Combining use of Phillyrin and autophagy blocker alleviates laryngeal squamous cell carcinoma via AMPK/mTOR/p70S6K signaling
Identifieur interne : 000879 ( Pmc/Checkpoint ); précédent : 000878; suivant : 000880Combining use of Phillyrin and autophagy blocker alleviates laryngeal squamous cell carcinoma via AMPK/mTOR/p70S6K signaling
Auteurs : Da-Hua Wang [République populaire de Chine] ; Xi He [République populaire de Chine] ; Qing He [République populaire de Chine]Source :
- Bioscience Reports [ 0144-8463 ] ; 2019.
Abstract
Phillyrin (PHN), one of the major active constituents of
Url:
DOI: 10.1042/BSR20190459
PubMed: 31147451
PubMed Central: 6616054
Affiliations:
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PMC:6616054Le document en format XML
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<author><name sortKey="He, Qing" sort="He, Qing" uniqKey="He Q" first="Qing" last="He">Qing He</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Combining use of Phillyrin and autophagy blocker alleviates laryngeal squamous cell carcinoma via AMPK/mTOR/p70S6K signaling</title>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Otolaryngology, Xuzhou Municipal Hospital Affiliated with Xuzhou Medical University, Xuzhou 221002</wicri:regionArea>
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<author><name sortKey="He, Xi" sort="He, Xi" uniqKey="He X" first="Xi" last="He">Xi He</name>
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<author><name sortKey="He, Qing" sort="He, Qing" uniqKey="He Q" first="Qing" last="He">Qing He</name>
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<front><div type="abstract" xml:lang="en"><p>Phillyrin (PHN), one of the major active constituents of <italic>Forsythia suspensa</italic>
and <italic>F. koreana</italic>
, has been reported to produce antioxidant, antibacterial, anti-obesity and anti-inflammatory effects. However, no study has demonstrated the role of PHN in laryngeal squamous cell carcinoma (LSCC). We aimed to investigate the effects of PHN on the proliferation and apoptosis of HEp-2 cells. In the present study, PHN alone showed little effect on HEp-2 cell proliferation and apoptosis. Subsequent tests showed that PHN could largely enhance the level of autophagy on HEp-2 cells. Combining use of PHN and autophagy blockers including 3-methyladenine (3-MA) and chloroquine (CQ) significantly inhibited HEp-2 cell proliferation in a dose- and time-dependent manner and induced apoptosis after 24 h in a dose-dependent manner. Additionally, we found that the possible underlying molecular mechanism of PHN-induced autophagy might be through the AMPK/mTOR/p70S6K signaling pathway. Taken together, our study indicates that combining use of PHN and autophagy blockers may serve as a novel strategy in LSCC treatment.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biosci Rep</journal-id>
<journal-id journal-id-type="iso-abbrev">Biosci. Rep</journal-id>
<journal-id journal-id-type="hwp">ppbioscirep</journal-id>
<journal-id journal-id-type="publisher-id">BSR</journal-id>
<journal-title-group><journal-title>Bioscience Reports</journal-title>
</journal-title-group>
<issn pub-type="ppub">0144-8463</issn>
<issn pub-type="epub">1573-4935</issn>
<publisher><publisher-name>Portland Press Ltd.</publisher-name>
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<article-meta><article-id pub-id-type="pmid">31147451</article-id>
<article-id pub-id-type="pmc">6616054</article-id>
<article-id pub-id-type="doi">10.1042/BSR20190459</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject>
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<subj-group subj-group-type="overline"><subject>Research Article</subject>
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<subj-group subj-group-type="hwp-journal-coll"><subject>21</subject>
<subject>39</subject>
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<title-group><article-title>Combining use of Phillyrin and autophagy blocker alleviates laryngeal squamous cell carcinoma via AMPK/mTOR/p70S6K signaling</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0002-7841-4989</contrib-id>
<name><surname>Wang</surname>
<given-names>Da-hua</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="afn1">*</xref>
<xref ref-type="corresp" rid="COR1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>He</surname>
<given-names>Xi</given-names>
</name>
<xref ref-type="aff" rid="A2"><sup>2</sup>
</xref>
<xref ref-type="author-notes" rid="afn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>He</surname>
<given-names>Qing</given-names>
</name>
<xref ref-type="aff" rid="A3"><sup>3</sup>
</xref>
</contrib>
<aff id="A1"><label>1</label>
Department of Otolaryngology, Xuzhou Municipal Hospital Affiliated with Xuzhou Medical University, Xuzhou 221002, China</aff>
<aff id="A2"><label>2</label>
Department of Dermatology, Xuzhou Municipal Hospital Affiliated with Xuzhou Medical University, Xuzhou 221002, China</aff>
<aff id="A3"><label>3</label>
Department of Neurology, Xuzhou Municipal Hospital Affiliated with Xuzhou Medical University, Xuzhou 221002, China</aff>
</contrib-group>
<author-notes><corresp id="COR1"><bold>Correspondence:</bold>
Da-hua Wang (<email>jack_dahua_wang@163.com</email>
)</corresp>
<fn id="afn1" fn-type="equal"><label>*</label>
<p>These authors contributed equally to this work.</p>
</fn>
</author-notes>
<pub-date pub-type="epreprint"><day>29</day>
<month>5</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub"><day>20</day>
<month>6</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="collection"><day>28</day>
<month>6</month>
<year>2019</year>
</pub-date>
<volume>39</volume>
<issue>6</issue>
<elocation-id>BSR20190459</elocation-id>
<history><date date-type="received"><day>23</day>
<month>2</month>
<year>2019</year>
</date>
<date date-type="rev-recd"><day>07</day>
<month>5</month>
<year>2019</year>
</date>
<date date-type="accepted"><day>21</day>
<month>5</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>© 2019 The Author(s).</copyright-statement>
<copyright-year>2019</copyright-year>
<license license-type="open-access"><ali:license_ref>http://creativecommons.org/licenses/by/4.0/</ali:license_ref>
<license-p>This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License 4.0 (CC BY)</ext-link>
.</license-p>
</license>
</permissions>
<self-uri xlink:title="pdf" xlink:href="bsr-39-bsr20190459.pdf"></self-uri>
<abstract><p>Phillyrin (PHN), one of the major active constituents of <italic>Forsythia suspensa</italic>
and <italic>F. koreana</italic>
, has been reported to produce antioxidant, antibacterial, anti-obesity and anti-inflammatory effects. However, no study has demonstrated the role of PHN in laryngeal squamous cell carcinoma (LSCC). We aimed to investigate the effects of PHN on the proliferation and apoptosis of HEp-2 cells. In the present study, PHN alone showed little effect on HEp-2 cell proliferation and apoptosis. Subsequent tests showed that PHN could largely enhance the level of autophagy on HEp-2 cells. Combining use of PHN and autophagy blockers including 3-methyladenine (3-MA) and chloroquine (CQ) significantly inhibited HEp-2 cell proliferation in a dose- and time-dependent manner and induced apoptosis after 24 h in a dose-dependent manner. Additionally, we found that the possible underlying molecular mechanism of PHN-induced autophagy might be through the AMPK/mTOR/p70S6K signaling pathway. Taken together, our study indicates that combining use of PHN and autophagy blockers may serve as a novel strategy in LSCC treatment.</p>
</abstract>
<kwd-group><kwd>3-methyladenine</kwd>
<kwd>autophagy</kwd>
<kwd>chloroquine</kwd>
<kwd>laryngeal squamous cell carcinoma</kwd>
<kwd>Phillyrin</kwd>
</kwd-group>
<counts><page-count count="10"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Da Hua" sort="Wang, Da Hua" uniqKey="Wang D" first="Da-Hua" last="Wang">Da-Hua Wang</name>
</noRegion>
<name sortKey="He, Qing" sort="He, Qing" uniqKey="He Q" first="Qing" last="He">Qing He</name>
<name sortKey="He, Xi" sort="He, Xi" uniqKey="He X" first="Xi" last="He">Xi He</name>
</country>
</tree>
</affiliations>
</record>
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