Ceramide metabolism regulates autophagy and apoptotic cell death induced by melatonin in liver cancer cells
Identifieur interne : 001288 ( Istex/Curation ); précédent : 001287; suivant : 001289Ceramide metabolism regulates autophagy and apoptotic cell death induced by melatonin in liver cancer cells
Auteurs : Raquel Ordo Ez [Espagne] ; Anna Fernández [Espagne] ; Néstor Prieto-Domínguez [Espagne] ; Laura Martínez [Espagne] ; Carmen García-Ruiz [Espagne, États-Unis] ; José C. Fernández-Checa [Espagne, États-Unis] ; José L. Mauriz [Espagne] ; Javier González-Gallego [Espagne]Source :
- Journal of Pineal Research [ 0742-3098 ] ; 2015-09.
Abstract
Autophagy is a process that maintains homeostasis during stress, although it also contributes to cell death under specific contexts. Ceramides have emerged as important effectors in the regulation of autophagy, mediating the crosstalk with apoptosis. Melatonin induces apoptosis of cancer cells; however, its role in autophagy and ceramide metabolism has yet to be clearly elucidated. This study was aimed to evaluate the effect of melatonin administration on autophagy and ceramide metabolism and its possible link with melatonin‐induced apoptotic cell death in hepatocarcinoma (HCC) cells. Melatonin (2 mm) transiently induced autophagy in HepG2 cells through JNK phosphorylation, characterized by increased Beclin‐1 expression, p62 degradation, and LC3II and LAMP‐2 colocalization, which translated in decreased cell viability. Moreover, ATG5 silencing sensitized HepG2 cells to melatonin‐induced apoptosis, suggesting a dual role of autophagy in cell death. Melatonin enhanced ceramide levels through both de novo synthesis and acid sphingomyelinase (ASMase) stimulation. Serine palmitoyltransferase (SPT) inhibition with myriocin prevented melatonin‐induced autophagy and ASMase inhibition with imipramine‐impaired autophagy flux. However, ASMase inhibition partially protected HepG2 cells against melatonin, while SPT inhibition significantly enhanced cell death. Findings suggest a crosstalk between SPT‐mediated ceramide generation and autophagy in protecting against melatonin, while specific ASMase‐induced ceramide production participates in melatonin‐mediated cell death. Thus, dual blocking of SPT and autophagy emerges as a potential strategy to potentiate the apoptotic effects of melatonin in liver cancer cells.
Url:
DOI: 10.1111/jpi.12249
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001288
Links to Exploration step
ISTEX:2E7E9C7ED7E1070AE0836E5AFBE9D987A830CB26Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ceramide metabolism regulates autophagy and apoptotic cell death induced by melatonin in liver cancer cells</title><author><name sortKey="Ordo Ez, Raquel" sort="Ordo Ez, Raquel" uniqKey="Ordo Ez R" first="Raquel" last="Ordo Ez">Raquel Ordo Ez</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Fernandez, Anna" sort="Fernandez, Anna" uniqKey="Fernandez A" first="Anna" last="Fernández">Anna Fernández</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Prieto Ominguez, Nestor" sort="Prieto Ominguez, Nestor" uniqKey="Prieto Ominguez N" first="Néstor" last="Prieto-Domínguez">Néstor Prieto-Domínguez</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Martinez, Laura" sort="Martinez, Laura" uniqKey="Martinez L" first="Laura" last="Martínez">Laura Martínez</name><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona</wicri:regionArea></affiliation></author><author><name sortKey="Garcia Uiz, Carmen" sort="Garcia Uiz, Carmen" uniqKey="Garcia Uiz C" first="Carmen" last="García-Ruiz">Carmen García-Ruiz</name><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles</wicri:regionArea></affiliation></author><author><name sortKey="Fernandez Heca, Jose C" sort="Fernandez Heca, Jose C" uniqKey="Fernandez Heca J" first="José C." last="Fernández-Checa">José C. Fernández-Checa</name><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles</wicri:regionArea></affiliation></author><author><name sortKey="Mauriz, Jose L" sort="Mauriz, Jose L" uniqKey="Mauriz J" first="José L." last="Mauriz">José L. Mauriz</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Gonzalez Allego, Javier" sort="Gonzalez Allego, Javier" uniqKey="Gonzalez Allego J" first="Javier" last="González-Gallego">Javier González-Gallego</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation><mods:affiliation>Address reprint requests to Javier González‐Gallego, PhD MD, Institute of Biomedicine, University of León, 24071, León, Spain.E‐mail:</mods:affiliation><wicri:noCountry code="subField">Spain.E‐mail:</wicri:noCountry></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: jgonga@unileon.es</mods:affiliation><country wicri:rule="url">Espagne</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2E7E9C7ED7E1070AE0836E5AFBE9D987A830CB26</idno><date when="2015" year="2015">2015</date><idno type="doi">10.1111/jpi.12249</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-X2700BR0-B/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001288</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001288</idno><idno type="wicri:Area/Istex/Curation">001288</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Ceramide metabolism regulates autophagy and apoptotic cell death induced by melatonin in liver cancer cells</title><author><name sortKey="Ordo Ez, Raquel" sort="Ordo Ez, Raquel" uniqKey="Ordo Ez R" first="Raquel" last="Ordo Ez">Raquel Ordo Ez</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Fernandez, Anna" sort="Fernandez, Anna" uniqKey="Fernandez A" first="Anna" last="Fernández">Anna Fernández</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Prieto Ominguez, Nestor" sort="Prieto Ominguez, Nestor" uniqKey="Prieto Ominguez N" first="Néstor" last="Prieto-Domínguez">Néstor Prieto-Domínguez</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Martinez, Laura" sort="Martinez, Laura" uniqKey="Martinez L" first="Laura" last="Martínez">Laura Martínez</name><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona</wicri:regionArea></affiliation></author><author><name sortKey="Garcia Uiz, Carmen" sort="Garcia Uiz, Carmen" uniqKey="Garcia Uiz C" first="Carmen" last="García-Ruiz">Carmen García-Ruiz</name><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles</wicri:regionArea></affiliation></author><author><name sortKey="Fernandez Heca, Jose C" sort="Fernandez Heca, Jose C" uniqKey="Fernandez Heca J" first="José C." last="Fernández-Checa">José C. Fernández-Checa</name><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Cell Death and Proliferation, Institute of Biomedical Research of Barcelona (IIBB), Consejo Superior Investigaciones Cientificas (CSIC) and Liver Unit‐Hospital Clinic, Barcelona</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Keck School of Medicine, USC, University of Southern California Research Center for Alcohol Liver and Pancreatic Diseases and Cirrhosis, CA, Los Angeles</wicri:regionArea></affiliation></author><author><name sortKey="Mauriz, Jose L" sort="Mauriz, Jose L" uniqKey="Mauriz J" first="José L." last="Mauriz">José L. Mauriz</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation></author><author><name sortKey="Gonzalez Allego, Javier" sort="Gonzalez Allego, Javier" uniqKey="Gonzalez Allego J" first="Javier" last="González-Gallego">Javier González-Gallego</name><affiliation wicri:level="1"><mods:affiliation>Institute of Biomedicine (IBIOMED), University of León, León, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Institute of Biomedicine (IBIOMED), University of León, León</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd)</wicri:regionArea></affiliation><affiliation><mods:affiliation>Address reprint requests to Javier González‐Gallego, PhD MD, Institute of Biomedicine, University of León, 24071, León, Spain.E‐mail:</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: jgonga@unileon.es</mods:affiliation><country wicri:rule="url">Espagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Pineal Research</title><title level="j" type="alt">JOURNAL OF PINEAL RESEARCH</title><idno type="ISSN">0742-3098</idno><idno type="eISSN">1600-079X</idno><imprint><biblScope unit="vol">59</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="178">178</biblScope><biblScope unit="page" to="189">189</biblScope><biblScope unit="page-count">12</biblScope><date type="published" when="2015-09">2015-09</date></imprint><idno type="ISSN">0742-3098</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0742-3098</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Autophagy is a process that maintains homeostasis during stress, although it also contributes to cell death under specific contexts. Ceramides have emerged as important effectors in the regulation of autophagy, mediating the crosstalk with apoptosis. Melatonin induces apoptosis of cancer cells; however, its role in autophagy and ceramide metabolism has yet to be clearly elucidated. This study was aimed to evaluate the effect of melatonin administration on autophagy and ceramide metabolism and its possible link with melatonin‐induced apoptotic cell death in hepatocarcinoma (HCC) cells. Melatonin (2 mm) transiently induced autophagy in HepG2 cells through JNK phosphorylation, characterized by increased Beclin‐1 expression, p62 degradation, and LC3II and LAMP‐2 colocalization, which translated in decreased cell viability. Moreover, ATG5 silencing sensitized HepG2 cells to melatonin‐induced apoptosis, suggesting a dual role of autophagy in cell death. Melatonin enhanced ceramide levels through both de novo synthesis and acid sphingomyelinase (ASMase) stimulation. Serine palmitoyltransferase (SPT) inhibition with myriocin prevented melatonin‐induced autophagy and ASMase inhibition with imipramine‐impaired autophagy flux. However, ASMase inhibition partially protected HepG2 cells against melatonin, while SPT inhibition significantly enhanced cell death. Findings suggest a crosstalk between SPT‐mediated ceramide generation and autophagy in protecting against melatonin, while specific ASMase‐induced ceramide production participates in melatonin‐mediated cell death. Thus, dual blocking of SPT and autophagy emerges as a potential strategy to potentiate the apoptotic effects of melatonin in liver cancer cells.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001288 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001288 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:2E7E9C7ED7E1070AE0836E5AFBE9D987A830CB26
|texte= Ceramide metabolism regulates autophagy and apoptotic cell death induced by melatonin in liver cancer cells
}}
| This area was generated with Dilib version V0.6.33. |  |