Functional cloning using pFB retroviral cDNA expression libraries
Identifieur interne : 002248 ( Main/Merge ); précédent : 002247; suivant : 002249Functional cloning using pFB retroviral cDNA expression libraries
Auteurs : Katherine A. Felts [États-Unis] ; Keith Chen [États-Unis] ; Kim Zaharee [États-Unis] ; Latha Sundar [États-Unis] ; Jamie Limjoco [États-Unis] ; Anna Miller [États-Unis] ; Peter Vaillancourt [États-Unis]Source :
- Molecular Biotechnology [ 1073-6085 ] ; 2002-09-01.
English descriptors
Abstract
Abstract: Retroviral cDNA expression libraries allow the efficient introduction of complex cDNA libraries into virtually any mitotic cell type for screening based on gene function. The cDNA copy number per cell can be easily controlled by adjusting the multiplicity of infection, thus cell populations may be generated in which >90% of infected cells contain one to three cDNAs. We describe the isolation of two known oncogenes and one cell-surface receptor from a human Burkitt’s lymphoma (Daudi) cDNA library inserted into the hightiter retroviral vector pFB.
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DOI: 10.1385/MB:22:1:025
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