Association between HLA phenotype and HLA concentration in plasma or platelets
Identifieur interne : 002288 ( Istex/Corpus ); précédent : 002287; suivant : 002289Association between HLA phenotype and HLA concentration in plasma or platelets
Auteurs : K. J. Kao ; Juan C. Scornik ; William J. Riley ; Claudia F. McqueenSource :
- Human Immunology [ 0198-8859 ] ; 1988.
English descriptors
- Teeft :
- Antigen, Assay, Blood cells, Competitive immunoassay, Different blood components, Higher levels, Human plasma, Human serum, Lymphocyte, Major histocompatibility, Monoclonal antibody, Normal plasma, Phenotype, Phenotype association, Plasma, Plasma samples, Platelet, Platelet samples, Platelet surface, Precise quantitation, Present study, Quantitative analysis, Quantitative distribution, Room temperature, Significant association, Silver staining, Statistical analysis, Statistical comparison, Various phenotypes, Viral infection, Weight form, Whole blood.
Abstract
Abstract: To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 ± 1.67 μg/ml (±SD, n = 215) and 11.28 ± 4.65 fg/cell (±SD, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (p < 0.002) or 1.9 (p < 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (p < 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (p < 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.
Url:
DOI: 10.1016/0198-8859(88)90086-9
Links to Exploration step
ISTEX:6C60A538A621A619289C8A34EF9405776DA4FD4ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Association between HLA phenotype and HLA concentration in plasma or platelets</title><author><name sortKey="Kao, K J" sort="Kao, K J" uniqKey="Kao K" first="K. J." last="Kao">K. J. Kao</name><affiliation><mods:affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Scornik, Juan C" sort="Scornik, Juan C" uniqKey="Scornik J" first="Juan C." last="Scornik">Juan C. Scornik</name><affiliation><mods:affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Riley, William J" sort="Riley, William J" uniqKey="Riley W" first="William J." last="Riley">William J. Riley</name><affiliation><mods:affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Mcqueen, Claudia F" sort="Mcqueen, Claudia F" uniqKey="Mcqueen C" first="Claudia F." last="Mcqueen">Claudia F. 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Kao</name><affiliation><mods:affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Scornik, Juan C" sort="Scornik, Juan C" uniqKey="Scornik J" first="Juan C." last="Scornik">Juan C. Scornik</name><affiliation><mods:affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Riley, William J" sort="Riley, William J" uniqKey="Riley W" first="William J." last="Riley">William J. Riley</name><affiliation><mods:affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Mcqueen, Claudia F" sort="Mcqueen, Claudia F" uniqKey="Mcqueen C" first="Claudia F." last="Mcqueen">Claudia F. Mcqueen</name><affiliation><mods:affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Human Immunology</title><title level="j" type="abbrev">HIM</title><idno type="ISSN">0198-8859</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1988">1988</date><biblScope unit="volume">21</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="115">115</biblScope><biblScope unit="page" to="124">124</biblScope></imprint><idno type="ISSN">0198-8859</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0198-8859</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antigen</term><term>Assay</term><term>Blood cells</term><term>Competitive immunoassay</term><term>Different blood components</term><term>Higher levels</term><term>Human plasma</term><term>Human serum</term><term>Lymphocyte</term><term>Major histocompatibility</term><term>Monoclonal antibody</term><term>Normal plasma</term><term>Phenotype</term><term>Phenotype association</term><term>Plasma</term><term>Plasma samples</term><term>Platelet</term><term>Platelet samples</term><term>Platelet surface</term><term>Precise quantitation</term><term>Present study</term><term>Quantitative analysis</term><term>Quantitative distribution</term><term>Room temperature</term><term>Significant association</term><term>Silver staining</term><term>Statistical analysis</term><term>Statistical comparison</term><term>Various phenotypes</term><term>Viral infection</term><term>Weight form</term><term>Whole blood</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 ± 1.67 μg/ml (±SD, n = 215) and 11.28 ± 4.65 fg/cell (±SD, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (p < 0.002) or 1.9 (p < 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (p < 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (p < 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>platelet</json:string><json:string>phenotype</json:string><json:string>lymphocyte</json:string><json:string>whole blood</json:string><json:string>monoclonal antibody</json:string><json:string>present study</json:string><json:string>room temperature</json:string><json:string>assay</json:string><json:string>plasma</json:string><json:string>major histocompatibility</json:string><json:string>precise quantitation</json:string><json:string>significant association</json:string><json:string>higher levels</json:string><json:string>antigen</json:string><json:string>statistical analysis</json:string><json:string>plasma samples</json:string><json:string>platelet surface</json:string><json:string>normal plasma</json:string><json:string>statistical comparison</json:string><json:string>various phenotypes</json:string><json:string>blood cells</json:string><json:string>silver staining</json:string><json:string>platelet samples</json:string><json:string>viral infection</json:string><json:string>quantitative distribution</json:string><json:string>phenotype association</json:string><json:string>different blood components</json:string><json:string>quantitative analysis</json:string><json:string>weight form</json:string><json:string>human plasma</json:string><json:string>human serum</json:string><json:string>competitive immunoassay</json:string></teeft></keywords><author><json:item><name>K.J. Kao</name><affiliations><json:string>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</json:string></affiliations></json:item><json:item><name>Juan C. Scornik</name><affiliations><json:string>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</json:string></affiliations></json:item><json:item><name>William J. Riley</name><affiliations><json:string>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</json:string></affiliations></json:item><json:item><name>Claudia F. McQueen</name><affiliations><json:string>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>E.B. : Epstein-Barr</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>kD : kilo-Dalton</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>EIA : enzyme-linked immunoassay</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PRP : platelet-rich plasma</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HLA : class I molecules of major histocompatibility complex</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SD : standard deviation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SEM : standard error of mean</value></json:item></subject><arkIstex>ark:/67375/6H6-6SHJD2FR-M</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 ± 1.67 μg/ml (±SD, n = 215) and 11.28 ± 4.65 fg/cell (±SD, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (p > 0.002) or 1.9 (p > 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (p > 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (p > 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.</abstract><qualityIndicators><score>8.386</score><pdfWordCount>3806</pdfWordCount><pdfCharCount>23638</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>512 x 778 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>215</abstractWordCount><abstractCharCount>1379</abstractCharCount><keywordCount>7</keywordCount></qualityIndicators><title>Association between HLA phenotype and HLA concentration in plasma or platelets</title><pmid><json:string>3284856</json:string></pmid><pii><json:string>0198-8859(88)90086-9</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Human Immunology</title><language><json:string>unknown</json:string></language><publicationDate>1988</publicationDate><issn><json:string>0198-8859</json:string></issn><pii><json:string>S0198-8859(00)X0150-4</json:string></pii><volume>21</volume><issue>2</issue><pages><first>115</first><last>124</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1988</json:string></date><geogName></geogName><orgName><json:string>American Caucasians</json:string><json:string>Clinical Research Center</json:string><json:string>American Society</json:string><json:string>University of Florida</json:string><json:string>Department of Pathology, Box J</json:string><json:string>HL33345</json:string><json:string>NIH Grants HL35124</json:string></orgName><orgName_funder><json:string>HL33345</json:string><json:string>NIH Grants HL35124</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>E.B. Epstein-Barr</json:string><json:string>Their</json:string><json:string>Cathy Smith</json:string><json:string>The</json:string><json:string>Allison</json:string><json:string>William J. Riley</json:string><json:string>Juan C. Scornik</json:string><json:string>Kao</json:string></persName><placeName><json:string>Palo Alto</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>[4]</json:string><json:string>[ 18]</json:string><json:string>[7,8]</json:string><json:string>[23]</json:string><json:string>Kao et al.</json:string><json:string>[18]</json:string><json:string>[6]</json:string><json:string>Tekolf et al</json:string><json:string>[22]</json:string><json:string>[17]</json:string><json:string>[25,26]</json:string><json:string>[18,21]</json:string><json:string>[5,6]</json:string><json:string>[1]</json:string><json:string>[21]</json:string><json:string>[27]</json:string><json:string>K.J. Kao et al.</json:string><json:string>[3]</json:string><json:string>Aster et al</json:string><json:string>[5]</json:string><json:string>[26]</json:string><json:string>Pellegrino et al</json:string><json:string>[19,20]</json:string><json:string>[14]</json:string><json:string>[ 16]</json:string><json:string>[5,9,18]</json:string><json:string>[9]</json:string><json:string>[11,12]</json:string><json:string>[2]</json:string><json:string>[24]</json:string><json:string>Histocorapatibility and Immunogenetics, 1988</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-6SHJD2FR-M</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - immunology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - immunology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Immunology and Allergy</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1988</publicationDate><copyrightDate>1988</copyrightDate><doi><json:string>10.1016/0198-8859(88)90086-9</json:string></doi><id>6C60A538A621A619289C8A34EF9405776DA4FD4A</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6SHJD2FR-M/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6SHJD2FR-M/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-6SHJD2FR-M/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Association between HLA phenotype and HLA concentration in plasma or platelets</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1988</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Association between HLA phenotype and HLA concentration in plasma or platelets</title><author xml:id="author-0000"><persName><forename type="first">K.J.</forename><surname>Kao</surname></persName><note type="biography">Address reprint requests to Dr. Kao, Department of Pathology, Box J-275; JHMHC, University of Florida, Gainesville, Florida 32610</note><affiliation>Address reprint requests to Dr. Kao, Department of Pathology, Box J-275; JHMHC, University of Florida, Gainesville, Florida 32610</affiliation><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Juan C.</forename><surname>Scornik</surname></persName><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">William J.</forename><surname>Riley</surname></persName><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Claudia F.</forename><surname>McQueen</surname></persName><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation></author><idno type="istex">6C60A538A621A619289C8A34EF9405776DA4FD4A</idno><idno type="ark">ark:/67375/6H6-6SHJD2FR-M</idno><idno type="DOI">10.1016/0198-8859(88)90086-9</idno><idno type="PII">0198-8859(88)90086-9</idno></analytic><monogr><title level="j">Human Immunology</title><title level="j" type="abbrev">HIM</title><idno type="pISSN">0198-8859</idno><idno type="PII">S0198-8859(00)X0150-4</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1988"></date><biblScope unit="volume">21</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="115">115</biblScope><biblScope unit="page" to="124">124</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1988</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 ± 1.67 μg/ml (±SD, n = 215) and 11.28 ± 4.65 fg/cell (±SD, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (p < 0.002) or 1.9 (p < 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (p < 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (p < 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.</p></abstract><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>E.B.</term><term>Epstein-Barr</term></item><item><term>kD</term><term>kilo-Dalton</term></item><item><term>EIA</term><term>enzyme-linked immunoassay</term></item><item><term>PRP</term><term>platelet-rich plasma</term></item><item><term>HLA</term><term>class I molecules of major histocompatibility complex</term></item><item><term>SD</term><term>standard deviation</term></item><item><term>SEM</term><term>standard error of mean</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1988">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6SHJD2FR-M/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>HIM</jid><aid>88900869</aid><ce:pii>0198-8859(88)90086-9</ce:pii><ce:doi>10.1016/0198-8859(88)90086-9</ce:doi><ce:copyright type="unknown" year="1988"></ce:copyright></item-info><head><ce:title>Association between HLA phenotype and HLA concentration in plasma or platelets</ce:title><ce:author-group><ce:author><ce:given-name>K.J.</ce:given-name><ce:surname>Kao</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Juan C.</ce:given-name><ce:surname>Scornik</ce:surname></ce:author><ce:author><ce:given-name>William J.</ce:given-name><ce:surname>Riley</ce:surname></ce:author><ce:author><ce:given-name>Claudia F.</ce:given-name><ce:surname>McQueen</ce:surname></ce:author><ce:affiliation><ce:textfn>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Address reprint requests to Dr. Kao, Department of Pathology, Box J-275; JHMHC, University of Florida, Gainesville, Florida 32610</ce:text></ce:correspondence></ce:author-group><ce:date-received day="11" month="9" year="1987"></ce:date-received><ce:date-accepted day="23" month="11" year="1987"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 ± 1.67 <ce:italic>μg</ce:italic>/<ce:italic>ml</ce:italic> (±<ce:italic>SD</ce:italic>, n = 215) and 11.28 ± 4.65 <ce:italic>fg</ce:italic>/<ce:italic>cell</ce:italic> (±<ce:italic>SD</ce:italic>, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (<ce:italic>p</ce:italic> < 0.002) or 1.9 (<ce:italic>p</ce:italic> < 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (<ce:italic>p</ce:italic> < 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (<ce:italic>p</ce:italic> < 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>E.B.</ce:text><ce:keyword><ce:text>Epstein-Barr</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>kD</ce:text><ce:keyword><ce:text>kilo-Dalton</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>EIA</ce:text><ce:keyword><ce:text>enzyme-linked immunoassay</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PRP</ce:text><ce:keyword><ce:text>platelet-rich plasma</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>HLA</ce:text><ce:keyword><ce:text>class I molecules of major histocompatibility complex</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SD</ce:text><ce:keyword><ce:text>standard deviation</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SEM</ce:text><ce:keyword><ce:text>standard error of mean</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Association between HLA phenotype and HLA concentration in plasma or platelets</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Association between HLA phenotype and HLA concentration in plasma or platelets</title></titleInfo><name type="personal"><namePart type="given">K.J.</namePart><namePart type="family">Kao</namePart><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation><description>Address reprint requests to Dr. Kao, Department of Pathology, Box J-275; JHMHC, University of Florida, Gainesville, Florida 32610</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Juan C.</namePart><namePart type="family">Scornik</namePart><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William J.</namePart><namePart type="family">Riley</namePart><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Claudia F.</namePart><namePart type="family">McQueen</namePart><affiliation>From the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1988</dateIssued><copyrightDate encoding="w3cdtf">1988</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 ± 1.67 μg/ml (±SD, n = 215) and 11.28 ± 4.65 fg/cell (±SD, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (p < 0.002) or 1.9 (p < 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (p < 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (p < 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.</abstract><subject><genre>Abbreviations</genre><topic>E.B. : Epstein-Barr</topic><topic>kD : kilo-Dalton</topic><topic>EIA : enzyme-linked immunoassay</topic><topic>PRP : platelet-rich plasma</topic><topic>HLA : class I molecules of major histocompatibility complex</topic><topic>SD : standard deviation</topic><topic>SEM : standard error of mean</topic></subject><relatedItem type="host"><titleInfo><title>Human Immunology</title></titleInfo><titleInfo type="abbreviated"><title>HIM</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1988</dateIssued></originInfo><identifier type="ISSN">0198-8859</identifier><identifier type="PII">S0198-8859(00)X0150-4</identifier><part><date>1988</date><detail type="volume"><number>21</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>79</start><end>153</end></extent><extent unit="pages"><start>115</start><end>124</end></extent></part></relatedItem><identifier type="istex">6C60A538A621A619289C8A34EF9405776DA4FD4A</identifier><identifier type="ark">ark:/67375/6H6-6SHJD2FR-M</identifier><identifier type="DOI">10.1016/0198-8859(88)90086-9</identifier><identifier type="PII">0198-8859(88)90086-9</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6SHJD2FR-M/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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