Cisplatin bioconjugated enzymatic GNPs amplify the effect of cisplatin with acquiescence
Identifieur interne : 000A53 ( Ncbi/Merge ); précédent : 000A52; suivant : 000A54Cisplatin bioconjugated enzymatic GNPs amplify the effect of cisplatin with acquiescence
Auteurs : Sana Iram [Inde] ; Manaal Zahera [Inde] ; Iram Wahid [Inde] ; Abu Baker [Inde] ; Mohammad Raish [Arabie saoudite] ; Altaf Khan [Arabie saoudite] ; Naushad Ali [Arabie saoudite] ; Saheem Ahmad [Inde] ; Mohd Sajid Khan [Inde]Source :
- Scientific Reports [ 2045-2322 ] ; 2019.
Abstract
Enzymatic gold nanoparticles (B-GNPs) have been synthesized using a natural anticancer agent bromelain (a cysteine protease) and these nanoparticles were used to bioconjugate Cisplatin (highly effective against osteosarcoma and lung cancer). Cisplatin bioconjugated bromelain encapsulated gold nanoparticles (B-C-GNPs) were found profoundly potent against same cancers at much lower concentration with minimum side effects due to the synergistic effect of bromelain. The B-C-GNPs have been observed to inhibit the proliferation of osteosarcoma cell lines Saos-2 and MG-63 with IC50 estimation of 4.51 µg/ml and 3.21 µg/ml, respectively, and against small lung cancer cell line A-549 with IC50 2.5 µg/ml which is lower than IC50 of cisplatin against same cell lines. The B-GNPs/B-C-GNPs were characterized by TEM, UV-Visible spectroscopy, Zeta potential and DLS to confirm the production, purity, crystalline nature, stability of nanoemulsion, size and shape distribution. The change in 2D and 3D conformation of bromelain after encapsulation was studied by Circular Dichroism and Fluorometry, respectively. It was found that after encapsulation, a 19.4% loss in secondary structure was observed, but tertiary structure was not altered significantly and this loss improved the anticancer activity. The confirmation of bioconjugation of cisplatin with B-GNPs was done by UV-Visible spectroscopy, TEM, FTIR, 2D 1H NMR DOSY and ICP-MS. Further, it was found that almost ~4 cisplatin molecules bound with each B-GNPs nanoparticle.
Url:
DOI: 10.1038/s41598-019-50215-y
PubMed: 31554850
PubMed Central: 6761153
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<front><div type="abstract" xml:lang="en"><p id="Par1">Enzymatic gold nanoparticles (B-GNPs) have been synthesized using a natural anticancer agent bromelain (a cysteine protease) and these nanoparticles were used to bioconjugate Cisplatin (highly effective against osteosarcoma and lung cancer). Cisplatin bioconjugated bromelain encapsulated gold nanoparticles (B-C-GNPs) were found profoundly potent against same cancers at much lower concentration with minimum side effects due to the synergistic effect of bromelain. The B-C-GNPs have been observed to inhibit the proliferation of osteosarcoma cell lines Saos-2 and MG-63 with IC<sub>50</sub>
estimation of 4.51 µg/ml and 3.21 µg/ml, respectively, and against small lung cancer cell line A-549 with IC<sub>50</sub>
2.5 µg/ml which is lower than IC<sub>50</sub>
of cisplatin against same cell lines. The B-GNPs/B-C-GNPs were characterized by TEM, UV-Visible spectroscopy, Zeta potential and DLS to confirm the production, purity, crystalline nature, stability of nanoemulsion, size and shape distribution. The change in 2D and 3D conformation of bromelain after encapsulation was studied by Circular Dichroism and Fluorometry, respectively. It was found that after encapsulation, a 19.4% loss in secondary structure was observed, but tertiary structure was not altered significantly and this loss improved the anticancer activity. The confirmation of bioconjugation of cisplatin with B-GNPs was done by UV-Visible spectroscopy, TEM, FTIR, 2D <sup>1</sup>
H NMR DOSY and ICP-MS. Further, it was found that almost ~4 cisplatin molecules bound with each B-GNPs nanoparticle.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id>
<journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id>
<journal-title-group><journal-title>Scientific Reports</journal-title>
</journal-title-group>
<issn pub-type="epub">2045-2322</issn>
<publisher><publisher-name>Nature Publishing Group UK</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">31554850</article-id>
<article-id pub-id-type="pmc">6761153</article-id>
<article-id pub-id-type="publisher-id">50215</article-id>
<article-id pub-id-type="doi">10.1038/s41598-019-50215-y</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Cisplatin bioconjugated enzymatic GNPs amplify the effect of cisplatin with acquiescence</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Iram</surname>
<given-names>Sana</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zahera</surname>
<given-names>Manaal</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wahid</surname>
<given-names>Iram</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Baker</surname>
<given-names>Abu</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Raish</surname>
<given-names>Mohammad</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Khan</surname>
<given-names>Altaf</given-names>
</name>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ali</surname>
<given-names>Naushad</given-names>
</name>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ahmad</surname>
<given-names>Saheem</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Khan</surname>
<given-names>Mohd Sajid</given-names>
</name>
<address><email>research.sajid@gmail.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1756 4240</institution-id>
<institution-id institution-id-type="GRID">grid.411723.2</institution-id>
<institution>Nanomedicine & Nanobiotechnology Lab, Department of Biosciences,</institution>
<institution>Integral University,</institution>
</institution-wrap>
Lucknow, India</aff>
<aff id="Aff2"><label>2</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1773 5396</institution-id>
<institution-id institution-id-type="GRID">grid.56302.32</institution-id>
<institution>Department Pharmaceutics, College of Pharmacy,</institution>
<institution>King Saud University,</institution>
</institution-wrap>
Riyadh, 11451 Saudi Arabia</aff>
<aff id="Aff3"><label>3</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1773 5396</institution-id>
<institution-id institution-id-type="GRID">grid.56302.32</institution-id>
<institution>Department Pharmacology and Toxicology,</institution>
<institution>College of Pharmacy, King Saud University,</institution>
</institution-wrap>
Riyadh, 11451 Saudi Arabia</aff>
<aff id="Aff4"><label>4</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1773 5396</institution-id>
<institution-id institution-id-type="GRID">grid.56302.32</institution-id>
<institution>Quality Assurance Unit, College of Pharmacy,</institution>
<institution>King Saud University,</institution>
</institution-wrap>
Riyadh, 11451 Saudi Arabia</aff>
</contrib-group>
<pub-date pub-type="epub"><day>25</day>
<month>9</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>25</day>
<month>9</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="collection"><year>2019</year>
</pub-date>
<volume>9</volume>
<elocation-id>13826</elocation-id>
<history><date date-type="received"><day>11</day>
<month>1</month>
<year>2018</year>
</date>
<date date-type="accepted"><day>29</day>
<month>3</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s) 2019</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p id="Par1">Enzymatic gold nanoparticles (B-GNPs) have been synthesized using a natural anticancer agent bromelain (a cysteine protease) and these nanoparticles were used to bioconjugate Cisplatin (highly effective against osteosarcoma and lung cancer). Cisplatin bioconjugated bromelain encapsulated gold nanoparticles (B-C-GNPs) were found profoundly potent against same cancers at much lower concentration with minimum side effects due to the synergistic effect of bromelain. The B-C-GNPs have been observed to inhibit the proliferation of osteosarcoma cell lines Saos-2 and MG-63 with IC<sub>50</sub>
estimation of 4.51 µg/ml and 3.21 µg/ml, respectively, and against small lung cancer cell line A-549 with IC<sub>50</sub>
2.5 µg/ml which is lower than IC<sub>50</sub>
of cisplatin against same cell lines. The B-GNPs/B-C-GNPs were characterized by TEM, UV-Visible spectroscopy, Zeta potential and DLS to confirm the production, purity, crystalline nature, stability of nanoemulsion, size and shape distribution. The change in 2D and 3D conformation of bromelain after encapsulation was studied by Circular Dichroism and Fluorometry, respectively. It was found that after encapsulation, a 19.4% loss in secondary structure was observed, but tertiary structure was not altered significantly and this loss improved the anticancer activity. The confirmation of bioconjugation of cisplatin with B-GNPs was done by UV-Visible spectroscopy, TEM, FTIR, 2D <sup>1</sup>
H NMR DOSY and ICP-MS. Further, it was found that almost ~4 cisplatin molecules bound with each B-GNPs nanoparticle.</p>
</abstract>
<kwd-group kwd-group-type="npg-subject"><title>Subject terms</title>
<kwd>Nanoparticles</kwd>
<kwd>Lung cancer</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution>The authors SI and MZ thank University Grant commission (UGC), New Delhi, for Maulana Azad National Fellowship and Uttar Pradesh Council of Science and Technology (UPCST), Lucknow, respectively. The authors also acknowledge the Integral University and Department of Biosciences for providing instrumentation facilities and support for the research.</institution>
</funding-source>
</award-group>
</funding-group>
<funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">https://doi.org/10.13039/501100002383</institution-id>
<institution>King Saud University (KSU)</institution>
</institution-wrap>
</funding-source>
<award-id>RG-1438-081</award-id>
<award-id>RG-1438-081</award-id>
<award-id>RG-1438-081</award-id>
<principal-award-recipient><name><surname>Raish</surname>
<given-names>Mohammad</given-names>
</name>
<name><surname>Khan</surname>
<given-names>Altaf</given-names>
</name>
<name><surname>Ali</surname>
<given-names>Naushad</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2019</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Arabie saoudite</li>
<li>Inde</li>
</country>
</list>
<tree><country name="Inde"><noRegion><name sortKey="Iram, Sana" sort="Iram, Sana" uniqKey="Iram S" first="Sana" last="Iram">Sana Iram</name>
</noRegion>
<name sortKey="Ahmad, Saheem" sort="Ahmad, Saheem" uniqKey="Ahmad S" first="Saheem" last="Ahmad">Saheem Ahmad</name>
<name sortKey="Baker, Abu" sort="Baker, Abu" uniqKey="Baker A" first="Abu" last="Baker">Abu Baker</name>
<name sortKey="Khan, Mohd Sajid" sort="Khan, Mohd Sajid" uniqKey="Khan M" first="Mohd Sajid" last="Khan">Mohd Sajid Khan</name>
<name sortKey="Wahid, Iram" sort="Wahid, Iram" uniqKey="Wahid I" first="Iram" last="Wahid">Iram Wahid</name>
<name sortKey="Zahera, Manaal" sort="Zahera, Manaal" uniqKey="Zahera M" first="Manaal" last="Zahera">Manaal Zahera</name>
</country>
<country name="Arabie saoudite"><noRegion><name sortKey="Raish, Mohammad" sort="Raish, Mohammad" uniqKey="Raish M" first="Mohammad" last="Raish">Mohammad Raish</name>
</noRegion>
<name sortKey="Ali, Naushad" sort="Ali, Naushad" uniqKey="Ali N" first="Naushad" last="Ali">Naushad Ali</name>
<name sortKey="Khan, Altaf" sort="Khan, Altaf" uniqKey="Khan A" first="Altaf" last="Khan">Altaf Khan</name>
</country>
</tree>
</affiliations>
</record>
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