Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system
Identifieur interne : 000669 ( Pmc/Checkpoint ); précédent : 000668; suivant : 000670Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system
Auteurs : Daisuke Yamada [Japon] ; Kenji Kawabe [Japon] ; Ikue Tosa [Japon] ; Shunpei Tsukamoto [Japon] ; Ryota Nakazato ; Miki Kou ; Koichi Fujikawa ; Saki Nakamura ; Mitsuaki Ono [Japon] ; Toshitaka Oohashi [Japon] ; Mari Kaneko ; Shioi Go ; Eiichi Hinoi ; Yukio Yoneda ; Takeshi Takarada [Japon]Source :
- Communications Biology [ 2399-3642 ] ; 2019.
Abstract
The pathophysiological role of mammalian target of rapamycin complex 1 (mTORC1) in neurodegenerative diseases is established, but possible therapeutic targets responsible for its activation in neurons must be explored. Here we identified solute carrier family 38a member 1 (SNAT1,
Url:
DOI: 10.1038/s42003-019-0582-4
PubMed: 31552299
PubMed Central: 6751179
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:6751179Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system</title><author><name sortKey="Yamada, Daisuke" sort="Yamada, Daisuke" uniqKey="Yamada D" first="Daisuke" last="Yamada">Daisuke Yamada</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Kawabe, Kenji" sort="Kawabe, Kenji" uniqKey="Kawabe K" first="Kenji" last="Kawabe">Kenji Kawabe</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Tosa, Ikue" sort="Tosa, Ikue" uniqKey="Tosa I" first="Ikue" last="Tosa">Ikue Tosa</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Tsukamoto, Shunpei" sort="Tsukamoto, Shunpei" uniqKey="Tsukamoto S" first="Shunpei" last="Tsukamoto">Shunpei Tsukamoto</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Nakazato, Ryota" sort="Nakazato, Ryota" uniqKey="Nakazato R" first="Ryota" last="Nakazato">Ryota Nakazato</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Kou, Miki" sort="Kou, Miki" uniqKey="Kou M" first="Miki" last="Kou">Miki Kou</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Fujikawa, Koichi" sort="Fujikawa, Koichi" uniqKey="Fujikawa K" first="Koichi" last="Fujikawa">Koichi Fujikawa</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Nakamura, Saki" sort="Nakamura, Saki" uniqKey="Nakamura S" first="Saki" last="Nakamura">Saki Nakamura</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Ono, Mitsuaki" sort="Ono, Mitsuaki" uniqKey="Ono M" first="Mitsuaki" last="Ono">Mitsuaki Ono</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Molecular Biology and Biochemistry,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Oohashi, Toshitaka" sort="Oohashi, Toshitaka" uniqKey="Oohashi T" first="Toshitaka" last="Oohashi">Toshitaka Oohashi</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Molecular Biology and Biochemistry,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Kaneko, Mari" sort="Kaneko, Mari" uniqKey="Kaneko M" first="Mari" last="Kaneko">Mari Kaneko</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000000094465255</institution-id><institution-id institution-id-type="GRID">grid.7597.c</institution-id><institution>Laboratory for Animal Resource Development Unit and Genetic Engineering Team,</institution><institution>RIKEN Center for Life Science Technologies,</institution></institution-wrap>2-2-3 Minatojima Minami, Chuou-ku, Kobe, Hyogo 650-0047 Japan</nlm:aff><wicri:noCountry code="subfield">Hyogo 650-0047 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Go, Shioi" sort="Go, Shioi" uniqKey="Go S" first="Shioi" last="Go">Shioi Go</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000000094465255</institution-id><institution-id institution-id-type="GRID">grid.7597.c</institution-id><institution>Laboratory for Animal Resource Development Unit and Genetic Engineering Team,</institution><institution>RIKEN Center for Life Science Technologies,</institution></institution-wrap>2-2-3 Minatojima Minami, Chuou-ku, Kobe, Hyogo 650-0047 Japan</nlm:aff><wicri:noCountry code="subfield">Hyogo 650-0047 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Hinoi, Eiichi" sort="Hinoi, Eiichi" uniqKey="Hinoi E" first="Eiichi" last="Hinoi">Eiichi Hinoi</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Yoneda, Yukio" sort="Yoneda, Yukio" uniqKey="Yoneda Y" first="Yukio" last="Yoneda">Yukio Yoneda</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Takarada, Takeshi" sort="Takarada, Takeshi" uniqKey="Takarada T" first="Takeshi" last="Takarada">Takeshi Takarada</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31552299</idno><idno type="pmc">6751179</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751179</idno><idno type="RBID">PMC:6751179</idno><idno type="doi">10.1038/s42003-019-0582-4</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000093</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000093</idno><idno type="wicri:Area/Pmc/Curation">000093</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000093</idno><idno type="wicri:Area/Pmc/Checkpoint">000669</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000669</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system</title><author><name sortKey="Yamada, Daisuke" sort="Yamada, Daisuke" uniqKey="Yamada D" first="Daisuke" last="Yamada">Daisuke Yamada</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Kawabe, Kenji" sort="Kawabe, Kenji" uniqKey="Kawabe K" first="Kenji" last="Kawabe">Kenji Kawabe</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Tosa, Ikue" sort="Tosa, Ikue" uniqKey="Tosa I" first="Ikue" last="Tosa">Ikue Tosa</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Tsukamoto, Shunpei" sort="Tsukamoto, Shunpei" uniqKey="Tsukamoto S" first="Shunpei" last="Tsukamoto">Shunpei Tsukamoto</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Nakazato, Ryota" sort="Nakazato, Ryota" uniqKey="Nakazato R" first="Ryota" last="Nakazato">Ryota Nakazato</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Kou, Miki" sort="Kou, Miki" uniqKey="Kou M" first="Miki" last="Kou">Miki Kou</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Fujikawa, Koichi" sort="Fujikawa, Koichi" uniqKey="Fujikawa K" first="Koichi" last="Fujikawa">Koichi Fujikawa</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Nakamura, Saki" sort="Nakamura, Saki" uniqKey="Nakamura S" first="Saki" last="Nakamura">Saki Nakamura</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Ono, Mitsuaki" sort="Ono, Mitsuaki" uniqKey="Ono M" first="Mitsuaki" last="Ono">Mitsuaki Ono</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Molecular Biology and Biochemistry,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Oohashi, Toshitaka" sort="Oohashi, Toshitaka" uniqKey="Oohashi T" first="Toshitaka" last="Oohashi">Toshitaka Oohashi</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Molecular Biology and Biochemistry,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author><author><name sortKey="Kaneko, Mari" sort="Kaneko, Mari" uniqKey="Kaneko M" first="Mari" last="Kaneko">Mari Kaneko</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000000094465255</institution-id><institution-id institution-id-type="GRID">grid.7597.c</institution-id><institution>Laboratory for Animal Resource Development Unit and Genetic Engineering Team,</institution><institution>RIKEN Center for Life Science Technologies,</institution></institution-wrap>2-2-3 Minatojima Minami, Chuou-ku, Kobe, Hyogo 650-0047 Japan</nlm:aff><wicri:noCountry code="subfield">Hyogo 650-0047 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Go, Shioi" sort="Go, Shioi" uniqKey="Go S" first="Shioi" last="Go">Shioi Go</name><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000000094465255</institution-id><institution-id institution-id-type="GRID">grid.7597.c</institution-id><institution>Laboratory for Animal Resource Development Unit and Genetic Engineering Team,</institution><institution>RIKEN Center for Life Science Technologies,</institution></institution-wrap>2-2-3 Minatojima Minami, Chuou-ku, Kobe, Hyogo 650-0047 Japan</nlm:aff><wicri:noCountry code="subfield">Hyogo 650-0047 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Hinoi, Eiichi" sort="Hinoi, Eiichi" uniqKey="Hinoi E" first="Eiichi" last="Hinoi">Eiichi Hinoi</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Yoneda, Yukio" sort="Yoneda, Yukio" uniqKey="Yoneda Y" first="Yukio" last="Yoneda">Yukio Yoneda</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</nlm:aff><wicri:noCountry code="subfield">Ishikawa 920-1192 Japan</wicri:noCountry></affiliation></author><author><name sortKey="Takarada, Takeshi" sort="Takarada, Takeshi" uniqKey="Takarada T" first="Takeshi" last="Takarada">Takeshi Takarada</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Okayama</wicri:regionArea></affiliation></author></analytic><series><title level="j">Communications Biology</title><idno type="eISSN">2399-3642</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The pathophysiological role of mammalian target of rapamycin complex 1 (mTORC1) in neurodegenerative diseases is established, but possible therapeutic targets responsible for its activation in neurons must be explored. Here we identified solute carrier family 38a member 1 (SNAT1, <italic>Slc38a1</italic>) as a positive regulator of mTORC1 in neurons. <italic>Slc38a1</italic><sup><italic>flox/flox</italic></sup> and <italic>Synapsin I-Cre</italic> mice were crossed to generate mutant mice in which <italic>Slc38a1</italic> was selectively deleted in neurons. Measurement of 2,3,5-triphenyltetrazolium chloride (TTC) or the MAP2-negative area in a mouse model of middle cerebral artery occlusion (MCAO) revealed that <italic>Slc38a1</italic> deficiency decreased infarct size. We found a transient increase in the phosphorylation of p70S6k1 (pp70S6k1) and a suppressive effect of rapamycin on infarct size in MCAO mice. Autophagy inhibitors completely mitigated the suppressive effect of SNAT1 deficiency on neuronal cell death under in vitro stroke culture conditions. These results demonstrate that SNAT1 promoted ischemic brain damage via mTOR-autophagy system.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Nixon, Ra" uniqKey="Nixon R">RA Nixon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lipton, Jo" uniqKey="Lipton J">JO Lipton</name></author><author><name sortKey="Sahin, M" uniqKey="Sahin M">M Sahin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Switon, K" uniqKey="Switon K">K Switon</name></author><author><name sortKey="Kotulska, K" uniqKey="Kotulska K">K Kotulska</name></author><author><name sortKey="Janusz Kaminska, A" uniqKey="Janusz Kaminska A">A Janusz-Kaminska</name></author><author><name sortKey="Zmorzynska, J" uniqKey="Zmorzynska J">J Zmorzynska</name></author><author><name sortKey="Jaworski, J" uniqKey="Jaworski J">J Jaworski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zoncu, R" uniqKey="Zoncu R">R Zoncu</name></author><author><name sortKey="Efeyan, A" uniqKey="Efeyan A">A Efeyan</name></author><author><name sortKey="Sabatini, Dm" uniqKey="Sabatini D">DM Sabatini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Papadakis, M" uniqKey="Papadakis M">M Papadakis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bockaert, J" uniqKey="Bockaert J">J Bockaert</name></author><author><name sortKey="Marin, P" uniqKey="Marin P">P Marin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bove, J" uniqKey="Bove J">J Bove</name></author><author><name sortKey="Martinez Vicente, M" uniqKey="Martinez Vicente M">M Martinez-Vicente</name></author><author><name sortKey="Vila, M" uniqKey="Vila M">M Vila</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duran, Rv" uniqKey="Duran R">RV Duran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jewell, Jl" uniqKey="Jewell J">JL Jewell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Sg" uniqKey="Kim S">SG Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicklin, P" uniqKey="Nicklin P">P Nicklin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, L" uniqKey="Lin L">L Lin</name></author><author><name sortKey="Yee, Sw" uniqKey="Yee S">SW Yee</name></author><author><name sortKey="Kim, Rb" uniqKey="Kim R">RB Kim</name></author><author><name sortKey="Giacomini, Km" uniqKey="Giacomini K">KM Giacomini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pochini, L" uniqKey="Pochini L">L Pochini</name></author><author><name sortKey="Scalise, M" uniqKey="Scalise M">M Scalise</name></author><author><name sortKey="Galluccio, M" uniqKey="Galluccio M">M Galluccio</name></author><author><name sortKey="Indiveri, C" uniqKey="Indiveri C">C Indiveri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bagchi, S" uniqKey="Bagchi S">S Bagchi</name></author><author><name sortKey="Baomar, Ha" uniqKey="Baomar H">HA Baomar</name></author><author><name sortKey="Al Walai, S" uniqKey="Al Walai S">S Al-Walai</name></author><author><name sortKey="Al Sadi, S" uniqKey="Al Sadi S">S Al-Sadi</name></author><author><name sortKey="Fredriksson, R" uniqKey="Fredriksson R">R Fredriksson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hagglund, Mg" uniqKey="Hagglund M">MG Hagglund</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hagglund, Mg" uniqKey="Hagglund M">MG Hagglund</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mackenzie, B" uniqKey="Mackenzie B">B Mackenzie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elorza, A" uniqKey="Elorza A">A Elorza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kurayama, R" uniqKey="Kurayama R">R Kurayama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Solbu, Tt" uniqKey="Solbu T">TT Solbu</name></author><author><name sortKey="Bjorkmo, M" uniqKey="Bjorkmo M">M Bjorkmo</name></author><author><name sortKey="Berghuis, P" uniqKey="Berghuis P">P Berghuis</name></author><author><name sortKey="Harkany, T" uniqKey="Harkany T">T Harkany</name></author><author><name sortKey="Chaudhry, Fa" uniqKey="Chaudhry F">FA Chaudhry</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meikle, L" uniqKey="Meikle L">L Meikle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, E" uniqKey="Yuan E">E Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buckley, Km" uniqKey="Buckley K">KM Buckley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, L" uniqKey="Xie L">L Xie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, M" uniqKey="Wu M">M Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maiese, K" uniqKey="Maiese K">K Maiese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kondoh, N" uniqKey="Kondoh N">N Kondoh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, K" uniqKey="Wang K">K Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, Lw" uniqKey="Jin L">LW Jin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menon, S" uniqKey="Menon S">S Menon</name></author><author><name sortKey="Manning, Bd" uniqKey="Manning B">BD Manning</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hegazy, Am" uniqKey="Hegazy A">AM Hegazy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamada, D" uniqKey="Yamada D">D Yamada</name></author><author><name sortKey="Kawahara, K" uniqKey="Kawahara K">K Kawahara</name></author><author><name sortKey="Maeda, T" uniqKey="Maeda T">T Maeda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamada, D" uniqKey="Yamada D">D Yamada</name></author><author><name sortKey="Takahashi, K" uniqKey="Takahashi K">K Takahashi</name></author><author><name sortKey="Kawahara, K" uniqKey="Kawahara K">K Kawahara</name></author><author><name sortKey="Maeda, T" uniqKey="Maeda T">T Maeda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Broer, A" uniqKey="Broer A">A Broer</name></author><author><name sortKey="Rahimi, F" uniqKey="Rahimi F">F Rahimi</name></author><author><name sortKey="Broer, S" uniqKey="Broer S">S Broer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogura, M" uniqKey="Ogura M">M Ogura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, R" uniqKey="Xie R">R Xie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, R" uniqKey="Xie R">R Xie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xie, R" uniqKey="Xie R">R Xie</name></author><author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name></author><author><name sortKey="Ji, X" uniqKey="Ji X">X Ji</name></author><author><name sortKey="Zhao, H" uniqKey="Zhao H">H Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, Qw" uniqKey="Fan Q">QW Fan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Prat, L" uniqKey="Garcia Prat L">L Garcia-Prat</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mowers, Ee" uniqKey="Mowers E">EE Mowers</name></author><author><name sortKey="Sharifi, Mn" uniqKey="Sharifi M">MN Sharifi</name></author><author><name sortKey="Macleod, Kf" uniqKey="Macleod K">KF Macleod</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parks, Sk" uniqKey="Parks S">SK Parks</name></author><author><name sortKey="Chiche, J" uniqKey="Chiche J">J Chiche</name></author><author><name sortKey="Pouyssegur, J" uniqKey="Pouyssegur J">J Pouyssegur</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takamura, A" uniqKey="Takamura A">A Takamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name></author><author><name sortKey="Bian, H" uniqKey="Bian H">H Bian</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L Guo</name></author><author><name sortKey="Zhu, H" uniqKey="Zhu H">H Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dai, Sh" uniqKey="Dai S">SH Dai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shi, Zy" uniqKey="Shi Z">ZY Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Li" uniqKey="Wang L">Li Wang</name></author><author><name sortKey="Wang, Peng" uniqKey="Wang P">Peng Wang</name></author><author><name sortKey="Dong, He" uniqKey="Dong H">He Dong</name></author><author><name sortKey="Wang, Shiduan" uniqKey="Wang S">Shiduan Wang</name></author><author><name sortKey="Chu, Haichen" uniqKey="Chu H">Haichen Chu</name></author><author><name sortKey="Yan, Wei" uniqKey="Yan W">Wei Yan</name></author><author><name sortKey="Zhang, Xue" uniqKey="Zhang X">Xue Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fang, Zongping" uniqKey="Fang Z">Zongping Fang</name></author><author><name sortKey="Feng, Yun" uniqKey="Feng Y">Yun Feng</name></author><author><name sortKey="Li, Yuheng" uniqKey="Li Y">Yuheng Li</name></author><author><name sortKey="Deng, Jiao" uniqKey="Deng J">Jiao Deng</name></author><author><name sortKey="Nie, Huang" uniqKey="Nie H">Huang Nie</name></author><author><name sortKey="Yang, Qianzhi" uniqKey="Yang Q">Qianzhi Yang</name></author><author><name sortKey="Wang, Shiquan" uniqKey="Wang S">Shiquan Wang</name></author><author><name sortKey="Dong, Hailong" uniqKey="Dong H">Hailong Dong</name></author><author><name sortKey="Xiong, Lize" uniqKey="Xiong L">Lize Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, Y" uniqKey="Guo Y">Y Guo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y Zhu</name></author><author><name sortKey="Shui, M" uniqKey="Shui M">M Shui</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Hu, W" uniqKey="Hu W">W Hu</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Yueyang" uniqKey="Liu Y">Yueyang Liu</name></author><author><name sortKey="Xue, Xue" uniqKey="Xue X">Xue Xue</name></author><author><name sortKey="Zhang, Haotian" uniqKey="Zhang H">Haotian Zhang</name></author><author><name sortKey="Che, Xiaohang" uniqKey="Che X">Xiaohang Che</name></author><author><name sortKey="Luo, Jing" uniqKey="Luo J">Jing Luo</name></author><author><name sortKey="Wang, Ping" uniqKey="Wang P">Ping Wang</name></author><author><name sortKey="Xu, Jiaoyan" uniqKey="Xu J">Jiaoyan Xu</name></author><author><name sortKey="Xing, Zheng" uniqKey="Xing Z">Zheng Xing</name></author><author><name sortKey="Yuan, Linlin" uniqKey="Yuan L">Linlin Yuan</name></author><author><name sortKey="Liu, Yinglu" uniqKey="Liu Y">Yinglu Liu</name></author><author><name sortKey="Fu, Xiaoxiao" uniqKey="Fu X">Xiaoxiao Fu</name></author><author><name sortKey="Su, Dongmei" uniqKey="Su D">Dongmei Su</name></author><author><name sortKey="Sun, Shibo" uniqKey="Sun S">Shibo Sun</name></author><author><name sortKey="Zhang, Haonan" uniqKey="Zhang H">Haonan Zhang</name></author><author><name sortKey="Wu, Chunfu" uniqKey="Wu C">Chunfu Wu</name></author><author><name sortKey="Yang, Jingyu" uniqKey="Yang J">Jingyu Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yagi, T" uniqKey="Yagi T">T Yagi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kwiatkowski, Dj" uniqKey="Kwiatkowski D">DJ Kwiatkowski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Longa, Ez" uniqKey="Longa E">EZ Longa</name></author><author><name sortKey="Weinstein, Pr" uniqKey="Weinstein P">PR Weinstein</name></author><author><name sortKey="Carlson, S" uniqKey="Carlson S">S Carlson</name></author><author><name sortKey="Cummins, R" uniqKey="Cummins R">R Cummins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takarada, T" uniqKey="Takarada T">T Takarada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takarada, T" uniqKey="Takarada T">T Takarada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lopatina, O" uniqKey="Lopatina O">O Lopatina</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Commun Biol</journal-id><journal-id journal-id-type="iso-abbrev">Commun Biol</journal-id><journal-title-group><journal-title>Communications Biology</journal-title></journal-title-group><issn pub-type="epub">2399-3642</issn><publisher><publisher-name>Nature Publishing Group UK</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31552299</article-id><article-id pub-id-type="pmc">6751179</article-id><article-id pub-id-type="publisher-id">582</article-id><article-id pub-id-type="doi">10.1038/s42003-019-0582-4</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Yamada</surname><given-names>Daisuke</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kawabe</surname><given-names>Kenji</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Tosa</surname><given-names>Ikue</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Tsukamoto</surname><given-names>Shunpei</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Nakazato</surname><given-names>Ryota</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Kou</surname><given-names>Miki</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Fujikawa</surname><given-names>Koichi</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Nakamura</surname><given-names>Saki</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Ono</surname><given-names>Mitsuaki</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Oohashi</surname><given-names>Toshitaka</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Kaneko</surname><given-names>Mari</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Go</surname><given-names>Shioi</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Hinoi</surname><given-names>Eiichi</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-4624-177X</contrib-id><name><surname>Yoneda</surname><given-names>Yukio</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Takarada</surname><given-names>Takeshi</given-names></name><address><email>takarada@okayama-u.ac.jp</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Regenerative Science,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2308 3329</institution-id><institution-id institution-id-type="GRID">grid.9707.9</institution-id><institution>Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences,</institution><institution>Kanazawa University Graduate School,</institution></institution-wrap>Kanazawa, Ishikawa 920-1192 Japan</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1302 4472</institution-id><institution-id institution-id-type="GRID">grid.261356.5</institution-id><institution>Department of Molecular Biology and Biochemistry,</institution><institution>Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,</institution></institution-wrap>Okayama, 700-8558 Japan</aff><aff id="Aff4"><label>4</label><institution-wrap><institution-id institution-id-type="ISNI">0000000094465255</institution-id><institution-id institution-id-type="GRID">grid.7597.c</institution-id><institution>Laboratory for Animal Resource Development Unit and Genetic Engineering Team,</institution><institution>RIKEN Center for Life Science Technologies,</institution></institution-wrap>2-2-3 Minatojima Minami, Chuou-ku, Kobe, Hyogo 650-0047 Japan</aff></contrib-group><pub-date pub-type="epub"><day>18</day><month>9</month><year>2019</year></pub-date><pub-date pub-type="pmc-release"><day>18</day><month>9</month><year>2019</year></pub-date><pub-date pub-type="collection"><year>2019</year></pub-date><volume>2</volume><elocation-id>346</elocation-id><history><date date-type="received"><day>29</day><month>8</month><year>2018</year></date><date date-type="accepted"><day>19</day><month>8</month><year>2019</year></date></history><permissions><copyright-statement>© The Author(s) 2019</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold> This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">The pathophysiological role of mammalian target of rapamycin complex 1 (mTORC1) in neurodegenerative diseases is established, but possible therapeutic targets responsible for its activation in neurons must be explored. Here we identified solute carrier family 38a member 1 (SNAT1, <italic>Slc38a1</italic>) as a positive regulator of mTORC1 in neurons. <italic>Slc38a1</italic><sup><italic>flox/flox</italic></sup> and <italic>Synapsin I-Cre</italic> mice were crossed to generate mutant mice in which <italic>Slc38a1</italic> was selectively deleted in neurons. Measurement of 2,3,5-triphenyltetrazolium chloride (TTC) or the MAP2-negative area in a mouse model of middle cerebral artery occlusion (MCAO) revealed that <italic>Slc38a1</italic> deficiency decreased infarct size. We found a transient increase in the phosphorylation of p70S6k1 (pp70S6k1) and a suppressive effect of rapamycin on infarct size in MCAO mice. Autophagy inhibitors completely mitigated the suppressive effect of SNAT1 deficiency on neuronal cell death under in vitro stroke culture conditions. These results demonstrate that SNAT1 promoted ischemic brain damage via mTOR-autophagy system.</p></abstract><abstract id="Abs2" abstract-type="web-summary"><p id="Par2">Yamada et al. demonstrate that solute carrier family 38a member 1 (SNAT1, Slc38a1) is as a positive regulator of mTORC1 that promotes neuronal cell death, using neuron-specific <italic>Slc38a1</italic> knockout mice. This study shows that SNAT1 exacerbates ischemic brain damage through mTOR-mediated suppression of autophagy.</p></abstract><kwd-group kwd-group-type="npg-subject"><title>Subject terms</title><kwd>Cell death in the nervous system</kwd><kwd>Neurodegeneration</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">https://doi.org/10.13039/501100001700</institution-id><institution>Ministry of Education, Culture, Sports, Science and Technology (MEXT)</institution></institution-wrap></funding-source><award-id>26117507</award-id><award-id>16H01332</award-id><principal-award-recipient><name><surname>Takarada</surname><given-names>Takeshi</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2018</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Fujikawa, Koichi" sort="Fujikawa, Koichi" uniqKey="Fujikawa K" first="Koichi" last="Fujikawa">Koichi Fujikawa</name><name sortKey="Go, Shioi" sort="Go, Shioi" uniqKey="Go S" first="Shioi" last="Go">Shioi Go</name><name sortKey="Hinoi, Eiichi" sort="Hinoi, Eiichi" uniqKey="Hinoi E" first="Eiichi" last="Hinoi">Eiichi Hinoi</name><name sortKey="Kaneko, Mari" sort="Kaneko, Mari" uniqKey="Kaneko M" first="Mari" last="Kaneko">Mari Kaneko</name><name sortKey="Kou, Miki" sort="Kou, Miki" uniqKey="Kou M" first="Miki" last="Kou">Miki Kou</name><name sortKey="Nakamura, Saki" sort="Nakamura, Saki" uniqKey="Nakamura S" first="Saki" last="Nakamura">Saki Nakamura</name><name sortKey="Nakazato, Ryota" sort="Nakazato, Ryota" uniqKey="Nakazato R" first="Ryota" last="Nakazato">Ryota Nakazato</name><name sortKey="Yoneda, Yukio" sort="Yoneda, Yukio" uniqKey="Yoneda Y" first="Yukio" last="Yoneda">Yukio Yoneda</name></noCountry><country name="Japon"><noRegion><name sortKey="Yamada, Daisuke" sort="Yamada, Daisuke" uniqKey="Yamada D" first="Daisuke" last="Yamada">Daisuke Yamada</name></noRegion><name sortKey="Kawabe, Kenji" sort="Kawabe, Kenji" uniqKey="Kawabe K" first="Kenji" last="Kawabe">Kenji Kawabe</name><name sortKey="Ono, Mitsuaki" sort="Ono, Mitsuaki" uniqKey="Ono M" first="Mitsuaki" last="Ono">Mitsuaki Ono</name><name sortKey="Oohashi, Toshitaka" sort="Oohashi, Toshitaka" uniqKey="Oohashi T" first="Toshitaka" last="Oohashi">Toshitaka Oohashi</name><name sortKey="Takarada, Takeshi" sort="Takarada, Takeshi" uniqKey="Takarada T" first="Takeshi" last="Takarada">Takeshi Takarada</name><name sortKey="Tosa, Ikue" sort="Tosa, Ikue" uniqKey="Tosa I" first="Ikue" last="Tosa">Ikue Tosa</name><name sortKey="Tsukamoto, Shunpei" sort="Tsukamoto, Shunpei" uniqKey="Tsukamoto S" first="Shunpei" last="Tsukamoto">Shunpei Tsukamoto</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000669 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000669 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:6751179
|texte= Inhibition of the glutamine transporter SNAT1 confers neuroprotection in mice by modulating the mTOR-autophagy system
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:31552299" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |