The identification and characterization of an uptake system for taurine into rat lung slices.
Identifieur interne : 000092 ( Ncbi/Merge ); précédent : 000091; suivant : 000093The identification and characterization of an uptake system for taurine into rat lung slices.
Auteurs : C P Lewis [Royaume-Uni] ; G M Cohen ; L L SmithSource :
- Biochemical pharmacology [ 0006-2952 ] ; 1990.
Descripteurs français
- KwdFr :
- Adénosine triphosphate (pharmacologie), Animaux, Antimycine A (pharmacologie), Chloroquine (pharmacologie), Chlorpromazine (pharmacologie), Cinétique, Colforsine (pharmacologie), Cyanure de potassium (pharmacologie), Dibutyryl AMP cyclique (pharmacologie), Fixation compétitive, Lignées consanguines de rats, Mâle, Poumon (), Poumon (métabolisme), Rats, Sodium (pharmacologie), Taurine (métabolisme), Tétraméthyl-diazènedicarboxamide (pharmacologie), Xanthine(isobutyl-3 methyl-1) (pharmacologie).
- MESH :
- métabolisme : Poumon, Taurine.
- pharmacologie : Adénosine triphosphate, Antimycine A, Chloroquine, Chlorpromazine, Colforsine, Cyanure de potassium, Dibutyryl AMP cyclique, Sodium, Tétraméthyl-diazènedicarboxamide, Xanthine(isobutyl-3 methyl-1).
- Animaux, Cinétique, Fixation compétitive, Lignées consanguines de rats, Mâle, Poumon, Rats.
English descriptors
- KwdEn :
- 1-Methyl-3-isobutylxanthine (pharmacology), Adenosine Triphosphate (pharmacology), Animals, Antimycin A (pharmacology), Binding, Competitive, Bucladesine (pharmacology), Chloroquine (pharmacology), Chlorpromazine (pharmacology), Colforsin (pharmacology), Diamide (pharmacology), Kinetics, Lung (drug effects), Lung (metabolism), Male, Potassium Cyanide (pharmacology), Rats, Rats, Inbred Strains, Sodium (pharmacology), Taurine (metabolism).
- MESH :
- chemical , metabolism : Taurine.
- chemical , pharmacology : 1-Methyl-3-isobutylxanthine, Adenosine Triphosphate, Antimycin A, Bucladesine, Chloroquine, Chlorpromazine, Colforsin, Diamide, Potassium Cyanide, Sodium.
- drug effects : Lung.
- metabolism : Lung.
- Animals, Binding, Competitive, Kinetics, Male, Rats, Rats, Inbred Strains.
Abstract
The objective of this study was to determine whether taurine was accumulated by rat lung slices and if so, to establish the role of this uptake as a source of pulmonary taurine. We have shown that taurine is accumulated into rat lung by an active uptake process that was both ATP and Na(+)-dependent and obeyed saturation kinetics, exhibiting an apparent Km of 186 microM and Vmax of 970 nmol/g wet wt/hr. Substrate specificity of the system was high and only compounds possessing anionic and cationic groups separated by two methylene groups were able to competitively inhibit taurine uptake. Subsequent to its uptake, taurine was not significantly metabolized, and since the apparent Km for the uptake process is similar to the known plasma concentration of taurine, it can be inferred that this system will contribute to pulmonary taurine uptake in vivo. Taurine has been suggested to possess antioxidant and antiinflammatory properties, and we suggest that this uptake system may contribute to the defence of pulmonary tissue against oxidative stress.
DOI: 10.1016/0006-2952(90)90047-o
PubMed: 1689575
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Imperial Chemical Industries PLC, Macclesfield, Cheshire, U.K.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Biochemical Toxicology Section, Imperial Chemical Industries PLC, Macclesfield, Cheshire</wicri:regionArea><wicri:noRegion>Cheshire</wicri:noRegion></affiliation></author><author><name sortKey="Cohen, G M" sort="Cohen, G M" uniqKey="Cohen G" first="G M" last="Cohen">G M Cohen</name></author><author><name sortKey="Smith, L L" sort="Smith, L L" uniqKey="Smith L" first="L L" last="Smith">L L Smith</name></author></analytic><series><title level="j">Biochemical pharmacology</title><idno type="ISSN">0006-2952</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>1-Methyl-3-isobutylxanthine (pharmacology)</term><term>Adenosine Triphosphate (pharmacology)</term><term>Animals</term><term>Antimycin A (pharmacology)</term><term>Binding, Competitive</term><term>Bucladesine (pharmacology)</term><term>Chloroquine (pharmacology)</term><term>Chlorpromazine (pharmacology)</term><term>Colforsin (pharmacology)</term><term>Diamide (pharmacology)</term><term>Kinetics</term><term>Lung (drug effects)</term><term>Lung (metabolism)</term><term>Male</term><term>Potassium Cyanide (pharmacology)</term><term>Rats</term><term>Rats, Inbred Strains</term><term>Sodium (pharmacology)</term><term>Taurine (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adénosine triphosphate (pharmacologie)</term><term>Animaux</term><term>Antimycine A (pharmacologie)</term><term>Chloroquine (pharmacologie)</term><term>Chlorpromazine (pharmacologie)</term><term>Cinétique</term><term>Colforsine (pharmacologie)</term><term>Cyanure de potassium (pharmacologie)</term><term>Dibutyryl AMP cyclique (pharmacologie)</term><term>Fixation compétitive</term><term>Lignées consanguines de rats</term><term>Mâle</term><term>Poumon ()</term><term>Poumon (métabolisme)</term><term>Rats</term><term>Sodium (pharmacologie)</term><term>Taurine (métabolisme)</term><term>Tétraméthyl-diazènedicarboxamide (pharmacologie)</term><term>Xanthine(isobutyl-3 methyl-1) (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Taurine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>1-Methyl-3-isobutylxanthine</term><term>Adenosine Triphosphate</term><term>Antimycin A</term><term>Bucladesine</term><term>Chloroquine</term><term>Chlorpromazine</term><term>Colforsin</term><term>Diamide</term><term>Potassium Cyanide</term><term>Sodium</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Poumon</term><term>Taurine</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Adénosine triphosphate</term><term>Antimycine A</term><term>Chloroquine</term><term>Chlorpromazine</term><term>Colforsine</term><term>Cyanure de potassium</term><term>Dibutyryl AMP cyclique</term><term>Sodium</term><term>Tétraméthyl-diazènedicarboxamide</term><term>Xanthine(isobutyl-3 methyl-1)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Binding, Competitive</term><term>Kinetics</term><term>Male</term><term>Rats</term><term>Rats, Inbred Strains</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cinétique</term><term>Fixation compétitive</term><term>Lignées consanguines de rats</term><term>Mâle</term><term>Poumon</term><term>Rats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective of this study was to determine whether taurine was accumulated by rat lung slices and if so, to establish the role of this uptake as a source of pulmonary taurine. We have shown that taurine is accumulated into rat lung by an active uptake process that was both ATP and Na(+)-dependent and obeyed saturation kinetics, exhibiting an apparent Km of 186 microM and Vmax of 970 nmol/g wet wt/hr. Substrate specificity of the system was high and only compounds possessing anionic and cationic groups separated by two methylene groups were able to competitively inhibit taurine uptake. Subsequent to its uptake, taurine was not significantly metabolized, and since the apparent Km for the uptake process is similar to the known plasma concentration of taurine, it can be inferred that this system will contribute to pulmonary taurine uptake in vivo. Taurine has been suggested to possess antioxidant and antiinflammatory properties, and we suggest that this uptake system may contribute to the defence of pulmonary tissue against oxidative stress.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1689575</PMID><DateCompleted><Year>1990</Year><Month>03</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-2952</ISSN><JournalIssue CitedMedium="Print"><Volume>39</Volume><Issue>3</Issue><PubDate><Year>1990</Year><Month>Feb</Month><Day>01</Day></PubDate></JournalIssue><Title>Biochemical pharmacology</Title><ISOAbbreviation>Biochem. Pharmacol.</ISOAbbreviation></Journal><ArticleTitle>The identification and characterization of an uptake system for taurine into rat lung slices.</ArticleTitle><Pagination><MedlinePgn>431-7</MedlinePgn></Pagination><Abstract><AbstractText>The objective of this study was to determine whether taurine was accumulated by rat lung slices and if so, to establish the role of this uptake as a source of pulmonary taurine. We have shown that taurine is accumulated into rat lung by an active uptake process that was both ATP and Na(+)-dependent and obeyed saturation kinetics, exhibiting an apparent Km of 186 microM and Vmax of 970 nmol/g wet wt/hr. Substrate specificity of the system was high and only compounds possessing anionic and cationic groups separated by two methylene groups were able to competitively inhibit taurine uptake. Subsequent to its uptake, taurine was not significantly metabolized, and since the apparent Km for the uptake process is similar to the known plasma concentration of taurine, it can be inferred that this system will contribute to pulmonary taurine uptake in vivo. Taurine has been suggested to possess antioxidant and antiinflammatory properties, and we suggest that this uptake system may contribute to the defence of pulmonary tissue against oxidative stress.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lewis</LastName><ForeName>C P</ForeName><Initials>CP</Initials><AffiliationInfo><Affiliation>Biochemical Toxicology Section, Imperial Chemical Industries PLC, Macclesfield, Cheshire, U.K.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cohen</LastName><ForeName>G M</ForeName><Initials>GM</Initials></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>L L</ForeName><Initials>LL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biochem Pharmacol</MedlineTA><NlmUniqueID>0101032</NlmUniqueID><ISSNLinking>0006-2952</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>10465-78-8</RegistryNumber><NameOfSubstance UI="D003958">Diamide</NameOfSubstance></Chemical><Chemical><RegistryNumber>1EQV5MLY3D</RegistryNumber><NameOfSubstance UI="D013654">Taurine</NameOfSubstance></Chemical><Chemical><RegistryNumber>1F7A44V6OU</RegistryNumber><NameOfSubstance UI="D005576">Colforsin</NameOfSubstance></Chemical><Chemical><RegistryNumber>63X7MBT2LQ</RegistryNumber><NameOfSubstance UI="D003994">Bucladesine</NameOfSubstance></Chemical><Chemical><RegistryNumber>642-15-9</RegistryNumber><NameOfSubstance UI="D000968">Antimycin A</NameOfSubstance></Chemical><Chemical><RegistryNumber>886U3H6UFF</RegistryNumber><NameOfSubstance UI="D002738">Chloroquine</NameOfSubstance></Chemical><Chemical><RegistryNumber>8L70Q75FXE</RegistryNumber><NameOfSubstance UI="D000255">Adenosine Triphosphate</NameOfSubstance></Chemical><Chemical><RegistryNumber>9NEZ333N27</RegistryNumber><NameOfSubstance UI="D012964">Sodium</NameOfSubstance></Chemical><Chemical><RegistryNumber>MQD255M2ZO</RegistryNumber><NameOfSubstance UI="D011190">Potassium Cyanide</NameOfSubstance></Chemical><Chemical><RegistryNumber>TBT296U68M</RegistryNumber><NameOfSubstance UI="D015056">1-Methyl-3-isobutylxanthine</NameOfSubstance></Chemical><Chemical><RegistryNumber>U42B7VYA4P</RegistryNumber><NameOfSubstance UI="D002746">Chlorpromazine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015056" MajorTopicYN="N">1-Methyl-3-isobutylxanthine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000255" MajorTopicYN="N">Adenosine Triphosphate</DescriptorName><QualifierName UI="Q000494" 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UI="D005576" MajorTopicYN="N">Colforsin</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003958" MajorTopicYN="N">Diamide</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011190" MajorTopicYN="N">Potassium Cyanide</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011919" MajorTopicYN="N">Rats, Inbred Strains</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012964" MajorTopicYN="N">Sodium</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013654" MajorTopicYN="N">Taurine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>2</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1990</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1689575</ArticleId><ArticleId IdType="pii">0006-2952(90)90047-O</ArticleId><ArticleId IdType="doi">10.1016/0006-2952(90)90047-o</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Cohen, G M" sort="Cohen, G M" uniqKey="Cohen G" first="G M" last="Cohen">G M Cohen</name><name sortKey="Smith, L L" sort="Smith, L L" uniqKey="Smith L" first="L L" last="Smith">L L Smith</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Lewis, C P" sort="Lewis, C P" uniqKey="Lewis C" first="C P" last="Lewis">C P Lewis</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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