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Liposome-mediated transfection of fetal lung epithelial cells : DNA degradation and enhanced superoxide toxicity

Identifieur interne : 000075 ( PascalFrancis/Corpus ); précédent : 000074; suivant : 000076

Liposome-mediated transfection of fetal lung epithelial cells : DNA degradation and enhanced superoxide toxicity

Auteurs : A. K. Tanswell ; O. Staub ; R. Iles ; R. Belcastro ; J. Cabacungan ; L. Sedlackova ; B. Steer ; YANXIA WEN ; J. Hu ; H. O'Brodovich

Source :

RBID : Pascal:98-0466612

Descripteurs français

English descriptors

Abstract

Cationic liposomes, 1:1 (mol/mol) 1,2-dioleoyldimethylammonium chloride-1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, were used to transfect primary cultures of distal rat fetal lung epithelial cells with pCMV4-based plasmids. A DNA-to-lipid ratio of 1:10 to 1:15 (wt/wt) optimized DNA uptake over a 24-h exposure. At a fixed DNA-to-lipid ratio of 1:15, chloramphenicol acetyltransferase (CAT) reporter gene expression declined at lipid concentrations > 2.5 nmol/cm2 cell surface area, whereas DNA uptake remained concentration dependent. CAT expression peaked 48 h after removal of the liposome-DNA complex, declining thereafter. Reporter gene expression was increased, and supercoiled cDNA degradation was reduced by the addition of 0.2 mM nicotinamide and 10 μM chloroquine. Rat fetal lung epithelial cells transfected with two different expression cassettes had an increased susceptibility to superoxide-mediated cytotoxicity. This could be attributed to a nonspecific delivery of exogenous DNA or some other copurified factor. The DNA-dependent increase in superoxide-mediated cytotoxicity, but not basal levels of cytotoxicity, was inhibited by the addition of 0.2 mM nicotinamide and 10 μM chloroquine.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 1040-0605
A03   1    @0 Am. j. physiol., Lung cell. mol. physiol.
A05       @2 19
A06       @2 3
A08 01  1  ENG  @1 Liposome-mediated transfection of fetal lung epithelial cells : DNA degradation and enhanced superoxide toxicity
A11 01  1    @1 TANSWELL (A. K.)
A11 02  1    @1 STAUB (O.)
A11 03  1    @1 ILES (R.)
A11 04  1    @1 BELCASTRO (R.)
A11 05  1    @1 CABACUNGAN (J.)
A11 06  1    @1 SEDLACKOVA (L.)
A11 07  1    @1 STEER (B.)
A11 08  1    @1 YANXIA WEN
A11 09  1    @1 HU (J.)
A11 10  1    @1 O'BRODOVICH (H.)
A14 01      @1 The Medical Research Council Group in Lung Development and Lung Biology Programme, Hospital for Sick Children Research Institute, University of Toronto @2 Toronto, Ontario, M5G 1X8 @3 CAN
A14 02      @1 Divisions of Neonatology and Respiratory Medicine, Department of Paediatrics, University of Toronto @2 Toronto, Ontario, M5G 1X8 @3 CAN
A20       @2 L452-L460
A21       @1 1998
A23 01      @0 ENG
A43 01      @1 INIST @2 22200 @5 354000070307610040
A44       @0 0000 @1 © 1998 INIST-CNRS. All rights reserved.
A45       @0 32 ref.
A47 01  1    @0 98-0466612
A60       @1 P
A61       @0 A
A64   1    @0 American journal of physiology. Lung cellular and molecular physiology
A66 01      @0 USA
C01 01    ENG  @0 Cationic liposomes, 1:1 (mol/mol) 1,2-dioleoyldimethylammonium chloride-1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, were used to transfect primary cultures of distal rat fetal lung epithelial cells with pCMV4-based plasmids. A DNA-to-lipid ratio of 1:10 to 1:15 (wt/wt) optimized DNA uptake over a 24-h exposure. At a fixed DNA-to-lipid ratio of 1:15, chloramphenicol acetyltransferase (CAT) reporter gene expression declined at lipid concentrations > 2.5 nmol/cm2 cell surface area, whereas DNA uptake remained concentration dependent. CAT expression peaked 48 h after removal of the liposome-DNA complex, declining thereafter. Reporter gene expression was increased, and supercoiled cDNA degradation was reduced by the addition of 0.2 mM nicotinamide and 10 μM chloroquine. Rat fetal lung epithelial cells transfected with two different expression cassettes had an increased susceptibility to superoxide-mediated cytotoxicity. This could be attributed to a nonspecific delivery of exogenous DNA or some other copurified factor. The DNA-dependent increase in superoxide-mediated cytotoxicity, but not basal levels of cytotoxicity, was inhibited by the addition of 0.2 mM nicotinamide and 10 μM chloroquine.
C02 01  X    @0 002A31C02A3
C02 02  X    @0 215
C03 01  X  FRE  @0 Poumon @5 01
C03 01  X  ENG  @0 Lung @5 01
C03 01  X  SPA  @0 Pulmón @5 01
C03 02  X  FRE  @0 Foetus @5 02
C03 02  X  ENG  @0 Fetus @5 02
C03 02  X  SPA  @0 Feto @5 02
C03 03  X  FRE  @0 Transfection @5 03
C03 03  X  ENG  @0 Transfection @5 03
C03 03  X  SPA  @0 Transfección @5 03
C03 04  X  FRE  @0 Liposome @5 04
C03 04  X  ENG  @0 Liposome @5 04
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C03 07  X  FRE  @0 Interaction moléculaire @5 08
C03 07  X  ENG  @0 Molecular interaction @5 08
C03 07  X  SPA  @0 Interacción molecular @5 08
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C03 08  X  ENG  @0 Cytotoxicity @5 17
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C03 09  X  FRE  @0 In vitro @5 18
C03 09  X  ENG  @0 In vitro @5 18
C03 09  X  SPA  @0 In vitro @5 18
C03 10  X  FRE  @0 Rat @5 54
C03 10  X  ENG  @0 Rat @5 54
C03 10  X  SPA  @0 Rata @5 54
C03 11  X  FRE  @0 Cellule épithéliale @5 55
C03 11  X  ENG  @0 Epithelial cell @5 55
C03 11  X  SPA  @0 Célula epitelial @5 55
C07 01  X  FRE  @0 Appareil respiratoire @5 20
C07 01  X  ENG  @0 Respiratory system @5 20
C07 01  X  SPA  @0 Aparato respiratorio @5 20
C07 02  X  FRE  @0 Culture cellulaire @5 21
C07 02  X  ENG  @0 Cell culture @5 21
C07 02  X  SPA  @0 Cultivo celular @5 21
C07 03  X  FRE  @0 Hyperoxyde @2 NA @5 22
C07 03  X  ENG  @0 Hyperoxides @2 NA @5 22
C07 03  X  SPA  @0 Hiperóxido @2 NA @5 22
C07 04  X  FRE  @0 Expression génique @5 23
C07 04  X  ENG  @0 Gene expression @5 23
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N21       @1 306

Format Inist (serveur)

NO : PASCAL 98-0466612 INIST
ET : Liposome-mediated transfection of fetal lung epithelial cells : DNA degradation and enhanced superoxide toxicity
AU : TANSWELL (A. K.); STAUB (O.); ILES (R.); BELCASTRO (R.); CABACUNGAN (J.); SEDLACKOVA (L.); STEER (B.); YANXIA WEN; HU (J.); O'BRODOVICH (H.)
AF : The Medical Research Council Group in Lung Development and Lung Biology Programme, Hospital for Sick Children Research Institute, University of Toronto/Toronto, Ontario, M5G 1X8/Canada; Divisions of Neonatology and Respiratory Medicine, Department of Paediatrics, University of Toronto/Toronto, Ontario, M5G 1X8/Canada
DT : Publication en série; Niveau analytique
SO : American journal of physiology. Lung cellular and molecular physiology; ISSN 1040-0605; Etats-Unis; Da. 1998; Vol. 19; No. 3; L452-L460; Bibl. 32 ref.
LA : Anglais
EA : Cationic liposomes, 1:1 (mol/mol) 1,2-dioleoyldimethylammonium chloride-1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, were used to transfect primary cultures of distal rat fetal lung epithelial cells with pCMV4-based plasmids. A DNA-to-lipid ratio of 1:10 to 1:15 (wt/wt) optimized DNA uptake over a 24-h exposure. At a fixed DNA-to-lipid ratio of 1:15, chloramphenicol acetyltransferase (CAT) reporter gene expression declined at lipid concentrations > 2.5 nmol/cm2 cell surface area, whereas DNA uptake remained concentration dependent. CAT expression peaked 48 h after removal of the liposome-DNA complex, declining thereafter. Reporter gene expression was increased, and supercoiled cDNA degradation was reduced by the addition of 0.2 mM nicotinamide and 10 μM chloroquine. Rat fetal lung epithelial cells transfected with two different expression cassettes had an increased susceptibility to superoxide-mediated cytotoxicity. This could be attributed to a nonspecific delivery of exogenous DNA or some other copurified factor. The DNA-dependent increase in superoxide-mediated cytotoxicity, but not basal levels of cytotoxicity, was inhibited by the addition of 0.2 mM nicotinamide and 10 μM chloroquine.
CC : 002A31C02A3; 215
FD : Poumon; Foetus; Transfection; Liposome; Transfert génétique; DNA; Interaction moléculaire; Cytotoxicité; In vitro; Rat; Cellule épithéliale
FG : Appareil respiratoire; Culture cellulaire; Hyperoxyde; Expression génique; Rodentia; Mammalia; Vertebrata
ED : Lung; Fetus; Transfection; Liposome; Genetic transfer; DNA; Molecular interaction; Cytotoxicity; In vitro; Rat; Epithelial cell
EG : Respiratory system; Cell culture; Hyperoxides; Gene expression; Rodentia; Mammalia; Vertebrata
SD : Pulmón; Feto; Transfección; Liposoma; Transferencia genética; DNA; Interacción molecular; Citotoxicidad; In vitro; Rata; Célula epitelial
LO : INIST-22200.354000070307610040
ID : 98-0466612

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Pascal:98-0466612

Le document en format XML

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<fC03 i1="03" i2="X" l="SPA">
<s0>Transfección</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Liposome</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Liposome</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Liposoma</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Transfert génétique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Genetic transfer</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Transferencia genética</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>DNA</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>DNA</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>DNA</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE">
<s0>Interaction moléculaire</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Molecular interaction</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
<s0>Interacción molecular</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE">
<s0>Cytotoxicité</s0>
<s5>17</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG">
<s0>Cytotoxicity</s0>
<s5>17</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
<s0>Citotoxicidad</s0>
<s5>17</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE">
<s0>In vitro</s0>
<s5>18</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG">
<s0>In vitro</s0>
<s5>18</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA">
<s0>In vitro</s0>
<s5>18</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE">
<s0>Rat</s0>
<s5>54</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG">
<s0>Rat</s0>
<s5>54</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA">
<s0>Rata</s0>
<s5>54</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE">
<s0>Cellule épithéliale</s0>
<s5>55</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG">
<s0>Epithelial cell</s0>
<s5>55</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA">
<s0>Célula epitelial</s0>
<s5>55</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Appareil respiratoire</s0>
<s5>20</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Respiratory system</s0>
<s5>20</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Aparato respiratorio</s0>
<s5>20</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Culture cellulaire</s0>
<s5>21</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Cell culture</s0>
<s5>21</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Cultivo celular</s0>
<s5>21</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Hyperoxyde</s0>
<s2>NA</s2>
<s5>22</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Hyperoxides</s0>
<s2>NA</s2>
<s5>22</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Hiperóxido</s0>
<s2>NA</s2>
<s5>22</s5>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Expression génique</s0>
<s5>23</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Gene expression</s0>
<s5>23</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Expresión genética</s0>
<s5>23</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA">
<s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE">
<s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG">
<s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA">
<s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fN21>
<s1>306</s1>
</fN21>
</pA>
</standard>
<server>
<NO>PASCAL 98-0466612 INIST</NO>
<ET>Liposome-mediated transfection of fetal lung epithelial cells : DNA degradation and enhanced superoxide toxicity</ET>
<AU>TANSWELL (A. K.); STAUB (O.); ILES (R.); BELCASTRO (R.); CABACUNGAN (J.); SEDLACKOVA (L.); STEER (B.); YANXIA WEN; HU (J.); O'BRODOVICH (H.)</AU>
<AF>The Medical Research Council Group in Lung Development and Lung Biology Programme, Hospital for Sick Children Research Institute, University of Toronto/Toronto, Ontario, M5G 1X8/Canada; Divisions of Neonatology and Respiratory Medicine, Department of Paediatrics, University of Toronto/Toronto, Ontario, M5G 1X8/Canada</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>American journal of physiology. Lung cellular and molecular physiology; ISSN 1040-0605; Etats-Unis; Da. 1998; Vol. 19; No. 3; L452-L460; Bibl. 32 ref.</SO>
<LA>Anglais</LA>
<EA>Cationic liposomes, 1:1 (mol/mol) 1,2-dioleoyldimethylammonium chloride-1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, were used to transfect primary cultures of distal rat fetal lung epithelial cells with pCMV4-based plasmids. A DNA-to-lipid ratio of 1:10 to 1:15 (wt/wt) optimized DNA uptake over a 24-h exposure. At a fixed DNA-to-lipid ratio of 1:15, chloramphenicol acetyltransferase (CAT) reporter gene expression declined at lipid concentrations > 2.5 nmol/cm
<sup>2</sup>
cell surface area, whereas DNA uptake remained concentration dependent. CAT expression peaked 48 h after removal of the liposome-DNA complex, declining thereafter. Reporter gene expression was increased, and supercoiled cDNA degradation was reduced by the addition of 0.2 mM nicotinamide and 10 μM chloroquine. Rat fetal lung epithelial cells transfected with two different expression cassettes had an increased susceptibility to superoxide-mediated cytotoxicity. This could be attributed to a nonspecific delivery of exogenous DNA or some other copurified factor. The DNA-dependent increase in superoxide-mediated cytotoxicity, but not basal levels of cytotoxicity, was inhibited by the addition of 0.2 mM nicotinamide and 10 μM chloroquine.</EA>
<CC>002A31C02A3; 215</CC>
<FD>Poumon; Foetus; Transfection; Liposome; Transfert génétique; DNA; Interaction moléculaire; Cytotoxicité; In vitro; Rat; Cellule épithéliale</FD>
<FG>Appareil respiratoire; Culture cellulaire; Hyperoxyde; Expression génique; Rodentia; Mammalia; Vertebrata</FG>
<ED>Lung; Fetus; Transfection; Liposome; Genetic transfer; DNA; Molecular interaction; Cytotoxicity; In vitro; Rat; Epithelial cell</ED>
<EG>Respiratory system; Cell culture; Hyperoxides; Gene expression; Rodentia; Mammalia; Vertebrata</EG>
<SD>Pulmón; Feto; Transfección; Liposoma; Transferencia genética; DNA; Interacción molecular; Citotoxicidad; In vitro; Rata; Célula epitelial</SD>
<LO>INIST-22200.354000070307610040</LO>
<ID>98-0466612</ID>
</server>
</inist>
</record>

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