The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes
Identifieur interne : 000C17 ( Istex/Corpus ); précédent : 000C16; suivant : 000C18The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes
Auteurs : Leiv Ose ; Turid Ose ; Kaare R. Norum ; Trond BergSource :
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism [ 0005-2760 ] ; 1980.
English descriptors
- Teeft :
- Acid phosphatase, Acta, Apolipoprotein, Beckman ultracentrifuge, Berg, Biol, Biophys, Bovine serum albumin, Centrifugation, Centrifuge tube, Chloroquine, Differential centrifugation, Enzyme activities, Hepatocytes, High density lipoproteins, Homogenate, Incubation, Incubation medium, Intracellular, Isopycnic, Isopycnic centrifugation, Light mitochondrial, Linear sucrose gradients, Lipoprotein, Lysosome, Major part, Marker enzymes, Mitochondrial, Phosphatase, Plasma cell membrane, Postnuclear fractions, Radioactivity, Speed centrifugation, Subcellular, Subcellular distribution, Subcellular fractions, Sucrose, Sucrose gradients, Sucrose solution, Unlabelled, Vivo.
Abstract
Abstract: The subcellular distribution of 125I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. 125I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound 125I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize 125I-labelled HDL. The 125I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of 125I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro 125I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of 125I-labelled HDL taken up by hepatocytes in vitro.
Url:
DOI: 10.1016/0005-2760(80)90191-5
Links to Exploration step
ISTEX:28E7CF38E2C413AF75BB37C40E8A0CA410535E96Le document en format XML
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Box 1046, Oslo 3, Norway</mods:affiliation></affiliation></author><author><name sortKey="Ose, Turid" sort="Ose, Turid" uniqKey="Ose T" first="Turid" last="Ose">Turid Ose</name><affiliation><mods:affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</mods:affiliation></affiliation></author><author><name sortKey="Norum, Kaare R" sort="Norum, Kaare R" uniqKey="Norum K" first="Kaare R." last="Norum">Kaare R. Norum</name><affiliation><mods:affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</mods:affiliation></affiliation></author><author><name sortKey="Berg, Trond" sort="Berg, Trond" uniqKey="Berg T" first="Trond" last="Berg">Trond Berg</name><affiliation><mods:affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism</title><title level="j" type="abbrev">BBALIP</title><idno type="ISSN">0005-2760</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1980">1980</date><biblScope unit="volume">620</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="120">120</biblScope><biblScope unit="page" to="132">132</biblScope></imprint><idno type="ISSN">0005-2760</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0005-2760</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acid phosphatase</term><term>Acta</term><term>Apolipoprotein</term><term>Beckman ultracentrifuge</term><term>Berg</term><term>Biol</term><term>Biophys</term><term>Bovine serum albumin</term><term>Centrifugation</term><term>Centrifuge tube</term><term>Chloroquine</term><term>Differential centrifugation</term><term>Enzyme activities</term><term>Hepatocytes</term><term>High density lipoproteins</term><term>Homogenate</term><term>Incubation</term><term>Incubation medium</term><term>Intracellular</term><term>Isopycnic</term><term>Isopycnic centrifugation</term><term>Light mitochondrial</term><term>Linear sucrose gradients</term><term>Lipoprotein</term><term>Lysosome</term><term>Major part</term><term>Marker enzymes</term><term>Mitochondrial</term><term>Phosphatase</term><term>Plasma cell membrane</term><term>Postnuclear fractions</term><term>Radioactivity</term><term>Speed centrifugation</term><term>Subcellular</term><term>Subcellular distribution</term><term>Subcellular fractions</term><term>Sucrose</term><term>Sucrose gradients</term><term>Sucrose solution</term><term>Unlabelled</term><term>Vivo</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The subcellular distribution of 125I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. 125I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound 125I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize 125I-labelled HDL. The 125I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of 125I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro 125I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of 125I-labelled HDL taken up by hepatocytes in vitro.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>hepatocytes</json:string><json:string>unlabelled</json:string><json:string>phosphatase</json:string><json:string>subcellular</json:string><json:string>sucrose</json:string><json:string>acid phosphatase</json:string><json:string>lysosome</json:string><json:string>chloroquine</json:string><json:string>lipoprotein</json:string><json:string>incubation medium</json:string><json:string>differential centrifugation</json:string><json:string>acta</json:string><json:string>apolipoprotein</json:string><json:string>subcellular distribution</json:string><json:string>mitochondrial</json:string><json:string>sucrose gradients</json:string><json:string>isopycnic</json:string><json:string>biol</json:string><json:string>intracellular</json:string><json:string>biophys</json:string><json:string>sucrose solution</json:string><json:string>marker enzymes</json:string><json:string>isopycnic centrifugation</json:string><json:string>radioactivity</json:string><json:string>centrifugation</json:string><json:string>speed centrifugation</json:string><json:string>berg</json:string><json:string>light mitochondrial</json:string><json:string>postnuclear fractions</json:string><json:string>bovine serum albumin</json:string><json:string>plasma cell membrane</json:string><json:string>beckman ultracentrifuge</json:string><json:string>linear sucrose gradients</json:string><json:string>enzyme activities</json:string><json:string>centrifuge tube</json:string><json:string>subcellular fractions</json:string><json:string>major part</json:string><json:string>high density lipoproteins</json:string><json:string>homogenate</json:string><json:string>incubation</json:string><json:string>vivo</json:string></teeft></keywords><author><json:item><name>Leiv Ose</name><affiliations><json:string>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</json:string></affiliations></json:item><json:item><name>Turid Ose</name><affiliations><json:string>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</json:string></affiliations></json:item><json:item><name>Kaare R. Norum</name><affiliations><json:string>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</json:string></affiliations></json:item><json:item><name>Trond Berg</name><affiliations><json:string>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>HDL</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Endocytosis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lipoprotein metabolism</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lysosome</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>(Rat hepatocyte)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>VLDL</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>very low density lipoproteins (d > 1.006 g/ml) HDL</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>high density lipoproteins (d = 1.075–1.21 g/ml)</value></json:item></subject><arkIstex>ark:/67375/6H6-RGH25CV5-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The subcellular distribution of 125I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. 125I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound 125I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize 125I-labelled HDL. The 125I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of 125I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro 125I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of 125I-labelled HDL taken up by hepatocytes in vitro.</abstract><qualityIndicators><score>8.827</score><pdfWordCount>4535</pdfWordCount><pdfCharCount>28146</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>13</pdfPageCount><pdfPageSize>468 x 684 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>191</abstractWordCount><abstractCharCount>1259</abstractCharCount><keywordCount>8</keywordCount></qualityIndicators><title>The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes</title><pmid><json:string>6251895</json:string></pmid><pii><json:string>0005-2760(80)90191-5</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism</title><language><json:string>unknown</json:string></language><publicationDate>1980</publicationDate><issn><json:string>0005-2760</json:string></issn><pii><json:string>S0005-2760(00)X0296-2</json:string></pii><volume>620</volume><issue>1</issue><pages><first>120</first><last>132</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>7000</json:string><json:string>12s</json:string><json:string>1980</json:string><json:string>1251</json:string></date><geogName></geogName><orgName><json:string>Andresens Medical Foundation and Anders Jahre Foundation</json:string><json:string>Koch-Light Laboratories</json:string><json:string>University of Oslo</json:string><json:string>Sigma Chemical Co.</json:string><json:string>Hjelpestikkenes Medical Foundation</json:string><json:string>Norwegian Research Council for Science</json:string><json:string>Landsforeningen for Hjerte</json:string><json:string>Norwegian Research Council for Science</json:string><json:string>Humanities</json:string></orgName><orgName_funder><json:string>Landsforeningen for Hjerte</json:string><json:string>Norwegian Research Council for Science</json:string><json:string>Humanities</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Reid</json:string><json:string>El-Aaser</json:string><json:string>In</json:string><json:string>I. 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[19]</json:string><json:string>[24]</json:string><json:string>[19]</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-RGH25CV5-V</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - biochemistry & molecular biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Endocrinology</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biochemistry</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biophysics</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1980</publicationDate><copyrightDate>1980</copyrightDate><doi><json:string>10.1016/0005-2760(80)90191-5</json:string></doi><id>28E7CF38E2C413AF75BB37C40E8A0CA410535E96</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RGH25CV5-V/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RGH25CV5-V/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-RGH25CV5-V/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1980</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes</title><author xml:id="author-0000"><persName><forename type="first">Leiv</forename><surname>Ose</surname></persName><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Turid</forename><surname>Ose</surname></persName><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Kaare R.</forename><surname>Norum</surname></persName><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Trond</forename><surname>Berg</surname></persName><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation></author><idno type="istex">28E7CF38E2C413AF75BB37C40E8A0CA410535E96</idno><idno type="ark">ark:/67375/6H6-RGH25CV5-V</idno><idno type="DOI">10.1016/0005-2760(80)90191-5</idno><idno type="PII">0005-2760(80)90191-5</idno></analytic><monogr><title level="j">Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism</title><title level="j" type="abbrev">BBALIP</title><idno type="pISSN">0005-2760</idno><idno type="PII">S0005-2760(00)X0296-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1980"></date><biblScope unit="volume">620</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="120">120</biblScope><biblScope unit="page" to="132">132</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1980</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The subcellular distribution of 125I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. 125I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound 125I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize 125I-labelled HDL. The 125I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of 125I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro 125I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of 125I-labelled HDL taken up by hepatocytes in vitro.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>HDL</term></item><item><term>Endocytosis</term></item><item><term>Lipoprotein metabolism</term></item><item><term>Lysosome</term></item><item><term>(Rat hepatocyte)</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>VLDL</term></item><item><term>very low density lipoproteins (d < 1.006 g/ml) HDL</term></item><item><term>high density lipoproteins (d = 1.075–1.21 g/ml)</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1980">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RGH25CV5-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>BBALIP</jid><aid>80901915</aid><ce:pii>0005-2760(80)90191-5</ce:pii><ce:doi>10.1016/0005-2760(80)90191-5</ce:doi><ce:copyright type="unknown" year="1980"></ce:copyright></item-info><head><ce:title>The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes</ce:title><ce:author-group><ce:author><ce:given-name>Leiv</ce:given-name><ce:surname>Ose</ce:surname></ce:author><ce:author><ce:given-name>Turid</ce:given-name><ce:surname>Ose</ce:surname></ce:author><ce:author><ce:given-name>Kaare R.</ce:given-name><ce:surname>Norum</ce:surname></ce:author><ce:author><ce:given-name>Trond</ce:given-name><ce:surname>Berg</ce:surname></ce:author><ce:affiliation><ce:textfn>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="29" month="2" year="1980"></ce:date-received><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The subcellular distribution of <ce:sup loc="pre">125</ce:sup>I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. <ce:sup>125</ce:sup>I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound <ce:sup loc="pre">125</ce:sup>I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize <ce:sup loc="pre">125</ce:sup>I-labelled HDL. The <ce:sup loc="pre">125</ce:sup>I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of <ce:sup loc="pre">125</ce:sup>I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro <ce:sup>125</ce:sup>I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of <ce:sup loc="pre">125</ce:sup>I-labelled HDL taken up by hepatocytes in vitro.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>HDL</ce:text></ce:keyword><ce:keyword><ce:text>Endocytosis</ce:text></ce:keyword><ce:keyword><ce:text>Lipoprotein metabolism</ce:text></ce:keyword><ce:keyword><ce:text>Lysosome</ce:text></ce:keyword><ce:keyword><ce:text>(Rat hepatocyte)</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>VLDL</ce:text></ce:keyword><ce:keyword><ce:text>very low density lipoproteins (<ce:italic>d</ce:italic> < 1.006 <ce:italic>g</ce:italic>/<ce:italic>ml</ce:italic>) HDL</ce:text></ce:keyword><ce:keyword><ce:text>high density lipoproteins (<ce:italic>d</ce:italic> = 1.075–1.21 <ce:italic>g</ce:italic>/<ce:italic>ml</ce:italic>)</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes</title></titleInfo><name type="personal"><namePart type="given">Leiv</namePart><namePart type="family">Ose</namePart><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Turid</namePart><namePart type="family">Ose</namePart><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kaare R.</namePart><namePart type="family">Norum</namePart><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Trond</namePart><namePart type="family">Berg</namePart><affiliation>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1980</dateIssued><copyrightDate encoding="w3cdtf">1980</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The subcellular distribution of 125I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. 125I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound 125I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize 125I-labelled HDL. The 125I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of 125I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro 125I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of 125I-labelled HDL taken up by hepatocytes in vitro.</abstract><subject><genre>Keywords</genre><topic>HDL</topic><topic>Endocytosis</topic><topic>Lipoprotein metabolism</topic><topic>Lysosome</topic><topic>(Rat hepatocyte)</topic></subject><subject><genre>Abbreviations</genre><topic>VLDL</topic><topic>very low density lipoproteins (d < 1.006 g/ml) HDL</topic><topic>high density lipoproteins (d = 1.075–1.21 g/ml)</topic></subject><relatedItem type="host"><titleInfo><title>Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism</title></titleInfo><titleInfo type="abbreviated"><title>BBALIP</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1980</dateIssued></originInfo><identifier type="ISSN">0005-2760</identifier><identifier type="PII">S0005-2760(00)X0296-2</identifier><part><date>1980</date><detail type="volume"><number>620</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>176</end></extent><extent unit="pages"><start>120</start><end>132</end></extent></part></relatedItem><identifier type="istex">28E7CF38E2C413AF75BB37C40E8A0CA410535E96</identifier><identifier type="ark">ark:/67375/6H6-RGH25CV5-V</identifier><identifier type="DOI">10.1016/0005-2760(80)90191-5</identifier><identifier type="PII">0005-2760(80)90191-5</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RGH25CV5-V/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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