Anti‐Granulocyte Antibody Screening with Extracted Granulocyte Antigens by a Micro‐Mixed Passive Hemagglutination Method
Identifieur interne : 000623 ( Istex/Curation ); précédent : 000622; suivant : 000624Anti‐Granulocyte Antibody Screening with Extracted Granulocyte Antigens by a Micro‐Mixed Passive Hemagglutination Method
Auteurs : Nobuo Araki [Japon] ; Yoshisuke Nose [Japon] ; Masatoshi Kohsaki [Japon] ; Hisashi Mito [Japon] ; Kazuhiko Ito [Japon]Source :
- Vox Sanguinis [ 0042-9007 ] ; 1999-08.
Abstract
Background and Objectives: Serologic tests for granulocyte antibodies, i.e., the granulocyte agglutination test and the granulocyte immunofluorescence test, require panels of typed granulocytes that cannot be preserved for more than a few hours. We have developed a new method in which granulocyte antigens, extrcated into saline containing 3% sucrose, are coated onto U‐type Terasaki plates. With this new method, we evaluated the micro‐mixed passive hemagglutination test (EG‐MPHA) for screening for granulocyte antibodies. Materials and Methods: We tested the ability of the EG‐MPHA to detect granulocyte antigens using 5 human antibodies specific for NA1, NA2, NB1, 5b, and Sara, and 8 different monoclonal antibodies for NA1, CD11a, CD11b, CD13, CD16, CD18 and HLA class I. Sera from 94 alloimmunized patients were screened by the chloroquine‐treated EG‐MPHA method. Results: Na1, NA2, NB1, 5b, Sara, CD11a, CD11b, CD13, CD16, CD18 and HLA class I antigens were present in the extracted granulocyte antigen preparation. CD11b and HLA class I antigens were removed when the extracted granulocyte antigens were treated with chloroquine. Granulocyte antibody screening of sera from alloimmunized patients showed that approximately 30% of the anti‐HLA‐positive for granulocyte antibody by the chloroquine‐treated EG‐MPHA. The extracted granulocyte antigen panels could be stored frozen for at least 1 year at −80°C. Conclusion: This new method is preferable for screening for granulocyte antibodies. In addition, it has the advantage of requiring only 5 μl of serum for each test.
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DOI: 10.1046/j.1423-0410.1999.7710044.x
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first="Yoshisuke" last="Nose">Yoshisuke Nose</name><affiliation wicri:level="1"><mods:affiliation>Hyogo Red Cross Blood Center, Hyogo, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Hyogo Red Cross Blood Center, Hyogo</wicri:regionArea></affiliation></author><author><name sortKey="Kohsaki, Masatoshi" sort="Kohsaki, Masatoshi" uniqKey="Kohsaki M" first="Masatoshi" last="Kohsaki">Masatoshi Kohsaki</name><affiliation wicri:level="1"><mods:affiliation>Hyogo Red Cross Blood Center, Hyogo, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Hyogo Red Cross Blood Center, Hyogo</wicri:regionArea></affiliation></author><author><name sortKey="Mito, Hisashi" sort="Mito, Hisashi" uniqKey="Mito H" first="Hisashi" last="Mito">Hisashi Mito</name><affiliation wicri:level="1"><mods:affiliation>Hyogo Red Cross Blood Center, Hyogo, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Hyogo Red Cross Blood Center, 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We have developed a new method in which granulocyte antigens, extrcated into saline containing 3% sucrose, are coated onto U‐type Terasaki plates. With this new method, we evaluated the micro‐mixed passive hemagglutination test (EG‐MPHA) for screening for granulocyte antibodies. Materials and Methods: We tested the ability of the EG‐MPHA to detect granulocyte antigens using 5 human antibodies specific for NA1, NA2, NB1, 5b, and Sara, and 8 different monoclonal antibodies for NA1, CD11a, CD11b, CD13, CD16, CD18 and HLA class I. Sera from 94 alloimmunized patients were screened by the chloroquine‐treated EG‐MPHA method. Results: Na1, NA2, NB1, 5b, Sara, CD11a, CD11b, CD13, CD16, CD18 and HLA class I antigens were present in the extracted granulocyte antigen preparation. CD11b and HLA class I antigens were removed when the extracted granulocyte antigens were treated with chloroquine. Granulocyte antibody screening of sera from alloimmunized patients showed that approximately 30% of the anti‐HLA‐positive for granulocyte antibody by the chloroquine‐treated EG‐MPHA. The extracted granulocyte antigen panels could be stored frozen for at least 1 year at −80°C. Conclusion: This new method is preferable for screening for granulocyte antibodies. In addition, it has the advantage of requiring only 5 μl of serum for each test.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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