ChloroquineV1, PascalFrancis, Curation, bibRecord, 000051

In vitro antiplasmodial activity and cytotoxicity of nine plants traditionally used in Gabon

Identifieur interne : 000051 ( PascalFrancis/Curation ); précédent : 000050; suivant : 000052

In vitro antiplasmodial activity and cytotoxicity of nine plants traditionally used in Gabon

Auteurs : Jean Bernard Lekana-Douki [Gabon] ; Jean Bernard Bongui [Gabon] ; Sandrine Lydie Oyegue Liabagui [Gabon] ; Sonya Estelle Zang Edou [Gabon] ; Rafika Zatra [Gabon] ; Ulrich Bisvigou [Gabon] ; Pierre Druilhe [France] ; Jacques Lebibi [Gabon] ; Fousseyni Samba Toure Ndouo [Gabon] ; Maryvonne Kombila [Gabon]

Source :

Journal of ethnopharmacology [ 0378-8741 ] ; 2011.

RBID : Pascal:11-0146176

Descripteurs français

Pascal (Inist)
- Activité biologique, In vitro, Antipaludique, Cytotoxicité, Plante médicinale, Médecine traditionnelle, Gabon, Plasmodium falciparum, Pharmacognosie, Origine végétale, Antiparasitaire.
Wicri :
- geographic : Gabon.
- topic : Plante médicinale.

English descriptors

KwdEn :
- Antimalarial, Biological activity, Cytotoxicity, Folk medicine, Gabon, In vitro, Medicinal plant, Parasiticide, Pharmacognosy, Plant origin, Plasmodium falciparum.

Abstract

Aim of the study: As part of a project to identify new compounds active on malarial parasites, we tested the in vitro antiplasmodial activity of nine plants traditionally used to treat malaria symptoms in Haut-Ogooué Province, South-East Gabon. Materials and methods: Dichloromethane and methanolic extracts of each plant were tested for their antiplasmodial activity on two chloroquine-resistant strains of Plasmodium falciparum (FCB and W2), based on lactate dehydrogenase activity. Cytotoxicity was assessed with the MTT test on MRC-5 human diploid embryonic lung cells. Results: The methanolic extract of Staudtia gabonensis and the dichloromethane extract of Adhatoda latibracteata showed high antiplasmodial activity (IC₅₀ < 1 μg/ml) and low cytotoxicity, with selectivity indexes of about 58.25 and 16.43, respectively. The methanolic extract of Monodora myristica and the dichloromethane extract of Afromomum giganteum also showed promising activity (1 < IC₅₀ < 10 μg/ml) and low cytotoxicity, with selectivity indexes about 15.70 and 12.48, respectively. Dichloromethane extracts of Monodora myristica and Leonotis Africana showed moderate activity (1050<40μg/ml), with selectivity indexes about 6.07 and 28.89, respectively. Both extracts of Culcasia lancifolia had IC₅₀ values of 10-40 μg/ml but high cytotoxicity (selectivity indexes <2.77). The methanolic extract of Dorstenia klaineana had moderate antiplasmodial activity (IC₅₀ around 17 μg/ml) but strong cytotoxicity (0.43 μg/ml), giving a selectivity index of about 0.03. Conclusions: Most extracts of nine selected plants traditionally used to treat malaria in Gabon had interesting antiplasmodial activity in vitro. This supports continued investigations of traditional medicines in the search for new antimalarial agents. The compounds responsible for the observed antiplasmodial effects are under investigation.

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A11	`01`	`1`		`@1 LEKANA-DOUKI (Jean Bernard)`
A11	`02`	`1`		`@1 BONGUI (Jean Bernard)`
A11	`03`	`1`		`@1 OYEGUE LIABAGUI (Sandrine Lydie)`
A11	`04`	`1`		`@1 EDOU (Sonya Estelle Zang)`
A11	`05`	`1`		`@1 ZATRA (Rafika)`
A11	`06`	`1`		`@1 BISVIGOU (Ulrich)`
A11	`07`	`1`		`@1 DRUILHE (Pierre)`
A11	`08`	`1`		`@1 LEBIBI (Jacques)`
A11	`09`	`1`		`@1 TOURE NDOUO (Fousseyni Samba)`
A11	`10`	`1`		`@1 KOMBILA (Maryvonne)`
A14	`01`			`@1 Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769 @2 Franceville @3 GAB @Z 1 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 9 aut.`
A14	`02`			`@1 Unité de Recherches en chimie, Faculté des Sciences, Université des Sciences et Techniques de Masuku, B.P. 943 @2 Franceville @3 GAB @Z 2 aut. @Z 8 aut.`
A14	`03`			`@1 Département de Parasitologie-Mycologie Médecine Tropicale, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009 @2 Libreville @3 GAB @Z 1 aut. @Z 3 aut. @Z 10 aut.`
A14	`04`			`@1 Departement de Santé Publique et de Médecine légale et du travail, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009 @2 Libreville @3 GAB @Z 6 aut.`
A14	`05`			`@1 Biochemical Parasitology Unit, Institut Pasteur, 25 rue du docteur Roux @2 75724 Paris @3 FRA @Z 7 aut.`
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C01	`01`		`ENG`	@0 Aim of the study: As part of a project to identify new compounds active on malarial parasites, we tested the in vitro antiplasmodial activity of nine plants traditionally used to treat malaria symptoms in Haut-Ogooué Province, South-East Gabon. Materials and methods: Dichloromethane and methanolic extracts of each plant were tested for their antiplasmodial activity on two chloroquine-resistant strains of Plasmodium falciparum (FCB and W2), based on lactate dehydrogenase activity. Cytotoxicity was assessed with the MTT test on MRC-5 human diploid embryonic lung cells. Results: The methanolic extract of Staudtia gabonensis and the dichloromethane extract of Adhatoda latibracteata showed high antiplasmodial activity (IC₅₀ < 1 μg/ml) and low cytotoxicity, with selectivity indexes of about 58.25 and 16.43, respectively. The methanolic extract of Monodora myristica and the dichloromethane extract of Afromomum giganteum also showed promising activity (1 < IC₅₀ < 10 μg/ml) and low cytotoxicity, with selectivity indexes about 15.70 and 12.48, respectively. Dichloromethane extracts of Monodora myristica and Leonotis Africana showed moderate activity (10<IC₅₀<40μg/ml), with selectivity indexes about 6.07 and 28.89, respectively. Both extracts of Culcasia lancifolia had IC₅₀ values of 10-40 μg/ml but high cytotoxicity (selectivity indexes <2.77). The methanolic extract of Dorstenia klaineana had moderate antiplasmodial activity (IC₅₀ around 17 μg/ml) but strong cytotoxicity (0.43 μg/ml), giving a selectivity index of about 0.03. Conclusions: Most extracts of nine selected plants traditionally used to treat malaria in Gabon had interesting antiplasmodial activity in vitro. This supports continued investigations of traditional medicines in the search for new antimalarial agents. The compounds responsible for the observed antiplasmodial effects are under investigation.
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C03	`08`	`X`	`FRE`	`@0 Plasmodium falciparum @2 NS @5 08`
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C03	`10`	`X`	`SPA`	`@0 Origen vegetal @5 23`
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C07	`02`	`X`	`FRE`	`@0 Apicomplexa @2 NS`
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C07	`03`	`X`	`FRE`	`@0 Protozoa @2 NS`
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N21				`@1 094`
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Le document en format XML

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<author><name sortKey="Lekana Douki, Jean Bernard" sort="Lekana Douki, Jean Bernard" uniqKey="Lekana Douki J" first="Jean Bernard" last="Lekana-Douki">Jean Bernard Lekana-Douki</name>
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<author><name sortKey="Edou, Sonya Estelle Zang" sort="Edou, Sonya Estelle Zang" uniqKey="Edou S" first="Sonya Estelle Zang" last="Edou">Sonya Estelle Zang Edou</name>
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<author><name sortKey="Zatra, Rafika" sort="Zatra, Rafika" uniqKey="Zatra R" first="Rafika" last="Zatra">Rafika Zatra</name>
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<author><name sortKey="Bisvigou, Ulrich" sort="Bisvigou, Ulrich" uniqKey="Bisvigou U" first="Ulrich" last="Bisvigou">Ulrich Bisvigou</name>
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<author><name sortKey="Lebibi, Jacques" sort="Lebibi, Jacques" uniqKey="Lebibi J" first="Jacques" last="Lebibi">Jacques Lebibi</name>
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<author><name sortKey="Toure Ndouo, Fousseyni Samba" sort="Toure Ndouo, Fousseyni Samba" uniqKey="Toure Ndouo F" first="Fousseyni Samba" last="Toure Ndouo">Fousseyni Samba Toure Ndouo</name>
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<author><name sortKey="Kombila, Maryvonne" sort="Kombila, Maryvonne" uniqKey="Kombila M" first="Maryvonne" last="Kombila">Maryvonne Kombila</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">In vitro antiplasmodial activity and cytotoxicity of nine plants traditionally used in Gabon</title>
<author><name sortKey="Lekana Douki, Jean Bernard" sort="Lekana Douki, Jean Bernard" uniqKey="Lekana Douki J" first="Jean Bernard" last="Lekana-Douki">Jean Bernard Lekana-Douki</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769</s1>
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<author><name sortKey="Bongui, Jean Bernard" sort="Bongui, Jean Bernard" uniqKey="Bongui J" first="Jean Bernard" last="Bongui">Jean Bernard Bongui</name>
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<author><name sortKey="Oyegue Liabagui, Sandrine Lydie" sort="Oyegue Liabagui, Sandrine Lydie" uniqKey="Oyegue Liabagui S" first="Sandrine Lydie" last="Oyegue Liabagui">Sandrine Lydie Oyegue Liabagui</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769</s1>
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<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Département de Parasitologie-Mycologie Médecine Tropicale, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009</s1>
<s2>Libreville</s2>
<s3>GAB</s3>
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<author><name sortKey="Edou, Sonya Estelle Zang" sort="Edou, Sonya Estelle Zang" uniqKey="Edou S" first="Sonya Estelle Zang" last="Edou">Sonya Estelle Zang Edou</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769</s1>
<s2>Franceville</s2>
<s3>GAB</s3>
<sZ>1 aut.</sZ>
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<country>Gabon</country>
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<author><name sortKey="Zatra, Rafika" sort="Zatra, Rafika" uniqKey="Zatra R" first="Rafika" last="Zatra">Rafika Zatra</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769</s1>
<s2>Franceville</s2>
<s3>GAB</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Gabon</country>
</affiliation>
</author>
<author><name sortKey="Bisvigou, Ulrich" sort="Bisvigou, Ulrich" uniqKey="Bisvigou U" first="Ulrich" last="Bisvigou">Ulrich Bisvigou</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769</s1>
<s2>Franceville</s2>
<s3>GAB</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Gabon</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Departement de Santé Publique et de Médecine légale et du travail, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009</s1>
<s2>Libreville</s2>
<s3>GAB</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Gabon</country>
</affiliation>
</author>
<author><name sortKey="Druilhe, Pierre" sort="Druilhe, Pierre" uniqKey="Druilhe P" first="Pierre" last="Druilhe">Pierre Druilhe</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Biochemical Parasitology Unit, Institut Pasteur, 25 rue du docteur Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Lebibi, Jacques" sort="Lebibi, Jacques" uniqKey="Lebibi J" first="Jacques" last="Lebibi">Jacques Lebibi</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Unité de Recherches en chimie, Faculté des Sciences, Université des Sciences et Techniques de Masuku, B.P. 943</s1>
<s2>Franceville</s2>
<s3>GAB</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Gabon</country>
</affiliation>
</author>
<author><name sortKey="Toure Ndouo, Fousseyni Samba" sort="Toure Ndouo, Fousseyni Samba" uniqKey="Toure Ndouo F" first="Fousseyni Samba" last="Toure Ndouo">Fousseyni Samba Toure Ndouo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769</s1>
<s2>Franceville</s2>
<s3>GAB</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Gabon</country>
</affiliation>
</author>
<author><name sortKey="Kombila, Maryvonne" sort="Kombila, Maryvonne" uniqKey="Kombila M" first="Maryvonne" last="Kombila">Maryvonne Kombila</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Département de Parasitologie-Mycologie Médecine Tropicale, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009</s1>
<s2>Libreville</s2>
<s3>GAB</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Gabon</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of ethnopharmacology</title>
<title level="j" type="abbreviated">J. ethnopharmacol.</title>
<idno type="ISSN">0378-8741</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
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<seriesStmt><title level="j" type="main">Journal of ethnopharmacology</title>
<title level="j" type="abbreviated">J. ethnopharmacol.</title>
<idno type="ISSN">0378-8741</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antimalarial</term>
<term>Biological activity</term>
<term>Cytotoxicity</term>
<term>Folk medicine</term>
<term>Gabon</term>
<term>In vitro</term>
<term>Medicinal plant</term>
<term>Parasiticide</term>
<term>Pharmacognosy</term>
<term>Plant origin</term>
<term>Plasmodium falciparum</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Activité biologique</term>
<term>In vitro</term>
<term>Antipaludique</term>
<term>Cytotoxicité</term>
<term>Plante médicinale</term>
<term>Médecine traditionnelle</term>
<term>Gabon</term>
<term>Plasmodium falciparum</term>
<term>Pharmacognosie</term>
<term>Origine végétale</term>
<term>Antiparasitaire</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Gabon</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante médicinale</term>
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<front><div type="abstract" xml:lang="en">Aim of the study: As part of a project to identify new compounds active on malarial parasites, we tested the in vitro antiplasmodial activity of nine plants traditionally used to treat malaria symptoms in Haut-Ogooué Province, South-East Gabon. Materials and methods: Dichloromethane and methanolic extracts of each plant were tested for their antiplasmodial activity on two chloroquine-resistant strains of Plasmodium falciparum (FCB and W2), based on lactate dehydrogenase activity. Cytotoxicity was assessed with the MTT test on MRC-5 human diploid embryonic lung cells. Results: The methanolic extract of Staudtia gabonensis and the dichloromethane extract of Adhatoda latibracteata showed high antiplasmodial activity (IC<sub>50</sub>
 < 1 μg/ml) and low cytotoxicity, with selectivity indexes of about 58.25 and 16.43, respectively. The methanolic extract of Monodora myristica and the dichloromethane extract of Afromomum giganteum also showed promising activity (1 < IC<sub>50</sub>
 < 10 μg/ml) and low cytotoxicity, with selectivity indexes about 15.70 and 12.48, respectively. Dichloromethane extracts of Monodora myristica and Leonotis Africana showed moderate activity (10<sub>50</sub>
<40μg/ml), with selectivity indexes about 6.07 and 28.89, respectively. Both extracts of Culcasia lancifolia had IC<sub>50</sub>
 values of 10-40 μg/ml but high cytotoxicity (selectivity indexes <2.77). The methanolic extract of Dorstenia klaineana had moderate antiplasmodial activity (IC<sub>50</sub>
 around 17 μg/ml) but strong cytotoxicity (0.43 μg/ml), giving a selectivity index of about 0.03. Conclusions: Most extracts of nine selected plants traditionally used to treat malaria in Gabon had interesting antiplasmodial activity in vitro. This supports continued investigations of traditional medicines in the search for new antimalarial agents. The compounds responsible for the observed antiplasmodial effects are under investigation.</div>
</front>
</TEI>

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<fA11 i1="01" i2="1"><s1>LEKANA-DOUKI (Jean Bernard)</s1>
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<fA11 i1="02" i2="1"><s1>BONGUI (Jean Bernard)</s1>
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<fA11 i1="03" i2="1"><s1>OYEGUE LIABAGUI (Sandrine Lydie)</s1>
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<fA11 i1="04" i2="1"><s1>EDOU (Sonya Estelle Zang)</s1>
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<fA11 i1="05" i2="1"><s1>ZATRA (Rafika)</s1>
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<fA11 i1="06" i2="1"><s1>BISVIGOU (Ulrich)</s1>
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<fA11 i1="07" i2="1"><s1>DRUILHE (Pierre)</s1>
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<fA11 i1="08" i2="1"><s1>LEBIBI (Jacques)</s1>
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<fA11 i1="09" i2="1"><s1>TOURE NDOUO (Fousseyni Samba)</s1>
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<fA11 i1="10" i2="1"><s1>KOMBILA (Maryvonne)</s1>
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<fA14 i1="01"><s1>Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), B.P. 769</s1>
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<s3>GAB</s3>
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<s2>Franceville</s2>
<s3>GAB</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
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<fA14 i1="03"><s1>Département de Parasitologie-Mycologie Médecine Tropicale, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009</s1>
<s2>Libreville</s2>
<s3>GAB</s3>
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<s2>Libreville</s2>
<s3>GAB</s3>
<sZ>6 aut.</sZ>
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<fA14 i1="05"><s1>Biochemical Parasitology Unit, Institut Pasteur, 25 rue du docteur Roux</s1>
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<sZ>7 aut.</sZ>
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 < 1 μg/ml) and low cytotoxicity, with selectivity indexes of about 58.25 and 16.43, respectively. The methanolic extract of Monodora myristica and the dichloromethane extract of Afromomum giganteum also showed promising activity (1 < IC<sub>50</sub>
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Serveur d'exploration Chloroquine

In vitro antiplasmodial activity and cytotoxicity of nine plants traditionally used in Gabon

In vitro antiplasmodial activity and cytotoxicity of nine plants traditionally used in Gabon

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