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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effect of silencing S-phase kinase-associated protein 2 on chemosensitivity to temozolomide of human glioma cells U251</title><author><name sortKey="Liu, Yue" sort="Liu, Yue" uniqKey="Liu Y" first="Yue" last="Liu">Yue Liu</name></author><author><name sortKey="Tang, Zhen Gang" sort="Tang, Zhen Gang" uniqKey="Tang Z" first="Zhen-Gang" last="Tang">Zhen-Gang Tang</name></author><author><name sortKey="Yang, Jian Quan" sort="Yang, Jian Quan" uniqKey="Yang J" first="Jian-Quan" last="Yang">Jian-Quan Yang</name></author><author><name sortKey="Zhou, Yi" sort="Zhou, Yi" uniqKey="Zhou Y" first="Yi" last="Zhou">Yi Zhou</name></author><author><name sortKey="Lin, Yi" sort="Lin, Yi" uniqKey="Lin Y" first="Yi" last="Lin">Yi Lin</name></author><author><name sortKey="Lv, Wei" sort="Lv, Wei" uniqKey="Lv W" first="Wei" last="Lv">Wei Lv</name></author><author><name sortKey="Wang, Guo Bin" sort="Wang, Guo Bin" uniqKey="Wang G" first="Guo-Bin" last="Wang">Guo-Bin Wang</name></author><author><name sortKey="Li, Cai Li" sort="Li, Cai Li" uniqKey="Li C" first="Cai-Li" last="Li">Cai-Li Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31105854</idno><idno type="pmc">6511802</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511802</idno><idno type="RBID">PMC:6511802</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000351</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000351</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Effect of silencing S-phase kinase-associated protein 2 on chemosensitivity to temozolomide of human glioma cells U251</title><author><name sortKey="Liu, Yue" sort="Liu, Yue" uniqKey="Liu Y" first="Yue" last="Liu">Yue Liu</name></author><author><name sortKey="Tang, Zhen Gang" sort="Tang, Zhen Gang" uniqKey="Tang Z" first="Zhen-Gang" last="Tang">Zhen-Gang Tang</name></author><author><name sortKey="Yang, Jian Quan" sort="Yang, Jian Quan" uniqKey="Yang J" first="Jian-Quan" last="Yang">Jian-Quan Yang</name></author><author><name sortKey="Zhou, Yi" sort="Zhou, Yi" uniqKey="Zhou Y" first="Yi" last="Zhou">Yi Zhou</name></author><author><name sortKey="Lin, Yi" sort="Lin, Yi" uniqKey="Lin Y" first="Yi" last="Lin">Yi Lin</name></author><author><name sortKey="Lv, Wei" sort="Lv, Wei" uniqKey="Lv W" first="Wei" last="Lv">Wei Lv</name></author><author><name sortKey="Wang, Guo Bin" sort="Wang, Guo Bin" uniqKey="Wang G" first="Guo-Bin" last="Wang">Guo-Bin Wang</name></author><author><name sortKey="Li, Cai Li" sort="Li, Cai Li" uniqKey="Li C" first="Cai-Li" last="Li">Cai-Li Li</name></author></analytic><series><title level="j">American Journal of Translational Research</title><idno type="eISSN">1943-8141</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Objective: To examine the effect of silencing SKP2 on chemosensitivity of human glioma cells U251 to temozolomide (TMZ). Methods: Adenoviruses harbouring shRNA targeting SKP2 (i.e. Ad-shSKP2) and non-targeting scrambled shRNA (i.e. Ad-shNC) were used to infect U251 cells. The transduced cells were then treated with TMZ. Cell viability after treatment was assayed using CCK8; while cell cycle and apoptosis were examined using flow cytometry. To study the effect of silencing SKP2 on autophagy in U251, we co-transduced the cells with Ad-mRFP-LC3 and Ad-shSKP2/Ad-shNC. The expression of autophagy marker LC3 after TMZ treatment was studied using microscopy and Western blotting assays. Results: The cytotoxicity of TMZ (i.e. 20-100 µM) was more significantly seen in Ad-shSKP2-transduced U251 cells than in the Ad-shNC-transduced U251 cells. The IC<sub>50</sub> values in shSKP2-U251 were significantly lower than those of the shNC-U251 (<italic>P</italic> < 0.05). Both TMZ and Ad-shSKP2 alone increased apoptosis and promoted expression of LC3 in U251. Combined treatment of Ad-shSKP2 and TMZ further elevated apoptosis and LC3 expression. Conclusion: Silencing SKP2 in U251 cells increased chemosensitivity to TMZ that was accompanied with enhanced apoptosis and autophagy. Targeting SKP2 may be a potential approach to potentiate TMZ treatment in patients with glioma.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Am J Transl Res</journal-id><journal-id journal-id-type="iso-abbrev">Am J Transl Res</journal-id><journal-id journal-id-type="publisher-id">ajtr</journal-id><journal-title-group><journal-title>American Journal of Translational Research</journal-title></journal-title-group><issn pub-type="epub">1943-8141</issn><publisher><publisher-name>e-Century Publishing Corporation</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31105854</article-id><article-id pub-id-type="pmc">6511802</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Effect of silencing S-phase kinase-associated protein 2 on chemosensitivity to temozolomide of human glioma cells U251</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Yue</given-names></name></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Zhen-Gang</given-names></name></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Jian-Quan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Yi</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lin</surname><given-names>Yi</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lv</surname><given-names>Wei</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Guo-Bin</given-names></name></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Cai-Li</given-names></name></contrib><aff><institution>Department of Neurosurgery, Renmin Hospital, Hubei University of Medicine</institution><addr-line>Shiyan, Hubei, P.R. China</addr-line></aff></contrib-group><author-notes><corresp><bold>Address correspondence to:</bold> Cai-Li Li, Department of Neurosurgery, Renmin Hospital, Hubei University of Medicine, No. 39 Middle Chaoyang Road, Shiyan 442000, Hubei, P. R. China. Tel: +86-13597883520; E-mail: <email>licailili@163.com</email></corresp></author-notes><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>15</day><month>4</month><year>2019</year></pub-date><volume>11</volume><issue>4</issue><fpage>2470</fpage><lpage>2476</lpage><history><date date-type="received"><day>19</day><month>12</month><year>2018</year></date><date date-type="accepted"><day>16</day><month>1</month><year>2019</year></date></history><permissions><copyright-statement>AJTR Copyright © 2019</copyright-statement><copyright-year>2019</copyright-year></permissions><abstract><p>Objective: To examine the effect of silencing SKP2 on chemosensitivity of human glioma cells U251 to temozolomide (TMZ). Methods: Adenoviruses harbouring shRNA targeting SKP2 (i.e. Ad-shSKP2) and non-targeting scrambled shRNA (i.e. Ad-shNC) were used to infect U251 cells. The transduced cells were then treated with TMZ. Cell viability after treatment was assayed using CCK8; while cell cycle and apoptosis were examined using flow cytometry. To study the effect of silencing SKP2 on autophagy in U251, we co-transduced the cells with Ad-mRFP-LC3 and Ad-shSKP2/Ad-shNC. The expression of autophagy marker LC3 after TMZ treatment was studied using microscopy and Western blotting assays. Results: The cytotoxicity of TMZ (i.e. 20-100 µM) was more significantly seen in Ad-shSKP2-transduced U251 cells than in the Ad-shNC-transduced U251 cells. The IC<sub>50</sub> values in shSKP2-U251 were significantly lower than those of the shNC-U251 (<italic>P</italic> < 0.05). Both TMZ and Ad-shSKP2 alone increased apoptosis and promoted expression of LC3 in U251. Combined treatment of Ad-shSKP2 and TMZ further elevated apoptosis and LC3 expression. Conclusion: Silencing SKP2 in U251 cells increased chemosensitivity to TMZ that was accompanied with enhanced apoptosis and autophagy. Targeting SKP2 may be a potential approach to potentiate TMZ treatment in patients with glioma.</p></abstract><kwd-group><kwd>Glioma</kwd><kwd>S-phase kinase-associated protein 2 (SKP2)</kwd><kwd>temozolomide (TMZ)</kwd><kwd>chemosensitivity</kwd><kwd>autophagy</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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