Intracellular transport and esterification of exchangeable cholesterol in cultured human lung fibroblasts.
Identifieur interne : 001131 ( Ncbi/Curation ); précédent : 001130; suivant : 001132Intracellular transport and esterification of exchangeable cholesterol in cultured human lung fibroblasts.
Auteurs : J P Slotte ; B. Lundberg ; S. BjörkerudSource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 1984.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Cholestérol, Cholestérol ester, Phospholipides, Poumon.
- pharmacologie : Colchicine, Cyanures, Cytochalasine B, Fluorures.
- Cellules cultivées, Humains, Pinocytose.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cholesterol, Cholesterol Esters, Phospholipids.
- chemical , pharmacology : Colchicine, Cyanides, Cytochalasin B, Fluorides.
- drug effects : Pinocytosis.
- metabolism : Lung.
- Cells, Cultured, Humans.
Abstract
We have studied the intracellular fate of exchangeable cholesterol in a model system with lipid vesicles (cholesterol/phospholipid mole ratio 1:1) and cultured human lung fibroblasts. Exchangeable [3H]cholesterol in lipid vesicles was readily incorporated into cellular plasma membranes and transported to intracellular esterification sites. The formation of [3H]cholesteryl esters was not affected by cytoskeleton-disrupting drugs. A reduction of cellular pinocytosis by 75% did not reduce the formation of tracer-labelled esters, suggesting that membrane flow due to the energy-dependent pinocytosis is no major contributor to the intracellular transport of molecular cholesterol between plasma membranes and esterification sites. The formation of [3H]cholesteryl esters was not significantly affected by energy poisons (NaF and KCN) but was inhibited (to 50%) by chloroquine at 50 microM. This may indicate that membrane-derived cholesterol passes through the lysosomal compartment on its way to intracellular esterification sites.
DOI: 10.1016/0005-2760(84)90258-3
PubMed: 6712979
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pubmed:6712979Le document en format XML
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Lundberg</name></author><author><name sortKey="Bjorkerud, S" sort="Bjorkerud, S" uniqKey="Bjorkerud S" first="S" last="Björkerud">S. Björkerud</name></author></analytic><series><title level="j">Biochimica et biophysica acta</title><idno type="ISSN">0006-3002</idno><imprint><date when="1984" type="published">1984</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cells, Cultured</term><term>Cholesterol (metabolism)</term><term>Cholesterol Esters (metabolism)</term><term>Colchicine (pharmacology)</term><term>Cyanides (pharmacology)</term><term>Cytochalasin B (pharmacology)</term><term>Fluorides (pharmacology)</term><term>Humans</term><term>Lung (metabolism)</term><term>Phospholipids (metabolism)</term><term>Pinocytosis (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules cultivées</term><term>Cholestérol (métabolisme)</term><term>Cholestérol ester (métabolisme)</term><term>Colchicine (pharmacologie)</term><term>Cyanures (pharmacologie)</term><term>Cytochalasine B (pharmacologie)</term><term>Fluorures (pharmacologie)</term><term>Humains</term><term>Phospholipides (métabolisme)</term><term>Pinocytose ()</term><term>Poumon (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cholesterol</term><term>Cholesterol Esters</term><term>Phospholipids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Colchicine</term><term>Cyanides</term><term>Cytochalasin B</term><term>Fluorides</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Pinocytosis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cholestérol</term><term>Cholestérol ester</term><term>Phospholipides</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Colchicine</term><term>Cyanures</term><term>Cytochalasine B</term><term>Fluorures</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Humains</term><term>Pinocytose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have studied the intracellular fate of exchangeable cholesterol in a model system with lipid vesicles (cholesterol/phospholipid mole ratio 1:1) and cultured human lung fibroblasts. Exchangeable [3H]cholesterol in lipid vesicles was readily incorporated into cellular plasma membranes and transported to intracellular esterification sites. The formation of [3H]cholesteryl esters was not affected by cytoskeleton-disrupting drugs. A reduction of cellular pinocytosis by 75% did not reduce the formation of tracer-labelled esters, suggesting that membrane flow due to the energy-dependent pinocytosis is no major contributor to the intracellular transport of molecular cholesterol between plasma membranes and esterification sites. The formation of [3H]cholesteryl esters was not significantly affected by energy poisons (NaF and KCN) but was inhibited (to 50%) by chloroquine at 50 microM. This may indicate that membrane-derived cholesterol passes through the lysosomal compartment on its way to intracellular esterification sites.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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