Merge
Accueil
Racine
Merge
Exploration
Accueil
Racine
Accueil
Racine

Serveur d'exploration Chloroquine

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Inhibition LC3B can increase chemosensitivity of ovarian cancer cells

Identifieur interne : 000731 ( Main/Merge ); précédent : 000730; suivant : 000732

Inhibition LC3B can increase chemosensitivity of ovarian cancer cells

Auteurs : Jing Tang [République populaire de Chine] ; Jiang Zhu [République populaire de Chine] ; Yuguang Ye [République populaire de Chine] ; Yu Liu [République populaire de Chine] ; Yan He [République populaire de Chine] ; Lei Zhang [République populaire de Chine] ; Dai Tang [République populaire de Chine] ; Cong Qiao [République populaire de Chine] ; Xinxin Feng [République populaire de Chine] ; Junyi Li [République populaire de Chine] ; Yanni Kan [République populaire de Chine] ; Xiaobo Li [République populaire de Chine] ; Xiaoming Jin [République populaire de Chine] ; Dan Kong [République populaire de Chine]

Source :

RBID : PMC:6664537

Abstract

Background

Ovarian cancer is often accompanied by the production of ascites, and patients with repeated ascites are associated with chemotherapy resistance. The previous study confirmed that the ovarian cancer patients who developed ascites after chemotherapy had elevated autophagy levels in the ascites and precipitated cells, which was positively correlated with MDR1 expression in the blood of patients.

Methods

In order to explore the correlation between autophagy and chemoresistant, we searched TCGA and GEO database to analyze the correlation between LC3B and MDR1, and identified the targeting miRNA of LC3B. It was verified by dual luciferase that miR-204 can target LC3B. The ovarian cancer cell line and the BALB/c nude mice tumor-bearing model were selected for in vitro and in vivo verification. In vitro studies confirmed that ovarian cancer cells were more sensitive to cisplatin by inhibiting LC3B.

Results

Overexpression of miR-204 reduced the expression of LC3B, Atg7, and MDR1, and promoted apoptosis. In vivo studies have also confirmed that reducing the level of autophagy in ovarian cancer cells increases the sensitivity to cisplatin.

Conclusions

It suggests that miR-204 can be used as a tumor suppressor gene and LC3B expression level can be used as a potential molecular marker to guide the diagnosis and treatment of patients with ovarian cancer.

Electronic supplementary material

The online version of this article (10.1186/s12935-019-0921-z) contains supplementary material, which is available to authorized users.


Url:
DOI: 10.1186/s12935-019-0921-z
PubMed: 31384174
PubMed Central: 6664537

Links toward previous steps (curation, corpus...)

Links to Exploration step

PMC:6664537

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Inhibition LC3B can increase chemosensitivity of ovarian cancer cells</title>
<author>
<name sortKey="Tang, Jing" sort="Tang, Jing" uniqKey="Tang J" first="Jing" last="Tang">Jing Tang</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff1">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0000 8877 7471</institution-id>
<institution-id institution-id-type="GRID">grid.284723.8</institution-id>
<institution>Department of Bioinformatics,</institution>
<institution>Southern Medical University,</institution>
</institution-wrap>
Guangzhou, 510515 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangzhou</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Zhu, Jiang" sort="Zhu, Jiang" uniqKey="Zhu J" first="Jiang" last="Zhu">Jiang Zhu</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff3">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0004 1797 9737</institution-id>
<institution-id institution-id-type="GRID">grid.412596.d</institution-id>
<institution>Department of Orthopedics,</institution>
<institution>First Affiliated Hospital of Harbin Medical University,</institution>
</institution-wrap>
Harbin, 150001 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Harbin</wicri:regionArea>
</affiliation>
</author>
<author>
<name sortKey="Ye, Yuguang" sort="Ye, Yuguang" uniqKey="Ye Y" first="Yuguang" last="Ye">Yuguang Ye</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff4">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0004 1808 3502</institution-id>
<institution-id institution-id-type="GRID">grid.412651.5</institution-id>
<institution>Department of Gynecology,</institution>
<institution>Harbin Medical University Cancer Hospital,</institution>
</institution-wrap>
No. 150 Haping Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 150 Haping Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Liu, Yu" sort="Liu, Yu" uniqKey="Liu Y" first="Yu" last="Liu">Yu Liu</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="He, Yan" sort="He, Yan" uniqKey="He Y" first="Yan" last="He">Yan He</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Tang, Dai" sort="Tang, Dai" uniqKey="Tang D" first="Dai" last="Tang">Dai Tang</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Qiao, Cong" sort="Qiao, Cong" uniqKey="Qiao C" first="Cong" last="Qiao">Cong Qiao</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Feng, Xinxin" sort="Feng, Xinxin" uniqKey="Feng X" first="Xinxin" last="Feng">Xinxin Feng</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Li, Junyi" sort="Li, Junyi" uniqKey="Li J" first="Junyi" last="Li">Junyi Li</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Kan, Yanni" sort="Kan, Yanni" uniqKey="Kan Y" first="Yanni" last="Kan">Yanni Kan</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Li, Xiaobo" sort="Li, Xiaobo" uniqKey="Li X" first="Xiaobo" last="Li">Xiaobo Li</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Jin, Xiaoming" sort="Jin, Xiaoming" uniqKey="Jin X" first="Xiaoming" last="Jin">Xiaoming Jin</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Kong, Dan" sort="Kong, Dan" uniqKey="Kong D" first="Dan" last="Kong">Dan Kong</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff4">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0004 1808 3502</institution-id>
<institution-id institution-id-type="GRID">grid.412651.5</institution-id>
<institution>Department of Gynecology,</institution>
<institution>Harbin Medical University Cancer Hospital,</institution>
</institution-wrap>
No. 150 Haping Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 150 Haping Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">PMC</idno>
<idno type="pmid">31384174</idno>
<idno type="pmc">6664537</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664537</idno>
<idno type="RBID">PMC:6664537</idno>
<idno type="doi">10.1186/s12935-019-0921-z</idno>
<date when="2019">2019</date>
<idno type="wicri:Area/Pmc/Corpus">000051</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000051</idno>
<idno type="wicri:Area/Pmc/Curation">000051</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000051</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000676</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000676</idno>
<idno type="wicri:Area/Ncbi/Merge">000886</idno>
<idno type="wicri:Area/Ncbi/Curation">000886</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000886</idno>
<idno type="wicri:Area/Main/Merge">000731</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en" level="a" type="main">Inhibition LC3B can increase chemosensitivity of ovarian cancer cells</title>
<author>
<name sortKey="Tang, Jing" sort="Tang, Jing" uniqKey="Tang J" first="Jing" last="Tang">Jing Tang</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff1">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0000 8877 7471</institution-id>
<institution-id institution-id-type="GRID">grid.284723.8</institution-id>
<institution>Department of Bioinformatics,</institution>
<institution>Southern Medical University,</institution>
</institution-wrap>
Guangzhou, 510515 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangzhou</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Zhu, Jiang" sort="Zhu, Jiang" uniqKey="Zhu J" first="Jiang" last="Zhu">Jiang Zhu</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff3">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0004 1797 9737</institution-id>
<institution-id institution-id-type="GRID">grid.412596.d</institution-id>
<institution>Department of Orthopedics,</institution>
<institution>First Affiliated Hospital of Harbin Medical University,</institution>
</institution-wrap>
Harbin, 150001 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Harbin</wicri:regionArea>
</affiliation>
</author>
<author>
<name sortKey="Ye, Yuguang" sort="Ye, Yuguang" uniqKey="Ye Y" first="Yuguang" last="Ye">Yuguang Ye</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff4">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0004 1808 3502</institution-id>
<institution-id institution-id-type="GRID">grid.412651.5</institution-id>
<institution>Department of Gynecology,</institution>
<institution>Harbin Medical University Cancer Hospital,</institution>
</institution-wrap>
No. 150 Haping Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 150 Haping Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Liu, Yu" sort="Liu, Yu" uniqKey="Liu Y" first="Yu" last="Liu">Yu Liu</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="He, Yan" sort="He, Yan" uniqKey="He Y" first="Yan" last="He">Yan He</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Tang, Dai" sort="Tang, Dai" uniqKey="Tang D" first="Dai" last="Tang">Dai Tang</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Qiao, Cong" sort="Qiao, Cong" uniqKey="Qiao C" first="Cong" last="Qiao">Cong Qiao</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Feng, Xinxin" sort="Feng, Xinxin" uniqKey="Feng X" first="Xinxin" last="Feng">Xinxin Feng</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Li, Junyi" sort="Li, Junyi" uniqKey="Li J" first="Junyi" last="Li">Junyi Li</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Kan, Yanni" sort="Kan, Yanni" uniqKey="Kan Y" first="Yanni" last="Kan">Yanni Kan</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Li, Xiaobo" sort="Li, Xiaobo" uniqKey="Li X" first="Xiaobo" last="Li">Xiaobo Li</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Jin, Xiaoming" sort="Jin, Xiaoming" uniqKey="Jin X" first="Xiaoming" last="Jin">Xiaoming Jin</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff2">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0001 2204 9268</institution-id>
<institution-id institution-id-type="GRID">grid.410736.7</institution-id>
<institution>Department of Pathology,</institution>
<institution>Harbin Medical University,</institution>
</institution-wrap>
No. 157 Baojian Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 157 Baojian Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Kong, Dan" sort="Kong, Dan" uniqKey="Kong D" first="Dan" last="Kong">Dan Kong</name>
<affiliation wicri:level="1">
<nlm:aff id="Aff4">
<institution-wrap>
<institution-id institution-id-type="ISNI">0000 0004 1808 3502</institution-id>
<institution-id institution-id-type="GRID">grid.412651.5</institution-id>
<institution>Department of Gynecology,</institution>
<institution>Harbin Medical University Cancer Hospital,</institution>
</institution-wrap>
No. 150 Haping Road, Nangang District, Harbin, 150081 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>No. 150 Haping Road, Nangang District, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series>
<title level="j">Cancer Cell International</title>
<idno type="eISSN">1475-2867</idno>
<imprint>
<date when="2019">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass></textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">
<sec>
<title>Background</title>
<p id="Par1">Ovarian cancer is often accompanied by the production of ascites, and patients with repeated ascites are associated with chemotherapy resistance. The previous study confirmed that the ovarian cancer patients who developed ascites after chemotherapy had elevated autophagy levels in the ascites and precipitated cells, which was positively correlated with MDR1 expression in the blood of patients.</p>
</sec>
<sec>
<title>Methods</title>
<p id="Par2">In order to explore the correlation between autophagy and chemoresistant, we searched TCGA and GEO database to analyze the correlation between LC3B and MDR1, and identified the targeting miRNA of LC3B. It was verified by dual luciferase that miR-204 can target LC3B. The ovarian cancer cell line and the BALB/c nude mice tumor-bearing model were selected for in vitro and in vivo verification. In vitro studies confirmed that ovarian cancer cells were more sensitive to cisplatin by inhibiting LC3B.</p>
</sec>
<sec>
<title>Results</title>
<p id="Par3">Overexpression of miR-204 reduced the expression of LC3B, Atg7, and MDR1, and promoted apoptosis. In vivo studies have also confirmed that reducing the level of autophagy in ovarian cancer cells increases the sensitivity to cisplatin.</p>
</sec>
<sec>
<title>Conclusions</title>
<p id="Par4">It suggests that miR-204 can be used as a tumor suppressor gene and LC3B expression level can be used as a potential molecular marker to guide the diagnosis and treatment of patients with ovarian cancer.</p>
</sec>
<sec>
<title>Electronic supplementary material</title>
<p>The online version of this article (10.1186/s12935-019-0921-z) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
<back>
<div1 type="bibliography">
<listBibl>
<biblStruct>
<analytic>
<author>
<name sortKey="Jayson, Gc" uniqKey="Jayson G">GC Jayson</name>
</author>
<author>
<name sortKey="Kohn, Ec" uniqKey="Kohn E">EC Kohn</name>
</author>
<author>
<name sortKey="Kitchener, Hc" uniqKey="Kitchener H">HC Kitchener</name>
</author>
<author>
<name sortKey="Ledermann, Ja" uniqKey="Ledermann J">JA Ledermann</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Jelovac, D" uniqKey="Jelovac D">D Jelovac</name>
</author>
<author>
<name sortKey="Armstrong, Dk" uniqKey="Armstrong D">DK Armstrong</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Pignata, S" uniqKey="Pignata S">S Pignata</name>
</author>
<author>
<name sortKey="Cecere, S" uniqKey="Cecere S">S Cecere</name>
</author>
<author>
<name sortKey="Du Bois, A" uniqKey="Du Bois A">A Du Bois</name>
</author>
<author>
<name sortKey="Harter, P" uniqKey="Harter P">P Harter</name>
</author>
<author>
<name sortKey="Heitz, F" uniqKey="Heitz F">F Heitz</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Morgan, Sl" uniqKey="Morgan S">SL Morgan</name>
</author>
<author>
<name sortKey="Medina, Je" uniqKey="Medina J">JE Medina</name>
</author>
<author>
<name sortKey="Taylor, Mm" uniqKey="Taylor M">MM Taylor</name>
</author>
<author>
<name sortKey="Dinulescu, Dm" uniqKey="Dinulescu D">DM Dinulescu</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Liu, Yu" uniqKey="Liu Y">Yu Liu</name>
</author>
<author>
<name sortKey="Tang, Jing" uniqKey="Tang J">Jing Tang</name>
</author>
<author>
<name sortKey="Liu, Duanyang" uniqKey="Liu D">Duanyang Liu</name>
</author>
<author>
<name sortKey="Zhang, Lei" uniqKey="Zhang L">Lei Zhang</name>
</author>
<author>
<name sortKey="He, Yan" uniqKey="He Y">Yan He</name>
</author>
<author>
<name sortKey="Li, Jing" uniqKey="Li J">Jing Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Levy, Jmm" uniqKey="Levy J">JMM Levy</name>
</author>
<author>
<name sortKey="Towers, Cg" uniqKey="Towers C">CG Towers</name>
</author>
<author>
<name sortKey="Thorburn, A" uniqKey="Thorburn A">A Thorburn</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Kenific, Cm" uniqKey="Kenific C">CM Kenific</name>
</author>
<author>
<name sortKey="Debnath, J" uniqKey="Debnath J">J Debnath</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="White, E" uniqKey="White E">E White</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Wang, J" uniqKey="Wang J">J Wang</name>
</author>
<author>
<name sortKey="Wu, Gs" uniqKey="Wu G">GS Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Hou, Yj" uniqKey="Hou Y">YJ Hou</name>
</author>
<author>
<name sortKey="Dong, Lw" uniqKey="Dong L">LW Dong</name>
</author>
<author>
<name sortKey="Tan, Yx" uniqKey="Tan Y">YX Tan</name>
</author>
<author>
<name sortKey="Yang, Gz" uniqKey="Yang G">GZ Yang</name>
</author>
<author>
<name sortKey="Pan, Yf" uniqKey="Pan Y">YF Pan</name>
</author>
<author>
<name sortKey="Li, Z" uniqKey="Li Z">Z Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Schaaf, Mb" uniqKey="Schaaf M">MB Schaaf</name>
</author>
<author>
<name sortKey="Keulers, Tg" uniqKey="Keulers T">TG Keulers</name>
</author>
<author>
<name sortKey="Vooijs, Ma" uniqKey="Vooijs M">MA Vooijs</name>
</author>
<author>
<name sortKey="Rouschop, Km" uniqKey="Rouschop K">KM Rouschop</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Lee, Sj" uniqKey="Lee S">SJ Lee</name>
</author>
<author>
<name sortKey="Smith, A" uniqKey="Smith A">A Smith</name>
</author>
<author>
<name sortKey="Guo, L" uniqKey="Guo L">L Guo</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Hurley, Jh" uniqKey="Hurley J">JH Hurley</name>
</author>
<author>
<name sortKey="Young, Ln" uniqKey="Young L">LN Young</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Morgan, Rj" uniqKey="Morgan R">RJ Morgan</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Kipps, E" uniqKey="Kipps E">E Kipps</name>
</author>
<author>
<name sortKey="Tan, Ds" uniqKey="Tan D">DS Tan</name>
</author>
<author>
<name sortKey="Kaye, Sb" uniqKey="Kaye S">SB Kaye</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Mikhaylova, O" uniqKey="Mikhaylova O">O Mikhaylova</name>
</author>
<author>
<name sortKey="Stratton, Y" uniqKey="Stratton Y">Y Stratton</name>
</author>
<author>
<name sortKey="Hall, D" uniqKey="Hall D">D Hall</name>
</author>
<author>
<name sortKey="Kellner, E" uniqKey="Kellner E">E Kellner</name>
</author>
<author>
<name sortKey="Ehmer, B" uniqKey="Ehmer B">B Ehmer</name>
</author>
<author>
<name sortKey="Drew, Af" uniqKey="Drew A">AF Drew</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Klionsky, Dj" uniqKey="Klionsky D">DJ Klionsky</name>
</author>
<author>
<name sortKey="Abdelmohsen, K" uniqKey="Abdelmohsen K">K Abdelmohsen</name>
</author>
<author>
<name sortKey="Abe, A" uniqKey="Abe A">A Abe</name>
</author>
<author>
<name sortKey="Abedin, Mj" uniqKey="Abedin M">MJ Abedin</name>
</author>
<author>
<name sortKey="Abeliovich, H" uniqKey="Abeliovich H">H Abeliovich</name>
</author>
<author>
<name sortKey="Acevedo Arozena, A" uniqKey="Acevedo Arozena A">A Acevedo Arozena</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Loos, B" uniqKey="Loos B">B Loos</name>
</author>
<author>
<name sortKey="Du Toit, A" uniqKey="Du Toit A">A du Toit</name>
</author>
<author>
<name sortKey="Hofmeyr, Jh" uniqKey="Hofmeyr J">JH Hofmeyr</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Yoshida, Gj" uniqKey="Yoshida G">GJ Yoshida</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Sacconi, A" uniqKey="Sacconi A">A Sacconi</name>
</author>
<author>
<name sortKey="Biagioni, F" uniqKey="Biagioni F">F Biagioni</name>
</author>
<author>
<name sortKey="Canu, V" uniqKey="Canu V">V Canu</name>
</author>
<author>
<name sortKey="Mori, F" uniqKey="Mori F">F Mori</name>
</author>
<author>
<name sortKey="Di Benedetto, A" uniqKey="Di Benedetto A">A Di Benedetto</name>
</author>
<author>
<name sortKey="Lorenzon, L" uniqKey="Lorenzon L">L Lorenzon</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Canu, V" uniqKey="Canu V">V Canu</name>
</author>
<author>
<name sortKey="Sacconi, A" uniqKey="Sacconi A">A Sacconi</name>
</author>
<author>
<name sortKey="Lorenzon, L" uniqKey="Lorenzon L">L Lorenzon</name>
</author>
<author>
<name sortKey="Biagioni, F" uniqKey="Biagioni F">F Biagioni</name>
</author>
<author>
<name sortKey="Lo Sardo, F" uniqKey="Lo Sardo F">F Lo Sardo</name>
</author>
<author>
<name sortKey="Diodoro, Mg" uniqKey="Diodoro M">MG Diodoro</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Zhu, H" uniqKey="Zhu H">H Zhu</name>
</author>
<author>
<name sortKey="Wu, H" uniqKey="Wu H">H Wu</name>
</author>
<author>
<name sortKey="Liu, X" uniqKey="Liu X">X Liu</name>
</author>
<author>
<name sortKey="Li, B" uniqKey="Li B">B Li</name>
</author>
<author>
<name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name>
</author>
<author>
<name sortKey="Ren, X" uniqKey="Ren X">X Ren</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Janku, F" uniqKey="Janku F">F Janku</name>
</author>
<author>
<name sortKey="Mcconkey, Dj" uniqKey="Mcconkey D">DJ McConkey</name>
</author>
<author>
<name sortKey="Hong, Ds" uniqKey="Hong D">DS Hong</name>
</author>
<author>
<name sortKey="Kurzrock, R" uniqKey="Kurzrock R">R Kurzrock</name>
</author>
</analytic>
</biblStruct>
<biblStruct>
<analytic>
<author>
<name sortKey="Yun, Cw" uniqKey="Yun C">CW Yun</name>
</author>
<author>
<name sortKey="Lee, Sh" uniqKey="Lee S">SH Lee</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000731 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000731 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    ChloroquineV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     PMC:6664537
   |texte=   Inhibition LC3B can increase chemosensitivity of ovarian cancer cells
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i   -Sk "pubmed:31384174" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a ChloroquineV1
Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Wed Mar 25 22:43:59 2020. Site generation: Sun Jan 31 12:44:45 2021