Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes
Identifieur interne : 002734 ( Istex/Corpus ); précédent : 002733; suivant : 002735Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes
Auteurs : M. Del Zompo ; M. P. Piccardi ; S. Ruiu ; G. U. Corsini ; A. VaccariSource :
- Brain Research [ 0006-8993 ] ; 1992.
English descriptors
- KwdEn :
- Teeft :
- Adenosine triphosphate, Appropriate assay conditions, Assay, Binding site, Binding sites, Brain area, Brain research, Chromaffin granules, Chronic parkinsonism, Dopamine, Dopaminergic neurons, High affinity, Ligand program, Monoamine, Monoamine oxidase, Mouse brain, Mouse brains, Mouse striatal membranes, Mouse striatum, Mptp, Pharmacol, Potent displacers, Present assay conditions, Right striatum, Specific binding, Striatal, Striatum, Subcellular distribution, Synaptic vesicles, Synaptosomal fraction, Toxic metabolite, Vesicle, Vesicular monoamine transporter.
Abstract
Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.
Url:
DOI: 10.1016/0006-8993(92)90677-2
Links to Exploration step
ISTEX:416F953064520C81F6066BE7F86493579150BEAALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes</title><author><name sortKey="Del Zompo, M" sort="Del Zompo, M" uniqKey="Del Zompo M" first="M." last="Del Zompo">M. Del Zompo</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Piccardi, M P" sort="Piccardi, M P" uniqKey="Piccardi M" first="M. P." last="Piccardi">M. P. Piccardi</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Ruiu, S" sort="Ruiu, S" uniqKey="Ruiu S" first="S." last="Ruiu">S. Ruiu</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Corsini, G U" sort="Corsini, G U" uniqKey="Corsini G" first="G. U." last="Corsini">G. U. Corsini</name><affiliation><mods:affiliation>Institute of Pharmacology, Medical School, Pisa, Italy</mods:affiliation></affiliation></author><author><name sortKey="Vaccari, A" sort="Vaccari, A" uniqKey="Vaccari A" first="A." last="Vaccari">A. Vaccari</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:416F953064520C81F6066BE7F86493579150BEAA</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1016/0006-8993(92)90677-2</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-MQFT8ZSC-P/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002734</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002734</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes</title><author><name sortKey="Del Zompo, M" sort="Del Zompo, M" uniqKey="Del Zompo M" first="M." last="Del Zompo">M. Del Zompo</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Piccardi, M P" sort="Piccardi, M P" uniqKey="Piccardi M" first="M. P." last="Piccardi">M. P. Piccardi</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Ruiu, S" sort="Ruiu, S" uniqKey="Ruiu S" first="S." last="Ruiu">S. Ruiu</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author><author><name sortKey="Corsini, G U" sort="Corsini, G U" uniqKey="Corsini G" first="G. U." last="Corsini">G. U. Corsini</name><affiliation><mods:affiliation>Institute of Pharmacology, Medical School, Pisa, Italy</mods:affiliation></affiliation></author><author><name sortKey="Vaccari, A" sort="Vaccari, A" uniqKey="Vaccari A" first="A." last="Vaccari">A. Vaccari</name><affiliation><mods:affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="ISSN">0006-8993</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">571</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="354">354</biblScope><biblScope unit="page" to="357">357</biblScope></imprint><idno type="ISSN">0006-8993</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-8993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding site</term><term>Mouse</term><term>N-Methyl-4-penylpyridinium ion</term><term>Striatum</term><term>Vesicle</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adenosine triphosphate</term><term>Appropriate assay conditions</term><term>Assay</term><term>Binding site</term><term>Binding sites</term><term>Brain area</term><term>Brain research</term><term>Chromaffin granules</term><term>Chronic parkinsonism</term><term>Dopamine</term><term>Dopaminergic neurons</term><term>High affinity</term><term>Ligand program</term><term>Monoamine</term><term>Monoamine oxidase</term><term>Mouse brain</term><term>Mouse brains</term><term>Mouse striatal membranes</term><term>Mouse striatum</term><term>Mptp</term><term>Pharmacol</term><term>Potent displacers</term><term>Present assay conditions</term><term>Right striatum</term><term>Specific binding</term><term>Striatal</term><term>Striatum</term><term>Subcellular distribution</term><term>Synaptic vesicles</term><term>Synaptosomal fraction</term><term>Toxic metabolite</term><term>Vesicle</term><term>Vesicular monoamine transporter</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>dopamine</json:string><json:string>mptp</json:string><json:string>binding sites</json:string><json:string>striatal</json:string><json:string>striatum</json:string><json:string>monoamine</json:string><json:string>vesicle</json:string><json:string>specific binding</json:string><json:string>pharmacol</json:string><json:string>mouse brain</json:string><json:string>assay</json:string><json:string>high affinity</json:string><json:string>dopaminergic neurons</json:string><json:string>potent displacers</json:string><json:string>brain research</json:string><json:string>vesicular monoamine transporter</json:string><json:string>mouse striatum</json:string><json:string>synaptosomal fraction</json:string><json:string>present assay conditions</json:string><json:string>ligand program</json:string><json:string>right striatum</json:string><json:string>subcellular distribution</json:string><json:string>appropriate assay conditions</json:string><json:string>brain area</json:string><json:string>toxic metabolite</json:string><json:string>binding site</json:string><json:string>mouse brains</json:string><json:string>adenosine triphosphate</json:string><json:string>synaptic vesicles</json:string><json:string>mouse striatal membranes</json:string><json:string>monoamine oxidase</json:string><json:string>chromaffin granules</json:string><json:string>chronic parkinsonism</json:string></teeft></keywords><subject><json:item><lang><json:string>eng</json:string></lang><value>N-Methyl-4-penylpyridinium ion</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Binding site</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Vesicle</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Striatum</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Mouse</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Short communication</json:string></originalGenre><qualityIndicators><score>5.795</score><pdfWordCount>2559</pdfWordCount><pdfCharCount>15289</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>4</pdfPageCount><pdfPageSize>576 x 828 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>103</abstractWordCount><abstractCharCount>749</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes</title><pii><json:string>0006-8993(92)90677-2</json:string></pii><genre><json:string>brief-communication</json:string></genre><host><title>Brain Research</title><language><json:string>unknown</json:string></language><publicationDate>1992</publicationDate><issn><json:string>0006-8993</json:string></issn><pii><json:string>S0006-8993(00)X0670-1</json:string></pii><volume>571</volume><issue>2</issue><pages><first>354</first><last>357</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1992</json:string></date><geogName></geogName><orgName><json:string>Department of Neuroscience</json:string><json:string>University of Cagliari</json:string><json:string>Biochem</json:string><json:string>US Department of Health, Education and Welfare</json:string><json:string>Neurochem</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>A. Vaccari</json:string><json:string>S. Ruiu</json:string><json:string>J. Biol</json:string><json:string>M. Del</json:string><json:string>Brain Research</json:string><json:string>Via Porcell</json:string><json:string>B.B.Brodie</json:string><json:string>J. Pharmacol</json:string><json:string>M.P. Piccardi</json:string></persName><placeName><json:string>Cagliari</json:string><json:string>Pisa</json:string><json:string>MD</json:string><json:string>Chiba</json:string><json:string>Rockville</json:string><json:string>Italy</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Lowry et al.</json:string><json:string>Whittaker et al.</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-MQFT8ZSC-P</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - neurosciences</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - neurology & neurosurgery</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Developmental Biology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Clinical Neurology</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Molecular Biology</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Neuroscience</json:string><json:string>3 - General Neuroscience</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - vertebres: systeme nerveux et organes des sens</json:string></inist></categories><publicationDate>1992</publicationDate><author><json:item><name>M. Del Zompo</name><affiliations><json:string>Department of Neuroscience, University of Cagliari, Cagliari, Italy</json:string></affiliations></json:item><json:item><name>M.P. Piccardi</name><affiliations><json:string>Department of Neuroscience, University of Cagliari, Cagliari, Italy</json:string></affiliations></json:item><json:item><name>S. Ruiu</name><affiliations><json:string>Department of Neuroscience, University of Cagliari, Cagliari, Italy</json:string></affiliations></json:item><json:item><name>G.U. Corsini</name><affiliations><json:string>Institute of Pharmacology, Medical School, Pisa, Italy</json:string></affiliations></json:item><json:item><name>A. Vaccari</name><affiliations><json:string>Department of Neuroscience, University of Cagliari, Cagliari, Italy</json:string></affiliations></json:item></author><articleId><json:string>92906772</json:string></articleId><arkIstex>ark:/67375/6H6-MQFT8ZSC-P</arkIstex><abstract>Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</abstract><pmid><json:string>1611504</json:string></pmid><serie><title>Proc. Natl. Acad. Sci. U.S.A.</title><language><json:string>unknown</json:string></language><volume>82</volume><pages><first>2173</first><last>2177</last></pages></serie><copyrightDate>1992</copyrightDate><doi><json:string>10.1016/0006-8993(92)90677-2</json:string></doi><id>416F953064520C81F6066BE7F86493579150BEAA</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-MQFT8ZSC-P/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-MQFT8ZSC-P/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-MQFT8ZSC-P/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1992 Elsevier Science Publishers B.V. All rights reserved</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1992</date></publicationStmt><notesStmt><note type="brief-communication" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes</title><author xml:id="author-0000"><persName><forename type="first">M.</forename><surname>Del Zompo</surname></persName><note type="correspondence"><p>Correspondence: M. Del Zompo, Department of Neuroscience, ‘B.B.Brodie’, University ofCagliari, Via Porcell, 4, I-09124 Cagliari, Italy.</p></note><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation></author><author xml:id="author-0001"><persName><forename type="first">M.P.</forename><surname>Piccardi</surname></persName><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation></author><author xml:id="author-0002"><persName><forename type="first">S.</forename><surname>Ruiu</surname></persName><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation></author><author xml:id="author-0003"><persName><forename type="first">G.U.</forename><surname>Corsini</surname></persName><affiliation>Institute of Pharmacology, Medical School, Pisa, Italy</affiliation></author><author xml:id="author-0004"><persName><forename type="first">A.</forename><surname>Vaccari</surname></persName><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation></author><idno type="istex">416F953064520C81F6066BE7F86493579150BEAA</idno><idno type="ark">ark:/67375/6H6-MQFT8ZSC-P</idno><idno type="DOI">10.1016/0006-8993(92)90677-2</idno><idno type="PII">0006-8993(92)90677-2</idno><idno type="article-id">92906772</idno></analytic><monogr><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="pISSN">0006-8993</idno><idno type="PII">S0006-8993(00)X0670-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992"></date><biblScope unit="volume">571</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="354">354</biblScope><biblScope unit="page" to="357">357</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1992</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>N-Methyl-4-penylpyridinium ion</term></item><item><term>Binding site</term></item><item><term>Vesicle</term></item><item><term>Striatum</term></item><item><term>Mouse</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-MQFT8ZSC-P/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="sco"><item-info><jid>BRES</jid><aid>92906772</aid><ce:pii>0006-8993(92)90677-2</ce:pii><ce:doi>10.1016/0006-8993(92)90677-2</ce:doi><ce:copyright type="unknown" year="1992">Elsevier Science Publishers B.V. All rights reserved</ce:copyright></item-info><head><ce:title>Characterization of a putatively vesicular binding site for [<ce:sup loc="pre">3</ce:sup>H]MPP<ce:sup loc="post">+</ce:sup> in mouse striatal membranes</ce:title><ce:author-group><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Del Zompo</ce:surname><ce:cross-ref refid="cor1"><ce:sup loc="post">*</ce:sup></ce:cross-ref><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.P.</ce:given-name><ce:surname>Piccardi</ce:surname><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>Ruiu</ce:surname><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>G.U.</ce:given-name><ce:surname>Corsini</ce:surname><ce:cross-ref refid="aff2"><ce:sup loc="post">2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Vaccari</ce:surname><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:label>a</ce:label><ce:textfn>Department of Neuroscience, University of Cagliari, Cagliari, Italy</ce:textfn></ce:affiliation><ce:affiliation id="aff2"><ce:label>b</ce:label><ce:textfn>Institute of Pharmacology, Medical School, Pisa, Italy</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>*</ce:label><ce:text><ce:italic>Correspondence:</ce:italic> M. Del Zompo, Department of Neuroscience, ‘B.B.Brodie’, University ofCagliari, Via Porcell, 4, I-09124 Cagliari, Italy.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="29" month="10" year="1991"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">[<ce:sup loc="pre">3</ce:sup>H] <ce:italic>N</ce:italic>-methyl-4-phenylpyridinium ion (MPP<ce:sup loc="post">+</ce:sup>) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (<ce:italic>B</ce:italic><ce:inf loc="post">max</ce:inf> = 168 ± 15fmol/mg protein, <ce:italic>K</ce:italic><ce:inf loc="post">D</ce:inf> = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [<ce:sup loc="pre">3</ce:sup>H]MPP<ce:sup loc="post">+</ce:sup> sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [<ce:sup loc="pre">3</ce:sup>H]MPP<ce:sup loc="post">+</ce:sup>, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg<ce:sup loc="post">2+</ce:sup>-ions did not affect [<ce:sup loc="pre">3</ce:sup>H]MPP<ce:sup loc="post">+</ce:sup> binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword" xml:lang="en"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>N</ce:italic>-Methyl-4-penylpyridinium ion</ce:text></ce:keyword><ce:keyword><ce:text>Binding site</ce:text></ce:keyword><ce:keyword><ce:text>Vesicle</ce:text></ce:keyword><ce:keyword><ce:text>Striatum</ce:text></ce:keyword><ce:keyword><ce:text>Mouse</ce:text></ce:keyword></ce:keywords></head><tail view="all"><ce:bibliography view="all" id="bibliography.0010"><ce:section-title>Reference</ce:section-title><ce:bibliography-sec id="bibliography-sec.0010"><ce:bib-reference id="bib1"><ce:label>1</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Barnes</ce:surname><ce:given-name>N.M.</ce:given-name></sb:author><sb:author><ce:surname>Cheng</ce:surname><ce:given-name>C.H.K.</ce:given-name></sb:author><sb:author><ce:surname>Costall</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Naylor</ce:surname><ce:given-name>R.J.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The relative abilities of MPTP and MPP<ce:sup loc="post">+</ce:sup> to compete with<ce:sup loc="pre">3</ce:sup>H-dopamine for the rat and marmoset striatal dopamine uptake site</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Biochem. Pharmacol.</sb:maintitle></sb:title><sb:volume-nr>39</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>809</sb:first-page><sb:last-page>811</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib2"><ce:label>2</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Bochetta</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Piccardi</ce:surname><ce:given-name>M.P.</ce:given-name></sb:author><sb:author><ce:surname>Del Zompo</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Pintus</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>Corsini</ce:surname><ce:given-name>G.U.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine: correspondence of its binding sites to monoamine oxidase in rat brain, and inhibition of dopamine oxidative deamination in vivo and in vitro</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Neurochem.</sb:maintitle></sb:title><sb:volume-nr>45</sb:volume-nr></sb:series><sb:date>1985</sb:date></sb:issue><sb:pages><sb:first-page>673</sb:first-page><sb:last-page>676</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib3"><ce:label>3</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Bradbury</ce:surname><ce:given-name>A.J.</ce:given-name></sb:author><sb:author><ce:surname>Costall</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Domeney</ce:surname><ce:given-name>A.M.</ce:given-name></sb:author><sb:author><ce:surname>Jenner</ce:surname><ce:given-name>P.J.</ce:given-name></sb:author><sb:author><ce:surname>Marsden</ce:surname><ce:given-name>C.D.</ce:given-name></sb:author><sb:author><ce:surname>Naylor</ce:surname><ce:given-name>R.J.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>1-Methyl-4-phenylpyridine is neurotoxic to the nigrostriatal dopamine pathway</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle></sb:title><sb:volume-nr>319</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>56</sb:first-page><sb:last-page>57</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib4"><ce:label>4</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Chiba</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Trevor</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Castagnoli</ce:surname><ce:given-name>N.</ce:given-name><ce:suffix>Jr.</ce:suffix></sb:author></sb:authors><sb:title><sb:maintitle>Metabolism of the neurotoxic tertiary amine, MPTP, by brain monoamine oxidase</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Biochem. Biophys. Res. Commun.</sb:maintitle></sb:title><sb:volume-nr>120</sb:volume-nr></sb:series><sb:date>1984</sb:date></sb:issue><sb:pages><sb:first-page>574</sb:first-page><sb:last-page>578</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib5"><ce:label>5</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>D'Amato</ce:surname><ce:given-name>R.J.</ce:given-name></sb:author><sb:author><ce:surname>Lipman</ce:surname><ce:given-name>Z.P.</ce:given-name></sb:author><sb:author><ce:surname>Snyder</ce:surname><ce:given-name>S.H.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Selectivity of parkinsonian neurotoxin MPTP: toxic metabolite MPP<ce:sup loc="post">+</ce:sup> binds to neuromelanin</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Science</sb:maintitle></sb:title><sb:volume-nr>231</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>987</sb:first-page><sb:last-page>989</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib6"><ce:label>6</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Daniels</ce:surname><ce:given-name>A.J.</ce:given-name></sb:author><sb:author><ce:surname>Reinhard</ce:surname><ce:given-name>J.F.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Energy-driven uptake of the neurotoxin 1-methyl-4-phenylpyridinium into chromaffin granules via the catecholamine transporter</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Biol. Chem.</sb:maintitle></sb:title><sb:volume-nr>263</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>5034</sb:first-page><sb:last-page>5036</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib7"><ce:label>7</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Davis</ce:surname><ce:given-name>G.C.</ce:given-name></sb:author><sb:author><ce:surname>Williams</ce:surname><ce:given-name>A.C.</ce:given-name></sb:author><sb:author><ce:surname>Markey</ce:surname><ce:given-name>S.P.</ce:given-name></sb:author><sb:author><ce:surname>Ebert</ce:surname><ce:given-name>M.H.</ce:given-name></sb:author><sb:author><ce:surname>Caine</ce:surname><ce:given-name>E.D.</ce:given-name></sb:author><sb:author><ce:surname>Riechert</ce:surname><ce:given-name>C.M.</ce:given-name></sb:author><sb:author><ce:surname>Kopin</ce:surname><ce:given-name>I.J.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Chronic parkinsonism secondary to intravenous injection of meperidine analogues</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Psychiatr. Res.</sb:maintitle></sb:title><sb:volume-nr>1</sb:volume-nr></sb:series><sb:date>1979</sb:date></sb:issue><sb:pages><sb:first-page>249</sb:first-page><sb:last-page>254</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib8"><ce:label>8</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Del Zompo</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Bernardi</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>Maggio</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Piccardi</ce:surname><ce:given-name>M.P.</ce:given-name></sb:author><sb:author><ce:surname>Johannssen</ce:surname><ce:given-name>J.N.</ce:given-name></sb:author><sb:author><ce:surname>Corsini</ce:surname><ce:given-name>G.U.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>High affinity binding sites for 1-methyl-4-phenyl-pyridinium ion (MPP<ce:sup loc="post">+</ce:sup>) are present in mouse brain</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Eur. J. Pharmacol.</sb:maintitle></sb:title><sb:volume-nr>129</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>87</sb:first-page><sb:last-page>92</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib9"><ce:label>9</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Del Zompo</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Piccardi</ce:surname><ce:given-name>M.P.</ce:given-name></sb:author><sb:author><ce:surname>Bernardi</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>Bonuccelli</ce:surname><ce:given-name>U.</ce:given-name></sb:author><sb:author><ce:surname>Corsini</ce:surname><ce:given-name>G.U.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Involvement of monoamine oxidase enzymes in the action of 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine, a selective neurotoxin, in the squirrel monkey: binding and biochemical studies</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Brain Research</sb:maintitle></sb:title><sb:volume-nr>378</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>320</sb:first-page><sb:last-page>324</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib10"><ce:label>10</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Del Zompo</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Ruiu</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>Maggio</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Piccardi</ce:surname><ce:given-name>M.P.</ce:given-name></sb:author><sb:author><ce:surname>Corsini</ce:surname><ce:given-name>G.U.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>(<ce:sup loc="pre">3</ce:sup>H)1-methyl-4-phenyl-2.3-dihydropyridinium ion binding sites in mouse brain: pharmacological and biological characterization</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Neurochem.</sb:maintitle></sb:title><sb:volume-nr>54</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>1905</sb:first-page><sb:last-page>1910</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib11"><ce:label>11</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Hallman</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Lange</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Olson</ce:surname><ce:given-name>L.</ce:given-name></sb:author><sb:author><ce:surname>Stromberg</ce:surname><ce:given-name>I.</ce:given-name></sb:author><sb:author><ce:surname>Jonsson</ce:surname><ce:given-name>J.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Neurochemical and histochemical characterization of neurotoxin effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on brain catecholamine neurones in the mouse</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Neurochem.</sb:maintitle></sb:title><sb:volume-nr>44</sb:volume-nr></sb:series><sb:date>1985</sb:date></sb:issue><sb:pages><sb:first-page>117</sb:first-page><sb:last-page>127</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib12"><ce:label>12</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Heikkila</ce:surname><ce:given-name>R.E.</ce:given-name></sb:author><sb:author><ce:surname>Cabbat</ce:surname><ce:given-name>F.S.</ce:given-name></sb:author><sb:author><ce:surname>Mytilineou</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Studies on the capacity of mazindol and dita to act as uptake inhibitors or releasing agents for (<ce:sup loc="pre">3</ce:sup>H) biogenic amines in rat brain tissue slices</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Eur. J. Pharmacol.</sb:maintitle></sb:title><sb:volume-nr>45</sb:volume-nr></sb:series><sb:date>1977</sb:date></sb:issue><sb:pages><sb:first-page>329</sb:first-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib13"><ce:label>13</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Henry</ce:surname><ce:given-name>J.-P.</ce:given-name></sb:author><sb:author><ce:surname>Scherman</ce:surname><ce:given-name>D.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Radioligands of the vesicular monoamine transporter and their use as marker of monoamine storage vesicles</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Biochem. Pharmacol.</sb:maintitle></sb:title><sb:volume-nr>38</sb:volume-nr></sb:series><sb:date>1989</sb:date></sb:issue><sb:pages><sb:first-page>2395</sb:first-page><sb:last-page>2404</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib14"><ce:label>14</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Javitch</ce:surname><ce:given-name>J.A.</ce:given-name></sb:author><sb:author><ce:surname>D'Amato</ce:surname><ce:given-name>R.J.</ce:given-name></sb:author><sb:author><ce:surname>Strittmatter</ce:surname><ce:given-name>S.M.</ce:given-name></sb:author><sb:author><ce:surname>Snyder</ce:surname><ce:given-name>S.H.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Parkinsonism-inducing neurotoxin, <ce:italic>N</ce:italic>-methyl-4-phenyl-1,2,3,6-tetrahydropyridine: uptake of the metabolite <ce:italic>N</ce:italic>-methyl-4-phenylpyridine by dopamine neurons explains selective toxicity</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:book-series><sb:series><sb:title><sb:maintitle>Proc. Natl. Acad. Sci. U.S.A.</sb:maintitle></sb:title><sb:volume-nr>82</sb:volume-nr></sb:series></sb:book-series><sb:date>1985</sb:date></sb:edited-book><sb:pages><sb:first-page>2173</sb:first-page><sb:last-page>2177</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib15"><ce:label>15</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Langston</ce:surname><ce:given-name>J.W.</ce:given-name></sb:author><sb:author><ce:surname>Ballard</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Tetrud</ce:surname><ce:given-name>J.W.</ce:given-name></sb:author><sb:author><ce:surname>Irwin</ce:surname><ce:given-name>I.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Chronic parkinsonism in humans due to a product of meperidine-analog synthesis</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Science</sb:maintitle></sb:title><sb:volume-nr>219</sb:volume-nr></sb:series><sb:date>1983</sb:date></sb:issue><sb:pages><sb:first-page>979</sb:first-page><sb:last-page>980</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib16"><ce:label>16</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Markey</ce:surname><ce:given-name>S.P.</ce:given-name></sb:author><sb:author><ce:surname>Johannessen</ce:surname><ce:given-name>J.N.</ce:given-name></sb:author><sb:author><ce:surname>Chiueh</ce:surname><ce:given-name>C.C.</ce:given-name></sb:author><sb:author><ce:surname>Burns</ce:surname><ce:given-name>R.S.</ce:given-name></sb:author><sb:author><ce:surname>Herkenham</ce:surname><ce:given-name>M.A.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Intraneuronal generation of a pyridinium metabolite may cause drug-induced parkinsonism</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle></sb:title><sb:volume-nr>311</sb:volume-nr></sb:series><sb:date>1984</sb:date></sb:issue><sb:pages><sb:first-page>464</sb:first-page><sb:last-page>467</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib17"><ce:label>17</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Munson</ce:surname><ce:given-name>P.J.</ce:given-name></sb:author><sb:author><ce:surname>Rodbard</ce:surname><ce:given-name>D.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>LIGAND: a versatile computerized approach for the characterization of ligand-binding systems</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Anal. Biochem.</sb:maintitle></sb:title><sb:volume-nr>107</sb:volume-nr></sb:series><sb:date>1980</sb:date></sb:issue><sb:pages><sb:first-page>220</sb:first-page><sb:last-page>239</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib18"><ce:label>18</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Peterson</ce:surname><ce:given-name>A.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>A simplification of the protein assay method of Lowry et al. which is more generally applicable</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Anal. Biochem.</sb:maintitle></sb:title><sb:volume-nr>83</sb:volume-nr></sb:series><sb:date>1977</sb:date></sb:issue><sb:pages><sb:first-page>346</sb:first-page><sb:last-page>356</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib19"><ce:label>19</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Rainbow</ce:surname><ce:given-name>T.C.</ce:given-name></sb:author><sb:author><ce:surname>Parsons</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Wieczorek</ce:surname><ce:given-name>C.M.</ce:given-name></sb:author><sb:author><ce:surname>Manaker</ce:surname><ce:given-name>S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Localization in rat brain of binding sites for parkinsonian toxin MPTP; similarities with [<ce:sup loc="pre">3</ce:sup>H]-pargyline binding to monoamine oxidase</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Brain Research</sb:maintitle></sb:title><sb:volume-nr>330</sb:volume-nr></sb:series><sb:date>1985</sb:date></sb:issue><sb:pages><sb:first-page>337</sb:first-page><sb:last-page>342</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib20"><ce:label>20</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Reinhard</ce:surname><ce:given-name>J.F.</ce:given-name><ce:suffix>Jr.</ce:suffix></sb:author><sb:author><ce:surname>Daniels</ce:surname><ce:given-name>A.J.</ce:given-name></sb:author><sb:author><ce:surname>Viveros</ce:surname><ce:given-name>O.H.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Potentiation by reserpine and tetrabenazine of brain catecholamine depletions by MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) in the mouse, evidence for subcellular sequestration as basis for cellular resistence to the toxicant</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Neurosci. Lett.</sb:maintitle></sb:title><sb:volume-nr>90</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>349</sb:first-page><sb:last-page>353</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib21"><ce:label>21</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Scherman</ce:surname><ce:given-name>D.</ce:given-name></sb:author><sb:author><ce:surname>Darchen</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>Desnos</ce:surname><ce:given-name>C.</ce:given-name></sb:author><sb:author><ce:surname>Henry</ce:surname><ce:given-name>J.P.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>1-Methyl-4-phenylpyridinium is a substrate of the vesicular monoamine uptake system of chromaffin granules</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Eur. J. Pharmacol.</sb:maintitle></sb:title><sb:volume-nr>146</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>359</sb:first-page><sb:last-page>360</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib22"><ce:label>22</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Schultz</ce:surname><ce:given-name>W.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>MPTP-Induced parkinsonism in monkeys: mechanism of action, selectivity and pathophysiology</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Gen. Pharmacol.</sb:maintitle></sb:title><sb:volume-nr>19</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>153</sb:first-page><sb:last-page>161</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib23"><ce:label>23</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Slotnick</ce:surname><ce:given-name>B.M.</ce:given-name></sb:author><sb:author><ce:surname>Leonard</ce:surname><ce:given-name>C.M.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>A Stereotaxic Atlas of the Albino Mouse Forebrain</sb:maintitle></sb:title></sb:contribution><sb:host><sb:book><sb:date>1975</sb:date><sb:publisher><sb:name>US Department of Health, Education and Welfare</sb:name><sb:location>Rockville, MD</sb:location></sb:publisher></sb:book></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib24"><ce:label>24</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Vaccari</ce:surname><ce:given-name>A.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>High affinity binding of<ce:sup loc="pre">3</ce:sup>H-tyramine in the central nervous system</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Br. J. Pharmacol.</sb:maintitle></sb:title><sb:volume-nr>89</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>15</sb:first-page><sb:last-page>25</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib25"><ce:label>25</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Whittaker</ce:surname><ce:given-name>W.P.</ce:given-name></sb:author><sb:author><ce:surname>Michaelson</ce:surname><ce:given-name>J.A.</ce:given-name></sb:author><sb:author><ce:surname>Kirkland</ce:surname><ce:given-name>R.J.A.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The separation of synaptic vesicles from nerve-ending particles (‘synaptosomes’)</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Biochem. J.</sb:maintitle></sb:title><sb:volume-nr>90</sb:volume-nr></sb:series><sb:date>1964</sb:date></sb:issue><sb:pages><sb:first-page>293</sb:first-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Characterization of a putatively vesicular binding site for [</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Del Zompo</namePart><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation><description>Correspondence: M. Del Zompo, Department of Neuroscience, ‘B.B.Brodie’, University ofCagliari, Via Porcell, 4, I-09124 Cagliari, Italy.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.P.</namePart><namePart type="family">Piccardi</namePart><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Ruiu</namePart><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.U.</namePart><namePart type="family">Corsini</namePart><affiliation>Institute of Pharmacology, Medical School, Pisa, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Vaccari</namePart><affiliation>Department of Neuroscience, University of Cagliari, Cagliari, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: [3H] N-methyl-4-phenylpyridinium ion (MPP+) binds with fully reversible, high affinity process to a population of sites mainly localized in the mouse striatum (Bmax = 168 ± 15fmol/mg protein, KD = 1.4 ± 0.4nM). The majority of specifically-bound radioactivity was localized in the synaptosomal fraction. Unilateral, striatal denervation with 6-hydroxydopamine (6-OHDA) markedly (by 65–70%) decreased the number of [3H]MPP+ sites. Besides dopamine, the vesicular markers tyramine, tetrabenazine and reserpine inhibited [3H]MPP+, while mazindol was a poor displacer. Adenosine triphosphate (ATP) and Mg2+-ions did not affect [3H]MPP+ binding. It is concluded that these sites may represent a marker of striatal storage vesicles for dopamine.</abstract><subject lang="en"><genre>Keywords</genre><topic>N-Methyl-4-penylpyridinium ion</topic><topic>Binding site</topic><topic>Vesicle</topic><topic>Striatum</topic><topic>Mouse</topic></subject><relatedItem type="host"><titleInfo><title>Brain Research</title></titleInfo><titleInfo type="abbreviated"><title>BRES</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0006-8993</identifier><identifier type="PII">S0006-8993(00)X0670-1</identifier><part><date>1992</date><detail type="volume"><number>571</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>181</start><end>360</end></extent><extent unit="pages"><start>354</start><end>357</end></extent></part></relatedItem><identifier type="istex">416F953064520C81F6066BE7F86493579150BEAA</identifier><identifier type="ark">ark:/67375/6H6-MQFT8ZSC-P</identifier><identifier type="DOI">10.1016/0006-8993(92)90677-2</identifier><identifier type="PII">0006-8993(92)90677-2</identifier><identifier type="ArticleID">92906772</identifier><accessCondition type="use and reproduction" contentType="copyright">©1992 Elsevier Science Publishers B.V. All rights reserved</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Elsevier Science Publishers B.V. All rights reserved, ©1992</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-MQFT8ZSC-P/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002734 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 002734 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:416F953064520C81F6066BE7F86493579150BEAA
|texte= Characterization of a putatively vesicular binding site for [3H]MPP+ in mouse striatal membranes
}}
| This area was generated with Dilib version V0.6.33. |  |