Molecular epidemiology of drug-resistant Plasmodium falciparum in Benguela province, Angola
Identifieur interne : 000E52 ( Main/Curation ); précédent : 000E51; suivant : 000E53Molecular epidemiology of drug-resistant Plasmodium falciparum in Benguela province, Angola
Auteurs : Vincent Foumane Ngane [Cameroun] ; Joseph Allico Djaman [Côte d'Ivoire] ; Cécile Culeux [France] ; Nathalie Piette [France] ; Pierre Carnevale [Angola] ; Patrick Besnard [Angola] ; Filomeno Fortes [Angola] ; Leonardo K. Basco [France] ; Rachida Tahar [France]Source :
- Malaria Journal [ 1475-2875 ] ; 2015-03-14.
English descriptors
- mix :
Abstract
Background: The malaria situation has been worsening in Angola, partly due to armed conflict until the recent past and drug-resistant Plasmodium falciparum. Malaria transmission is heterogeneous within the country, and data on drug-resistant malaria in different parts of the country are incomplete. The aim of the present study was to evaluate resistance to 4-aminoquinolines and antifolate drugs in P. falciparum isolates collected in Benguela province, central Angola, using molecular markers. Methods: Fingerprick capillary blood was collected from asymptomatic children aged less than 15 years old during a household survey in and around Balombo town in 2010–2011. Samples were screened for P. falciparum by nested PCR. Molecular markers (P. falciparum dihydrofolate reductase [pfdhfr], P. falciparum dihydropteroate synthase [pfdhps], P. falciparum chloroquine resistance transporter [pfcrt], and P. falciparum multidrug-resistance gene 1 [pfmdr1]) were sequenced to determine the key codons associated with drug resistance. Results: A total of 60 blood samples were positive for P. falciparum. Most isolates with successful PCR amplification had mutant pfdhfr alleles, with either double mutant AICNI (69%) or triple mutant AIRNI (21%) haplotypes. A16V, S108T, and I164L substitutions were not found. Many of the isolates were carriers of either SGKAA (60%) or AGKAA (27%) pfdhps haplotype. K540E substitution was absent. There were only two pfcrt haplotypes: wild-type CVMNK (11%) and mutant CVIET (89%). Wild-type pfmdr1 NYSND haplotype was found in 19% of the isolates, whereas single mutant pfmdr1 YYSND and NFSND haplotypes occurred in 48% and 11%, respectively. Double mutant pfmdr1 haplotypes (YFSND and YYSNY) occurred rarely. Conclusions: The results suggest that the high prevalence of mutant pfcrt CVIET haplotype is in agreement with low clinical efficacy of chloroquine observed in earlier studies and that the double pfdhfr mutant AICNI and single pfdhps mutant SGKAA are currently the predominant haplotypes associated with antifolate resistance in Benguela province. The hallmark of clinical resistance observed in East Africa, i.e. triple pfdhfr mutant haplotype (AIRNI) and double pfdhps mutant haplotype (SGEAA), was absent. These molecular findings need to be further evaluated in parallel with clinical studies, in particular with the efficacy of intermittent preventive treatment using sulphadoxine-pyrimethamine in pregnant women and artesunate-amodiaquine for uncomplicated malaria.
Url:
DOI: 10.1186/s12936-015-0634-2
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000163
- to stream Hal, to step Curation: Pour aller vers cette notice dans l'étape Curation :000163
- to stream Hal, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000099
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :000E53
Links to Exploration step
Hal:hal-01217921Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular epidemiology of drug-resistant Plasmodium falciparum in Benguela province, Angola</title>
<author><name sortKey="Foumane Ngane, Vincent" sort="Foumane Ngane, Vincent" uniqKey="Foumane Ngane V" first="Vincent" last="Foumane Ngane">Vincent Foumane Ngane</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-81274" status="INCOMING"> <orgName>Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale</orgName>
<orgName type="acronym">OCEAC</orgName>
<desc> <address> <addrLine>BP 288, Yaoundé, Cameroon</addrLine>
<country key="CM"></country>
</address>
</desc>
<listRelation> <relation active="#struct-320801" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-320801" type="direct"><org type="institution" xml:id="struct-320801" status="INCOMING"> <orgName>Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Cameroun</country>
</affiliation>
</author>
<author><name sortKey="Djaman, Joseph Allico" sort="Djaman, Joseph Allico" uniqKey="Djaman J" first="Joseph Allico" last="Djaman">Joseph Allico Djaman</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-242267" status="VALID"> <orgName>Laboratoire de Pharmacodynamie Biochimique</orgName>
<desc> <address> <addrLine>01 BP V34 Abidjan 01</addrLine>
<country key="CI"></country>
</address>
</desc>
<listRelation> <relation active="#struct-218070" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-218070" type="direct"><org type="institution" xml:id="struct-218070" status="VALID"> <orgName>Université Félix Houphouët-Boigny</orgName>
<orgName type="acronym">UFHB</orgName>
<desc> <address> <addrLine> Abidjan</addrLine>
<country key="CI"></country>
</address>
<ref type="url">http://univ-fhb.edu.ci/fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Côte d'Ivoire</country>
</affiliation>
</author>
<author><name sortKey="Culeux, Cecile" sort="Culeux, Cecile" uniqKey="Culeux C" first="Cécile" last="Culeux">Cécile Culeux</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-134260" status="OLD"> <idno type="IdRef">180320637</idno>
<idno type="ISNI">0000 0004 0508 7272 </idno>
<idno type="RNSR">201020824W</idno>
<orgName>Mère et enfant face aux infections tropicales</orgName>
<orgName type="acronym">MERIT - UMR_D 216</orgName>
<date type="start">2014-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté de pharmacie - Laboratoire de parasitologie4 avenue de l'Observatoire 75270 Paris cedex 6</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umr216.fr</ref>
</desc>
<listRelation> <relation active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 216" active="#struct-301664" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 216" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD"> <idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<date type="start">1971-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Piette, Nathalie" sort="Piette, Nathalie" uniqKey="Piette N" first="Nathalie" last="Piette">Nathalie Piette</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-134260" status="OLD"> <idno type="IdRef">180320637</idno>
<idno type="ISNI">0000 0004 0508 7272 </idno>
<idno type="RNSR">201020824W</idno>
<orgName>Mère et enfant face aux infections tropicales</orgName>
<orgName type="acronym">MERIT - UMR_D 216</orgName>
<date type="start">2014-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté de pharmacie - Laboratoire de parasitologie4 avenue de l'Observatoire 75270 Paris cedex 6</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umr216.fr</ref>
</desc>
<listRelation> <relation active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 216" active="#struct-301664" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 216" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD"> <idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<date type="start">1971-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Carnevale, Pierre" sort="Carnevale, Pierre" uniqKey="Carnevale P" first="Pierre" last="Carnevale">Pierre Carnevale</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439701" status="INCOMING"> <orgName>Malaria Control Programme</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
<listRelation> <relation active="#struct-348140" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-348140" type="direct"><org type="institution" xml:id="struct-348140" status="INCOMING"> <orgName>Société Nationale de Métallurgie (Sonamet)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Angola</country>
</affiliation>
</author>
<author><name sortKey="Besnard, Patrick" sort="Besnard, Patrick" uniqKey="Besnard P" first="Patrick" last="Besnard">Patrick Besnard</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439701" status="INCOMING"> <orgName>Malaria Control Programme</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
<listRelation> <relation active="#struct-348140" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-348140" type="direct"><org type="institution" xml:id="struct-348140" status="INCOMING"> <orgName>Société Nationale de Métallurgie (Sonamet)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Angola</country>
</affiliation>
</author>
<author><name sortKey="Fortes, Filomeno" sort="Fortes, Filomeno" uniqKey="Fortes F" first="Filomeno" last="Fortes">Filomeno Fortes</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439703" status="INCOMING"> <orgName>Plano National Contra Malaria</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
<listRelation> <relation active="#struct-439702" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-439702" type="direct"><org type="institution" xml:id="struct-439702" status="INCOMING"> <orgName>Ministry of Health</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Angola</country>
</affiliation>
</author>
<author><name sortKey="Basco, Leonardo K" sort="Basco, Leonardo K" uniqKey="Basco L" first="Leonardo K" last="Basco">Leonardo K. Basco</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno>
<idno type="RNSR">200817431S</idno>
<orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName>
<orgName type="acronym">URMITE</orgName>
<date type="start">2008-01-01</date>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.mediterranee-infection.com/</ref>
</desc>
<listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation>
<relation name="UM63" active="#struct-198056" type="direct"></relation>
<relation name="U1095" active="#struct-303623" type="direct"></relation>
<relation active="#struct-303759" type="direct"></relation>
<relation active="#struct-302108" type="indirect"></relation>
<relation name="UMR7278" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR_D198" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UM63" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1095" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303759" type="direct"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName>
<date type="start">1996-01-01</date>
<date type="end">2011-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-302108" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle name="UMR7278" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Tahar, Rachida" sort="Tahar, Rachida" uniqKey="Tahar R" first="Rachida" last="Tahar">Rachida Tahar</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-134260" status="OLD"> <idno type="IdRef">180320637</idno>
<idno type="ISNI">0000 0004 0508 7272 </idno>
<idno type="RNSR">201020824W</idno>
<orgName>Mère et enfant face aux infections tropicales</orgName>
<orgName type="acronym">MERIT - UMR_D 216</orgName>
<date type="start">2014-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté de pharmacie - Laboratoire de parasitologie4 avenue de l'Observatoire 75270 Paris cedex 6</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umr216.fr</ref>
</desc>
<listRelation> <relation active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 216" active="#struct-301664" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 216" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD"> <idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<date type="start">1971-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-01217921</idno>
<idno type="halId">hal-01217921</idno>
<idno type="halUri">https://hal-amu.archives-ouvertes.fr/hal-01217921</idno>
<idno type="url">https://hal-amu.archives-ouvertes.fr/hal-01217921</idno>
<idno type="doi">10.1186/s12936-015-0634-2</idno>
<date when="2015-03-14">2015-03-14</date>
<idno type="wicri:Area/Hal/Corpus">000163</idno>
<idno type="wicri:Area/Hal/Curation">000163</idno>
<idno type="wicri:Area/Hal/Checkpoint">000099</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000099</idno>
<idno type="wicri:doubleKey">1475-2875:2015:Foumane Ngane V:molecular:epidemiology:of</idno>
<idno type="wicri:Area/Main/Merge">000E53</idno>
<idno type="wicri:Area/Main/Curation">000E52</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular epidemiology of drug-resistant Plasmodium falciparum in Benguela province, Angola</title>
<author><name sortKey="Foumane Ngane, Vincent" sort="Foumane Ngane, Vincent" uniqKey="Foumane Ngane V" first="Vincent" last="Foumane Ngane">Vincent Foumane Ngane</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-81274" status="INCOMING"> <orgName>Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale</orgName>
<orgName type="acronym">OCEAC</orgName>
<desc> <address> <addrLine>BP 288, Yaoundé, Cameroon</addrLine>
<country key="CM"></country>
</address>
</desc>
<listRelation> <relation active="#struct-320801" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-320801" type="direct"><org type="institution" xml:id="struct-320801" status="INCOMING"> <orgName>Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Cameroun</country>
</affiliation>
</author>
<author><name sortKey="Djaman, Joseph Allico" sort="Djaman, Joseph Allico" uniqKey="Djaman J" first="Joseph Allico" last="Djaman">Joseph Allico Djaman</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-242267" status="VALID"> <orgName>Laboratoire de Pharmacodynamie Biochimique</orgName>
<desc> <address> <addrLine>01 BP V34 Abidjan 01</addrLine>
<country key="CI"></country>
</address>
</desc>
<listRelation> <relation active="#struct-218070" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-218070" type="direct"><org type="institution" xml:id="struct-218070" status="VALID"> <orgName>Université Félix Houphouët-Boigny</orgName>
<orgName type="acronym">UFHB</orgName>
<desc> <address> <addrLine> Abidjan</addrLine>
<country key="CI"></country>
</address>
<ref type="url">http://univ-fhb.edu.ci/fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Côte d'Ivoire</country>
</affiliation>
</author>
<author><name sortKey="Culeux, Cecile" sort="Culeux, Cecile" uniqKey="Culeux C" first="Cécile" last="Culeux">Cécile Culeux</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-134260" status="OLD"> <idno type="IdRef">180320637</idno>
<idno type="ISNI">0000 0004 0508 7272 </idno>
<idno type="RNSR">201020824W</idno>
<orgName>Mère et enfant face aux infections tropicales</orgName>
<orgName type="acronym">MERIT - UMR_D 216</orgName>
<date type="start">2014-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté de pharmacie - Laboratoire de parasitologie4 avenue de l'Observatoire 75270 Paris cedex 6</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umr216.fr</ref>
</desc>
<listRelation> <relation active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 216" active="#struct-301664" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 216" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD"> <idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<date type="start">1971-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Piette, Nathalie" sort="Piette, Nathalie" uniqKey="Piette N" first="Nathalie" last="Piette">Nathalie Piette</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-134260" status="OLD"> <idno type="IdRef">180320637</idno>
<idno type="ISNI">0000 0004 0508 7272 </idno>
<idno type="RNSR">201020824W</idno>
<orgName>Mère et enfant face aux infections tropicales</orgName>
<orgName type="acronym">MERIT - UMR_D 216</orgName>
<date type="start">2014-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté de pharmacie - Laboratoire de parasitologie4 avenue de l'Observatoire 75270 Paris cedex 6</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umr216.fr</ref>
</desc>
<listRelation> <relation active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 216" active="#struct-301664" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 216" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD"> <idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<date type="start">1971-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Carnevale, Pierre" sort="Carnevale, Pierre" uniqKey="Carnevale P" first="Pierre" last="Carnevale">Pierre Carnevale</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439701" status="INCOMING"> <orgName>Malaria Control Programme</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
<listRelation> <relation active="#struct-348140" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-348140" type="direct"><org type="institution" xml:id="struct-348140" status="INCOMING"> <orgName>Société Nationale de Métallurgie (Sonamet)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Angola</country>
</affiliation>
</author>
<author><name sortKey="Besnard, Patrick" sort="Besnard, Patrick" uniqKey="Besnard P" first="Patrick" last="Besnard">Patrick Besnard</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439701" status="INCOMING"> <orgName>Malaria Control Programme</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
<listRelation> <relation active="#struct-348140" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-348140" type="direct"><org type="institution" xml:id="struct-348140" status="INCOMING"> <orgName>Société Nationale de Métallurgie (Sonamet)</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Angola</country>
</affiliation>
</author>
<author><name sortKey="Fortes, Filomeno" sort="Fortes, Filomeno" uniqKey="Fortes F" first="Filomeno" last="Fortes">Filomeno Fortes</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439703" status="INCOMING"> <orgName>Plano National Contra Malaria</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
<listRelation> <relation active="#struct-439702" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-439702" type="direct"><org type="institution" xml:id="struct-439702" status="INCOMING"> <orgName>Ministry of Health</orgName>
<desc> <address> <country key="AO"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>Angola</country>
</affiliation>
</author>
<author><name sortKey="Basco, Leonardo K" sort="Basco, Leonardo K" uniqKey="Basco L" first="Leonardo K" last="Basco">Leonardo K. Basco</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199392" status="OLD"> <idno type="IdRef">123037891</idno>
<idno type="RNSR">200817431S</idno>
<orgName>Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes</orgName>
<orgName type="acronym">URMITE</orgName>
<date type="start">2008-01-01</date>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>27 Bd Jean Moulin 13385 MARSEILLE Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.mediterranee-infection.com/</ref>
</desc>
<listRelation> <relation name="UMR_D198" active="#struct-67872" type="direct"></relation>
<relation name="UM63" active="#struct-198056" type="direct"></relation>
<relation name="U1095" active="#struct-303623" type="direct"></relation>
<relation active="#struct-303759" type="direct"></relation>
<relation active="#struct-302108" type="indirect"></relation>
<relation name="UMR7278" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR_D198" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UM63" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1095" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303759" type="direct"><org type="regrouplaboratory" xml:id="struct-303759" status="OLD"> <orgName>IFR48</orgName>
<date type="start">1996-01-01</date>
<date type="end">2011-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
</desc>
<listRelation> <relation active="#struct-302108" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-302108" type="indirect"><org type="institution" xml:id="struct-302108" status="VALID"> <orgName>INSB</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle name="UMR7278" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Tahar, Rachida" sort="Tahar, Rachida" uniqKey="Tahar R" first="Rachida" last="Tahar">Rachida Tahar</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-134260" status="OLD"> <idno type="IdRef">180320637</idno>
<idno type="ISNI">0000 0004 0508 7272 </idno>
<idno type="RNSR">201020824W</idno>
<orgName>Mère et enfant face aux infections tropicales</orgName>
<orgName type="acronym">MERIT - UMR_D 216</orgName>
<date type="start">2014-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté de pharmacie - Laboratoire de parasitologie4 avenue de l'Observatoire 75270 Paris cedex 6</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umr216.fr</ref>
</desc>
<listRelation> <relation active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 216" active="#struct-301664" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 216" active="#struct-301664" type="direct"><org type="institution" xml:id="struct-301664" status="OLD"> <idno type="IdRef">026404788</idno>
<idno type="ISNI">0000 0001 2188 0914 </idno>
<orgName>Université Paris Descartes - Paris 5</orgName>
<orgName type="acronym">UPD5</orgName>
<date type="start">1971-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.parisdescartes.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1186/s12936-015-0634-2</idno>
<series><title level="j">Malaria Journal</title>
<idno type="ISSN">1475-2875</idno>
<imprint><date type="datePub">2015-03-14</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>AMODIAQUINE</term>
<term>Antifolate drugs</term>
<term>CHLOROQUINE</term>
<term>Drug resistance</term>
<term>GENE</term>
<term>LUANDA</term>
<term>LUMEFANTRINE</term>
<term>MALARIA</term>
<term>Molecular markers</term>
<term>NORTHERN ANGOLA</term>
<term>PFCRT</term>
<term>PFDHPS MUTATIONS</term>
<term>Plasmodium falciparum</term>
<term>SULFADOXINE-PYRIMETHAMINE</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"> <p>Background: The malaria situation has been worsening in Angola, partly due to armed conflict until the recent past and drug-resistant Plasmodium falciparum. Malaria transmission is heterogeneous within the country, and data on drug-resistant malaria in different parts of the country are incomplete. The aim of the present study was to evaluate resistance to 4-aminoquinolines and antifolate drugs in P. falciparum isolates collected in Benguela province, central Angola, using molecular markers. Methods: Fingerprick capillary blood was collected from asymptomatic children aged less than 15 years old during a household survey in and around Balombo town in 2010–2011. Samples were screened for P. falciparum by nested PCR. Molecular markers (P. falciparum dihydrofolate reductase [pfdhfr], P. falciparum dihydropteroate synthase [pfdhps], P. falciparum chloroquine resistance transporter [pfcrt], and P. falciparum multidrug-resistance gene 1 [pfmdr1]) were sequenced to determine the key codons associated with drug resistance. Results: A total of 60 blood samples were positive for P. falciparum. Most isolates with successful PCR amplification had mutant pfdhfr alleles, with either double mutant AICNI (69%) or triple mutant AIRNI (21%) haplotypes. A16V, S108T, and I164L substitutions were not found. Many of the isolates were carriers of either SGKAA (60%) or AGKAA (27%) pfdhps haplotype. K540E substitution was absent. There were only two pfcrt haplotypes: wild-type CVMNK (11%) and mutant CVIET (89%). Wild-type pfmdr1 NYSND haplotype was found in 19% of the isolates, whereas single mutant pfmdr1 YYSND and NFSND haplotypes occurred in 48% and 11%, respectively. Double mutant pfmdr1 haplotypes (YFSND and YYSNY) occurred rarely. Conclusions: The results suggest that the high prevalence of mutant pfcrt CVIET haplotype is in agreement with low clinical efficacy of chloroquine observed in earlier studies and that the double pfdhfr mutant AICNI and single pfdhps mutant SGKAA are currently the predominant haplotypes associated with antifolate resistance in Benguela province. The hallmark of clinical resistance observed in East Africa, i.e. triple pfdhfr mutant haplotype (AIRNI) and double pfdhps mutant haplotype (SGEAA), was absent. These molecular findings need to be further evaluated in parallel with clinical studies, in particular with the efficacy of intermittent preventive treatment using sulphadoxine-pyrimethamine in pregnant women and artesunate-amodiaquine for uncomplicated malaria.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E52 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000E52 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Main |étape= Curation |type= RBID |clé= Hal:hal-01217921 |texte= Molecular epidemiology of drug-resistant Plasmodium falciparum in Benguela province, Angola }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |