DNA Repair Interacts with Autophagy To Regulate Inflammatory Responses to Pulmonary Hyperoxia.
Identifieur interne : 000D21 ( Main/Merge ); précédent : 000D20; suivant : 000D22DNA Repair Interacts with Autophagy To Regulate Inflammatory Responses to Pulmonary Hyperoxia.
Auteurs : Yan Ye [États-Unis] ; Ping Lin [États-Unis] ; Weidong Zhang [États-Unis] ; Shirui Tan [États-Unis] ; Xikun Zhou [États-Unis] ; Rongpeng Li [États-Unis] ; Qinqin Pu [États-Unis] ; Jonathan L. Koff [États-Unis] ; Archana Dhasarathy [États-Unis] ; Feng Ma [Japon] ; Xin Deng [Japon] ; Jianxin Jiang [Japon] ; Min Wu [Japon]Source :
- Journal of immunology (Baltimore, Md. : 1950) [ 1550-6606 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux, Autophagie, Cytokines (biosynthèse), DNA Glycosylases (métabolisme), Hyperoxie (anatomopathologie), Hyperoxie (métabolisme), Immunoprécipitation, Immunoprécipitation de la chromatine, Inflammation (anatomopathologie), Inflammation (métabolisme), Lésion pulmonaire aigüe (anatomopathologie), Lésion pulmonaire aigüe (métabolisme), Modèles animaux de maladie humaine, Poumon (anatomopathologie), Poumon (métabolisme), Réparation de l'ADN, Souris, Souris de lignée C57BL, Souris knockout, Technique de Western, Test des comètes.
- MESH :
- anatomopathologie : Hyperoxie, Inflammation, Lésion pulmonaire aigüe, Poumon.
- biosynthèse : Cytokines.
- métabolisme : DNA Glycosylases, Hyperoxie, Inflammation, Lésion pulmonaire aigüe, Poumon.
- Animaux, Autophagie, Immunoprécipitation, Immunoprécipitation de la chromatine, Modèles animaux de maladie humaine, Réparation de l'ADN, Souris, Souris de lignée C57BL, Souris knockout, Technique de Western, Test des comètes.
English descriptors
- KwdEn :
- Acute Lung Injury (metabolism), Acute Lung Injury (pathology), Animals, Autophagy, Blotting, Western, Chromatin Immunoprecipitation, Comet Assay, Cytokines (biosynthesis), DNA Glycosylases (metabolism), DNA Repair, Disease Models, Animal, Hyperoxia (metabolism), Hyperoxia (pathology), Immunoprecipitation, Inflammation (metabolism), Inflammation (pathology), Lung (metabolism), Lung (pathology), Mice, Mice, Inbred C57BL, Mice, Knockout.
- MESH :
- chemical , biosynthesis : Cytokines.
- metabolism : Acute Lung Injury, DNA Glycosylases, Hyperoxia, Inflammation, Lung.
- pathology : Acute Lung Injury, Hyperoxia, Inflammation, Lung.
- Animals, Autophagy, Blotting, Western, Chromatin Immunoprecipitation, Comet Assay, DNA Repair, Disease Models, Animal, Immunoprecipitation, Mice, Mice, Inbred C57BL, Mice, Knockout.
Abstract
Oxygen is supplied as a supportive treatment for patients suffering from acute respiratory distress syndrome. Unfortunately, high oxygen concentration increases reactive oxygen species generation, which causes DNA damage and ultimately cell death in the lung. Although 8-oxoguanine-DNA glycosylase (OGG-1) is involved in repairing hyperoxia-mediated DNA damage, the underlying molecular mechanism remains elusive. In this study, we report that ogg-1-deficient mice exhibited a significant increase of proinflammatory cytokines (TNF-α, IL-6, and IFN-γ) in the lung after being exposed to 95% oxygen. In addition, we found that ogg-1 deficiency downregulated (macro)autophagy when exposed to hyperoxia both in vitro and in vivo, which was evident by decreased conversion of LC3-I to LC3-II, reduced LC3 punctate staining, and lower Atg7 expression compared with controls. Using a chromatin immunoprecipitation assay, we found that OGG-1 associated with the promoter of Atg7, suggesting a role for OGG1 in regulation of Atg7 activity. Knocking down OGG-1 decreased the luciferase reporter activity of Atg7. Further, inflammatory cytokine levels in murine lung epithelial cell line cells were downregulated following autophagy induction by starvation and rapamycin treatment, and upregulated when autophagy was blocked using 3-methyladenine and chloroquine. atg7 knockout mice and Atg7 small interfering RNA-treated cells exhibited elevated levels of phospho-NF-κB and intensified inflammatory cytokines, suggesting that Atg7 impacts inflammatory responses to hyperoxia. These findings demonstrate that OGG-1 negatively regulates inflammatory cytokine release by coordinating molecular interaction with the autophagic pathway in hyperoxia-induced lung injury.
DOI: 10.4049/jimmunol.1601001
PubMed: 28202616
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pubmed:28202616Le document en format XML
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<term>Acute Lung Injury (pathology)</term>
<term>Animals</term>
<term>Autophagy</term>
<term>Blotting, Western</term>
<term>Chromatin Immunoprecipitation</term>
<term>Comet Assay</term>
<term>Cytokines (biosynthesis)</term>
<term>DNA Glycosylases (metabolism)</term>
<term>DNA Repair</term>
<term>Disease Models, Animal</term>
<term>Hyperoxia (metabolism)</term>
<term>Hyperoxia (pathology)</term>
<term>Immunoprecipitation</term>
<term>Inflammation (metabolism)</term>
<term>Inflammation (pathology)</term>
<term>Lung (metabolism)</term>
<term>Lung (pathology)</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Mice, Knockout</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Autophagie</term>
<term>Cytokines (biosynthèse)</term>
<term>DNA Glycosylases (métabolisme)</term>
<term>Hyperoxie (anatomopathologie)</term>
<term>Hyperoxie (métabolisme)</term>
<term>Immunoprécipitation</term>
<term>Immunoprécipitation de la chromatine</term>
<term>Inflammation (anatomopathologie)</term>
<term>Inflammation (métabolisme)</term>
<term>Lésion pulmonaire aigüe (anatomopathologie)</term>
<term>Lésion pulmonaire aigüe (métabolisme)</term>
<term>Modèles animaux de maladie humaine</term>
<term>Poumon (anatomopathologie)</term>
<term>Poumon (métabolisme)</term>
<term>Réparation de l'ADN</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Souris knockout</term>
<term>Technique de Western</term>
<term>Test des comètes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cytokines</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Hyperoxie</term>
<term>Inflammation</term>
<term>Lésion pulmonaire aigüe</term>
<term>Poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cytokines</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Acute Lung Injury</term>
<term>DNA Glycosylases</term>
<term>Hyperoxia</term>
<term>Inflammation</term>
<term>Lung</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>DNA Glycosylases</term>
<term>Hyperoxie</term>
<term>Inflammation</term>
<term>Lésion pulmonaire aigüe</term>
<term>Poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Acute Lung Injury</term>
<term>Hyperoxia</term>
<term>Inflammation</term>
<term>Lung</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Autophagy</term>
<term>Blotting, Western</term>
<term>Chromatin Immunoprecipitation</term>
<term>Comet Assay</term>
<term>DNA Repair</term>
<term>Disease Models, Animal</term>
<term>Immunoprecipitation</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Mice, Knockout</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Autophagie</term>
<term>Immunoprécipitation</term>
<term>Immunoprécipitation de la chromatine</term>
<term>Modèles animaux de maladie humaine</term>
<term>Réparation de l'ADN</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Souris knockout</term>
<term>Technique de Western</term>
<term>Test des comètes</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Oxygen is supplied as a supportive treatment for patients suffering from acute respiratory distress syndrome. Unfortunately, high oxygen concentration increases reactive oxygen species generation, which causes DNA damage and ultimately cell death in the lung. Although 8-oxoguanine-DNA glycosylase (OGG-1) is involved in repairing hyperoxia-mediated DNA damage, the underlying molecular mechanism remains elusive. In this study, we report that <i>ogg-1</i>
-deficient mice exhibited a significant increase of proinflammatory cytokines (TNF-α, IL-6, and IFN-γ) in the lung after being exposed to 95% oxygen. In addition, we found that <i>ogg-1</i>
deficiency downregulated (macro)autophagy when exposed to hyperoxia both in vitro and in vivo, which was evident by decreased conversion of LC3-I to LC3-II, reduced LC3 punctate staining, and lower Atg7 expression compared with controls. Using a chromatin immunoprecipitation assay, we found that OGG-1 associated with the promoter of Atg7, suggesting a role for OGG1 in regulation of Atg7 activity. Knocking down OGG-1 decreased the luciferase reporter activity of Atg7. Further, inflammatory cytokine levels in murine lung epithelial cell line cells were downregulated following autophagy induction by starvation and rapamycin treatment, and upregulated when autophagy was blocked using 3-methyladenine and chloroquine. <i>atg7</i>
knockout mice and Atg7 small interfering RNA-treated cells exhibited elevated levels of phospho-NF-κB and intensified inflammatory cytokines, suggesting that Atg7 impacts inflammatory responses to hyperoxia. These findings demonstrate that OGG-1 negatively regulates inflammatory cytokine release by coordinating molecular interaction with the autophagic pathway in hyperoxia-induced lung injury.</div>
</front>
</TEI>
</record>
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