Entry of African swine fever virus into Vero cells and uncoating
Identifieur interne : 002761 ( Main/Curation ); précédent : 002760; suivant : 002762Entry of African swine fever virus into Vero cells and uncoating
Auteurs : M. Luisa Valdeira [Portugal] ; Catarina Bernardes [Portugal] ; B. Cruz [Portugal] ; A. Geraldes [Portugal]Source :
- Veterinary Microbiology [ 0378-1135 ] ; 1998.
English descriptors
- Teeft :
- African swine fever virus, Ammonium, Ammonium chloride, Aprotinin, Asfv, Asfv entry, Asfv infection, Asfv penetration, Bovine pancreas, Cell biol, Cellular biology, Chloromethyl ketone, Electron microscopy, Endocytosis, Endosomal membranes, Forcas armadas, Geraldes, Inhibitor, Iodoacetic acid, Leupeptin, Lysosomotropic, Lysosomotropic agents, Lysosomotropic drugs, Manso ribeiro, Microbiology, Monensin, Morphological study, Normal medium, Other authors, Oxidative phosphorylation, Penetration period, Plaque titration, Plasma membrane, Pmsf, Protease, Protease inhibitor, Protease inhibitors, Proteolytic activation, Proteolytic cleavage, Rapid reversion, Rosa azevedo, Sodium azide, Specific activity cirmmol, Stock solutions, Swine, Vaccinia virus, Valdeira, Vero, Vero cells, Veterinary microbiology, Viral, Viral adsorption, Viral entry, Viral envelope, Viral membrane, Viral particles, Viral uncoating, Virion, Virol, Virus, Virus uptake.
Abstract
Abstract: African swine fever virus (ASFV) enters Vero cells by adsorptive endocytosis [Valdeira, M.L., Geraldes, A., 1985. Morphological study on the entry of African swine fever virus into cells, Biol. Cell. 55, 35–40]. Electron microscopy of a lysosomotropic drug-controlled penetration indicated that this step takes place in the endosomes, after fusion between the viral envelope and the limiting membrane of the endosome. Inhibition studies with colcemid, cytochalasin B, sodium azide, dinitrophenol, lysosomotropic weak bases, and the ionophore monensin, showed that the virus uptake is largely independent of cytoskeletal and lysosomal function, but dependent on oxidative phosphorylation. Some protease inhibitors inhibited viral replication at an early step, indicating that the initiation of infection depends on a viral proteolytic cleavage.
Url:
DOI: 10.1016/S0378-1135(98)00152-7
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Luisa" last="Valdeira">M. Luisa Valdeira</name><affiliation wicri:level="1"><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Cellular Biology, Faculty of Pharmacy, Avenida das Forças Armadas, 1600 Lisboa</wicri:regionArea><wicri:noRegion>1600 Lisboa</wicri:noRegion></affiliation></author><author><name sortKey="Bernardes, Catarina" sort="Bernardes, Catarina" uniqKey="Bernardes C" first="Catarina" last="Bernardes">Catarina Bernardes</name><affiliation wicri:level="1"><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Cellular Biology, Faculty of Pharmacy, Avenida das Forças Armadas, 1600 Lisboa</wicri:regionArea><wicri:noRegion>1600 Lisboa</wicri:noRegion></affiliation></author><author><name sortKey="Cruz, B" sort="Cruz, B" uniqKey="Cruz B" first="B" last="Cruz">B. 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Geraldes</name><affiliation wicri:level="1"><country xml:lang="fr">Portugal</country><wicri:regionArea>Virology Laboratory, Gulbenkian Institute of Science, Apartado 14, 2781 Oeiras Codex</wicri:regionArea><wicri:noRegion>2781 Oeiras Codex</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Veterinary Microbiology</title><title level="j" type="abbrev">VETMIC</title><idno type="ISSN">0378-1135</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">60</biblScope><biblScope unit="issue">2–4</biblScope><biblScope unit="page" from="131">131</biblScope><biblScope unit="page" to="140">140</biblScope></imprint><idno type="ISSN">0378-1135</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1135</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>African swine fever virus</term><term>Ammonium</term><term>Ammonium chloride</term><term>Aprotinin</term><term>Asfv</term><term>Asfv entry</term><term>Asfv infection</term><term>Asfv penetration</term><term>Bovine pancreas</term><term>Cell biol</term><term>Cellular biology</term><term>Chloromethyl ketone</term><term>Electron microscopy</term><term>Endocytosis</term><term>Endosomal membranes</term><term>Forcas armadas</term><term>Geraldes</term><term>Inhibitor</term><term>Iodoacetic acid</term><term>Leupeptin</term><term>Lysosomotropic</term><term>Lysosomotropic agents</term><term>Lysosomotropic drugs</term><term>Manso ribeiro</term><term>Microbiology</term><term>Monensin</term><term>Morphological study</term><term>Normal medium</term><term>Other authors</term><term>Oxidative phosphorylation</term><term>Penetration period</term><term>Plaque titration</term><term>Plasma membrane</term><term>Pmsf</term><term>Protease</term><term>Protease inhibitor</term><term>Protease inhibitors</term><term>Proteolytic activation</term><term>Proteolytic cleavage</term><term>Rapid reversion</term><term>Rosa azevedo</term><term>Sodium azide</term><term>Specific activity cirmmol</term><term>Stock solutions</term><term>Swine</term><term>Vaccinia virus</term><term>Valdeira</term><term>Vero</term><term>Vero cells</term><term>Veterinary microbiology</term><term>Viral</term><term>Viral adsorption</term><term>Viral entry</term><term>Viral envelope</term><term>Viral membrane</term><term>Viral particles</term><term>Viral uncoating</term><term>Virion</term><term>Virol</term><term>Virus</term><term>Virus uptake</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: African swine fever virus (ASFV) enters Vero cells by adsorptive endocytosis [Valdeira, M.L., Geraldes, A., 1985. Morphological study on the entry of African swine fever virus into cells, Biol. Cell. 55, 35–40]. Electron microscopy of a lysosomotropic drug-controlled penetration indicated that this step takes place in the endosomes, after fusion between the viral envelope and the limiting membrane of the endosome. Inhibition studies with colcemid, cytochalasin B, sodium azide, dinitrophenol, lysosomotropic weak bases, and the ionophore monensin, showed that the virus uptake is largely independent of cytoskeletal and lysosomal function, but dependent on oxidative phosphorylation. Some protease inhibitors inhibited viral replication at an early step, indicating that the initiation of infection depends on a viral proteolytic cleavage.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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