A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts
Identifieur interne : 000B69 ( Istex/Corpus ); précédent : 000B68; suivant : 000B70A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts
Auteurs : J. Giaimis ; Y. Lombard ; M. Makaya-Kumba ; P. Fonteneau ; P. PoindronSource :
- Journal of Immunological Methods [ 0022-1759 ] ; 1992.
English descriptors
- Teeft :
- Academic press, Acta pathol, Alternative pathway, Autoclaved, Autoclaved yeasts, Biol, Blood clearance, Cell biol, Chloroquine, Culture medium, Cytochalasin, Deep violet color, Escherichia coil, Escherichia coli, Extracellular yeasts, Fetal calf serum, Flow cytometry, Identical experiments, Ingestion, Internalization steps, Intracellular, Intracellular yeasts, Leukocyte biol, Light microscopy, Macrophage, Macrophage membrane, Malu, Malu cells, Malu cells phagocytosing autoclaved yeasts, Mannose, Mannose receptors, Microbial surface, Microorganism, Mononuclear phagocytes, Ofek, Optimal ratio, Phagocytosing cells, Phagocytosis, Phagocytosis test, Pink yeast, Pink yeasts, Receptor, Receptor recycling, Simple method, Staining properties, Surface lectins, Tannic, Tannic acid, Tannic acid solution, Tannic acid treatment, Total number, Total yeasts, Violet yeasts, Yeast.
Abstract
Abstract: Autoclaved yeasts are stained light pink by May-Grünwald Giemsa (MGG). If treated with tannic acid solution just before MGG staining, they display a deep violet color. It seemed possible that these properties could be used to discriminate between extra- and intracellular yeasts in a phagocytosis test, extracellular yeasts being violet and intracellular yeasts being pink. To validate this protocol, quantitative studies of phagocytosis by MALU cells (a murine macrophage cell line) were performed in the presence or absence of drugs known to interfere with phagocytosis.After treatment of cells with cytochalasin B, the mean number of pink yeasts per cell decreased in a dose-dependent manner, the mean number of violet yeasts increased in a dose-dependent manner, whereas the total number of cell-associated yeasts remained almost unchanged whatever the dose used. After treatment with α-mannans or chloroquine, the mean numbers of both violet and pink yeasts decreased in a dose-dependent manner.These results confirmed that (i) violet yeasts are extracellular, (ii) autoclaved yeasts recognize lectin receptors, and (iii) unstained (pink) yeasts are intracellular. We show that this simple method can be used for quantitative light microscopic analysis of both the attachment and internalization steps in the phagocytosis of yeasts.
Url:
DOI: 10.1016/0022-1759(92)90191-U
Links to Exploration step
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Giaimis</name><affiliation><mods:affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</mods:affiliation></affiliation></author><author><name sortKey="Lombard, Y" sort="Lombard, Y" uniqKey="Lombard Y" first="Y." last="Lombard">Y. Lombard</name><affiliation><mods:affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</mods:affiliation></affiliation></author><author><name sortKey="Makaya Kumba, M" sort="Makaya Kumba, M" uniqKey="Makaya Kumba M" first="M." last="Makaya-Kumba">M. Makaya-Kumba</name><affiliation><mods:affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</mods:affiliation></affiliation></author><author><name sortKey="Fonteneau, P" sort="Fonteneau, P" uniqKey="Fonteneau P" first="P." last="Fonteneau">P. 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Poindron</name><affiliation><mods:affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">154</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="185">185</biblScope><biblScope unit="page" to="193">193</biblScope></imprint><idno type="ISSN">0022-1759</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Acta pathol</term><term>Alternative pathway</term><term>Autoclaved</term><term>Autoclaved yeasts</term><term>Biol</term><term>Blood clearance</term><term>Cell biol</term><term>Chloroquine</term><term>Culture medium</term><term>Cytochalasin</term><term>Deep violet color</term><term>Escherichia coil</term><term>Escherichia coli</term><term>Extracellular yeasts</term><term>Fetal calf serum</term><term>Flow cytometry</term><term>Identical experiments</term><term>Ingestion</term><term>Internalization steps</term><term>Intracellular</term><term>Intracellular yeasts</term><term>Leukocyte biol</term><term>Light microscopy</term><term>Macrophage</term><term>Macrophage membrane</term><term>Malu</term><term>Malu cells</term><term>Malu cells phagocytosing autoclaved yeasts</term><term>Mannose</term><term>Mannose receptors</term><term>Microbial surface</term><term>Microorganism</term><term>Mononuclear phagocytes</term><term>Ofek</term><term>Optimal ratio</term><term>Phagocytosing cells</term><term>Phagocytosis</term><term>Phagocytosis test</term><term>Pink yeast</term><term>Pink yeasts</term><term>Receptor</term><term>Receptor recycling</term><term>Simple method</term><term>Staining properties</term><term>Surface lectins</term><term>Tannic</term><term>Tannic acid</term><term>Tannic acid solution</term><term>Tannic acid treatment</term><term>Total number</term><term>Total yeasts</term><term>Violet yeasts</term><term>Yeast</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Autoclaved yeasts are stained light pink by May-Grünwald Giemsa (MGG). If treated with tannic acid solution just before MGG staining, they display a deep violet color. It seemed possible that these properties could be used to discriminate between extra- and intracellular yeasts in a phagocytosis test, extracellular yeasts being violet and intracellular yeasts being pink. To validate this protocol, quantitative studies of phagocytosis by MALU cells (a murine macrophage cell line) were performed in the presence or absence of drugs known to interfere with phagocytosis.After treatment of cells with cytochalasin B, the mean number of pink yeasts per cell decreased in a dose-dependent manner, the mean number of violet yeasts increased in a dose-dependent manner, whereas the total number of cell-associated yeasts remained almost unchanged whatever the dose used. After treatment with α-mannans or chloroquine, the mean numbers of both violet and pink yeasts decreased in a dose-dependent manner.These results confirmed that (i) violet yeasts are extracellular, (ii) autoclaved yeasts recognize lectin receptors, and (iii) unstained (pink) yeasts are intracellular. We show that this simple method can be used for quantitative light microscopic analysis of both the attachment and internalization steps in the phagocytosis of yeasts.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>phagocytosis</json:string><json:string>receptor</json:string><json:string>macrophage</json:string><json:string>tannic</json:string><json:string>cytochalasin</json:string><json:string>autoclaved</json:string><json:string>yeast</json:string><json:string>autoclaved yeasts</json:string><json:string>chloroquine</json:string><json:string>malu</json:string><json:string>tannic acid</json:string><json:string>mannose</json:string><json:string>ofek</json:string><json:string>pink yeasts</json:string><json:string>biol</json:string><json:string>intracellular</json:string><json:string>violet yeasts</json:string><json:string>microorganism</json:string><json:string>mononuclear phagocytes</json:string><json:string>malu cells</json:string><json:string>tannic acid treatment</json:string><json:string>flow cytometry</json:string><json:string>ingestion</json:string><json:string>academic press</json:string><json:string>intracellular yeasts</json:string><json:string>optimal ratio</json:string><json:string>pink yeast</json:string><json:string>mannose receptors</json:string><json:string>deep violet color</json:string><json:string>light microscopy</json:string><json:string>cell biol</json:string><json:string>acta pathol</json:string><json:string>alternative pathway</json:string><json:string>internalization steps</json:string><json:string>culture medium</json:string><json:string>tannic acid solution</json:string><json:string>malu cells phagocytosing autoclaved yeasts</json:string><json:string>fetal calf serum</json:string><json:string>simple method</json:string><json:string>identical experiments</json:string><json:string>microbial surface</json:string><json:string>total yeasts</json:string><json:string>staining properties</json:string><json:string>total number</json:string><json:string>phagocytosis test</json:string><json:string>surface lectins</json:string><json:string>macrophage membrane</json:string><json:string>escherichia coli</json:string><json:string>extracellular yeasts</json:string><json:string>phagocytosing cells</json:string><json:string>leukocyte biol</json:string><json:string>blood clearance</json:string><json:string>escherichia coil</json:string><json:string>receptor recycling</json:string></teeft></keywords><author><json:item><name>J. Giaimis</name><affiliations><json:string>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</json:string></affiliations></json:item><json:item><name>Y. Lombard</name><affiliations><json:string>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</json:string></affiliations></json:item><json:item><name>M. Makaya-Kumba</name><affiliations><json:string>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</json:string></affiliations></json:item><json:item><name>P. Fonteneau</name><affiliations><json:string>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</json:string></affiliations></json:item><json:item><name>P. Poindron</name><affiliations><json:string>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Phagocytosis (quantitative study of)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Yeast</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>α-Mannan</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cytochalasin B</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Chloroquine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CAY : cell-associated yeasts</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>FCS : fetal calf serum</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>MGG : May-Grünwald Giemsa</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>VY : violet yeasts</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PY : pink yeasts</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Y/C : yeast to cell ratio</value></json:item></subject><arkIstex>ark:/67375/6H6-KH1BHKBW-B</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Autoclaved yeasts are stained light pink by May-Grünwald Giemsa (MGG). If treated with tannic acid solution just before MGG staining, they display a deep violet color. It seemed possible that these properties could be used to discriminate between extra- and intracellular yeasts in a phagocytosis test, extracellular yeasts being violet and intracellular yeasts being pink. To validate this protocol, quantitative studies of phagocytosis by MALU cells (a murine macrophage cell line) were performed in the presence or absence of drugs known to interfere with phagocytosis.After treatment of cells with cytochalasin B, the mean number of pink yeasts per cell decreased in a dose-dependent manner, the mean number of violet yeasts increased in a dose-dependent manner, whereas the total number of cell-associated yeasts remained almost unchanged whatever the dose used. After treatment with α-mannans or chloroquine, the mean numbers of both violet and pink yeasts decreased in a dose-dependent manner.These results confirmed that (i) violet yeasts are extracellular, (ii) autoclaved yeasts recognize lectin receptors, and (iii) unstained (pink) yeasts are intracellular. We show that this simple method can be used for quantitative light microscopic analysis of both the attachment and internalization steps in the phagocytosis of yeasts.</abstract><qualityIndicators><score>8.511</score><pdfWordCount>4147</pdfWordCount><pdfCharCount>22706</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>360 x 468 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>197</abstractWordCount><abstractCharCount>1347</abstractCharCount><keywordCount>11</keywordCount></qualityIndicators><title>A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts</title><pmid><json:string>1383342</json:string></pmid><pii><json:string>0022-1759(92)90191-U</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. USA</title><language><json:string>unknown</json:string></language><volume>69</volume><pages><first>442</first></pages></serie><host><title>Journal of Immunological Methods</title><language><json:string>unknown</json:string></language><publicationDate>1992</publicationDate><issn><json:string>0022-1759</json:string></issn><pii><json:string>S0022-1759(00)X0534-0</json:string></pii><volume>154</volume><issue>2</issue><pages><first>185</first><last>193</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1992</json:string></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>C.D. Muller</json:string><json:string>E. Note</json:string><json:string>S. Braun</json:string><json:string>Louis Pasteur</json:string><json:string>Professor Pesson</json:string><json:string>K. Takeda</json:string><json:string>P. Poindron</json:string></persName><placeName><json:string>PY</json:string><json:string>Germany</json:string><json:string>Darmstadt</json:string><json:string>Strasbourg</json:string><json:string>France</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Axline and Reaven, 1974</json:string><json:string>Ogmundsdottir ano Weir, 1976</json:string><json:string>Silverstein et al., 1977</json:string><json:string>Rollag, 1979</json:string><json:string>Perry et al., 1985</json:string><json:string>Lombard et al. (1988)</json:string><json:string>Gonzales-Noriega et al., 1980</json:string><json:string>Lombard et al., 1985</json:string><json:string>Freimer et al., 1978</json:string><json:string>Stahl et al., 1980</json:string><json:string>Verhoef and Waldvogel, 1985</json:string><json:string>Tietze et al., 1980</json:string><json:string>Mantovani et al., 1972</json:string><json:string>Bassoe and Solberg, 1984</json:string><json:string>Brown, 1991</json:string><json:string>Perry and Ofek, 1984</json:string><json:string>Muller et al., 1988</json:string><json:string>Sharon, 1984</json:string><json:string>Fearon and Austen, 1980</json:string><json:string>Spudich and Lin, 1972</json:string><json:string>Lehrer, 1981</json:string><json:string>Lombard et al., 1988</json:string><json:string>Bassoe et al., 1980</json:string><json:string>Sung et al., 1983</json:string><json:string>Bianco et al., 1975</json:string><json:string>Stahl, 1990</json:string><json:string>Shaw and Griffin, 1981</json:string><json:string>Stephenson and Shepherd, 1987</json:string><json:string>Verhoef et al., 1977</json:string><json:string>Ofek and Sharon, 1988</json:string><json:string>Gordon and Mokoena, 1989</json:string><json:string>Wilson et al., 1985</json:string><json:string>Shavit et al., 1980</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-KH1BHKBW-B</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - immunology</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - immunology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Immunology and Allergy</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - vertebres: systeme digestif</json:string></inist></categories><publicationDate>1992</publicationDate><copyrightDate>1992</copyrightDate><doi><json:string>10.1016/0022-1759(92)90191-U</json:string></doi><id>10782CCF9948DE85622A8068D3A12F86A010BB0C</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KH1BHKBW-B/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KH1BHKBW-B/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-KH1BHKBW-B/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1992</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts</title><author xml:id="author-0000"><persName><forename type="first">J.</forename><surname>Giaimis</surname></persName><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Y.</forename><surname>Lombard</surname></persName><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">M.</forename><surname>Makaya-Kumba</surname></persName><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">P.</forename><surname>Fonteneau</surname></persName><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">P.</forename><surname>Poindron</surname></persName><note type="correspondence"><p>Correspondence to: P. Poindron, Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, BP 24, F-67401 Illkirch Cedex, France. Tel.: 88 67 69 27; Fax: 88 66 01 90.</p></note><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation></author><idno type="istex">10782CCF9948DE85622A8068D3A12F86A010BB0C</idno><idno type="ark">ark:/67375/6H6-KH1BHKBW-B</idno><idno type="DOI">10.1016/0022-1759(92)90191-U</idno><idno type="PII">0022-1759(92)90191-U</idno></analytic><monogr><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="pISSN">0022-1759</idno><idno type="PII">S0022-1759(00)X0534-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992"></date><biblScope unit="volume">154</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="185">185</biblScope><biblScope unit="page" to="193">193</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1992</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Autoclaved yeasts are stained light pink by May-Grünwald Giemsa (MGG). If treated with tannic acid solution just before MGG staining, they display a deep violet color. It seemed possible that these properties could be used to discriminate between extra- and intracellular yeasts in a phagocytosis test, extracellular yeasts being violet and intracellular yeasts being pink. To validate this protocol, quantitative studies of phagocytosis by MALU cells (a murine macrophage cell line) were performed in the presence or absence of drugs known to interfere with phagocytosis.After treatment of cells with cytochalasin B, the mean number of pink yeasts per cell decreased in a dose-dependent manner, the mean number of violet yeasts increased in a dose-dependent manner, whereas the total number of cell-associated yeasts remained almost unchanged whatever the dose used. After treatment with α-mannans or chloroquine, the mean numbers of both violet and pink yeasts decreased in a dose-dependent manner.These results confirmed that (i) violet yeasts are extracellular, (ii) autoclaved yeasts recognize lectin receptors, and (iii) unstained (pink) yeasts are intracellular. We show that this simple method can be used for quantitative light microscopic analysis of both the attachment and internalization steps in the phagocytosis of yeasts.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Phagocytosis (quantitative study of)</term></item><item><term>Yeast</term></item><item><term>α-Mannan</term></item><item><term>Cytochalasin B</term></item><item><term>Chloroquine</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>CAY</term><term>cell-associated yeasts</term></item><item><term>FCS</term><term>fetal calf serum</term></item><item><term>MGG</term><term>May-Grünwald Giemsa</term></item><item><term>VY</term><term>violet yeasts</term></item><item><term>PY</term><term>pink yeasts</term></item><item><term>Y/C</term><term>yeast to cell ratio</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1992-03-04">Modified</change><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KH1BHKBW-B/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" </istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JIM</jid><aid>90191</aid><ce:pii>0022-1759(92)90191-U</ce:pii><ce:doi>10.1016/0022-1759(92)90191-U</ce:doi><ce:copyright type="unknown" year="1992"></ce:copyright></item-info><head><ce:title>A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts</ce:title><ce:author-group><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Giaimis</ce:surname></ce:author><ce:author><ce:given-name>Y.</ce:given-name><ce:surname>Lombard</ce:surname></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Makaya-Kumba</ce:surname></ce:author><ce:author><ce:given-name>P.</ce:given-name><ce:surname>Fonteneau</ce:surname></ce:author><ce:author><ce:given-name>P.</ce:given-name><ce:surname>Poindron</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: P. Poindron, Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, BP 24, F-67401 Illkirch Cedex, France. Tel.: 88 67 69 27; Fax: 88 66 01 90.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="23" month="10" year="1991"></ce:date-received><ce:date-revised day="4" month="3" year="1992"></ce:date-revised><ce:date-accepted day="30" month="4" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para id="SP0005">Autoclaved yeasts are stained light pink by May-Grünwald Giemsa (MGG). If treated with tannic acid solution just before MGG staining, they display a deep violet color. It seemed possible that these properties could be used to discriminate between extra- and intracellular yeasts in a phagocytosis test, extracellular yeasts being violet and intracellular yeasts being pink. To validate this protocol, quantitative studies of phagocytosis by MALU cells (a murine macrophage cell line) were performed in the presence or absence of drugs known to interfere with phagocytosis.</ce:simple-para><ce:simple-para id="SP0010">After treatment of cells with cytochalasin B, the mean number of pink yeasts per cell decreased in a dose-dependent manner, the mean number of violet yeasts increased in a dose-dependent manner, whereas the total number of cell-associated yeasts remained almost unchanged whatever the dose used. After treatment with α-mannans or chloroquine, the mean numbers of both violet and pink yeasts decreased in a dose-dependent manner.</ce:simple-para><ce:simple-para id="SP0015">These results confirmed that (i) violet yeasts are extracellular, (ii) autoclaved yeasts recognize lectin receptors, and (iii) unstained (pink) yeasts are intracellular. We show that this simple method can be used for quantitative light microscopic analysis of both the attachment and internalization steps in the phagocytosis of yeasts.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Phagocytosis (quantitative study of)</ce:text></ce:keyword><ce:keyword><ce:text>Yeast</ce:text></ce:keyword><ce:keyword><ce:text>α-Mannan</ce:text></ce:keyword><ce:keyword><ce:text>Cytochalasin B</ce:text></ce:keyword><ce:keyword><ce:text>Chloroquine</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>CAY</ce:text><ce:keyword><ce:text>cell-associated yeasts</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>FCS</ce:text><ce:keyword><ce:text>fetal calf serum</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>MGG</ce:text><ce:keyword><ce:text>May-Grünwald Giemsa</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>VY</ce:text><ce:keyword><ce:text>violet yeasts</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PY</ce:text><ce:keyword><ce:text>pink yeasts</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Y/C</ce:text><ce:keyword><ce:text>yeast to cell ratio</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Giaimis</namePart><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Lombard</namePart><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Makaya-Kumba</namePart><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Fonteneau</namePart><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Poindron</namePart><affiliation>Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, Illkirch, France</affiliation><description>Correspondence to: P. Poindron, Université Louis Pasteur, Centre de Recherches Pharmaceutiques, Département d'Immunologie et Immunopharmacologie, BP 24, F-67401 Illkirch Cedex, France. Tel.: 88 67 69 27; Fax: 88 66 01 90.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><dateModified encoding="w3cdtf">1992-03-04</dateModified><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Autoclaved yeasts are stained light pink by May-Grünwald Giemsa (MGG). If treated with tannic acid solution just before MGG staining, they display a deep violet color. It seemed possible that these properties could be used to discriminate between extra- and intracellular yeasts in a phagocytosis test, extracellular yeasts being violet and intracellular yeasts being pink. To validate this protocol, quantitative studies of phagocytosis by MALU cells (a murine macrophage cell line) were performed in the presence or absence of drugs known to interfere with phagocytosis.After treatment of cells with cytochalasin B, the mean number of pink yeasts per cell decreased in a dose-dependent manner, the mean number of violet yeasts increased in a dose-dependent manner, whereas the total number of cell-associated yeasts remained almost unchanged whatever the dose used. After treatment with α-mannans or chloroquine, the mean numbers of both violet and pink yeasts decreased in a dose-dependent manner.These results confirmed that (i) violet yeasts are extracellular, (ii) autoclaved yeasts recognize lectin receptors, and (iii) unstained (pink) yeasts are intracellular. We show that this simple method can be used for quantitative light microscopic analysis of both the attachment and internalization steps in the phagocytosis of yeasts.</abstract><subject><genre>Keywords</genre><topic>Phagocytosis (quantitative study of)</topic><topic>Yeast</topic><topic>α-Mannan</topic><topic>Cytochalasin B</topic><topic>Chloroquine</topic></subject><subject><genre>Abbreviations</genre><topic>CAY : cell-associated yeasts</topic><topic>FCS : fetal calf serum</topic><topic>MGG : May-Grünwald Giemsa</topic><topic>VY : violet yeasts</topic><topic>PY : pink yeasts</topic><topic>Y/C : yeast to cell ratio</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Immunological Methods</title></titleInfo><titleInfo type="abbreviated"><title>JIM</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0022-1759</identifier><identifier type="PII">S0022-1759(00)X0534-0</identifier><part><date>1992</date><detail type="volume"><number>154</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>147</start><end>278</end></extent><extent unit="pages"><start>185</start><end>193</end></extent></part></relatedItem><identifier type="istex">10782CCF9948DE85622A8068D3A12F86A010BB0C</identifier><identifier type="ark">ark:/67375/6H6-KH1BHKBW-B</identifier><identifier type="DOI">10.1016/0022-1759(92)90191-U</identifier><identifier type="PII">0022-1759(92)90191-U</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KH1BHKBW-B/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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