Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes
Identifieur interne : 002734 ( Istex/Corpus ); précédent : 002733; suivant : 002735Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes
Auteurs : M. Del Zompo ; M. P. Piccardi ; S. Ruiu ; G. U. Corsini ; A. VaccariSource :
- Brain Research [ 0006-8993 ] ; 1992.
English descriptors
- KwdEn :
- Teeft :
- Adenosine triphosphate, Appropriate assay conditions, Assay, Binding site, Binding sites, Brain area, Brain research, Chromaffin granules, Chronic parkinsonism, Dopamine, Dopaminergic neurons, High affinity, Ligand program, Monoamine, Monoamine oxidase, Mouse brain, Mouse brains, Mouse striatal membranes, Mouse striatum, Mptp, Pharmacol, Potent displacers, Present assay conditions, Right striatum, Specific binding, Striatal, Striatum, Subcellular distribution, Synaptic vesicles, Synaptosomal fraction, Toxic metabolite, Vesicle, Vesicular monoamine transporter.
Abstract
Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.
Url:
DOI: 10.1016/0006-8993(92)90677-2
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</div>
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<ce:cross-ref refid="cor1"><ce:sup loc="post">*</ce:sup>
</ce:cross-ref>
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</ce:author>
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<ce:author><ce:given-name>G.U.</ce:given-name>
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</ce:author>
<ce:affiliation id="aff1"><ce:label>a</ce:label>
<ce:textfn>Department of Neuroscience, University of Cagliari, Cagliari, Italy</ce:textfn>
</ce:affiliation>
<ce:affiliation id="aff2"><ce:label>b</ce:label>
<ce:textfn>Institute of Pharmacology, Medical School, Pisa, Italy</ce:textfn>
</ce:affiliation>
<ce:correspondence id="cor1"><ce:label>*</ce:label>
<ce:text><ce:italic>Correspondence:</ce:italic>
M. Del Zompo, Department of Neuroscience, ‘B.B.Brodie’, University ofCagliari, Via Porcell, 4, I-09124 Cagliari, Italy.</ce:text>
</ce:correspondence>
</ce:author-group>
<ce:date-accepted day="29" month="10" year="1991"></ce:date-accepted>
<ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">[<ce:sup loc="pre">3</ce:sup>
H] <ce:italic>N</ce:italic>
-methyl-4-phenylpyridinium ion (MPP<ce:sup loc="post">+</ce:sup>
) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (<ce:italic>B</ce:italic>
<ce:inf loc="post">max</ce:inf>
= 168 ± 15fmol/mg protein, <ce:italic>K</ce:italic>
<ce:inf loc="post">D</ce:inf>
= 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [<ce:sup loc="pre">3</ce:sup>
H]MPP<ce:sup loc="post">+</ce:sup>
sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [<ce:sup loc="pre">3</ce:sup>
H]MPP<ce:sup loc="post">+</ce:sup>
, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg<ce:sup loc="post">2+</ce:sup>
-ions did not affect [<ce:sup loc="pre">3</ce:sup>
H]MPP<ce:sup loc="post">+</ce:sup>
binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
<ce:keywords class="keyword" xml:lang="en"><ce:section-title>Keywords</ce:section-title>
<ce:keyword><ce:text><ce:italic>N</ce:italic>
-Methyl-4-penylpyridinium ion</ce:text>
</ce:keyword>
<ce:keyword><ce:text>Binding site</ce:text>
</ce:keyword>
<ce:keyword><ce:text>Vesicle</ce:text>
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<ce:keyword><ce:text>Striatum</ce:text>
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<ce:keyword><ce:text>Mouse</ce:text>
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<mods version="3.6"><titleInfo lang="en"><title>Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes</title>
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<titleInfo type="alternative" lang="en" contentType="CDATA"><title>Characterization of a putatively vesicular binding site for [</title>
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<name type="personal"><namePart type="given">M.</namePart>
<namePart type="family">Del Zompo</namePart>
<affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation>
<description>Correspondence: M. Del Zompo, Department of Neuroscience, ‘B.B.Brodie’, University ofCagliari, Via Porcell, 4, I-09124 Cagliari, Italy.</description>
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<name type="personal"><namePart type="given">M.P.</namePart>
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<affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation>
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<name type="personal"><namePart type="given">G.U.</namePart>
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<affiliation>Institute of Pharmacology, Medical School, Pisa, Italy</affiliation>
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<namePart type="family">Vaccari</namePart>
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<abstract lang="en">Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</abstract>
<subject lang="en"><genre>Keywords</genre>
<topic>N-Methyl-4-penylpyridinium ion</topic>
<topic>Binding site</topic>
<topic>Vesicle</topic>
<topic>Striatum</topic>
<topic>Mouse</topic>
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