Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication
Identifieur interne : 000E78 ( Ncbi/Merge ); précédent : 000E77; suivant : 000E79Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication
Auteurs : Yuka Horio [Japon] ; Riho Sogabe [Japon] ; Mototada Shichiri [Japon] ; Noriko Ishida [Japon] ; Ryosuke Morimoto [Japon] ; Atsushi Ohshima [Japon] ; Yuji Isegawa [Japon]Source :
- Journal of Clinical Biochemistry and Nutrition [ 0912-0009 ] ; 2020.
Abstract
This study was conducted to evaluate the regulation mechanism of influenza virus replication following treatment of Madin-Darby canine kidney cells with the soy isoflavone daidzein. We performed comparative qualitative and quantitative analyses of lipid peroxide between mock-infected and virus-infected cells treated with or without daidzein, as it had been reported that daidzein was an antioxidant and lipid peroxide levels increased upon virus infection. Contrary to our belief, lipid peroxides were not elevated in virus-infected cells and no decrease in lipid peroxides was observed in daidzein-treated cells. In daidzein-treated cells, 5-hydroxyeicosatetraenoic acid, the 5-lipoxygenase product derived from arachidonate, was significantly elevated compared to other lipid peroxides. Zileuton (5-lipoxygenase inhibitor) and 5-lipoxygenase knockdown reduced the daidzein-induced antiviral effect. Moreover, virus replication was regulated by treatment with 5-hydroperoxyeicosatetraenoic acid, a precursor of 5-hydroxyeicosatetraenoic acid and 5-lipoxygenase primary product. These results suggest that daidzein regulates virus replication via signal transduction through 5-lipoxygenase products.
Url:
DOI: 10.3164/jcbn.19-70
PubMed: 32001954
PubMed Central: 6983437
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000662
- to stream Pmc, to step Curation: 000662
- to stream Pmc, to step Checkpoint: 000121
Links to Exploration step
PMC:6983437Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication</title><author><name sortKey="Horio, Yuka" sort="Horio, Yuka" uniqKey="Horio Y" first="Yuka" last="Horio">Yuka Horio</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author><author><name sortKey="Sogabe, Riho" sort="Sogabe, Riho" uniqKey="Sogabe R" first="Riho" last="Sogabe">Riho Sogabe</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author><author><name sortKey="Shichiri, Mototada" sort="Shichiri, Mototada" uniqKey="Shichiri M" first="Mototada" last="Shichiri">Mototada Shichiri</name><affiliation wicri:level="1"><nlm:aff id="aff2">Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577</wicri:regionArea><wicri:noRegion>Osaka 563-8577</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), 1-1-1 Higashi, Tsukuba-shi, Ibaraki 305-8562, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), 1-1-1 Higashi, Tsukuba-shi, Ibaraki 305-8562</wicri:regionArea><wicri:noRegion>Ibaraki 305-8562</wicri:noRegion></affiliation></author><author><name sortKey="Ishida, Noriko" sort="Ishida, Noriko" uniqKey="Ishida N" first="Noriko" last="Ishida">Noriko Ishida</name><affiliation wicri:level="1"><nlm:aff id="aff2">Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577</wicri:regionArea><wicri:noRegion>Osaka 563-8577</wicri:noRegion></affiliation></author><author><name sortKey="Morimoto, Ryosuke" sort="Morimoto, Ryosuke" uniqKey="Morimoto R" first="Ryosuke" last="Morimoto">Ryosuke Morimoto</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author><author><name sortKey="Ohshima, Atsushi" sort="Ohshima, Atsushi" uniqKey="Ohshima A" first="Atsushi" last="Ohshima">Atsushi Ohshima</name><affiliation wicri:level="1"><nlm:aff id="aff4">Genomics Program, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama, Shiga 526-0829, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Genomics Program, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama, Shiga 526-0829</wicri:regionArea><wicri:noRegion>Shiga 526-0829</wicri:noRegion></affiliation></author><author><name sortKey="Isegawa, Yuji" sort="Isegawa, Yuji" uniqKey="Isegawa Y" first="Yuji" last="Isegawa">Yuji Isegawa</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Institute for Biosciences, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Biosciences, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32001954</idno><idno type="pmc">6983437</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983437</idno><idno type="RBID">PMC:6983437</idno><idno type="doi">10.3164/jcbn.19-70</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000662</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000662</idno><idno type="wicri:Area/Pmc/Curation">000662</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000662</idno><idno type="wicri:Area/Pmc/Checkpoint">000121</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000121</idno><idno type="wicri:Area/Ncbi/Merge">000E78</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication</title><author><name sortKey="Horio, Yuka" sort="Horio, Yuka" uniqKey="Horio Y" first="Yuka" last="Horio">Yuka Horio</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author><author><name sortKey="Sogabe, Riho" sort="Sogabe, Riho" uniqKey="Sogabe R" first="Riho" last="Sogabe">Riho Sogabe</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author><author><name sortKey="Shichiri, Mototada" sort="Shichiri, Mototada" uniqKey="Shichiri M" first="Mototada" last="Shichiri">Mototada Shichiri</name><affiliation wicri:level="1"><nlm:aff id="aff2">Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577</wicri:regionArea><wicri:noRegion>Osaka 563-8577</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), 1-1-1 Higashi, Tsukuba-shi, Ibaraki 305-8562, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), 1-1-1 Higashi, Tsukuba-shi, Ibaraki 305-8562</wicri:regionArea><wicri:noRegion>Ibaraki 305-8562</wicri:noRegion></affiliation></author><author><name sortKey="Ishida, Noriko" sort="Ishida, Noriko" uniqKey="Ishida N" first="Noriko" last="Ishida">Noriko Ishida</name><affiliation wicri:level="1"><nlm:aff id="aff2">Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577</wicri:regionArea><wicri:noRegion>Osaka 563-8577</wicri:noRegion></affiliation></author><author><name sortKey="Morimoto, Ryosuke" sort="Morimoto, Ryosuke" uniqKey="Morimoto R" first="Ryosuke" last="Morimoto">Ryosuke Morimoto</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author><author><name sortKey="Ohshima, Atsushi" sort="Ohshima, Atsushi" uniqKey="Ohshima A" first="Atsushi" last="Ohshima">Atsushi Ohshima</name><affiliation wicri:level="1"><nlm:aff id="aff4">Genomics Program, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama, Shiga 526-0829, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Genomics Program, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama, Shiga 526-0829</wicri:regionArea><wicri:noRegion>Shiga 526-0829</wicri:noRegion></affiliation></author><author><name sortKey="Isegawa, Yuji" sort="Isegawa, Yuji" uniqKey="Isegawa Y" first="Yuji" last="Isegawa">Yuji Isegawa</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Institute for Biosciences, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Biosciences, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558</wicri:regionArea><wicri:noRegion>Hyogo 663-8558</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Clinical Biochemistry and Nutrition</title><idno type="ISSN">0912-0009</idno><idno type="eISSN">1880-5086</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>This study was conducted to evaluate the regulation mechanism of influenza virus replication following treatment of Madin-Darby canine kidney cells with the soy isoflavone daidzein. We performed comparative qualitative and quantitative analyses of lipid peroxide between mock-infected and virus-infected cells treated with or without daidzein, as it had been reported that daidzein was an antioxidant and lipid peroxide levels increased upon virus infection. Contrary to our belief, lipid peroxides were not elevated in virus-infected cells and no decrease in lipid peroxides was observed in daidzein-treated cells. In daidzein-treated cells, 5-hydroxyeicosatetraenoic acid, the 5-lipoxygenase product derived from arachidonate, was significantly elevated compared to other lipid peroxides. Zileuton (5-lipoxygenase inhibitor) and 5-lipoxygenase knockdown reduced the daidzein-induced antiviral effect. Moreover, virus replication was regulated by treatment with 5-hydroperoxyeicosatetraenoic acid, a precursor of 5-hydroxyeicosatetraenoic acid and 5-lipoxygenase primary product. These results suggest that daidzein regulates virus replication via signal transduction through 5-lipoxygenase products.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Belser, Ja" uniqKey="Belser J">JA Belser</name></author><author><name sortKey="Maines, Tr" uniqKey="Maines T">TR Maines</name></author><author><name sortKey="Tumpey, Tm" uniqKey="Tumpey T">TM Tumpey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chow, Ej" uniqKey="Chow E">EJ Chow</name></author><author><name sortKey="Doyle, Jd" uniqKey="Doyle J">JD Doyle</name></author><author><name sortKey="Uyeki, Tm" uniqKey="Uyeki T">TM Uyeki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Le, Qm" uniqKey="Le Q">QM Le</name></author><author><name sortKey="Kiso, M" uniqKey="Kiso M">M Kiso</name></author><author><name sortKey="Someya, K" uniqKey="Someya K">K Someya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bloom, Jd" uniqKey="Bloom J">JD Bloom</name></author><author><name sortKey="Gong, Li" uniqKey="Gong L">LI Gong</name></author><author><name sortKey="Baltimore, D" uniqKey="Baltimore D">D Baltimore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Furuta, Y" uniqKey="Furuta Y">Y Furuta</name></author><author><name sortKey="Takahashi, K" uniqKey="Takahashi K">K Takahashi</name></author><author><name sortKey="Fukuda, Y" uniqKey="Fukuda Y">Y Fukuda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kiso, M" uniqKey="Kiso M">M Kiso</name></author><author><name sortKey="Takahashi, K" uniqKey="Takahashi K">K Takahashi</name></author><author><name sortKey="Sakai Tagawa, Y" uniqKey="Sakai Tagawa Y">Y Sakai-Tagawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagai, E" uniqKey="Nagai E">E Nagai</name></author><author><name sortKey="Iwai, M" uniqKey="Iwai M">M Iwai</name></author><author><name sortKey="Koketsu, R" uniqKey="Koketsu R">R Koketsu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Christen, S" uniqKey="Christen S">S Christen</name></author><author><name sortKey="Peterhans, E" uniqKey="Peterhans E">E Peterhans</name></author><author><name sortKey="Stocker, R" uniqKey="Stocker R">R Stocker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jacoby, Db" uniqKey="Jacoby D">DB Jacoby</name></author><author><name sortKey="Choi, Am" uniqKey="Choi A">AM Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Akaike, T" uniqKey="Akaike T">T Akaike</name></author><author><name sortKey="Noguchi, Y" uniqKey="Noguchi Y">Y Noguchi</name></author><author><name sortKey="Ijiri, S" uniqKey="Ijiri S">S Ijiri</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Jung, Ki" uniqKey="Jung K">KI Jung</name></author><author><name sortKey="Pyo, Cw" uniqKey="Pyo C">CW Pyo</name></author><author><name sortKey="Choi, Sy" uniqKey="Choi S">SY Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Franke, Aa" uniqKey="Franke A">AA Franke</name></author><author><name sortKey="Lai, Jf" uniqKey="Lai J">JF Lai</name></author><author><name sortKey="Halm, Bm" uniqKey="Halm B">BM Halm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sankar, P" uniqKey="Sankar P">P Sankar</name></author><author><name sortKey="Zachariah, B" uniqKey="Zachariah B">B Zachariah</name></author><author><name sortKey="Vickneshwaran, V" uniqKey="Vickneshwaran V">V Vickneshwaran</name></author><author><name sortKey="Jacob, Se" uniqKey="Jacob S">SE Jacob</name></author><author><name sortKey="Sridhar, Mg" uniqKey="Sridhar M">MG Sridhar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name></author><author><name sortKey="Ma, X" uniqKey="Ma X">X Ma</name></author><author><name sortKey="Lin, Y" uniqKey="Lin Y">Y Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, M" uniqKey="Jin M">M Jin</name></author><author><name sortKey="Shen, Mh" uniqKey="Shen M">MH Shen</name></author><author><name sortKey="Jin, Mh" uniqKey="Jin M">MH Jin</name></author><author><name sortKey="Jin, Ah" uniqKey="Jin A">AH Jin</name></author><author><name sortKey="Yin, Xz" uniqKey="Yin X">XZ Yin</name></author><author><name sortKey="Quan, Js" uniqKey="Quan J">JS Quan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuiper, Gg" uniqKey="Kuiper G">GG Kuiper</name></author><author><name sortKey="Carlsson, B" uniqKey="Carlsson B">B Carlsson</name></author><author><name sortKey="Grandien, K" uniqKey="Grandien K">K Grandien</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuiper, Gg" uniqKey="Kuiper G">GG Kuiper</name></author><author><name sortKey="Lemmen, Jg" uniqKey="Lemmen J">JG Lemmen</name></author><author><name sortKey="Carlsson, B" uniqKey="Carlsson B">B Carlsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uif Lean, A" uniqKey="Uif Lean A">A Uifälean</name></author><author><name sortKey="Schneider, S" uniqKey="Schneider S">S Schneider</name></author><author><name sortKey="Ionescu, C" uniqKey="Ionescu C">C Ionescu</name></author><author><name sortKey="Lalk, M" uniqKey="Lalk M">M Lalk</name></author><author><name sortKey="Iuga, Ca" uniqKey="Iuga C">CA Iuga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Folman, Y" uniqKey="Folman Y">Y Folman</name></author><author><name sortKey="Pope, Gs" uniqKey="Pope G">GS Pope</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yan, Gr" uniqKey="Yan G">GR Yan</name></author><author><name sortKey="Xiao, Cl" uniqKey="Xiao C">CL Xiao</name></author><author><name sortKey="He, Gw" uniqKey="He G">GW He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hafez, Hs" uniqKey="Hafez H">HS Hafez</name></author><author><name sortKey="Ghareeb, Da" uniqKey="Ghareeb D">DA Ghareeb</name></author><author><name sortKey="Saleh, Sr" uniqKey="Saleh S">SR Saleh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Ms" uniqKey="Kim M">MS Kim</name></author><author><name sortKey="Kim, B" uniqKey="Kim B">B Kim</name></author><author><name sortKey="Park, H" uniqKey="Park H">H Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoshida, Y" uniqKey="Yoshida Y">Y Yoshida</name></author><author><name sortKey="Kodai, S" uniqKey="Kodai S">S Kodai</name></author><author><name sortKey="Takemura, S" uniqKey="Takemura S">S Takemura</name></author><author><name sortKey="Minamiyama, Y" uniqKey="Minamiyama Y">Y Minamiyama</name></author><author><name sortKey="Niki, E" uniqKey="Niki E">E Niki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Umeno, A" uniqKey="Umeno A">A Umeno</name></author><author><name sortKey="Shichiri, M" uniqKey="Shichiri M">M Shichiri</name></author><author><name sortKey="Ishida, N" uniqKey="Ishida N">N Ishida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shichiri, M" uniqKey="Shichiri M">M Shichiri</name></author><author><name sortKey="Ishida, N" uniqKey="Ishida N">N Ishida</name></author><author><name sortKey="Hagihara, Y" uniqKey="Hagihara Y">Y Hagihara</name></author><author><name sortKey="Yoshida, Y" uniqKey="Yoshida Y">Y Yoshida</name></author><author><name sortKey="Kume, A" uniqKey="Kume A">A Kume</name></author><author><name sortKey="Suzuki, H" uniqKey="Suzuki H">H Suzuki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lisovyy, Oo" uniqKey="Lisovyy O">OO Lisovyy</name></author><author><name sortKey="Dosenko, Ve" uniqKey="Dosenko V">VE Dosenko</name></author><author><name sortKey="Nagibin, Vs" uniqKey="Nagibin V">VS Nagibin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shichiri, M" uniqKey="Shichiri M">M Shichiri</name></author><author><name sortKey="Kono, N" uniqKey="Kono N">N Kono</name></author><author><name sortKey="Shimanaka, Y" uniqKey="Shimanaka Y">Y Shimanaka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steinhilber, D" uniqKey="Steinhilber D">D Steinhilber</name></author><author><name sortKey="Fischer, As" uniqKey="Fischer A">AS Fischer</name></author><author><name sortKey="Metzner, J" uniqKey="Metzner J">J Metzner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, H" uniqKey="Liu H">H Liu</name></author><author><name sortKey="Zhong, H" uniqKey="Zhong H">H Zhong</name></author><author><name sortKey="Leng, L" uniqKey="Leng L">L Leng</name></author><author><name sortKey="Jiang, Z" uniqKey="Jiang Z">Z Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="B Ck, M" uniqKey="B Ck M">M Bäck</name></author><author><name sortKey="Yurdagul, A" uniqKey="Yurdagul A">A Yurdagul</name></author><author><name sortKey="Tabas, I" uniqKey="Tabas I">I Tabas</name></author><author><name sortKey="Oorni, K" uniqKey="Oorni K">K Öörni</name></author><author><name sortKey="Kovanen, Pt" uniqKey="Kovanen P">PT Kovanen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Serhan, Cn" uniqKey="Serhan C">CN Serhan</name></author><author><name sortKey="Dalli, J" uniqKey="Dalli J">J Dalli</name></author><author><name sortKey="Colas, Ra" uniqKey="Colas R">RA Colas</name></author><author><name sortKey="Winkler, Jw" uniqKey="Winkler J">JW Winkler</name></author><author><name sortKey="Chiang, N" uniqKey="Chiang N">N Chiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morita, M" uniqKey="Morita M">M Morita</name></author><author><name sortKey="Kuba, K" uniqKey="Kuba K">K Kuba</name></author><author><name sortKey="Ichikawa, A" uniqKey="Ichikawa A">A Ichikawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="R Dmark, O" uniqKey="R Dmark O">O Rådmark</name></author><author><name sortKey="Werz, O" uniqKey="Werz O">O Werz</name></author><author><name sortKey="Steinhilber, D" uniqKey="Steinhilber D">D Steinhilber</name></author><author><name sortKey="Samuelsson, B" uniqKey="Samuelsson B">B Samuelsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Recchiuti, A" uniqKey="Recchiuti A">A Recchiuti</name></author><author><name sortKey="Serhan, Cn" uniqKey="Serhan C">CN Serhan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="King, Ra" uniqKey="King R">RA King</name></author><author><name sortKey="Bursill, Db" uniqKey="Bursill D">DB Bursill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, Wc" uniqKey="Cheng W">WC Cheng</name></author><author><name sortKey="Lo, Sc" uniqKey="Lo S">SC Lo</name></author><author><name sortKey="Tsai, Ks" uniqKey="Tsai K">KS Tsai</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Biochem Nutr</journal-id><journal-id journal-id-type="iso-abbrev">J Clin Biochem Nutr</journal-id><journal-id journal-id-type="publisher-id">JCBN</journal-id><journal-title-group><journal-title>Journal of Clinical Biochemistry and Nutrition</journal-title></journal-title-group><issn pub-type="ppub">0912-0009</issn><issn pub-type="epub">1880-5086</issn><publisher><publisher-name>the Society for Free Radical Research Japan</publisher-name><publisher-loc>Kyoto, Japan</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32001954</article-id><article-id pub-id-type="pmc">6983437</article-id><article-id pub-id-type="publisher-id">DN/JST.JSTAGE/jcbn/19-70</article-id><article-id pub-id-type="doi">10.3164/jcbn.19-70</article-id><article-id pub-id-type="publisher-manuscript">19-70</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Horio</surname><given-names>Yuka</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="author-notes" rid="fn1">†</xref></contrib><contrib contrib-type="author"><name><surname>Sogabe</surname><given-names>Riho</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="author-notes" rid="fn1">†</xref></contrib><contrib contrib-type="author"><name><surname>Shichiri</surname><given-names>Mototada</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref><xref ref-type="author-notes" rid="fn1">†</xref></contrib><contrib contrib-type="author"><name><surname>Ishida</surname><given-names>Noriko</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Morimoto</surname><given-names>Ryosuke</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ohshima</surname><given-names>Atsushi</given-names></name><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Isegawa</surname><given-names>Yuji</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff5">5</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="aff1"><label>1</label>Department of Food Sciences and Nutrition, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</aff><aff id="aff2"><label>2</label>Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan</aff><aff id="aff3"><label>3</label>DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), 1-1-1 Higashi, Tsukuba-shi, Ibaraki 305-8562, Japan</aff><aff id="aff4"><label>4</label>Genomics Program, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama, Shiga 526-0829, Japan</aff><aff id="aff5"><label>5</label>Institute for Biosciences, Mukogawa Women’s University, 6-46 Ikebiraki, Nishinomiya, Hyogo 663-8558, Japan</aff></contrib-group><author-notes><fn id="fn1"><label>†</label><p>These authors contributed equally: YH, RS, MS.</p></fn><corresp id="cor1">*To whom correspondence should be addressed. E-mail: <email>isegawa@mukogawa-u.ac.jp</email></corresp></author-notes><pub-date pub-type="ppub"><month>1</month><year>2020</year></pub-date><pub-date pub-type="epub"><day>1</day><month>1</month><year>2020</year></pub-date><volume>66</volume><issue>1</issue><fpage>36</fpage><lpage>42</lpage><history><date date-type="received"><day>17</day><month>8</month><year>2019</year></date><date date-type="accepted"><day>29</day><month>9</month><year>2019</year></date></history><permissions><copyright-statement>Copyright © 2020 JCBN</copyright-statement><copyright-year>2020</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/"><license-p>This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>This study was conducted to evaluate the regulation mechanism of influenza virus replication following treatment of Madin-Darby canine kidney cells with the soy isoflavone daidzein. We performed comparative qualitative and quantitative analyses of lipid peroxide between mock-infected and virus-infected cells treated with or without daidzein, as it had been reported that daidzein was an antioxidant and lipid peroxide levels increased upon virus infection. Contrary to our belief, lipid peroxides were not elevated in virus-infected cells and no decrease in lipid peroxides was observed in daidzein-treated cells. In daidzein-treated cells, 5-hydroxyeicosatetraenoic acid, the 5-lipoxygenase product derived from arachidonate, was significantly elevated compared to other lipid peroxides. Zileuton (5-lipoxygenase inhibitor) and 5-lipoxygenase knockdown reduced the daidzein-induced antiviral effect. Moreover, virus replication was regulated by treatment with 5-hydroperoxyeicosatetraenoic acid, a precursor of 5-hydroxyeicosatetraenoic acid and 5-lipoxygenase primary product. These results suggest that daidzein regulates virus replication via signal transduction through 5-lipoxygenase products.</p></abstract><kwd-group kwd-group-type="author"><kwd>influenza virus</kwd><kwd>daidzein</kwd><kwd>lipid peroxide</kwd><kwd>signal transduction</kwd><kwd>lipoxygenase</kwd></kwd-group></article-meta></front><floats-group><fig id="F1" orientation="portrait" position="float"><label>Fig. 1</label><caption><p>Effect of daidzein on multiplication of influenza virus and on proliferation of MDCK cells. MDCK cells were inoculated with influenza A/PR/8/34 virus at a MOI of 0.001. (A) Concentration-dependent inhibitory effect of Daidzein on virus multiplication. Viral titers were determined at 24 h post-infection by focus-forming assays. (B) Cytotoxicity of daidzein. The cell viability of MDCK cells was determined at 24 h post-addition of daidzein by WST-8 assay. The dotted lines indicate a daidzein concentration of 275 µM. Data are presented as mean ± SD (<italic>n</italic> = 3). Data are representative of three independent experiments. <bold>*</bold><italic>p</italic><0.01; <bold>**</bold><italic>p</italic><0.05; <bold>***</bold><italic>p</italic><0.0025; <bold>****</bold><italic>p</italic><0.001.</p></caption><graphic xlink:href="jcbn19-70f01"></graphic></fig><fig id="F2" orientation="portrait" position="float"><label>Fig. 2</label><caption><p>Effect of daidzein on production of lipid peroxide. MDCK cells were inoculated with influenza A/PR/8/34 virus at a MOI of 0.001. Lipid fraction was harvested from MDCK cells at 24 h post-infection. (A) 7β-OHCh, (B) tHODE, (C) 8-iso-PGF<sub>2α</sub>, (D) 12-HETE, (E) 15-HETE, and (F) 5-HETE in cells. Data are presented as mean ± SD (<italic>n</italic> = 3). Data are representative of three independent experiments. <bold>*</bold><italic>p</italic><0.01, <bold>**</bold><italic>p</italic><0.005.</p></caption><graphic xlink:href="jcbn19-70f02"></graphic></fig><fig id="F3" orientation="portrait" position="float"><label>Fig. 3</label><caption><p>Effect of daidzein on lipoxygenase activity in MDCK cell lysate. The cell lysates were obtained from MDCK cells treated for 24 h with or without daidzein. AA was added to cell lysates to a final concentration at 120 µM and incubated for 180 min at 37°C with gentle mixing. After 180 min incubation, lipids were extracted and 5-, 12-, and 15-HETE and AA were measured by LC-MS/MS. The 5-, 12-, and 15-LOX activities in MDCK cell lysates were evaluated as the ratio of each HETE to AA. (A) 5-LOX activity was evaluated as 5-HETE/AA, (B) 12-LOX activity was evaluated as 12-HETE/AA, and (C) 15-LOX activity was evaluated as 15-HETE/AA. Data are presented as mean ± SD (<italic>n</italic> = 3). Data are representative of three independent experiments. <bold>*</bold><italic>p</italic><0.05.</p></caption><graphic xlink:href="jcbn19-70f03"></graphic></fig><fig id="F4" orientation="portrait" position="float"><label>Fig. 4</label><caption><p>Effect of 5-LOX inhibitor, zileuton, and 5-LOX siRNA transfection on multiplication of influenza virus and production of 5-HETE. MDCK cells were inoculated with influenza A/PR/8/34 virus at a MOI of 0.001. Viral titers were determined at 24 h post-infection by focus-forming assays. (A) Effect of zileuton on the titer of influenza virus. (B) Effect of zileuton on 5-HETE production. (C) Effect of 5-LOX siRNA on 5-LOX expression in whole cell lysate of MDCK cells treated with or without daidzein and with or without influenza virus infection. (D) Effect of 5-LOX siRNA on daidzein-induced inhibition of virus multiplication. Data are presented as mean ± SD. Data are representative of three independent experiments. <bold>*</bold><italic>p</italic><0.05, <bold>**</bold><italic>p</italic><0.025, <bold>***</bold><italic>p</italic><0.005, <bold>****</bold><italic>p</italic><0.001.</p></caption><graphic xlink:href="jcbn19-70f04"></graphic></fig><fig id="F5" orientation="portrait" position="float"><label>Fig. 5</label><caption><p>Effect of 5-HpETE on multiplication of influenza virus. MDCK cells were inoculated with influenza A/PR/8/34 virus at a MOI of 0.001. (A) Concentration-dependent inhibitory effect of 5-HpETE on virus multiplication. The inhibition rate of the virus titer was determined at 24 h post-infection by focus-forming assays. (B) Cytotoxicity of 5-HpETE. The survival rate of MDCK cells was determined at 24 h post-infection by WST-8 assay. Data are presented as mean ± SD (<italic>n</italic> = 3). Data are representative of three independent experiments.</p></caption><graphic xlink:href="jcbn19-70f05"></graphic></fig></floats-group></pmc><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Horio, Yuka" sort="Horio, Yuka" uniqKey="Horio Y" first="Yuka" last="Horio">Yuka Horio</name></noRegion><name sortKey="Isegawa, Yuji" sort="Isegawa, Yuji" uniqKey="Isegawa Y" first="Yuji" last="Isegawa">Yuji Isegawa</name><name sortKey="Isegawa, Yuji" sort="Isegawa, Yuji" uniqKey="Isegawa Y" first="Yuji" last="Isegawa">Yuji Isegawa</name><name sortKey="Ishida, Noriko" sort="Ishida, Noriko" uniqKey="Ishida N" first="Noriko" last="Ishida">Noriko Ishida</name><name sortKey="Morimoto, Ryosuke" sort="Morimoto, Ryosuke" uniqKey="Morimoto R" first="Ryosuke" last="Morimoto">Ryosuke Morimoto</name><name sortKey="Ohshima, Atsushi" sort="Ohshima, Atsushi" uniqKey="Ohshima A" first="Atsushi" last="Ohshima">Atsushi Ohshima</name><name sortKey="Shichiri, Mototada" sort="Shichiri, Mototada" uniqKey="Shichiri M" first="Mototada" last="Shichiri">Mototada Shichiri</name><name sortKey="Shichiri, Mototada" sort="Shichiri, Mototada" uniqKey="Shichiri M" first="Mototada" last="Shichiri">Mototada Shichiri</name><name sortKey="Sogabe, Riho" sort="Sogabe, Riho" uniqKey="Sogabe R" first="Riho" last="Sogabe">Riho Sogabe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E78 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000E78 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= PMC:6983437
|texte= Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:32001954" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |