In vitro response of Blastocystis hominis against traditional Chinese medicine
Identifieur interne : 002727 ( Istex/Corpus ); précédent : 002726; suivant : 002728In vitro response of Blastocystis hominis against traditional Chinese medicine
Auteurs : L. Q. Yang ; Mulkit Singh ; E. H. Yap ; G. C. Ng ; H. X. Xu ; K. Y. SimSource :
- Journal of Ethnopharmacology [ 0378-8741 ] ; 1996.
English descriptors
- Teeft :
- Active concentration, Blastocystis hominis, Cell count, Cell counts, Cell rate, Control group, Coptis chinensis, Crude extracts, Dead cells, Different drugs, Drug concentration, Herbal extracts, High inhibition, Hominis, Intact cells, Intestinal parasite, Light microscope, Metronidazole, Moderate inhibition, Percentage rate, Present study, Shanghai university, Traditional chinese medicine, Traditional medicine, Vacuolar forms, Zierdt.
Abstract
Abstract: This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for B. hominis.
Url:
DOI: 10.1016/S0378-8741(96)01471-7
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ISTEX:16E79D3D66F7909CC3CFCC268EC5E1501C5433A6Le document en format XML
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Fax: +65 7766872</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: micmulki@nus.sg</mods:affiliation></affiliation></author><author><name sortKey="Yap, E H" sort="Yap, E H" uniqKey="Yap E" first="E. H." last="Yap">E. H. Yap</name><affiliation><mods:affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Ng, G C" sort="Ng, G C" uniqKey="Ng G" first="G. C." last="Ng">G. C. 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Yang</name><affiliation><mods:affiliation>Department of Parasitology, Shanghai University of Traditional Chinese Medicine, 530 Ling Ling Road, Shanghai 200032, P.R. China</mods:affiliation></affiliation></author><author><name sortKey="Singh, Mulkit" sort="Singh, Mulkit" uniqKey="Singh M" first="Mulkit" last="Singh">Mulkit Singh</name><affiliation><mods:affiliation>Corresponding author. Fax: +65 7766872</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: micmulki@nus.sg</mods:affiliation></affiliation></author><author><name sortKey="Yap, E H" sort="Yap, E H" uniqKey="Yap E" first="E. H." last="Yap">E. H. Yap</name><affiliation><mods:affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Ng, G C" sort="Ng, G C" uniqKey="Ng G" first="G. C." last="Ng">G. C. Ng</name><affiliation><mods:affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Xu, H X" sort="Xu, H X" uniqKey="Xu H" first="H. X." last="Xu">H. X. Xu</name><affiliation><mods:affiliation>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</mods:affiliation></affiliation></author><author><name sortKey="Sim, K Y" sort="Sim, K Y" uniqKey="Sim K" first="K. Y." last="Sim">K. Y. Sim</name><affiliation><mods:affiliation>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Ethnopharmacology</title><title level="j" type="abbrev">JEP</title><idno type="ISSN">0378-8741</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">55</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="35">35</biblScope><biblScope unit="page" to="42">42</biblScope></imprint><idno type="ISSN">0378-8741</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-8741</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Active concentration</term><term>Blastocystis hominis</term><term>Cell count</term><term>Cell counts</term><term>Cell rate</term><term>Control group</term><term>Coptis chinensis</term><term>Crude extracts</term><term>Dead cells</term><term>Different drugs</term><term>Drug concentration</term><term>Herbal extracts</term><term>High inhibition</term><term>Hominis</term><term>Intact cells</term><term>Intestinal parasite</term><term>Light microscope</term><term>Metronidazole</term><term>Moderate inhibition</term><term>Percentage rate</term><term>Present study</term><term>Shanghai university</term><term>Traditional chinese medicine</term><term>Traditional medicine</term><term>Vacuolar forms</term><term>Zierdt</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for B. hominis.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>hominis</json:string><json:string>zierdt</json:string><json:string>metronidazole</json:string><json:string>active concentration</json:string><json:string>crude extracts</json:string><json:string>traditional chinese medicine</json:string><json:string>present study</json:string><json:string>control group</json:string><json:string>blastocystis hominis</json:string><json:string>high inhibition</json:string><json:string>cell count</json:string><json:string>moderate inhibition</json:string><json:string>cell counts</json:string><json:string>cell rate</json:string><json:string>drug concentration</json:string><json:string>shanghai university</json:string><json:string>percentage rate</json:string><json:string>coptis chinensis</json:string><json:string>dead cells</json:string><json:string>herbal extracts</json:string><json:string>light microscope</json:string><json:string>intact cells</json:string><json:string>intestinal parasite</json:string><json:string>vacuolar forms</json:string><json:string>different drugs</json:string><json:string>traditional medicine</json:string></teeft></keywords><author><json:item><name>L.Q. Yang</name><affiliations><json:string>Department of Parasitology, Shanghai University of Traditional Chinese Medicine, 530 Ling Ling Road, Shanghai 200032, P.R. China</json:string></affiliations></json:item><json:item><name>Mulkit Singh</name><affiliations><json:string>Corresponding author. Fax: +65 7766872</json:string><json:string>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</json:string><json:string>E-mail: micmulki@nus.sg</json:string></affiliations></json:item><json:item><name>E.H. Yap</name><affiliations><json:string>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</json:string></affiliations></json:item><json:item><name>G.C. Ng</name><affiliations><json:string>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</json:string></affiliations></json:item><json:item><name>H.X. Xu</name><affiliations><json:string>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</json:string></affiliations></json:item><json:item><name>K.Y. Sim</name><affiliations><json:string>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Blastocystis hominis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Drug response</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Traditional medicine</value></json:item></subject><arkIstex>ark:/67375/6H6-56J2J14J-4</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for B. hominis.</abstract><qualityIndicators><score>7.074</score><pdfWordCount>3178</pdfWordCount><pdfCharCount>16988</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>576 x 749 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>158</abstractWordCount><abstractCharCount>986</abstractCharCount><keywordCount>3</keywordCount></qualityIndicators><title>In vitro response of Blastocystis hominis against traditional Chinese medicine</title><pmid><json:string>9121165</json:string></pmid><pii><json:string>S0378-8741(96)01471-7</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Journal of Ethnopharmacology</title><language><json:string>unknown</json:string></language><publicationDate>1996</publicationDate><issn><json:string>0378-8741</json:string></issn><pii><json:string>S0378-8741(00)X0026-8</json:string></pii><volume>55</volume><issue>1</issue><pages><first>35</first><last>42</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1996</json:string></date><geogName></geogName><orgName><json:string>Shanghai University</json:string><json:string>Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, China</json:string><json:string>Singapore Received</json:string><json:string>Elsevier Science Ireland Ltd</json:string><json:string>Department of Microbiology, National University of Singapore</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>L.Q. 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Yang et al.</json:string><json:string>Dunn and Boreham (1991)</json:string><json:string>Pavanand et al., 1986</json:string><json:string>Ho et al., 1993</json:string><json:string>Garavelli et al., 1991</json:string><json:string>Fukamiya et al., 1987</json:string><json:string>O'Neill et al., 1987</json:string><json:string>Rustia and Shubik, 1972</json:string><json:string>Zierdt et al., 1983</json:string><json:string>Garavelli et al., 1990</json:string><json:string>Kain et al., 1987</json:string><json:string>Zierdt, 1991</json:string><json:string>Llbire et al., 1989</json:string><json:string>Jeddy and Farrington, 1991</json:string><json:string>Wright et al., 1988</json:string><json:string>Zierdt, 1989</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-56J2J14J-4</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - plant sciences</json:string><json:string>2 - pharmacology & pharmacy</json:string><json:string>2 - integrative & complementary medicine</json:string><json:string>2 - chemistry, medicinal</json:string></wos><scienceMetrix><json:string>1 - natural sciences</json:string><json:string>2 - chemistry</json:string><json:string>3 - medicinal & biomolecular chemistry</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Pharmacology, Toxicology and Pharmaceutics</json:string><json:string>3 - Drug Discovery</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Pharmacology, Toxicology and Pharmaceutics</json:string><json:string>3 - Pharmacology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - genetique des eucaryotes. evolution biologique et moleculaire</json:string></inist></categories><publicationDate>1996</publicationDate><copyrightDate>1996</copyrightDate><doi><json:string>10.1016/S0378-8741(96)01471-7</json:string></doi><id>16E79D3D66F7909CC3CFCC268EC5E1501C5433A6</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-56J2J14J-4/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-56J2J14J-4/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-56J2J14J-4/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">In vitro response of Blastocystis hominis against traditional Chinese medicine</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1996</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Research paper</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">In vitro response of Blastocystis hominis against traditional Chinese medicine</title><author xml:id="author-0000"><persName><forename type="first">L.Q.</forename><surname>Yang</surname></persName><affiliation>Department of Parasitology, Shanghai University of Traditional Chinese Medicine, 530 Ling Ling Road, Shanghai 200032, P.R. China</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Mulkit</forename><surname>Singh</surname></persName><email>micmulki@nus.sg</email><affiliation>Corresponding author. Fax: +65 7766872</affiliation><affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation></author><author xml:id="author-0002"><persName><forename type="first">E.H.</forename><surname>Yap</surname></persName><affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation></author><author xml:id="author-0003"><persName><forename type="first">G.C.</forename><surname>Ng</surname></persName><affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation></author><author xml:id="author-0004"><persName><forename type="first">H.X.</forename><surname>Xu</surname></persName><affiliation>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation></author><author xml:id="author-0005"><persName><forename type="first">K.Y.</forename><surname>Sim</surname></persName><affiliation>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation></author><idno type="istex">16E79D3D66F7909CC3CFCC268EC5E1501C5433A6</idno><idno type="ark">ark:/67375/6H6-56J2J14J-4</idno><idno type="DOI">10.1016/S0378-8741(96)01471-7</idno><idno type="PII">S0378-8741(96)01471-7</idno></analytic><monogr><title level="j">Journal of Ethnopharmacology</title><title level="j" type="abbrev">JEP</title><idno type="pISSN">0378-8741</idno><idno type="PII">S0378-8741(00)X0026-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996"></date><biblScope unit="volume">55</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="35">35</biblScope><biblScope unit="page" to="42">42</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for B. hominis.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Blastocystis hominis</term></item><item><term>Drug response</term></item><item><term>Traditional medicine</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1996-08-15">Modified</change><change when="1996">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-56J2J14J-4/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JEP</jid><aid>96014717</aid><ce:pii>S0378-8741(96)01471-7</ce:pii><ce:doi>10.1016/S0378-8741(96)01471-7</ce:doi><ce:copyright type="unknown" year="1996"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Research paper</ce:textfn></ce:dochead><ce:title>In vitro response of <ce:italic>Blastocystis hominis</ce:italic> against traditional Chinese medicine</ce:title><ce:author-group><ce:author><ce:given-name>L.Q.</ce:given-name><ce:surname>Yang</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Mulkit</ce:given-name><ce:surname>Singh</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:e-address>micmulki@nus.sg</ce:e-address></ce:author><ce:author><ce:given-name>E.H.</ce:given-name><ce:surname>Yap</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>G.C.</ce:given-name><ce:surname>Ng</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>H.X.</ce:given-name><ce:surname>Xu</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>K.Y.</ce:given-name><ce:surname>Sim</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Parasitology, Shanghai University of Traditional Chinese Medicine, 530 Ling Ling Road, Shanghai 200032, P.R. China</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Corresponding author. Fax: +65 7766872</ce:text></ce:correspondence></ce:author-group><ce:date-received day="30" month="10" year="1995"></ce:date-received><ce:date-revised day="15" month="8" year="1996"></ce:date-revised><ce:date-accepted day="20" month="8" year="1996"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, <ce:italic>Blastocystis hominis</ce:italic> using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of <ce:italic>Coptis chinensis</ce:italic> (CC) and <ce:italic>Brucea javanica</ce:italic> (BJ) were found to be most active against <ce:italic>B. hominis</ce:italic>. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for <ce:italic>B. hominis</ce:italic>.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Blastocystis hominis</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Drug response</ce:text></ce:keyword><ce:keyword><ce:text>Traditional medicine</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>In vitro response of Blastocystis hominis against traditional Chinese medicine</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>In vitro response of</title></titleInfo><name type="personal"><namePart type="given">L.Q.</namePart><namePart type="family">Yang</namePart><affiliation>Department of Parasitology, Shanghai University of Traditional Chinese Medicine, 530 Ling Ling Road, Shanghai 200032, P.R. China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mulkit</namePart><namePart type="family">Singh</namePart><affiliation>Corresponding author. Fax: +65 7766872</affiliation><affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation><affiliation>E-mail: micmulki@nus.sg</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.H.</namePart><namePart type="family">Yap</namePart><affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.C.</namePart><namePart type="family">Ng</namePart><affiliation>Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.X.</namePart><namePart type="family">Xu</namePart><affiliation>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.Y.</namePart><namePart type="family">Sim</namePart><affiliation>Department of Chemistry, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><dateModified encoding="w3cdtf">1996-08-15</dateModified><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: This is the first in vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 μg/ml. The active concentration of BJ was 500 μg/ml. The active concentration of metronidazole (MD) was 10 μg/ml and this was taken as an active standard drug for B. hominis.</abstract><note type="content">Section title: Research paper</note><subject><genre>Keywords</genre><topic>Blastocystis hominis</topic><topic>Drug response</topic><topic>Traditional medicine</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Ethnopharmacology</title></titleInfo><titleInfo type="abbreviated"><title>JEP</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued></originInfo><identifier type="ISSN">0378-8741</identifier><identifier type="PII">S0378-8741(00)X0026-8</identifier><part><date>1996</date><detail type="volume"><number>55</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>80</end></extent><extent unit="pages"><start>35</start><end>42</end></extent></part></relatedItem><identifier type="istex">16E79D3D66F7909CC3CFCC268EC5E1501C5433A6</identifier><identifier type="ark">ark:/67375/6H6-56J2J14J-4</identifier><identifier type="DOI">10.1016/S0378-8741(96)01471-7</identifier><identifier type="PII">S0378-8741(96)01471-7</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-56J2J14J-4/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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