A platelet monoclonal antibody inhibition assay for detection of glycoprotein IIbIIIa-related platelet alloantibodies
Identifieur interne : 002B81 ( Istex/Curation ); précédent : 002B80; suivant : 002B82A platelet monoclonal antibody inhibition assay for detection of glycoprotein IIbIIIa-related platelet alloantibodies
Auteurs : Alexander P. Reiner [États-Unis] ; Gayle Teramura [États-Unis] ; Karen A. Nelson [États-Unis] ; Sherrill J. Slichter [États-Unis]Source :
- Journal of Immunological Methods [ 0022-1759 ] ; 1995.
English descriptors
- Teeft :
- Allele, Alloantibody, Antibody, Antibody concentration, Antibody inhibition assay, Antigen systems, Antiserum, Assay, Citric acid, Control plasma, Control sera, Detection system, Direct binding, Elisa, Epitope, Glycoprotein, Glycoprotein iiia, Gpiib, Gpiib assay, Gpiib inhibition assay, Gpiiia, Gpiiia assay, Homozygous donor, Homozygous platelets, Human albumin, Immunological, Immunological methods, Inhibition assay, Intact platelets, Kiefel, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoclonal inhibition assay, Neonatal alloimmune thrombocytopenia, Normal serum, Phenotype, Phenotype determination, Platelet, Platelet antigen typing, Platelet donors, Platelet glycoprotein, Platelet panel, Puget sound blood center, Random donors, Random individuals, Room temperature, Substrate solution.
Abstract
Abstract: Post-transfusion purpura (PTP) and neonatal alloimmune thrombocytopenia (NAT) result from formation of alloantibodies to platelet membrane glycoprotein-associated antigens. The detection and identification of platelet-specific alloantibodies in patient sera is often complicated by the presence of co-existing HLA antibodies and/or more than one platelet specificity in the same serum. We describe a solid phase assay that specifically detects antibodies to platelet membrane associated alloantigens by measuring the ability of patient antisera to inhibit the binding of glycoprotein GPIIb or GPIIIa monoclonal antibodies to intact platelets. When tested in the GPIIIa assay against a panel of random platelet donors, the reactivities of two known PLA1 antisera that also contained different HLA antibodies were highly correlated (r = 0.99) and allowed PLA phenotyping of the population. A standard direct binding platelet ELISA, on the other hand, was unable to accurately PLA phenotype the same population. The reactivities of two known Baka antisera (one containing additional anti-PLA2 and the other anti-Brb specificities) were highly correlated (r = 0.95) in the GPIIb assay, and Bak phenotype determination was similarly accomplished for a random platelet panel. Furthermore, a comparison of platelet phenotype results (using the monoclonal inhibition assay) and genotype results (using DNA analysis) for the PLA and Bak systems showed a concordance of 98% for 146 alleles tested. In conclusion, the platelet monoclonal antibody inhibition assay: (1) allows determination of platelet-specific alloantibodies in the presence of contaminating HLA antibodies and/or in sera containing multiple platelet alloantibodies; (2) allows accurate platelet phenotyping for the GPIIIa-associated PLA and GPIIb-associated Bak antigen systems; and (3) may be applicable to the detection of other known or even novel platelet glycoprotein-associated antigens.
Url:
DOI: 10.1016/0022-1759(95)00083-M
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Reiner</name><affiliation wicri:level="1"><mods:affiliation>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA</wicri:regionArea></affiliation></author><author><name sortKey="Teramura, Gayle" sort="Teramura, Gayle" uniqKey="Teramura G" first="Gayle" last="Teramura">Gayle Teramura</name><affiliation wicri:level="1"><mods:affiliation>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA</wicri:regionArea></affiliation></author><author><name sortKey="Nelson, Karen A" sort="Nelson, Karen A" uniqKey="Nelson K" first="Karen A." last="Nelson">Karen A. 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Reiner</name><affiliation wicri:level="1"><mods:affiliation>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA</wicri:regionArea></affiliation></author><author><name sortKey="Teramura, Gayle" sort="Teramura, Gayle" uniqKey="Teramura G" first="Gayle" last="Teramura">Gayle Teramura</name><affiliation wicri:level="1"><mods:affiliation>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA</wicri:regionArea></affiliation></author><author><name sortKey="Nelson, Karen A" sort="Nelson, Karen A" uniqKey="Nelson K" first="Karen A." last="Nelson">Karen A. Nelson</name><affiliation wicri:level="1"><mods:affiliation>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA</wicri:regionArea></affiliation></author><author><name sortKey="Slichter, Sherrill J" sort="Slichter, Sherrill J" uniqKey="Slichter S" first="Sherrill J." last="Slichter">Sherrill J. Slichter</name><affiliation wicri:level="1"><mods:affiliation>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Puget Sound Blood Center, and Department of Medicine, University of Washington School of Medicine, Seattle, WA</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">184</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="153">153</biblScope><biblScope unit="page" to="162">162</biblScope></imprint><idno type="ISSN">0022-1759</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Allele</term><term>Alloantibody</term><term>Antibody</term><term>Antibody concentration</term><term>Antibody inhibition assay</term><term>Antigen systems</term><term>Antiserum</term><term>Assay</term><term>Citric acid</term><term>Control plasma</term><term>Control sera</term><term>Detection system</term><term>Direct binding</term><term>Elisa</term><term>Epitope</term><term>Glycoprotein</term><term>Glycoprotein iiia</term><term>Gpiib</term><term>Gpiib assay</term><term>Gpiib inhibition assay</term><term>Gpiiia</term><term>Gpiiia assay</term><term>Homozygous donor</term><term>Homozygous platelets</term><term>Human albumin</term><term>Immunological</term><term>Immunological methods</term><term>Inhibition assay</term><term>Intact platelets</term><term>Kiefel</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Monoclonal inhibition assay</term><term>Neonatal alloimmune thrombocytopenia</term><term>Normal serum</term><term>Phenotype</term><term>Phenotype determination</term><term>Platelet</term><term>Platelet antigen typing</term><term>Platelet donors</term><term>Platelet glycoprotein</term><term>Platelet panel</term><term>Puget sound blood center</term><term>Random donors</term><term>Random individuals</term><term>Room temperature</term><term>Substrate solution</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Post-transfusion purpura (PTP) and neonatal alloimmune thrombocytopenia (NAT) result from formation of alloantibodies to platelet membrane glycoprotein-associated antigens. The detection and identification of platelet-specific alloantibodies in patient sera is often complicated by the presence of co-existing HLA antibodies and/or more than one platelet specificity in the same serum. We describe a solid phase assay that specifically detects antibodies to platelet membrane associated alloantigens by measuring the ability of patient antisera to inhibit the binding of glycoprotein GPIIb or GPIIIa monoclonal antibodies to intact platelets. When tested in the GPIIIa assay against a panel of random platelet donors, the reactivities of two known PLA1 antisera that also contained different HLA antibodies were highly correlated (r = 0.99) and allowed PLA phenotyping of the population. A standard direct binding platelet ELISA, on the other hand, was unable to accurately PLA phenotype the same population. The reactivities of two known Baka antisera (one containing additional anti-PLA2 and the other anti-Brb specificities) were highly correlated (r = 0.95) in the GPIIb assay, and Bak phenotype determination was similarly accomplished for a random platelet panel. Furthermore, a comparison of platelet phenotype results (using the monoclonal inhibition assay) and genotype results (using DNA analysis) for the PLA and Bak systems showed a concordance of 98% for 146 alleles tested. In conclusion, the platelet monoclonal antibody inhibition assay: (1) allows determination of platelet-specific alloantibodies in the presence of contaminating HLA antibodies and/or in sera containing multiple platelet alloantibodies; (2) allows accurate platelet phenotyping for the GPIIIa-associated PLA and GPIIb-associated Bak antigen systems; and (3) may be applicable to the detection of other known or even novel platelet glycoprotein-associated antigens.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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