These findings provide a new target for the comprehensive treatment of GC by highlighting the relationship between FOXK1 and malignant behaviors in GC.</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Ann Transl Med</journal-id>
<journal-id journal-id-type="iso-abbrev">Ann Transl Med</journal-id>
<journal-id journal-id-type="publisher-id">ATM</journal-id>
<journal-title-group><journal-title>Annals of Translational Medicine</journal-title>
</journal-title-group>
<issn pub-type="ppub">2305-5839</issn>
<issn pub-type="epub">2305-5847</issn>
<publisher><publisher-name>AME Publishing Company</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">32175400</article-id>
<article-id pub-id-type="pmc">7049027</article-id>
<article-id pub-id-type="publisher-id">atm-08-04-107</article-id>
<article-id pub-id-type="doi">10.21037/atm.2019.12.123</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Forkhead box K1 regulates the malignant behavior of gastric cancer by inhibiting autophagy</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Yixuan</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="afn2"><sup>#</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qiu</surname>
<given-names>Wensheng</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="afn2"><sup>#</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Ning</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sun</surname>
<given-names>Libin</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Zhao</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Shasha</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Peng</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Liu</surname>
<given-names>Shihai</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Lv</surname>
<given-names>Jing</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<aff id="aff1"><label>1</label>
Department of Oncology,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
;</aff>
<aff id="aff2"><label>2</label>
Department of Oncology, Weifang Yidu Central Hospital, Qingzhou<addr-line>262500,<country>China</country>
</addr-line>
;</aff>
<aff id="aff3"><label>3</label>
Central Laboratory,<institution>the Affiliated Hospital of Qingdao University</institution>
,<addr-line>Qingdao 266071</addr-line>
,<country>China</country>
</aff>
</contrib-group>
<author-notes><fn id="afn1"><p><italic>Contributions:</italic>
(I) Conception and design: Y Wang; (II) Administrative support: W Qiu, S Liu, J Lv; (III) Provision of study materials or patients: All authors; (IV) Collection and assembly of data: All authors; (V) Data analysis and interpretation: All authors; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.</p>
</fn>
<fn id="afn2" fn-type="equal"><label>#</label>
<p>These authors contributed equally to this work.</p>
</fn>
<corresp id="cor1"><italic>Correspondence to:</italic>
Dr. Jing Lv. Department of Oncology, the Affiliated Hospital of Qingdao University, Qingdao 266071, China. Email: <email xlink:href="lvjing8291@126.com">lvjing8291@126.com</email>
; Dr. Shihai Liu. Central Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266071, China. Email: <email xlink:href="shliumed@126.com">shliumed@126.com</email>
.</corresp>
<fn fn-type="COI-statement"><p><italic>Conflicts of Interest:</italic>
The authors declare no conflicts of interest.</p>
</fn>
</author-notes>
<pub-date pub-type="epub-ppub"><month>2</month>
<year>2020</year>
</pub-date>
<pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>2</month>
<year>2020</year>
</pub-date>
<volume>8</volume>
<issue>4</issue>
<elocation-id>107</elocation-id>
<history><date date-type="received"><day>15</day>
<month>8</month>
<year>2019</year>
</date>
<date date-type="accepted"><day>29</day>
<month>11</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>2020 Annals of Translational Medicine. All rights reserved.</copyright-statement>
<copyright-year>2020</copyright-year>
<copyright-holder>Annals of Translational Medicine.</copyright-holder>
</permissions>
<abstract><sec><title>Background</title>
<p>Forkhead box K1 (FOXK1) is a transcription factor that contributes to cancer development, but it is unclear how FOXK1 regulates the proliferation and migration of gastric cancer (GC) cells. The purpose of this study was to investigate the clinical significance, biological function, and molecular mechanisms of FOXK1 in GC.</p>
</sec>
<sec><title>Methods</title>
<p>We conducted bioinformatics assays and western blotting to assess FOXK1 expression. Then, we performed immunohistochemistry (IHC) with tissue microarrays (TMAs) to assess FOXK1 expression in order to identify an association between FOXK1 expression levels and clinical parameters. We used 5-ethynyl-2’-deoxyuridine (EdU), wound healing and Transwell assays to determine whether FOXK1 promotes malignant behaviors in GC. Furthermore, immunofluorescence staining, transmission electron microscopy and western blotting were used to verify an association between FOXK1 and autophagy.</p>
</sec>
<sec><title>Results</title>
<p>We observed high levels of FOXK1 expression in GC tissues, which were associated with the degree of malignancy in GC. FOXK1 promotes the malignant behavior of GC by regulating autophagy via activation of the class I phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway and inhibition of the expression of class III PI3K.</p>
</sec>
<sec><title>Conclusions</title>
<p>These findings provide a new target for the comprehensive treatment of GC by highlighting the relationship between FOXK1 and malignant behaviors in GC.</p>
</sec>
</abstract>
<kwd-group kwd-group-type="author"><title>Keywords: </title>
<kwd>Forkhead box K1 (FOXK1)</kwd>
<kwd>autophagy</kwd>
<kwd>gastric cancer</kwd>
<kwd>3-methyladenine (3-MA)</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000359 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000359 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Pmc |étape= Corpus |type= RBID |clé= PMC:7049027 |texte= Forkhead box K1 regulates the malignant behavior of gastric cancer by inhibiting autophagy }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:32175400" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a ChloroquineV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |