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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cordycepin inhibits human ovarian cancer by inducing autophagy and apoptosis through Dickkopf-related protein 1/β-catenin signaling</title><author><name sortKey="Jang, Hyun Jin" sort="Jang, Hyun Jin" uniqKey="Jang H" first="Hyun-Jin" last="Jang">Hyun-Jin Jang</name><affiliation><nlm:aff id="au1"><institution>Division of Bioconvergence Analysis, Korea Basic Science Institute</institution><addr-line>Daejeon 305-333, Republic of Korea</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au2"><institution>Department of Biological Sciences, Sungkyunkwan University</institution><addr-line>Suwon 16419, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yang, Kyeong Eun" sort="Yang, Kyeong Eun" uniqKey="Yang K" first="Kyeong Eun" last="Yang">Kyeong Eun Yang</name><affiliation><nlm:aff id="au1"><institution>Division of Bioconvergence Analysis, Korea Basic Science Institute</institution><addr-line>Daejeon 305-333, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hwang, In Hu" sort="Hwang, In Hu" uniqKey="Hwang I" first="In-Hu" last="Hwang">In-Hu Hwang</name><affiliation><nlm:aff id="au3"><institution>Neuroscience Research Institute, Korea University College of Medicine</institution><addr-line>Seoul 136-705, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Huh, Yang Hoon" sort="Huh, Yang Hoon" uniqKey="Huh Y" first="Yang Hoon" last="Huh">Yang Hoon Huh</name><affiliation><nlm:aff id="au4"><institution>Electron Microscopy Research Center, Korea Basic Science Institute</institution><addr-line>Cheongju 28119, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Dae Joon" sort="Kim, Dae Joon" uniqKey="Kim D" first="Dae Joon" last="Kim">Dae Joon Kim</name><affiliation><nlm:aff id="au5"><institution>Department of Biomedical Sciences, School of Medicine, University of Texas Rio Grande Valley</institution><addr-line>Edinburg, Texas, USA</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yoo, Hwa Seung" sort="Yoo, Hwa Seung" uniqKey="Yoo H" first="Hwa-Seung" last="Yoo">Hwa-Seung Yoo</name><affiliation><nlm:aff id="au6"><institution>East-West Cancer Center, Daejeon University</institution><addr-line>Daejeon 302-120, Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Park, Soo Jung" sort="Park, Soo Jung" uniqKey="Park S" first="Soo Jung" last="Park">Soo Jung Park</name><affiliation><nlm:aff id="au7"><institution>Department of Sasang Constitutional Medicine, College of Korean Medicine, Woosuk University</institution><addr-line>Wanju, Jeonbuk 55338, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Jang, Ik Soon" sort="Jang, Ik Soon" uniqKey="Jang I" first="Ik-Soon" last="Jang">Ik-Soon Jang</name><affiliation><nlm:aff id="au1"><institution>Division of Bioconvergence Analysis, Korea Basic Science Institute</institution><addr-line>Daejeon 305-333, Republic of Korea</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au8"><institution>Division of Analytical Science, University of Science and Technology</institution><addr-line>Daejeon 34113, Republic of Korea</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31814895</idno><idno type="pmc">6895532</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895532</idno><idno type="RBID">PMC:6895532</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000357</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000357</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cordycepin inhibits human ovarian cancer by inducing autophagy and apoptosis through Dickkopf-related protein 1/β-catenin signaling</title><author><name sortKey="Jang, Hyun Jin" sort="Jang, Hyun Jin" uniqKey="Jang H" first="Hyun-Jin" last="Jang">Hyun-Jin Jang</name><affiliation><nlm:aff id="au1"><institution>Division of Bioconvergence Analysis, Korea Basic Science Institute</institution><addr-line>Daejeon 305-333, Republic of Korea</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au2"><institution>Department of Biological Sciences, Sungkyunkwan University</institution><addr-line>Suwon 16419, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yang, Kyeong Eun" sort="Yang, Kyeong Eun" uniqKey="Yang K" first="Kyeong Eun" last="Yang">Kyeong Eun Yang</name><affiliation><nlm:aff id="au1"><institution>Division of Bioconvergence Analysis, Korea Basic Science Institute</institution><addr-line>Daejeon 305-333, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hwang, In Hu" sort="Hwang, In Hu" uniqKey="Hwang I" first="In-Hu" last="Hwang">In-Hu Hwang</name><affiliation><nlm:aff id="au3"><institution>Neuroscience Research Institute, Korea University College of Medicine</institution><addr-line>Seoul 136-705, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Huh, Yang Hoon" sort="Huh, Yang Hoon" uniqKey="Huh Y" first="Yang Hoon" last="Huh">Yang Hoon Huh</name><affiliation><nlm:aff id="au4"><institution>Electron Microscopy Research Center, Korea Basic Science Institute</institution><addr-line>Cheongju 28119, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Dae Joon" sort="Kim, Dae Joon" uniqKey="Kim D" first="Dae Joon" last="Kim">Dae Joon Kim</name><affiliation><nlm:aff id="au5"><institution>Department of Biomedical Sciences, School of Medicine, University of Texas Rio Grande Valley</institution><addr-line>Edinburg, Texas, USA</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yoo, Hwa Seung" sort="Yoo, Hwa Seung" uniqKey="Yoo H" first="Hwa-Seung" last="Yoo">Hwa-Seung Yoo</name><affiliation><nlm:aff id="au6"><institution>East-West Cancer Center, Daejeon University</institution><addr-line>Daejeon 302-120, Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Park, Soo Jung" sort="Park, Soo Jung" uniqKey="Park S" first="Soo Jung" last="Park">Soo Jung Park</name><affiliation><nlm:aff id="au7"><institution>Department of Sasang Constitutional Medicine, College of Korean Medicine, Woosuk University</institution><addr-line>Wanju, Jeonbuk 55338, Republic of Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Jang, Ik Soon" sort="Jang, Ik Soon" uniqKey="Jang I" first="Ik-Soon" last="Jang">Ik-Soon Jang</name><affiliation><nlm:aff id="au1"><institution>Division of Bioconvergence Analysis, Korea Basic Science Institute</institution><addr-line>Daejeon 305-333, Republic of Korea</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au8"><institution>Division of Analytical Science, University of Science and Technology</institution><addr-line>Daejeon 34113, Republic of Korea</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">American Journal of Translational Research</title><idno type="eISSN">1943-8141</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Cordycepin, the major active component from <italic>Cordyceps militaris</italic>, has been reported to significantly inhibit some types of cancer; however, its effects on ovarian cancer are still not well understood. In this study, we treated human ovarian cancer cells with different doses of cordycepin and found that it dose-dependently reduced ovarian cancer cell viability, based on Cell counting kit-8 reagent. Immunoblotting showed that cordycepin increased Dickkopf-related protein 1 (Dkk1) levels and inhibited β-catenin signaling. Atg7 knockdown in ovarian cancer cells significantly inhibited cordycepin-induced apoptosis, whereas β-catenin overexpression abolished the effects of cordycepin on cell death and proliferation. Furthermore, we found that Dkk1 overexpression by transfection downregulated the expression of c-Myc and cyclin D1. siRNA-mediated Dkk1 silencing downregulated the expression of Atg8, beclin, and LC3 and promoted β-catenin translocation from the cytoplasm into the nucleus. These results suggest that cordycepin inhibits ovarian cancer cell growth, possibly through coordinated autophagy and Dkk1/β-catenin signaling. Taken together, our findings provide new insights into the treatment of ovarian cancer using cordycepin.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Am J Transl Res</journal-id><journal-id journal-id-type="iso-abbrev">Am J Transl Res</journal-id><journal-id journal-id-type="publisher-id">ajtr</journal-id><journal-title-group><journal-title>American Journal of Translational Research</journal-title></journal-title-group><issn pub-type="epub">1943-8141</issn><publisher><publisher-name>e-Century Publishing Corporation</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31814895</article-id><article-id pub-id-type="pmc">6895532</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Cordycepin inhibits human ovarian cancer by inducing autophagy and apoptosis through Dickkopf-related protein 1/β-catenin signaling</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Jang</surname><given-names>Hyun-Jin</given-names></name><xref ref-type="aff" rid="au1">1</xref><xref ref-type="aff" rid="au2">2</xref><xref ref-type="author-notes" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Kyeong Eun</given-names></name><xref ref-type="aff" rid="au1">1</xref><xref ref-type="author-notes" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Hwang</surname><given-names>In-Hu</given-names></name><xref ref-type="aff" rid="au3">3</xref></contrib><contrib contrib-type="author"><name><surname>Huh</surname><given-names>Yang Hoon</given-names></name><xref ref-type="aff" rid="au4">4</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Dae Joon</given-names></name><xref ref-type="aff" rid="au5">5</xref></contrib><contrib contrib-type="author"><name><surname>Yoo</surname><given-names>Hwa-Seung</given-names></name><xref ref-type="aff" rid="au6">6</xref></contrib><contrib contrib-type="author"><name><surname>Park</surname><given-names>Soo Jung</given-names></name><xref ref-type="aff" rid="au7">7</xref></contrib><contrib contrib-type="author"><name><surname>Jang</surname><given-names>Ik-Soon</given-names></name><xref ref-type="aff" rid="au1">1</xref><xref ref-type="aff" rid="au8">8</xref></contrib><aff id="au1"><label>1</label><institution>Division of Bioconvergence Analysis, Korea Basic Science Institute</institution><addr-line>Daejeon 305-333, Republic of Korea</addr-line></aff><aff id="au2"><label>2</label><institution>Department of Biological Sciences, Sungkyunkwan University</institution><addr-line>Suwon 16419, Republic of Korea</addr-line></aff><aff id="au3"><label>3</label><institution>Neuroscience Research Institute, Korea University College of Medicine</institution><addr-line>Seoul 136-705, Republic of Korea</addr-line></aff><aff id="au4"><label>4</label><institution>Electron Microscopy Research Center, Korea Basic Science Institute</institution><addr-line>Cheongju 28119, Republic of Korea</addr-line></aff><aff id="au5"><label>5</label><institution>Department of Biomedical Sciences, School of Medicine, University of Texas Rio Grande Valley</institution><addr-line>Edinburg, Texas, USA</addr-line></aff><aff id="au6"><label>6</label><institution>East-West Cancer Center, Daejeon University</institution><addr-line>Daejeon 302-120, Korea</addr-line></aff><aff id="au7"><label>7</label><institution>Department of Sasang Constitutional Medicine, College of Korean Medicine, Woosuk University</institution><addr-line>Wanju, Jeonbuk 55338, Republic of Korea</addr-line></aff><aff id="au8"><label>8</label><institution>Division of Analytical Science, University of Science and Technology</institution><addr-line>Daejeon 34113, Republic of Korea</addr-line></aff></contrib-group><author-notes><corresp><bold>Address correspondence to:</bold> Dr. Ik-Soon Jang, Division of Bioconvergence Analysis, Korea Basic Science Institute, Gwahangno 113, Yuseong-gu, Daejeon 305-333, Republic of Korea. Tel: +82-865-3430; Fax: +82-865-3419; E-mail: <email>jangiksn@kbsi.re.kr</email>; Soo Jung Park, Department of Sasang Constitutional Medicine, College of Korean Medicine, Woosuk University, Wanju, Jeonbuk 55338, Republic of Korea. Tel: +82-010-6287-0248; E-mail: <email>taorgi@daum.net</email></corresp><fn id="fn1"><label>*</label><p>Equal contributors.</p></fn></author-notes><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>15</day><month>11</month><year>2019</year></pub-date><volume>11</volume><issue>11</issue><fpage>6890</fpage><lpage>6906</lpage><history><date date-type="received"><day>27</day><month>5</month><year>2019</year></date><date date-type="accepted"><day>31</day><month>7</month><year>2019</year></date></history><permissions><copyright-statement>AJTR Copyright © 2019</copyright-statement><copyright-year>2019</copyright-year></permissions><abstract><p>Cordycepin, the major active component from <italic>Cordyceps militaris</italic>, has been reported to significantly inhibit some types of cancer; however, its effects on ovarian cancer are still not well understood. In this study, we treated human ovarian cancer cells with different doses of cordycepin and found that it dose-dependently reduced ovarian cancer cell viability, based on Cell counting kit-8 reagent. Immunoblotting showed that cordycepin increased Dickkopf-related protein 1 (Dkk1) levels and inhibited β-catenin signaling. Atg7 knockdown in ovarian cancer cells significantly inhibited cordycepin-induced apoptosis, whereas β-catenin overexpression abolished the effects of cordycepin on cell death and proliferation. Furthermore, we found that Dkk1 overexpression by transfection downregulated the expression of c-Myc and cyclin D1. siRNA-mediated Dkk1 silencing downregulated the expression of Atg8, beclin, and LC3 and promoted β-catenin translocation from the cytoplasm into the nucleus. These results suggest that cordycepin inhibits ovarian cancer cell growth, possibly through coordinated autophagy and Dkk1/β-catenin signaling. Taken together, our findings provide new insights into the treatment of ovarian cancer using cordycepin.</p></abstract><kwd-group><kwd>Apoptosis</kwd><kwd>Dickkopf-related protein 1</kwd><kwd>β-catenin</kwd><kwd>autophagy</kwd><kwd>cordycepin</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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