Photochemical Transfection: A Technology for Efficient Light-Directed Gene Delivery
Identifieur interne : 002917 ( Istex/Corpus ); précédent : 002916; suivant : 002918Photochemical Transfection: A Technology for Efficient Light-Directed Gene Delivery
Auteurs : Anders H Gset ; Lina Prasmickaite ; Marit Hellum ; Birgit Enges Ter ; Vibeke M. Olsen ; Torunn E. Tjelle ; Carl J. Wheeler ; Kristian BergSource :
- Somatic Cell and Molecular Genetics [ 0740-7750 ] ; 2002-11-01.
Abstract
Abstract: Most synthetic gene delivery vectors are taken up in the cell by endocytosis, and inefficient escape of the transgene from endocytic vesicles often is a major barrier for gene transfer by such vectors. To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vivo, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a ) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in in vivo gene therapy is discussed.
Url:
DOI: 10.1023/A:1022979806314
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Olsen</name><affiliation><mods:affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</mods:affiliation></affiliation></author><author><name sortKey="Tjelle, Torunn E" sort="Tjelle, Torunn E" uniqKey="Tjelle T" first="Torunn E." last="Tjelle">Torunn E. Tjelle</name><affiliation><mods:affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</mods:affiliation></affiliation></author><author><name sortKey="Wheeler, Carl J" sort="Wheeler, Carl J" uniqKey="Wheeler C" first="Carl J." last="Wheeler">Carl J. Wheeler</name><affiliation><mods:affiliation>Vical Inc., 9373 Towne Centre Drive, 92121, San Diego, California</mods:affiliation></affiliation></author><author><name sortKey="Berg, Kristian" sort="Berg, Kristian" uniqKey="Berg K" first="Kristian" last="Berg">Kristian Berg</name><affiliation><mods:affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Somatic Cell and Molecular Genetics</title><title level="j" type="abbrev">Somat Cell Mol Genet</title><idno type="ISSN">0740-7750</idno><idno type="eISSN">1572-9931</idno><imprint><publisher>Kluwer Academic Publishers-Plenum Publishers</publisher><pubPlace>New York</pubPlace><date type="published" when="2002-11-01">2002-11-01</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">1-6</biblScope><biblScope unit="page" from="97">97</biblScope><biblScope unit="page" to="113">113</biblScope></imprint><idno type="ISSN">0740-7750</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0740-7750</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Most synthetic gene delivery vectors are taken up in the cell by endocytosis, and inefficient escape of the transgene from endocytic vesicles often is a major barrier for gene transfer by such vectors. To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vivo, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a ) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in in vivo gene therapy is discussed.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Anders Høgset</name><affiliations><json:string>PCI Biotech AS, Hoffsvn. 48, N-0377, Oslo, Norway</json:string><json:string>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</json:string></affiliations></json:item><json:item><name>Lina Prasmickaite</name><affiliations><json:string>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</json:string></affiliations></json:item><json:item><name>Marit Hellum</name><affiliations><json:string>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</json:string></affiliations></json:item><json:item><name>Birgit Ø. Engesæter</name><affiliations><json:string>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</json:string><json:string>Department of Tumor Biology, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</json:string></affiliations></json:item><json:item><name>Vibeke M. Olsen</name><affiliations><json:string>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</json:string></affiliations></json:item><json:item><name>Torunn E. Tjelle</name><affiliations><json:string>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</json:string></affiliations></json:item><json:item><name>Carl J. 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PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vivo, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a ) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. 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xml:lang="en"><p>Abstract: Most synthetic gene delivery vectors are taken up in the cell by endocytosis, and inefficient escape of the transgene from endocytic vesicles often is a major barrier for gene transfer by such vectors. To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vivo, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a ) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in in vivo gene therapy is discussed.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Human Genetics</term></item><item><term>Biochemistry, general</term></item><item><term>Plant Sciences</term></item><item><term>Animal Anatomy / Morphology / Histology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2002-11-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-3Q3F9994-8/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" 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DisplayOrder="Western"><GivenName>Anders</GivenName><FamilyName>Høgset</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Lina</GivenName><FamilyName>Prasmickaite</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Marit</GivenName><FamilyName>Hellum</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>Birgit</GivenName><GivenName>Ø.</GivenName><FamilyName>Engesæter</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Vibeke</GivenName><GivenName>M.</GivenName><FamilyName>Olsen</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Torunn</GivenName><GivenName>E.</GivenName><FamilyName>Tjelle</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Carl</GivenName><GivenName>J.</GivenName><FamilyName>Wheeler</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Kristian</GivenName><FamilyName>Berg</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgName>PCI Biotech AS</OrgName><OrgAddress><Street>Hoffsvn. 48</Street><Postcode>N-0377</Postcode><City>Oslo</City><Country>Norway</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Biophysics</OrgDivision><OrgName>The Norwegian Radium Hospital, Montebello</OrgName><OrgAddress><Postcode>N-0310</Postcode><City>Oslo</City><Country>Norway</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Department of Tumor Biology</OrgDivision><OrgName>The Norwegian Radium Hospital, Montebello</OrgName><OrgAddress><Postcode>N-0310</Postcode><City>Oslo</City><Country>Norway</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgName>Vical Inc.</OrgName><OrgAddress><Street>9373 Towne Centre Drive</Street><City>San Diego</City><State>California</State><Postcode>92121</Postcode></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Most synthetic gene delivery vectors are taken up in the cell by endocytosis, and inefficient escape of the transgene from endocytic vesicles often is a major barrier for gene transfer by such vectors. To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method <Emphasis Type="Italic">in vivo</Emphasis>, which potentially can be developed into a site-specific method for gene delivery in <Emphasis Type="Italic">in vivo</Emphasis> gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS<Subscript>2<Emphasis Type="Italic">a</Emphasis></Subscript>) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in <Emphasis Type="Italic">in vivo</Emphasis> gene therapy is discussed.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Photochemical Transfection: A Technology for Efficient Light-Directed Gene Delivery</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Photochemical Transfection: A Technology for Efficient Light-Directed Gene Delivery</title></titleInfo><name type="personal"><namePart type="given">Anders</namePart><namePart type="family">Høgset</namePart><affiliation>PCI Biotech AS, Hoffsvn. 48, N-0377, Oslo, Norway</affiliation><affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lina</namePart><namePart type="family">Prasmickaite</namePart><affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marit</namePart><namePart type="family">Hellum</namePart><affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Birgit</namePart><namePart type="given">Ø.</namePart><namePart type="family">Engesæter</namePart><affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><affiliation>Department of Tumor Biology, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vibeke</namePart><namePart type="given">M.</namePart><namePart type="family">Olsen</namePart><affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Torunn</namePart><namePart type="given">E.</namePart><namePart type="family">Tjelle</namePart><affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Carl</namePart><namePart type="given">J.</namePart><namePart type="family">Wheeler</namePart><affiliation>Vical Inc., 9373 Towne Centre Drive, 92121, San Diego, California</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kristian</namePart><namePart type="family">Berg</namePart><affiliation>Department of Biophysics, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers-Plenum Publishers</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">2002-11-01</dateIssued><copyrightDate encoding="w3cdtf">2002</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Most synthetic gene delivery vectors are taken up in the cell by endocytosis, and inefficient escape of the transgene from endocytic vesicles often is a major barrier for gene transfer by such vectors. To improve endosomal release we have developed a new technology, named photochemical internalization (PCI). PCI is based on photochemical reactions initiated by photosensitizing compounds localized in endocytic vesicles, inducing rupture of these vesicles upon light exposure. PCI constitutes an efficient light-inducible gene transfer method in vivo, which potentially can be developed into a site-specific method for gene delivery in in vivo gene therapy. In this paper the principle behind the PCI technology and the effect of PCI on transfection with different synthetic gene delivery vectors are reviewed. PCI treatment by the photosensitizer aluminum phthalocyanine (AlPcS2a ) strongly improves transfection mediated by cationic polymers (e.g., poly-L-lysine and polyethylenimine), while the effect on transfection with cationic lipids is more variable. The timing of the light treatment relative to the transfection period was also important, indicating that release of the DNA from early endosomes is important for the outcome of PCI-induced transfection. The possibilities of using PCI as a technology for efficient, site-specific gene delivery in in vivo gene therapy is discussed.</abstract><relatedItem type="host"><titleInfo><title>Somatic Cell and Molecular Genetics</title></titleInfo><titleInfo type="abbreviated"><title>Somat Cell Mol Genet</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2002-11-01</dateIssued><copyrightDate encoding="w3cdtf">2002</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Human Genetics</topic><topic>Biochemistry, general</topic><topic>Plant Sciences</topic><topic>Animal Anatomy / Morphology / Histology</topic></subject><identifier type="ISSN">0740-7750</identifier><identifier type="eISSN">1572-9931</identifier><identifier type="JournalID">11188</identifier><identifier type="IssueArticleCount">10</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>2002</date><detail type="volume"><number>27</number><caption>vol.</caption></detail><detail type="issue"><number>1-6</number><caption>no.</caption></detail><extent unit="pages"><start>97</start><end>113</end></extent></part><recordInfo><recordOrigin>Plenum Publishing Corporation, 2002</recordOrigin></recordInfo></relatedItem><identifier type="istex">33FF7AB48CC46104A201ACF94D4E7C0C6D2CF7CA</identifier><identifier type="ark">ark:/67375/VQC-3Q3F9994-8</identifier><identifier type="DOI">10.1023/A:1022979806314</identifier><identifier type="ArticleID">457014</identifier><identifier type="ArticleID">Art9</identifier><accessCondition type="use and reproduction" contentType="copyright">Plenum Publishing Corporation, 2002</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Plenum Publishing Corporation, 2002</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-3Q3F9994-8/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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