Bitter Taste Receptors (TAS2Rs) in Human Lung Macrophages: Receptor Expression and Inhibitory Effects of TAS2R Agonists
Identifieur interne : 000933 ( Pmc/Checkpoint ); précédent : 000932; suivant : 000934Bitter Taste Receptors (TAS2Rs) in Human Lung Macrophages: Receptor Expression and Inhibitory Effects of TAS2R Agonists
Auteurs : Stanislas Grassin-Delyle [France] ; Hélène Salvator [France] ; Nikola Mantov [France] ; Charlotte Abrial [France] ; Marion Brollo [France] ; Christophe Faisy [France] ; Emmanuel Naline [France] ; Louis-Jean Couderc [France] ; Philippe Devillier [France]Source :
- Frontiers in Physiology [ 1664-042X ] ; 2019.
Abstract
Bitter-taste receptors (TAS2Rs) are involved in airway relaxation but are also expressed in human blood leukocytes. We studied TAS2R expression and the effects of TAS2R agonists on the lipopolysaccharide (LPS)-induced cytokine release in human lung macrophages (LMs).
Lung macrophages were isolated from patients undergoing surgery for carcinoma. We used RT-qPCR to measure transcripts of 16 TAS2Rs (TAS2Rs 3/4/5/7/8/9/10/14/19/20/31/38/39/43/45 and 46) in unstimulated and LPS-stimulated (10 ng.mL–1) LMs. The macrophages were also incubated with TAS2R agonists for 24 h. Supernatant levels of the cytokines TNF-α, CCL3, CXCL8 and IL-10 were measured using ELISAs.
The transcripts of all 16 TAS2Rs were detected in macrophages. The addition of LPS led to an increase in the expression of most TAS2Rs, which was significant for TAS2R7 and 38. Although the promiscuous TAS2R agonists, quinine and denatonium, inhibited the LPS-induced release of TNF-α, CCL3 and CXCL8, diphenidol was inactive. Partially selective agonists (dapsone, colchicine, strychnine, and chloroquine) and selective agonists [erythromycin (TAS2R10), phenanthroline (TAS2R5), ofloxacin (TAS2R9), and carisoprodol (TAS2R14)] also suppressed the LPS-induced cytokine release. In contrast, two other agonists [sodium cromoglycate (TAS2R20) and saccharin (TAS2R31 and 43)] were inactive. TAS2R agonists suppressed IL-10 production – suggesting that this anti-inflammatory cytokine is not involved in the inhibition of cytokine production.
Human LMs expressed TAS2Rs. Experiments with TAS2R agonists’ suggested the involvement of TAS2Rs 3, 4, 5, 9, 10, 14, 30, 39 and 40 in the inhibition of cytokine production. TAS2Rs may constitute new drug targets in inflammatory obstructive lung disease.
Url:
DOI: 10.3389/fphys.2019.01267
PubMed: 31632299
PubMed Central: 6783802
Affiliations:
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<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Couderc, Louis Jean" sort="Couderc, Louis Jean" uniqKey="Couderc L" first="Louis-Jean" last="Couderc">Louis-Jean Couderc</name>
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,<addr-line>Suresnes</addr-line>
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<country xml:lang="fr">France</country>
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<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Devillier, Philippe" sort="Devillier, Philippe" uniqKey="Devillier P" first="Philippe" last="Devillier">Philippe Devillier</name>
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,<addr-line>Suresnes</addr-line>
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</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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,<addr-line>Suresnes</addr-line>
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<country xml:lang="fr">France</country>
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<series><title level="j">Frontiers in Physiology</title>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Bitter-taste receptors (TAS2Rs) are involved in airway relaxation but are also expressed in human blood leukocytes. We studied TAS2R expression and the effects of TAS2R agonists on the lipopolysaccharide (LPS)-induced cytokine release in human lung macrophages (LMs).</p>
</sec>
<sec><title>Methods</title>
<p>Lung macrophages were isolated from patients undergoing surgery for carcinoma. We used RT-qPCR to measure transcripts of 16 TAS2Rs (TAS2Rs 3/4/5/7/8/9/10/14/19/20/31/38/39/43/45 and 46) in unstimulated and LPS-stimulated (10 ng.mL<sup>–1</sup>
) LMs. The macrophages were also incubated with TAS2R agonists for 24 h. Supernatant levels of the cytokines TNF-α, CCL3, CXCL8 and IL-10 were measured using ELISAs.</p>
</sec>
<sec><title>Results</title>
<p>The transcripts of all 16 TAS2Rs were detected in macrophages. The addition of LPS led to an increase in the expression of most TAS2Rs, which was significant for TAS2R7 and 38. Although the promiscuous TAS2R agonists, quinine and denatonium, inhibited the LPS-induced release of TNF-α, CCL3 and CXCL8, diphenidol was inactive. Partially selective agonists (dapsone, colchicine, strychnine, and chloroquine) and selective agonists [erythromycin (TAS2R10), phenanthroline (TAS2R5), ofloxacin (TAS2R9), and carisoprodol (TAS2R14)] also suppressed the LPS-induced cytokine release. In contrast, two other agonists [sodium cromoglycate (TAS2R20) and saccharin (TAS2R31 and 43)] were inactive. TAS2R agonists suppressed IL-10 production – suggesting that this anti-inflammatory cytokine is not involved in the inhibition of cytokine production.</p>
</sec>
<sec><title>Conclusion</title>
<p>Human LMs expressed TAS2Rs. Experiments with TAS2R agonists’ suggested the involvement of TAS2Rs 3, 4, 5, 9, 10, 14, 30, 39 and 40 in the inhibition of cytokine production. TAS2Rs may constitute new drug targets in inflammatory obstructive lung disease.</p>
</sec>
</div>
</front>
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<author><name sortKey="Brollo, M" uniqKey="Brollo M">M. Brollo</name>
</author>
<author><name sortKey="Naline, E" uniqKey="Naline E">E. Naline</name>
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<author><name sortKey="Devillier, P" uniqKey="Devillier P">P. Devillier</name>
</author>
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<biblStruct><analytic><author><name sortKey="Alexander, S P H" uniqKey="Alexander S">S. P. H. Alexander</name>
</author>
<author><name sortKey="Mathie, A" uniqKey="Mathie A">A. Mathie</name>
</author>
<author><name sortKey="Peters, J A" uniqKey="Peters J">J. A. Peters</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Allen, J N" uniqKey="Allen J">J. N. Allen</name>
</author>
<author><name sortKey="Herzyk, D J" uniqKey="Herzyk D">D. J. Herzyk</name>
</author>
<author><name sortKey="Wewers, M D" uniqKey="Wewers M">M. D. Wewers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Allen, J N" uniqKey="Allen J">J. N. Allen</name>
</author>
<author><name sortKey="Moore, S A" uniqKey="Moore S">S. A. Moore</name>
</author>
<author><name sortKey="Liao, Z" uniqKey="Liao Z">Z. Liao</name>
</author>
<author><name sortKey="Wewers, M D" uniqKey="Wewers M">M. D. Wewers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="An, S S" uniqKey="An S">S. S. An</name>
</author>
<author><name sortKey="Wang, W C" uniqKey="Wang W">W. C. Wang</name>
</author>
<author><name sortKey="Koziol White, C J" uniqKey="Koziol White C">C. J. Koziol-White</name>
</author>
<author><name sortKey="Ahn, K" uniqKey="Ahn K">K. Ahn</name>
</author>
<author><name sortKey="Lee, D Y" uniqKey="Lee D">D. Y. Lee</name>
</author>
<author><name sortKey="Kurten, R C" uniqKey="Kurten R">R. C. Kurten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Andres Barquin, P J" uniqKey="Andres Barquin P">P. J. Andres-Barquin</name>
</author>
<author><name sortKey="Conte, C" uniqKey="Conte C">C. Conte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Armstrong, J" uniqKey="Armstrong J">J. Armstrong</name>
</author>
<author><name sortKey="Harbron, C" uniqKey="Harbron C">C. Harbron</name>
</author>
<author><name sortKey="Lea, S" uniqKey="Lea S">S. Lea</name>
</author>
<author><name sortKey="Booth, G" uniqKey="Booth G">G. Booth</name>
</author>
<author><name sortKey="Cadden, P" uniqKey="Cadden P">P. Cadden</name>
</author>
<author><name sortKey="Wreggett, K A" uniqKey="Wreggett K">K. A. Wreggett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Armstrong, J" uniqKey="Armstrong J">J. Armstrong</name>
</author>
<author><name sortKey="Sargent, C" uniqKey="Sargent C">C. Sargent</name>
</author>
<author><name sortKey="Singh, D" uniqKey="Singh D">D. Singh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Armstrong, L" uniqKey="Armstrong L">L. Armstrong</name>
</author>
<author><name sortKey="Jordan, N" uniqKey="Jordan N">N. Jordan</name>
</author>
<author><name sortKey="Millar, A" uniqKey="Millar A">A. Millar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Balhara, J" uniqKey="Balhara J">J. Balhara</name>
</author>
<author><name sortKey="Gounni, A S" uniqKey="Gounni A">A. S. Gounni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barnes, P J" uniqKey="Barnes P">P. J. Barnes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Behrens, M" uniqKey="Behrens M">M. Behrens</name>
</author>
<author><name sortKey="Meyerhof, W" uniqKey="Meyerhof W">W. Meyerhof</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Berkman, N" uniqKey="Berkman N">N. Berkman</name>
</author>
<author><name sortKey="John, M" uniqKey="John M">M. John</name>
</author>
<author><name sortKey="Roesems, G" uniqKey="Roesems G">G. Roesems</name>
</author>
<author><name sortKey="Jose, P J" uniqKey="Jose P">P. J. Jose</name>
</author>
<author><name sortKey="Barnes, P J" uniqKey="Barnes P">P. J. Barnes</name>
</author>
<author><name sortKey="Chung, K F" uniqKey="Chung K">K. F. Chung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buenestado, A" uniqKey="Buenestado A">A. Buenestado</name>
</author>
<author><name sortKey="Grassin Delyle, S" uniqKey="Grassin Delyle S">S. Grassin Delyle</name>
</author>
<author><name sortKey="Arnould, I" uniqKey="Arnould I">I. Arnould</name>
</author>
<author><name sortKey="Besnard, F" uniqKey="Besnard F">F. Besnard</name>
</author>
<author><name sortKey="Naline, E" uniqKey="Naline E">E. Naline</name>
</author>
<author><name sortKey="Blouquit Laye, S" uniqKey="Blouquit Laye S">S. Blouquit-Laye</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buenestado, A" uniqKey="Buenestado A">A. Buenestado</name>
</author>
<author><name sortKey="Grassin Delyle, S" uniqKey="Grassin Delyle S">S. Grassin-Delyle</name>
</author>
<author><name sortKey="Guitard, F" uniqKey="Guitard F">F. Guitard</name>
</author>
<author><name sortKey="Naline, E" uniqKey="Naline E">E. Naline</name>
</author>
<author><name sortKey="Faisy, C" uniqKey="Faisy C">C. Faisy</name>
</author>
<author><name sortKey="Israel Biet, D" uniqKey="Israel Biet D">D. Israel-Biet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cai, M" uniqKey="Cai M">M. Cai</name>
</author>
<author><name sortKey="Bonella, F" uniqKey="Bonella F">F. Bonella</name>
</author>
<author><name sortKey="Dai, H" uniqKey="Dai H">H. Dai</name>
</author>
<author><name sortKey="Sarria, R" uniqKey="Sarria R">R. Sarria</name>
</author>
<author><name sortKey="Guzman, J" uniqKey="Guzman J">J. Guzman</name>
</author>
<author><name sortKey="Costabel, U" uniqKey="Costabel U">U. Costabel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Charous, B L" uniqKey="Charous B">B. L. Charous</name>
</author>
<author><name sortKey="Halpern, E F" uniqKey="Halpern E">E. F. Halpern</name>
</author>
<author><name sortKey="Steven, G C" uniqKey="Steven G">G. C. Steven</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name>
</author>
<author><name sortKey="Cowan, M J" uniqKey="Cowan M">M. J. Cowan</name>
</author>
<author><name sortKey="Hasday, J D" uniqKey="Hasday J">J. D. Hasday</name>
</author>
<author><name sortKey="Vogel, S N" uniqKey="Vogel S">S. N. Vogel</name>
</author>
<author><name sortKey="Medvedev, A E" uniqKey="Medvedev A">A. E. Medvedev</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cohen, N A" uniqKey="Cohen N">N. A. Cohen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Deshpande, D A" uniqKey="Deshpande D">D. A. Deshpande</name>
</author>
<author><name sortKey="Wang, W C" uniqKey="Wang W">W. C. Wang</name>
</author>
<author><name sortKey="Mcilmoyle, E L" uniqKey="Mcilmoyle E">E. L. McIlmoyle</name>
</author>
<author><name sortKey="Robinett, K S" uniqKey="Robinett K">K. S. Robinett</name>
</author>
<author><name sortKey="Schillinger, R M" uniqKey="Schillinger R">R. M. Schillinger</name>
</author>
<author><name sortKey="An, S S" uniqKey="An S">S. S. An</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Di Pizio, A" uniqKey="Di Pizio A">A. Di Pizio</name>
</author>
<author><name sortKey="Niv, M Y" uniqKey="Niv M">M. Y. Niv</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dotson, C D" uniqKey="Dotson C">C. D. Dotson</name>
</author>
<author><name sortKey="Zhang, L" uniqKey="Zhang L">L. Zhang</name>
</author>
<author><name sortKey="Xu, H" uniqKey="Xu H">H. Xu</name>
</author>
<author><name sortKey="Shin, Y K" uniqKey="Shin Y">Y. K. Shin</name>
</author>
<author><name sortKey="Vigues, S" uniqKey="Vigues S">S. Vigues</name>
</author>
<author><name sortKey="Ott, S H" uniqKey="Ott S">S. H. Ott</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ekoff, M" uniqKey="Ekoff M">M. Ekoff</name>
</author>
<author><name sortKey="Choi, J H" uniqKey="Choi J">J. H. Choi</name>
</author>
<author><name sortKey="James, A" uniqKey="James A">A. James</name>
</author>
<author><name sortKey="Dahlen, B" uniqKey="Dahlen B">B. Dahlen</name>
</author>
<author><name sortKey="Nilsson, G" uniqKey="Nilsson G">G. Nilsson</name>
</author>
<author><name sortKey="Dahlen, S E" uniqKey="Dahlen S">S. E. Dahlen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gaida, M M" uniqKey="Gaida M">M. M. Gaida</name>
</author>
<author><name sortKey="Dapunt, U" uniqKey="Dapunt U">U. Dapunt</name>
</author>
<author><name sortKey="Hansch, G M" uniqKey="Hansch G">G. M. Hansch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grassin Delyle, S" uniqKey="Grassin Delyle S">S. Grassin-Delyle</name>
</author>
<author><name sortKey="Abrial, C" uniqKey="Abrial C">C. Abrial</name>
</author>
<author><name sortKey="Fayad Kobeissi, S" uniqKey="Fayad Kobeissi S">S. Fayad-Kobeissi</name>
</author>
<author><name sortKey="Brollo, M" uniqKey="Brollo M">M. Brollo</name>
</author>
<author><name sortKey="Faisy, C" uniqKey="Faisy C">C. Faisy</name>
</author>
<author><name sortKey="Alvarez, J C" uniqKey="Alvarez J">J. C. Alvarez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grassin Delyle, S" uniqKey="Grassin Delyle S">S. Grassin-Delyle</name>
</author>
<author><name sortKey="Abrial, C" uniqKey="Abrial C">C. Abrial</name>
</author>
<author><name sortKey="Salvator, H" uniqKey="Salvator H">H. Salvator</name>
</author>
<author><name sortKey="Brollo, M" uniqKey="Brollo M">M. Brollo</name>
</author>
<author><name sortKey="Naline, E" uniqKey="Naline E">E. Naline</name>
</author>
<author><name sortKey="Devillier, P" uniqKey="Devillier P">P. Devillier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Groot Kormelink, P J" uniqKey="Groot Kormelink P">P. J. Groot-Kormelink</name>
</author>
<author><name sortKey="Fawcett, L" uniqKey="Fawcett L">L. Fawcett</name>
</author>
<author><name sortKey="Wright, P D" uniqKey="Wright P">P. D. Wright</name>
</author>
<author><name sortKey="Gosling, M" uniqKey="Gosling M">M. Gosling</name>
</author>
<author><name sortKey="Kent, T C" uniqKey="Kent T">T. C. Kent</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Higham, A" uniqKey="Higham A">A. Higham</name>
</author>
<author><name sortKey="Booth, G" uniqKey="Booth G">G. Booth</name>
</author>
<author><name sortKey="Lea, S" uniqKey="Lea S">S. Lea</name>
</author>
<author><name sortKey="Southworth, T" uniqKey="Southworth T">T. Southworth</name>
</author>
<author><name sortKey="Plumb, J" uniqKey="Plumb J">J. Plumb</name>
</author>
<author><name sortKey="Singh, D" uniqKey="Singh D">D. Singh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Higham, A" uniqKey="Higham A">A. Higham</name>
</author>
<author><name sortKey="Lea, S" uniqKey="Lea S">S. Lea</name>
</author>
<author><name sortKey="Ray, D" uniqKey="Ray D">D. Ray</name>
</author>
<author><name sortKey="Singh, D" uniqKey="Singh D">D. Singh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hodge, S" uniqKey="Hodge S">S. Hodge</name>
</author>
<author><name sortKey="Matthews, G" uniqKey="Matthews G">G. Matthews</name>
</author>
<author><name sortKey="Mukaro, V" uniqKey="Mukaro V">V. Mukaro</name>
</author>
<author><name sortKey="Ahern, J" uniqKey="Ahern J">J. Ahern</name>
</author>
<author><name sortKey="Shivam, A" uniqKey="Shivam A">A. Shivam</name>
</author>
<author><name sortKey="Hodge, G" uniqKey="Hodge G">G. Hodge</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ianaro, A" uniqKey="Ianaro A">A. Ianaro</name>
</author>
<author><name sortKey="Ialenti, A" uniqKey="Ialenti A">A. Ialenti</name>
</author>
<author><name sortKey="Maffia, P" uniqKey="Maffia P">P. Maffia</name>
</author>
<author><name sortKey="Sautebin, L" uniqKey="Sautebin L">L. Sautebin</name>
</author>
<author><name sortKey="Rombola, L" uniqKey="Rombola L">L. Rombola</name>
</author>
<author><name sortKey="Carnuccio, R" uniqKey="Carnuccio R">R. Carnuccio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jaggupilli, A" uniqKey="Jaggupilli A">A. Jaggupilli</name>
</author>
<author><name sortKey="Singh, N" uniqKey="Singh N">N. Singh</name>
</author>
<author><name sortKey="De Jesus, V C" uniqKey="De Jesus V">V. C. De Jesus</name>
</author>
<author><name sortKey="Gounni, M S" uniqKey="Gounni M">M. S. Gounni</name>
</author>
<author><name sortKey="Dhanaraj, P" uniqKey="Dhanaraj P">P. Dhanaraj</name>
</author>
<author><name sortKey="Chelikani, P" uniqKey="Chelikani P">P. Chelikani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jaggupilli, A" uniqKey="Jaggupilli A">A. Jaggupilli</name>
</author>
<author><name sortKey="Singh, N" uniqKey="Singh N">N. Singh</name>
</author>
<author><name sortKey="Upadhyaya, J" uniqKey="Upadhyaya J">J. Upadhyaya</name>
</author>
<author><name sortKey="Sikarwar, A S" uniqKey="Sikarwar A">A. S. Sikarwar</name>
</author>
<author><name sortKey="Arakawa, M" uniqKey="Arakawa M">M. Arakawa</name>
</author>
<author><name sortKey="Dakshinamurti, S" uniqKey="Dakshinamurti S">S. Dakshinamurti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jang, C H" uniqKey="Jang C">C. H. Jang</name>
</author>
<author><name sortKey="Choi, J H" uniqKey="Choi J">J. H. Choi</name>
</author>
<author><name sortKey="Byun, M S" uniqKey="Byun M">M. S. Byun</name>
</author>
<author><name sortKey="Jue, D M" uniqKey="Jue D">D. M. Jue</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jeong, J Y" uniqKey="Jeong J">J. Y. Jeong</name>
</author>
<author><name sortKey="Jue, D M" uniqKey="Jue D">D. M. Jue</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jeyaseelan, S" uniqKey="Jeyaseelan S">S. Jeyaseelan</name>
</author>
<author><name sortKey="Manzer, R" uniqKey="Manzer R">R. Manzer</name>
</author>
<author><name sortKey="Young, S K" uniqKey="Young S">S. K. Young</name>
</author>
<author><name sortKey="Yamamoto, M" uniqKey="Yamamoto M">M. Yamamoto</name>
</author>
<author><name sortKey="Akira, S" uniqKey="Akira S">S. Akira</name>
</author>
<author><name sortKey="Mason, R J" uniqKey="Mason R">R. J. Mason</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jin, M" uniqKey="Jin M">M. Jin</name>
</author>
<author><name sortKey="Opalek, J M" uniqKey="Opalek J">J. M. Opalek</name>
</author>
<author><name sortKey="Marsh, C B" uniqKey="Marsh C">C. B. Marsh</name>
</author>
<author><name sortKey="Wu, H M" uniqKey="Wu H">H. M. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, R J" uniqKey="Lee R">R. J. Lee</name>
</author>
<author><name sortKey="Xiong, G" uniqKey="Xiong G">G. Xiong</name>
</author>
<author><name sortKey="Kofonow, J M" uniqKey="Kofonow J">J. M. Kofonow</name>
</author>
<author><name sortKey="Chen, B" uniqKey="Chen B">B. Chen</name>
</author>
<author><name sortKey="Lysenko, A" uniqKey="Lysenko A">A. Lysenko</name>
</author>
<author><name sortKey="Jiang, P" uniqKey="Jiang P">P. Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name>
</author>
<author><name sortKey="Pritchard, D K" uniqKey="Pritchard D">D. K. Pritchard</name>
</author>
<author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name>
</author>
<author><name sortKey="Park, D R" uniqKey="Park D">D. R. Park</name>
</author>
<author><name sortKey="Bumgarner, R E" uniqKey="Bumgarner R">R. E. Bumgarner</name>
</author>
<author><name sortKey="Schwartz, S M" uniqKey="Schwartz S">S. M. Schwartz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, K" uniqKey="Liu K">K. Liu</name>
</author>
<author><name sortKey="Jaggupilli, A" uniqKey="Jaggupilli A">A. Jaggupilli</name>
</author>
<author><name sortKey="Premnath, D" uniqKey="Premnath D">D. Premnath</name>
</author>
<author><name sortKey="Chelikani, P" uniqKey="Chelikani P">P. Chelikani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Malki, A" uniqKey="Malki A">A. Malki</name>
</author>
<author><name sortKey="Fiedler, J" uniqKey="Fiedler J">J. Fiedler</name>
</author>
<author><name sortKey="Fricke, K" uniqKey="Fricke K">K. Fricke</name>
</author>
<author><name sortKey="Ballweg, I" uniqKey="Ballweg I">I. Ballweg</name>
</author>
<author><name sortKey="Pfaffl, M W" uniqKey="Pfaffl M">M. W. Pfaffl</name>
</author>
<author><name sortKey="Krautwurst, D" uniqKey="Krautwurst D">D. Krautwurst</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maurer, S" uniqKey="Maurer S">S. Maurer</name>
</author>
<author><name sortKey="Wabnitz, G H" uniqKey="Wabnitz G">G. H. Wabnitz</name>
</author>
<author><name sortKey="Kahle, N A" uniqKey="Kahle N">N. A. Kahle</name>
</author>
<author><name sortKey="Stegmaier, S" uniqKey="Stegmaier S">S. Stegmaier</name>
</author>
<author><name sortKey="Prior, B" uniqKey="Prior B">B. Prior</name>
</author>
<author><name sortKey="Giese, T" uniqKey="Giese T">T. Giese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyerhof, W" uniqKey="Meyerhof W">W. Meyerhof</name>
</author>
<author><name sortKey="Batram, C" uniqKey="Batram C">C. Batram</name>
</author>
<author><name sortKey="Kuhn, C" uniqKey="Kuhn C">C. Kuhn</name>
</author>
<author><name sortKey="Brockhoff, A" uniqKey="Brockhoff A">A. Brockhoff</name>
</author>
<author><name sortKey="Chudoba, E" uniqKey="Chudoba E">E. Chudoba</name>
</author>
<author><name sortKey="Bufe, B" uniqKey="Bufe B">B. Bufe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Munic, V" uniqKey="Munic V">V. Munic</name>
</author>
<author><name sortKey="Banjanac, M" uniqKey="Banjanac M">M. Banjanac</name>
</author>
<author><name sortKey="Kostrun, S" uniqKey="Kostrun S">S. Kostrun</name>
</author>
<author><name sortKey="Nujic, K" uniqKey="Nujic K">K. Nujic</name>
</author>
<author><name sortKey="Bosnar, M" uniqKey="Bosnar M">M. Bosnar</name>
</author>
<author><name sortKey="Marjanovic, N" uniqKey="Marjanovic N">N. Marjanovic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murphy, B S" uniqKey="Murphy B">B. S. Murphy</name>
</author>
<author><name sortKey="Sundareshan, V" uniqKey="Sundareshan V">V. Sundareshan</name>
</author>
<author><name sortKey="Cory, T J" uniqKey="Cory T">T. J. Cory</name>
</author>
<author><name sortKey="Hayes, D" uniqKey="Hayes D">D. Hayes</name>
</author>
<author><name sortKey="Anstead, M I" uniqKey="Anstead M">M. I. Anstead</name>
</author>
<author><name sortKey="Feola, D J" uniqKey="Feola D">D. J. Feola</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nujic, K" uniqKey="Nujic K">K. Nujic</name>
</author>
<author><name sortKey="Banjanac, M" uniqKey="Banjanac M">M. Banjanac</name>
</author>
<author><name sortKey="Munic, V" uniqKey="Munic V">V. Munic</name>
</author>
<author><name sortKey="Polancec, D" uniqKey="Polancec D">D. Polancec</name>
</author>
<author><name sortKey="Erakovic Haber, V" uniqKey="Erakovic Haber V">V. Erakovic Haber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ogino, H" uniqKey="Ogino H">H. Ogino</name>
</author>
<author><name sortKey="Fujii, M" uniqKey="Fujii M">M. Fujii</name>
</author>
<author><name sortKey="Ono, M" uniqKey="Ono M">M. Ono</name>
</author>
<author><name sortKey="Maezawa, K" uniqKey="Maezawa K">K. Maezawa</name>
</author>
<author><name sortKey="Hori, S" uniqKey="Hori S">S. Hori</name>
</author>
<author><name sortKey="Kizu, J" uniqKey="Kizu J">J. Kizu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Orsmark Pietras, C" uniqKey="Orsmark Pietras C">C. Orsmark-Pietras</name>
</author>
<author><name sortKey="James, A" uniqKey="James A">A. James</name>
</author>
<author><name sortKey="Konradsen, J R" uniqKey="Konradsen J">J. R. Konradsen</name>
</author>
<author><name sortKey="Nordlund, B" uniqKey="Nordlund B">B. Nordlund</name>
</author>
<author><name sortKey="Soderhall, C" uniqKey="Soderhall C">C. Soderhall</name>
</author>
<author><name sortKey="Pulkkinen, V" uniqKey="Pulkkinen V">V. Pulkkinen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Plumb, J" uniqKey="Plumb J">J. Plumb</name>
</author>
<author><name sortKey="Robinson, L" uniqKey="Robinson L">L. Robinson</name>
</author>
<author><name sortKey="Lea, S" uniqKey="Lea S">S. Lea</name>
</author>
<author><name sortKey="Banyard, A" uniqKey="Banyard A">A. Banyard</name>
</author>
<author><name sortKey="Blaikley, J" uniqKey="Blaikley J">J. Blaikley</name>
</author>
<author><name sortKey="Ray, D" uniqKey="Ray D">D. Ray</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roland, W S" uniqKey="Roland W">W. S. Roland</name>
</author>
<author><name sortKey="Gouka, R J" uniqKey="Gouka R">R. J. Gouka</name>
</author>
<author><name sortKey="Gruppen, H" uniqKey="Gruppen H">H. Gruppen</name>
</author>
<author><name sortKey="Driesse, M" uniqKey="Driesse M">M. Driesse</name>
</author>
<author><name sortKey="Van Buren, L" uniqKey="Van Buren L">L. van Buren</name>
</author>
<author><name sortKey="Smit, G" uniqKey="Smit G">G. Smit</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sainz, E" uniqKey="Sainz E">E. Sainz</name>
</author>
<author><name sortKey="Cavenagh, M M" uniqKey="Cavenagh M">M. M. Cavenagh</name>
</author>
<author><name sortKey="Gutierrez, J" uniqKey="Gutierrez J">J. Gutierrez</name>
</author>
<author><name sortKey="Battey, J F" uniqKey="Battey J">J. F. Battey</name>
</author>
<author><name sortKey="Northup, J K" uniqKey="Northup J">J. K. Northup</name>
</author>
<author><name sortKey="Sullivan, S L" uniqKey="Sullivan S">S. L. Sullivan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schierbeck, H" uniqKey="Schierbeck H">H. Schierbeck</name>
</author>
<author><name sortKey="Wahamaa, H" uniqKey="Wahamaa H">H. Wahamaa</name>
</author>
<author><name sortKey="Andersson, U" uniqKey="Andersson U">U. Andersson</name>
</author>
<author><name sortKey="Harris, H E" uniqKey="Harris H">H. E. Harris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shah, A S" uniqKey="Shah A">A. S. Shah</name>
</author>
<author><name sortKey="Ben Shahar, Y" uniqKey="Ben Shahar Y">Y. Ben-Shahar</name>
</author>
<author><name sortKey="Moninger, T O" uniqKey="Moninger T">T. O. Moninger</name>
</author>
<author><name sortKey="Kline, J N" uniqKey="Kline J">J. N. Kline</name>
</author>
<author><name sortKey="Welsh, M J" uniqKey="Welsh M">M. J. Welsh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shaykhiev, R" uniqKey="Shaykhiev R">R. Shaykhiev</name>
</author>
<author><name sortKey="Krause, A" uniqKey="Krause A">A. Krause</name>
</author>
<author><name sortKey="Salit, J" uniqKey="Salit J">J. Salit</name>
</author>
<author><name sortKey="Strulovici Barel, Y" uniqKey="Strulovici Barel Y">Y. Strulovici-Barel</name>
</author>
<author><name sortKey="Harvey, B G" uniqKey="Harvey B">B. G. Harvey</name>
</author>
<author><name sortKey="O Onnor, T P" uniqKey="O Onnor T">T. P. O’Connor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Soares, S" uniqKey="Soares S">S. Soares</name>
</author>
<author><name sortKey="Kohl, S" uniqKey="Kohl S">S. Kohl</name>
</author>
<author><name sortKey="Thalmann, S" uniqKey="Thalmann S">S. Thalmann</name>
</author>
<author><name sortKey="Mateus, N" uniqKey="Mateus N">N. Mateus</name>
</author>
<author><name sortKey="Meyerhof, W" uniqKey="Meyerhof W">W. Meyerhof</name>
</author>
<author><name sortKey="De Freitas, V" uniqKey="De Freitas V">V. De Freitas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sugiyama, K" uniqKey="Sugiyama K">K. Sugiyama</name>
</author>
<author><name sortKey="Shirai, R" uniqKey="Shirai R">R. Shirai</name>
</author>
<author><name sortKey="Mukae, H" uniqKey="Mukae H">H. Mukae</name>
</author>
<author><name sortKey="Ishimoto, H" uniqKey="Ishimoto H">H. Ishimoto</name>
</author>
<author><name sortKey="Nagata, T" uniqKey="Nagata T">T. Nagata</name>
</author>
<author><name sortKey="Sakamoto, N" uniqKey="Sakamoto N">N. Sakamoto</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tomechko, S E" uniqKey="Tomechko S">S. E. Tomechko</name>
</author>
<author><name sortKey="Lundberg, K C" uniqKey="Lundberg K">K. C. Lundberg</name>
</author>
<author><name sortKey="Jarvela, J" uniqKey="Jarvela J">J. Jarvela</name>
</author>
<author><name sortKey="Bebek, G" uniqKey="Bebek G">G. Bebek</name>
</author>
<author><name sortKey="Chesnokov, N G" uniqKey="Chesnokov N">N. G. Chesnokov</name>
</author>
<author><name sortKey="Schlatzer, D" uniqKey="Schlatzer D">D. Schlatzer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tran, H T T" uniqKey="Tran H">H. T. T. Tran</name>
</author>
<author><name sortKey="Herz, C" uniqKey="Herz C">C. Herz</name>
</author>
<author><name sortKey="Ruf, P" uniqKey="Ruf P">P. Ruf</name>
</author>
<author><name sortKey="Stetter, R" uniqKey="Stetter R">R. Stetter</name>
</author>
<author><name sortKey="Lamy, E" uniqKey="Lamy E">E. Lamy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Victoni, T" uniqKey="Victoni T">T. Victoni</name>
</author>
<author><name sortKey="Salvator, H" uniqKey="Salvator H">H. Salvator</name>
</author>
<author><name sortKey="Abrial, C" uniqKey="Abrial C">C. Abrial</name>
</author>
<author><name sortKey="Brollo, M" uniqKey="Brollo M">M. Brollo</name>
</author>
<author><name sortKey="Porto, L C S" uniqKey="Porto L">L. C. S. Porto</name>
</author>
<author><name sortKey="Lagente, V" uniqKey="Lagente V">V. Lagente</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vrancic, M" uniqKey="Vrancic M">M. Vrancic</name>
</author>
<author><name sortKey="Banjanac, M" uniqKey="Banjanac M">M. Banjanac</name>
</author>
<author><name sortKey="Nujic, K" uniqKey="Nujic K">K. Nujic</name>
</author>
<author><name sortKey="Bosnar, M" uniqKey="Bosnar M">M. Bosnar</name>
</author>
<author><name sortKey="Murati, T" uniqKey="Murati T">T. Murati</name>
</author>
<author><name sortKey="Munic, V" uniqKey="Munic V">V. Munic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, M" uniqKey="Yang M">M. Yang</name>
</author>
<author><name sortKey="Kumar, R K" uniqKey="Kumar R">R. K. Kumar</name>
</author>
<author><name sortKey="Hansbro, P M" uniqKey="Hansbro P">P. M. Hansbro</name>
</author>
<author><name sortKey="Foster, P S" uniqKey="Foster P">P. S. Foster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yasutomi, M" uniqKey="Yasutomi M">M. Yasutomi</name>
</author>
<author><name sortKey="Ohshima, Y" uniqKey="Ohshima Y">Y. Ohshima</name>
</author>
<author><name sortKey="Omata, N" uniqKey="Omata N">N. Omata</name>
</author>
<author><name sortKey="Yamada, A" uniqKey="Yamada A">A. Yamada</name>
</author>
<author><name sortKey="Iwasaki, H" uniqKey="Iwasaki H">H. Iwasaki</name>
</author>
<author><name sortKey="Urasaki, Y" uniqKey="Urasaki Y">Y. Urasaki</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Physiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Front Physiol</journal-id>
<journal-id journal-id-type="publisher-id">Front. Physiol.</journal-id>
<journal-title-group><journal-title>Frontiers in Physiology</journal-title>
</journal-title-group>
<issn pub-type="epub">1664-042X</issn>
<publisher><publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">31632299</article-id>
<article-id pub-id-type="pmc">6783802</article-id>
<article-id pub-id-type="doi">10.3389/fphys.2019.01267</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Physiology</subject>
<subj-group><subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Bitter Taste Receptors (TAS2Rs) in Human Lung Macrophages: Receptor Expression and Inhibitory Effects of TAS2R Agonists</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Grassin-Delyle</surname>
<given-names>Stanislas</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="corresp" rid="c001"><sup>*</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/652066/overview"></uri>
</contrib>
<contrib contrib-type="author"><name><surname>Salvator</surname>
<given-names>Hélène</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mantov</surname>
<given-names>Nikola</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/714027/overview"></uri>
</contrib>
<contrib contrib-type="author"><name><surname>Abrial</surname>
<given-names>Charlotte</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Brollo</surname>
<given-names>Marion</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Faisy</surname>
<given-names>Christophe</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Naline</surname>
<given-names>Emmanuel</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Couderc</surname>
<given-names>Louis-Jean</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Devillier</surname>
<given-names>Philippe</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
<xref ref-type="corresp" rid="c002"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><sup>1</sup>
<institution>Department of Airway Diseases, Foch Hospital</institution>
,<addr-line>Suresnes</addr-line>
,<country>France</country>
</aff>
<aff id="aff2"><sup>2</sup>
<institution>INSERM UMR 1173, UFR Simone Veil - Santé, University Versailles Saint-Quentin, University of Paris-Saclay</institution>
,<addr-line>Montigny-le-Bretonneux</addr-line>
,<country>France</country>
</aff>
<aff id="aff3"><sup>3</sup>
<institution>Laboratory of Research in Respiratory Pharmacology–UPRES EA 220, Foch Hospital, University Versailles Saint-Quentin, University of Paris-Saclay</institution>
,<addr-line>Suresnes</addr-line>
,<country>France</country>
</aff>
<author-notes><fn fn-type="edited-by"><p>Edited by: Maik Behrens, Technical University of Munich, Germany</p>
</fn>
<fn fn-type="edited-by"><p>Reviewed by: Deepak A. Deshpande, Thomas Jefferson University, United States; Robert J. Lee, University of Pennsylvania, United States</p>
</fn>
<corresp id="c001">*Correspondence: Stanislas Grassin-Delyle, <email>s.grassindelyle@hopital-foch.org</email>
</corresp>
<corresp id="c002">Philippe Devillier, <email>p.devillier@hopital-foch.org</email>
</corresp>
<fn fn-type="other" id="fn004"><p>This article was submitted to Integrative Physiology, a section of the journal Frontiers in Physiology</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>02</day>
<month>10</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="collection"><year>2019</year>
</pub-date>
<volume>10</volume>
<elocation-id>1267</elocation-id>
<history><date date-type="received"><day>26</day>
<month>3</month>
<year>2019</year>
</date>
<date date-type="accepted"><day>19</day>
<month>9</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2019 Grassin-Delyle, Salvator, Mantov, Abrial, Brollo, Faisy, Naline, Couderc and Devillier.</copyright-statement>
<copyright-year>2019</copyright-year>
<copyright-holder>Grassin-Delyle, Salvator, Mantov, Abrial, Brollo, Faisy, Naline, Couderc and Devillier</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract><sec><title>Background</title>
<p>Bitter-taste receptors (TAS2Rs) are involved in airway relaxation but are also expressed in human blood leukocytes. We studied TAS2R expression and the effects of TAS2R agonists on the lipopolysaccharide (LPS)-induced cytokine release in human lung macrophages (LMs).</p>
</sec>
<sec><title>Methods</title>
<p>Lung macrophages were isolated from patients undergoing surgery for carcinoma. We used RT-qPCR to measure transcripts of 16 TAS2Rs (TAS2Rs 3/4/5/7/8/9/10/14/19/20/31/38/39/43/45 and 46) in unstimulated and LPS-stimulated (10 ng.mL<sup>–1</sup>
) LMs. The macrophages were also incubated with TAS2R agonists for 24 h. Supernatant levels of the cytokines TNF-α, CCL3, CXCL8 and IL-10 were measured using ELISAs.</p>
</sec>
<sec><title>Results</title>
<p>The transcripts of all 16 TAS2Rs were detected in macrophages. The addition of LPS led to an increase in the expression of most TAS2Rs, which was significant for TAS2R7 and 38. Although the promiscuous TAS2R agonists, quinine and denatonium, inhibited the LPS-induced release of TNF-α, CCL3 and CXCL8, diphenidol was inactive. Partially selective agonists (dapsone, colchicine, strychnine, and chloroquine) and selective agonists [erythromycin (TAS2R10), phenanthroline (TAS2R5), ofloxacin (TAS2R9), and carisoprodol (TAS2R14)] also suppressed the LPS-induced cytokine release. In contrast, two other agonists [sodium cromoglycate (TAS2R20) and saccharin (TAS2R31 and 43)] were inactive. TAS2R agonists suppressed IL-10 production – suggesting that this anti-inflammatory cytokine is not involved in the inhibition of cytokine production.</p>
</sec>
<sec><title>Conclusion</title>
<p>Human LMs expressed TAS2Rs. Experiments with TAS2R agonists’ suggested the involvement of TAS2Rs 3, 4, 5, 9, 10, 14, 30, 39 and 40 in the inhibition of cytokine production. TAS2Rs may constitute new drug targets in inflammatory obstructive lung disease.</p>
</sec>
</abstract>
<kwd-group><kwd>bitter taste receptor</kwd>
<kwd>human lung macrophage</kwd>
<kwd>parenchyma explant</kwd>
<kwd>cytokine</kwd>
<kwd>inflammation</kwd>
</kwd-group>
<funding-group><award-group><funding-source id="cn001">Chancellerie des Universités de Paris<named-content content-type="fundref-id">10.13039/501100010466</named-content>
</funding-source>
<award-id rid="cn001">Legs Gaston Poix</award-id>
</award-group>
</funding-group>
<counts><fig-count count="6"></fig-count>
<table-count count="2"></table-count>
<equation-count count="0"></equation-count>
<ref-count count="62"></ref-count>
<page-count count="13"></page-count>
<word-count count="0"></word-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>France</li>
</country>
</list>
<tree><country name="France"><noRegion><name sortKey="Grassin Delyle, Stanislas" sort="Grassin Delyle, Stanislas" uniqKey="Grassin Delyle S" first="Stanislas" last="Grassin-Delyle">Stanislas Grassin-Delyle</name>
</noRegion>
<name sortKey="Abrial, Charlotte" sort="Abrial, Charlotte" uniqKey="Abrial C" first="Charlotte" last="Abrial">Charlotte Abrial</name>
<name sortKey="Brollo, Marion" sort="Brollo, Marion" uniqKey="Brollo M" first="Marion" last="Brollo">Marion Brollo</name>
<name sortKey="Couderc, Louis Jean" sort="Couderc, Louis Jean" uniqKey="Couderc L" first="Louis-Jean" last="Couderc">Louis-Jean Couderc</name>
<name sortKey="Couderc, Louis Jean" sort="Couderc, Louis Jean" uniqKey="Couderc L" first="Louis-Jean" last="Couderc">Louis-Jean Couderc</name>
<name sortKey="Devillier, Philippe" sort="Devillier, Philippe" uniqKey="Devillier P" first="Philippe" last="Devillier">Philippe Devillier</name>
<name sortKey="Devillier, Philippe" sort="Devillier, Philippe" uniqKey="Devillier P" first="Philippe" last="Devillier">Philippe Devillier</name>
<name sortKey="Faisy, Christophe" sort="Faisy, Christophe" uniqKey="Faisy C" first="Christophe" last="Faisy">Christophe Faisy</name>
<name sortKey="Grassin Delyle, Stanislas" sort="Grassin Delyle, Stanislas" uniqKey="Grassin Delyle S" first="Stanislas" last="Grassin-Delyle">Stanislas Grassin-Delyle</name>
<name sortKey="Mantov, Nikola" sort="Mantov, Nikola" uniqKey="Mantov N" first="Nikola" last="Mantov">Nikola Mantov</name>
<name sortKey="Naline, Emmanuel" sort="Naline, Emmanuel" uniqKey="Naline E" first="Emmanuel" last="Naline">Emmanuel Naline</name>
<name sortKey="Naline, Emmanuel" sort="Naline, Emmanuel" uniqKey="Naline E" first="Emmanuel" last="Naline">Emmanuel Naline</name>
<name sortKey="Salvator, Helene" sort="Salvator, Helene" uniqKey="Salvator H" first="Hélène" last="Salvator">Hélène Salvator</name>
<name sortKey="Salvator, Helene" sort="Salvator, Helene" uniqKey="Salvator H" first="Hélène" last="Salvator">Hélène Salvator</name>
</country>
</tree>
</affiliations>
</record>
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