Autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection
Identifieur interne : 000965 ( Pmc/Checkpoint ); précédent : 000964; suivant : 000966Autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection
Auteurs : Xiaosai Niu ; Chengcheng Zhang ; Yuyang Wang ; Mengjiao Guo ; Baoyang Ruan ; Xuefeng Wang ; Tianqi Wu ; Xiaorong Zhang ; Yantao WuSource :
- BMC Veterinary Research [ 1746-6148 ] ; 2019.
Abstract
Avian reovirus (ARV) is an important pathogen that can cause serious disease in poultry. Though several in vitro studies revealed some molecular mechanisms that are responsible for ARV-induced autophagy, it is still largely unknown how ARV manipulates autophagy to promote its own propagation.
In this study, we demonstrated that ARV infection triggered autophagy in chicken tissues, evident from the enhancement of LC3-I/−II conversion and the appearance of abundant autophagosomes. Moreover, viral replication and the expression of IL-1β were coupled with the process of ARV-induced autophagy in the early stage of infection. Furthermore, regulation of autophagy affected the accumulation of LC3-II, the production of ARV and the expression of IL-1β.
Altogether, our data suggest that ARV induces autophagy, which benefits its replication and dissemination in chicken tissues at the early infection stage.
The online version of this article (10.1186/s12917-019-1926-5) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12917-019-1926-5
PubMed: 31126305
PubMed Central: 6534907
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection</title><author><name sortKey="Niu, Xiaosai" sort="Niu, Xiaosai" uniqKey="Niu X" first="Xiaosai" last="Niu">Xiaosai Niu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Chengcheng" sort="Zhang, Chengcheng" uniqKey="Zhang C" first="Chengcheng" last="Zhang">Chengcheng Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Yuyang" sort="Wang, Yuyang" uniqKey="Wang Y" first="Yuyang" last="Wang">Yuyang Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Guo, Mengjiao" sort="Guo, Mengjiao" uniqKey="Guo M" first="Mengjiao" last="Guo">Mengjiao Guo</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Ruan, Baoyang" sort="Ruan, Baoyang" uniqKey="Ruan B" first="Baoyang" last="Ruan">Baoyang Ruan</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Xuefeng" sort="Wang, Xuefeng" uniqKey="Wang X" first="Xuefeng" last="Wang">Xuefeng Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Tianqi" sort="Wu, Tianqi" uniqKey="Wu T" first="Tianqi" last="Wu">Tianqi Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Xiaorong" sort="Zhang, Xiaorong" uniqKey="Zhang X" first="Xiaorong" last="Zhang">Xiaorong Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Yantao" sort="Wu, Yantao" uniqKey="Wu Y" first="Yantao" last="Wu">Yantao Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31126305</idno><idno type="pmc">6534907</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534907</idno><idno type="RBID">PMC:6534907</idno><idno type="doi">10.1186/s12917-019-1926-5</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000047</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000047</idno><idno type="wicri:Area/Pmc/Curation">000047</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000047</idno><idno type="wicri:Area/Pmc/Checkpoint">000965</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000965</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection</title><author><name sortKey="Niu, Xiaosai" sort="Niu, Xiaosai" uniqKey="Niu X" first="Xiaosai" last="Niu">Xiaosai Niu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Chengcheng" sort="Zhang, Chengcheng" uniqKey="Zhang C" first="Chengcheng" last="Zhang">Chengcheng Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Yuyang" sort="Wang, Yuyang" uniqKey="Wang Y" first="Yuyang" last="Wang">Yuyang Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Guo, Mengjiao" sort="Guo, Mengjiao" uniqKey="Guo M" first="Mengjiao" last="Guo">Mengjiao Guo</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Ruan, Baoyang" sort="Ruan, Baoyang" uniqKey="Ruan B" first="Baoyang" last="Ruan">Baoyang Ruan</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Xuefeng" sort="Wang, Xuefeng" uniqKey="Wang X" first="Xuefeng" last="Wang">Xuefeng Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Tianqi" sort="Wu, Tianqi" uniqKey="Wu T" first="Tianqi" last="Wu">Tianqi Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Xiaorong" sort="Zhang, Xiaorong" uniqKey="Zhang X" first="Xiaorong" last="Zhang">Xiaorong Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Yantao" sort="Wu, Yantao" uniqKey="Wu Y" first="Yantao" last="Wu">Yantao Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">BMC Veterinary Research</title><idno type="eISSN">1746-6148</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p id="Par1">Avian reovirus (ARV) is an important pathogen that can cause serious disease in poultry. Though several in vitro studies revealed some molecular mechanisms that are responsible for ARV-induced autophagy, it is still largely unknown how ARV manipulates autophagy to promote its own propagation.</p></sec><sec><title>Results</title><p id="Par2">In this study, we demonstrated that ARV infection triggered autophagy in chicken tissues, evident from the enhancement of LC3-I/−II conversion and the appearance of abundant autophagosomes. Moreover, viral replication and the expression of IL-1β were coupled with the process of ARV-induced autophagy in the early stage of infection. Furthermore, regulation of autophagy affected the accumulation of LC3-II, the production of ARV and the expression of IL-1β.</p></sec><sec><title>Conclusions</title><p id="Par3">Altogether, our data suggest that ARV induces autophagy, which benefits its replication and dissemination in chicken tissues at the early infection stage.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (10.1186/s12917-019-1926-5) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="King, Amq" uniqKey="King A">AMQ King</name></author><author><name sortKey="Adams, Mj" uniqKey="Adams M">MJ Adams</name></author><author><name sortKey="Carstens, Eb" uniqKey="Carstens E">EB Carstens</name></author><author><name sortKey="Lefkowitz, Ej" uniqKey="Lefkowitz E">EJ 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<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Vet Res</journal-id><journal-id journal-id-type="iso-abbrev">BMC Vet. Res</journal-id><journal-title-group><journal-title>BMC Veterinary Research</journal-title></journal-title-group><issn pub-type="epub">1746-6148</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31126305</article-id><article-id pub-id-type="pmc">6534907</article-id><article-id pub-id-type="publisher-id">1926</article-id><article-id pub-id-type="doi">10.1186/s12917-019-1926-5</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection</article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Niu</surname><given-names>Xiaosai</given-names></name><address><email>niusci@icloud.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Zhang</surname><given-names>Chengcheng</given-names></name><address><email>zcc@yzu.edu.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Yuyang</given-names></name><address><email>yywang@yzu.edu.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Guo</surname><given-names>Mengjiao</given-names></name><address><email>guomj@yzu.edu.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Ruan</surname><given-names>Baoyang</given-names></name><address><email>ruanbaoyang@sina.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Xuefeng</given-names></name><address><email>wxf901123@gmail.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Tianqi</given-names></name><address><email>2286270169@qq.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Xiaorong</given-names></name><address><email>zxr@yzu.edu.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-2647-395X</contrib-id><name><surname>Wu</surname><given-names>Yantao</given-names></name><address><phone>086-514-87991417</phone><email>ytwu@yzu.edu.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>Jiangsu Co-Innovation Center for Prevention of Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine,</institution><institution>Yangzhou University,</institution></institution-wrap>12 East Wenhui Road, Yangzhou, 225009 Jiangsu China</aff></contrib-group><pub-date pub-type="epub"><day>24</day><month>5</month><year>2019</year></pub-date><pub-date pub-type="pmc-release"><day>24</day><month>5</month><year>2019</year></pub-date><pub-date pub-type="collection"><year>2019</year></pub-date><volume>15</volume><elocation-id>173</elocation-id><history><date date-type="received"><day>31</day><month>1</month><year>2019</year></date><date date-type="accepted"><day>20</day><month>5</month><year>2019</year></date></history><permissions><copyright-statement>© The Author(s). 2019</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p id="Par1">Avian reovirus (ARV) is an important pathogen that can cause serious disease in poultry. Though several in vitro studies revealed some molecular mechanisms that are responsible for ARV-induced autophagy, it is still largely unknown how ARV manipulates autophagy to promote its own propagation.</p></sec><sec><title>Results</title><p id="Par2">In this study, we demonstrated that ARV infection triggered autophagy in chicken tissues, evident from the enhancement of LC3-I/−II conversion and the appearance of abundant autophagosomes. Moreover, viral replication and the expression of IL-1β were coupled with the process of ARV-induced autophagy in the early stage of infection. Furthermore, regulation of autophagy affected the accumulation of LC3-II, the production of ARV and the expression of IL-1β.</p></sec><sec><title>Conclusions</title><p id="Par3">Altogether, our data suggest that ARV induces autophagy, which benefits its replication and dissemination in chicken tissues at the early infection stage.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (10.1186/s12917-019-1926-5) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Avian reovirus</kwd><kwd>Autophagy</kwd><kwd>IL-1β</kwd><kwd>Chicken tissues</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100001809</institution-id><institution>National Natural Science Foundation of China</institution></institution-wrap></funding-source><award-id>31272576</award-id><principal-award-recipient><name><surname>Wu</surname><given-names>Yantao</given-names></name></principal-award-recipient></award-group></funding-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100010203</institution-id><institution>Agriculture Research System of China</institution></institution-wrap></funding-source><award-id>CARS-40-K16</award-id><principal-award-recipient><name><surname>Wu</surname><given-names>Yantao</given-names></name></principal-award-recipient></award-group></funding-group><funding-group><award-group><funding-source><institution>‘High-end talent support program’ of Yangzhou University</institution></funding-source><award-id>0</award-id><principal-award-recipient><name><surname>Wu</surname><given-names>Yantao</given-names></name></principal-award-recipient></award-group></funding-group><funding-group><award-group><funding-source><institution>the Priority Academic Program Development of Jiangsu Higher Education Institutions</institution></funding-source><award-id>0</award-id><principal-award-recipient><name><surname>Wu</surname><given-names>Yantao</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2019</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Guo, Mengjiao" sort="Guo, Mengjiao" uniqKey="Guo M" first="Mengjiao" last="Guo">Mengjiao Guo</name><name sortKey="Niu, Xiaosai" sort="Niu, Xiaosai" uniqKey="Niu X" first="Xiaosai" last="Niu">Xiaosai Niu</name><name sortKey="Ruan, Baoyang" sort="Ruan, Baoyang" uniqKey="Ruan B" first="Baoyang" last="Ruan">Baoyang Ruan</name><name sortKey="Wang, Xuefeng" sort="Wang, Xuefeng" uniqKey="Wang X" first="Xuefeng" last="Wang">Xuefeng Wang</name><name sortKey="Wang, Yuyang" sort="Wang, Yuyang" uniqKey="Wang Y" first="Yuyang" last="Wang">Yuyang Wang</name><name sortKey="Wu, Tianqi" sort="Wu, Tianqi" uniqKey="Wu T" first="Tianqi" last="Wu">Tianqi Wu</name><name sortKey="Wu, Yantao" sort="Wu, Yantao" uniqKey="Wu Y" first="Yantao" last="Wu">Yantao Wu</name><name sortKey="Zhang, Chengcheng" sort="Zhang, Chengcheng" uniqKey="Zhang C" first="Chengcheng" last="Zhang">Chengcheng Zhang</name><name sortKey="Zhang, Xiaorong" sort="Zhang, Xiaorong" uniqKey="Zhang X" first="Xiaorong" last="Zhang">Xiaorong Zhang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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