Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection
Identifieur interne : 001030 ( Ncbi/Merge ); précédent : 001029; suivant : 001031Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection
Auteurs : Behnam Dasht Bozorg ; Ali Goodarzi ; Fanak Fahimi ; Payam Tabarsi ; Nahid Shahsavari ; Farzad Kobarfard ; Farzaneh DastanSource :
- Iranian Journal of Pharmaceutical Research : IJPR [ 1735-0328 ] ; 2019.
Abstract
Therapeutic Drug Monitoring (TDM) of first-line anti-tuberculosis (TB) drugs is a decisive tool, allowing the clinician to successfully treat TB patients. The objective of the study was to develop and optimize a simple, sensitive, and reliable high-performance liquid chromatography (HPLC) method for the simultaneous determination of isoniazid (INH), pyrazinamide (PZA), and rifampin (RIF) levels in human plasma. Nicotinamide was used as the internal standard and the samples were prepared after protein precipitation using acetonitrile and zinc sulfate. The separation was achieved using a C18 reversed-phase applying gradient elution. The mobile phase was a combination of water–methanol solution with a ratio of 95:05 (v/v) at the initial phase. All calibration curves had good linearity (r2 > 0.99) and the inter- and intra-day RSDs were lower than 15%. The limit of detection with a signal-to-noise ratio (S/N) of 3:1 was 0.16, 0.5, and 0.33 μg mL–1 for INH, PZA, and RIF, respectively. The method presented here was selective, sensitive, and reproducible, and could be used for therapeutic drug monitoring in the patients who were under treatment with these drugs.
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DOI: 10.22037/ijpr.2019.1100849
PubMed: 32184842
PubMed Central: 7059034
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Fanak" sort="Fahimi, Fanak" uniqKey="Fahimi F" first="Fanak" last="Fahimi">Fanak Fahimi</name><affiliation><nlm:aff id="affa"><italic>Department of Clinical Pharmacy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.</italic></nlm:aff></affiliation><affiliation><nlm:aff id="affb"><italic>Chronic Respiratory Disease Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.</italic></nlm:aff></affiliation></author><author><name sortKey="Tabarsi, Payam" sort="Tabarsi, Payam" uniqKey="Tabarsi P" first="Payam" last="Tabarsi">Payam Tabarsi</name><affiliation><nlm:aff id="affb"><italic>Chronic Respiratory Disease Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.</italic></nlm:aff></affiliation></author><author><name sortKey="Shahsavari, Nahid" sort="Shahsavari, Nahid" 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The objective of the study was to develop and optimize a simple, sensitive, and reliable high-performance liquid chromatography (HPLC) method for the simultaneous determination of isoniazid (INH), pyrazinamide (PZA), and rifampin (RIF) levels in human plasma. Nicotinamide was used as the internal standard and the samples were prepared after protein precipitation using acetonitrile and zinc sulfate. The separation was achieved using a C18 reversed-phase applying gradient elution. The mobile phase was a combination of water–methanol solution with a ratio of 95:05 (v/v) at the initial phase. All calibration curves had good linearity (r<sup>2 </sup>> 0.99) and the inter- and intra-day RSDs were lower than 15%. The limit of detection with a signal-to-noise ratio (S/N) of 3:1 was 0.16, 0.5, and 0.33 μg mL<sup>–1</sup> for INH, PZA, and RIF, respectively. The method presented here was selective, sensitive, and reproducible, and could be used for therapeutic drug monitoring in the patients who were under treatment with these drugs.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kobarfard, F" uniqKey="Kobarfard F">F Kobarfard</name></author><author><name sortKey="Velayati, A" uniqKey="Velayati A">A Velayati</name></author><author><name sortKey="Mosaddegh, M" uniqKey="Mosaddegh M">M Mosaddegh</name></author><author><name sortKey="Massjedi, Mr" uniqKey="Massjedi M">MR Massjedi</name></author><author><name sortKey="Farnia, P" uniqKey="Farnia P">P Farnia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blumberg, Hm" uniqKey="Blumberg H">HM Blumberg</name></author><author><name sortKey="Burman, Wj" uniqKey="Burman W">WJ Burman</name></author><author><name sortKey="Chaisson, Re" uniqKey="Chaisson R">RE Chaisson</name></author><author><name sortKey="Daley, Cl" uniqKey="Daley C">CL Daley</name></author><author><name sortKey="Etkind, Sc" uniqKey="Etkind S">SC Etkind</name></author><author><name sortKey="Friedman, Ln" uniqKey="Friedman L">LN Friedman</name></author><author><name sortKey="Fujiwara, P" uniqKey="Fujiwara P">P Fujiwara</name></author><author><name sortKey="Grzemska, M" uniqKey="Grzemska M">M Grzemska</name></author><author><name sortKey="Hopewell, Pc" uniqKey="Hopewell P">PC Hopewell</name></author><author><name sortKey="Iseman, Md" uniqKey="Iseman M">MD Iseman</name></author><author><name sortKey="Jasmer, Rm" uniqKey="Jasmer R">RM Jasmer</name></author><author><name sortKey="Koppaka, V" uniqKey="Koppaka V">V Koppaka</name></author><author><name sortKey="Menzies, Ri" uniqKey="Menzies R">RI Menzies</name></author><author><name sortKey="O Rien, Rj" uniqKey="O Rien R">RJ O′Brien</name></author><author><name sortKey="Reves, Rr" uniqKey="Reves R">RR Reves</name></author><author><name sortKey="Reichman, Lb" uniqKey="Reichman L">LB Reichman</name></author><author><name sortKey="Simone, Pm" uniqKey="Simone P">PM Simone</name></author><author><name sortKey="Starke, Jr" uniqKey="Starke J">JR Starke</name></author><author><name sortKey="Vernon, Aa" uniqKey="Vernon A">AA Vernon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ducati, Rg" uniqKey="Ducati R">RG Ducati</name></author><author><name sortKey="Ruffino Netto, A" uniqKey="Ruffino Netto A">A Ruffino-Netto</name></author><author><name sortKey="Basso, La" uniqKey="Basso L">LA Basso</name></author><author><name sortKey="Santos, Ds" uniqKey="Santos D">DS Santos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peloquin, Ca" uniqKey="Peloquin C">CA Peloquin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dastan, F" uniqKey="Dastan F">F Dastan</name></author><author><name sortKey="Bozorg, Bd" uniqKey="Bozorg B">BD Bozorg</name></author><author><name sortKey="Goodarzi, A" uniqKey="Goodarzi A">A Goodarzi</name></author><author><name sortKey="Salamzadeh, J" uniqKey="Salamzadeh J">J Salamzadeh</name></author><author><name sortKey="Tabarsi, P" uniqKey="Tabarsi P">P Tabarsi</name></author><author><name sortKey="Kobarfard, F" uniqKey="Kobarfard F">F Kobarfard</name></author><author><name sortKey="Fahimi, F" uniqKey="Fahimi F">F Fahimi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiner, M" uniqKey="Weiner M">M Weiner</name></author><author><name sortKey="Khan, A" uniqKey="Khan A">A Khan</name></author><author><name sortKey="Benator, D" uniqKey="Benator D">D Benator</name></author><author><name sortKey="Peloquin, Ca" uniqKey="Peloquin C">CA Peloquin</name></author><author><name sortKey="Burman, W" uniqKey="Burman W">W Burman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peloquin, Ca" uniqKey="Peloquin C">CA Peloquin</name></author><author><name sortKey="Benator, D" uniqKey="Benator D">D Benator</name></author><author><name sortKey="Hayden, K" uniqKey="Hayden K">K Hayden</name></author><author><name sortKey="Pizzano, D" uniqKey="Pizzano D">D Pizzano</name></author><author><name sortKey="Tb, Trials Consortium" uniqKey="Tb T">Trials Consortium TB</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Um, Sw" uniqKey="Um S">SW Um</name></author><author><name sortKey="Lee, Sw" uniqKey="Lee S">SW Lee</name></author><author><name sortKey="Kwon, Sy" uniqKey="Kwon S">SY Kwon</name></author><author><name sortKey="Yoon, Hi" uniqKey="Yoon H">HI Yoon</name></author><author><name sortKey="Park, Ku" uniqKey="Park K">KU Park</name></author><author><name sortKey="Song, J" uniqKey="Song J">J Song</name></author><author><name sortKey="Lee, Ct" uniqKey="Lee C">CT Lee</name></author><author><name sortKey="Lee, Jh" uniqKey="Lee J">JH Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiner, M" uniqKey="Weiner M">M Weiner</name></author><author><name sortKey="Burman, W" uniqKey="Burman W">W Burman</name></author><author><name sortKey="Vernon, A" uniqKey="Vernon A">A Vernon</name></author><author><name sortKey="Benator, D" uniqKey="Benator D">D Benator</name></author><author><name sortKey="Peloquin, Ca" uniqKey="Peloquin C">CA Peloquin</name></author><author><name sortKey="Khan, A" uniqKey="Khan A">A Khan</name></author><author><name sortKey="Weis, S" uniqKey="Weis S">S Weis</name></author><author><name sortKey="King, B" uniqKey="King B">B King</name></author><author><name sortKey="Shah, N" uniqKey="Shah N">N Shah</name></author><author><name sortKey="Hodge, T" uniqKey="Hodge T">T Hodge</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Unsalan, S" uniqKey="Unsalan S">S Unsalan</name></author><author><name sortKey="Sancar, M" uniqKey="Sancar M">M Sancar</name></author><author><name sortKey="Bekce, B" uniqKey="Bekce B">B Bekce</name></author><author><name sortKey="Clark, Pm" uniqKey="Clark P">PM Clark</name></author><author><name sortKey="Karagoz, T" uniqKey="Karagoz T">T Karagoz</name></author><author><name sortKey="Izzettin, Fv" uniqKey="Izzettin F">FV Izzettin</name></author><author><name sortKey="Rollas, S" uniqKey="Rollas S">S Rollas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z Zhou</name></author><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Liu, P" uniqKey="Liu P">P Liu</name></author><author><name sortKey="Shen, M" uniqKey="Shen M">M Shen</name></author><author><name sortKey="Zou, F" uniqKey="Zou F">F Zou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, Js" uniqKey="Park J">JS Park</name></author><author><name sortKey="Lee, Jy" uniqKey="Lee J">JY Lee</name></author><author><name sortKey="Lee, Yj" uniqKey="Lee Y">YJ Lee</name></author><author><name sortKey="Kim, Sj" uniqKey="Kim S">SJ Kim</name></author><author><name sortKey="Cho, Yj" uniqKey="Cho Y">YJ Cho</name></author><author><name sortKey="Yoon, H I" uniqKey="Yoon H">H I Yoon</name></author><author><name sortKey="Lee, Ct" uniqKey="Lee C">CT Lee</name></author><author><name sortKey="Song, J" uniqKey="Song J">J Song</name></author><author><name sortKey="Lee, Jh" uniqKey="Lee J">JH Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Le, Guellec C" uniqKey="Le G">Guellec C Le</name></author><author><name sortKey="Gaudet, Ml" uniqKey="Gaudet M">ML Gaudet</name></author><author><name sortKey="Lamanetre, S" uniqKey="Lamanetre S">S Lamanetre</name></author><author><name sortKey="Breteau, M" uniqKey="Breteau M">M Breteau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fahimi, F" uniqKey="Fahimi F">F Fahimi</name></author><author><name sortKey="Tabarsi, P" uniqKey="Tabarsi P">P Tabarsi</name></author><author><name sortKey="Kobarfard, F" uniqKey="Kobarfard F">F Kobarfard</name></author><author><name sortKey="Bozorg, Bd" uniqKey="Bozorg B">BD Bozorg</name></author><author><name sortKey="Goodarzi, A" uniqKey="Goodarzi A">A Goodarzi</name></author><author><name sortKey="Dastan, F" uniqKey="Dastan F">F Dastan</name></author><author><name sortKey="Shahsavari, N" uniqKey="Shahsavari N">N Shahsavari</name></author><author><name sortKey="Emami, S" uniqKey="Emami S">S Emami</name></author><author><name sortKey="Habibi, M" uniqKey="Habibi M">M Habibi</name></author><author><name sortKey="Salamzadeh, J" uniqKey="Salamzadeh J">J Salamzadeh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khalili, H" uniqKey="Khalili H">H Khalili</name></author><author><name sortKey="Dastan, F" uniqKey="Dastan F">F Dastan</name></author><author><name sortKey="Dehghan, Manshadi Sa" uniqKey="Dehghan M">Manshadi SA Dehghan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dastan, F" uniqKey="Dastan F">F Dastan</name></author><author><name sortKey="Hajhossein, Talasaz A" uniqKey="Hajhossein T">Talasaz A Hajhossein</name></author><author><name sortKey="Mojtahedzadeh, M" uniqKey="Mojtahedzadeh M">M Mojtahedzadeh</name></author><author><name sortKey="Karimi, A" uniqKey="Karimi A">A Karimi</name></author><author><name sortKey="Salehiomran, A" uniqKey="Salehiomran A">A Salehiomran</name></author><author><name sortKey="Bina, P" uniqKey="Bina P">P Bina</name></author><author><name sortKey="Jalili, A" uniqKey="Jalili A">A Jalili</name></author><author><name sortKey="Aghaie, Z" uniqKey="Aghaie Z">Z Aghaie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schellinger, Ap" uniqKey="Schellinger A">AP Schellinger</name></author><author><name sortKey="Stoll, Dr" uniqKey="Stoll D">DR Stoll</name></author><author><name sortKey="Carr, Pw" uniqKey="Carr P">PW Carr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ladda, Pl" uniqKey="Ladda P">PL Ladda</name></author><author><name sortKey="Magdum, Cs" uniqKey="Magdum C">CS Magdum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ghazizadeh, F" uniqKey="Ghazizadeh F">F Ghazizadeh</name></author><author><name sortKey="Ghaffari, S" uniqKey="Ghaffari S">S Ghaffari</name></author><author><name sortKey="Mirshojaei, Sf" uniqKey="Mirshojaei S">SF Mirshojaei</name></author><author><name sortKey="Mazidid, M" uniqKey="Mazidid M">M Mazidid</name></author><author><name sortKey="Azarmi, S" uniqKey="Azarmi S">S Azarmi</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Iran J Pharm Res</journal-id><journal-id journal-id-type="iso-abbrev">Iran J Pharm Res</journal-id><journal-id journal-id-type="publisher-id">IJPR</journal-id><journal-title-group><journal-title>Iranian Journal of Pharmaceutical Research : IJPR</journal-title></journal-title-group><issn pub-type="ppub">1735-0328</issn><issn pub-type="epub">1726-6890</issn><publisher><publisher-name>Shaheed Beheshti University of Medical Sciences</publisher-name><publisher-loc>Tehran, Iran</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32184842</article-id><article-id pub-id-type="pmc">7059034</article-id><article-id pub-id-type="doi">10.22037/ijpr.2019.1100849</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dasht Bozorg</surname><given-names>Behnam</given-names></name><xref ref-type="aff" rid="affa">a</xref></contrib><contrib contrib-type="author"><name><surname>Goodarzi</surname><given-names>Ali</given-names></name><xref ref-type="aff" rid="affa">a</xref></contrib><contrib contrib-type="author"><name><surname>Fahimi</surname><given-names>Fanak</given-names></name><xref ref-type="aff" rid="affa">a</xref><xref ref-type="aff" rid="affb">b</xref></contrib><contrib contrib-type="author"><name><surname>Tabarsi</surname><given-names>Payam</given-names></name><xref ref-type="aff" rid="affb">b</xref></contrib><contrib contrib-type="author"><name><surname>Shahsavari</surname><given-names>Nahid</given-names></name><xref ref-type="aff" rid="affa">a</xref></contrib><contrib contrib-type="author"><name><surname>Kobarfard</surname><given-names>Farzad</given-names></name><xref ref-type="aff" rid="affc">c</xref></contrib><contrib contrib-type="author"><name><surname>Dastan</surname><given-names>Farzaneh</given-names></name><xref ref-type="aff" rid="affa">a</xref><xref ref-type="aff" rid="affb">b</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="affa"><label>a</label><italic>Department of Clinical Pharmacy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.</italic></aff><aff id="affb"><label>b</label><italic>Chronic Respiratory Disease Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.</italic></aff><aff id="affc"><label>c</label><italic>Phytochemistry Research Center, Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.</italic></aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author: E-mail: fzh.dastan@gmail.com</corresp></author-notes><pub-date pub-type="ppub"><season>Autumn</season><year>2019</year></pub-date><volume>18</volume><issue>4</issue><fpage>1735</fpage><lpage>1741</lpage><history><date date-type="received"><month>6</month><year>2018</year></date><date date-type="accepted"><month>2</month><year>2019</year></date></history><permissions><license license-type="open-access"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">http://creativecommons.org/licenses/by/3.0/</ext-link>) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>Therapeutic Drug Monitoring (TDM) of first-line anti-tuberculosis (TB) drugs is a decisive tool, allowing the clinician to successfully treat TB patients. The objective of the study was to develop and optimize a simple, sensitive, and reliable high-performance liquid chromatography (HPLC) method for the simultaneous determination of isoniazid (INH), pyrazinamide (PZA), and rifampin (RIF) levels in human plasma. Nicotinamide was used as the internal standard and the samples were prepared after protein precipitation using acetonitrile and zinc sulfate. The separation was achieved using a C18 reversed-phase applying gradient elution. The mobile phase was a combination of water–methanol solution with a ratio of 95:05 (v/v) at the initial phase. All calibration curves had good linearity (r<sup>2 </sup>> 0.99) and the inter- and intra-day RSDs were lower than 15%. The limit of detection with a signal-to-noise ratio (S/N) of 3:1 was 0.16, 0.5, and 0.33 μg mL<sup>–1</sup> for INH, PZA, and RIF, respectively. The method presented here was selective, sensitive, and reproducible, and could be used for therapeutic drug monitoring in the patients who were under treatment with these drugs.</p></abstract><kwd-group><title>Key Words</title><kwd>Anti-TB drugs</kwd><kwd>Therapeutic drug monitoring</kwd><kwd>HPLC-UV</kwd><kwd>Isoniazid</kwd><kwd>Pyrazinamide</kwd><kwd>Rifampin</kwd></kwd-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Dasht Bozorg, Behnam" sort="Dasht Bozorg, Behnam" uniqKey="Dasht Bozorg B" first="Behnam" last="Dasht Bozorg">Behnam Dasht Bozorg</name><name sortKey="Dastan, Farzaneh" sort="Dastan, Farzaneh" uniqKey="Dastan F" first="Farzaneh" last="Dastan">Farzaneh Dastan</name><name sortKey="Fahimi, Fanak" sort="Fahimi, Fanak" uniqKey="Fahimi F" first="Fanak" last="Fahimi">Fanak Fahimi</name><name sortKey="Goodarzi, Ali" sort="Goodarzi, Ali" uniqKey="Goodarzi A" first="Ali" last="Goodarzi">Ali Goodarzi</name><name sortKey="Kobarfard, Farzad" sort="Kobarfard, Farzad" uniqKey="Kobarfard F" first="Farzad" last="Kobarfard">Farzad Kobarfard</name><name sortKey="Shahsavari, Nahid" sort="Shahsavari, Nahid" uniqKey="Shahsavari N" first="Nahid" last="Shahsavari">Nahid Shahsavari</name><name sortKey="Tabarsi, Payam" sort="Tabarsi, Payam" uniqKey="Tabarsi P" first="Payam" last="Tabarsi">Payam Tabarsi</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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