Differential Ability of Human Endothelial Cells to Internalize and Express Exogenous DNA
Identifieur interne : 000600 ( Istex/Corpus ); précédent : 000599; suivant : 000601Differential Ability of Human Endothelial Cells to Internalize and Express Exogenous DNA
Auteurs : Maria Giovanna Colombo ; Lorenzo Citti ; Giuseppina Basta ; Raffaele De Caterina ; Andrea Biagini ; Giuseppe RainaldiSource :
- Cardiovascular Drugs and Therapy [ 0920-3206 ] ; 2001-01-01.
English descriptors
Abstract
Abstract: Vascular endothelium gene expression regulates blood-vessel wall interactions, vascular permeability, smooth muscle cell growth and tone. The possibility to introduce exogenous DNA or RNA sequences in endothelial cells represents a novel therapeutic approach of vascular disease. The aim of the work was to investigate the ability of endothelial cells to internalize and express exogenous DNA sequences. Human umbilical vein endothelial cells (HUVEC) were transfected with either a 780 bp fluorescein-labeled DNA (FITC-DNA) or pEGFP-C1 plasmid encoding for a green fluorescent protein (GFP), using the cationic liposome DOTAP as transfection reagent. The transfected cell population was passed through a FACScan apparatus and percentage of fluorescent cells was determined using a FACScan analysis programme. The SW620 tumor-derived cell line was used as control. The percentage of FITC-DNA positive cells was 66.0% for HUVEC and 45.0% for SW620 cells. On the contrary, the percentage of GFP-positive cells was 13.8% and 43% for HUVEC and SW620, respectively. By increasing the amount of DNA as well as the protocol of administration the percentage of GFP-positive HUVEC was enhanced suggesting a rapid degradation of DNA in the HUVEC cytoplasm.
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DOI: 10.1023/A:1011154616992
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ISTEX:8DE5A7DF28102455A9E5E3313511F2C7095A41B5Le document en format XML
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Andrea" sort="Biagini, Andrea" uniqKey="Biagini A" first="Andrea" last="Biagini">Andrea Biagini</name><affiliation><mods:affiliation>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</mods:affiliation></affiliation></author><author><name sortKey="Rainaldi, Giuseppe" sort="Rainaldi, Giuseppe" uniqKey="Rainaldi G" first="Giuseppe" last="Rainaldi">Giuseppe Rainaldi</name><affiliation><mods:affiliation>Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: g.rainaldi@imd.pi.cnr.it</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cardiovascular Drugs and Therapy</title><title level="j" type="abbrev">Cardiovasc Drugs Ther</title><idno type="ISSN">0920-3206</idno><idno type="eISSN">1573-7241</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><date type="published" when="2001-01-01">2001-01-01</date><biblScope 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The possibility to introduce exogenous DNA or RNA sequences in endothelial cells represents a novel therapeutic approach of vascular disease. The aim of the work was to investigate the ability of endothelial cells to internalize and express exogenous DNA sequences. Human umbilical vein endothelial cells (HUVEC) were transfected with either a 780 bp fluorescein-labeled DNA (FITC-DNA) or pEGFP-C1 plasmid encoding for a green fluorescent protein (GFP), using the cationic liposome DOTAP as transfection reagent. The transfected cell population was passed through a FACScan apparatus and percentage of fluorescent cells was determined using a FACScan analysis programme. The SW620 tumor-derived cell line was used as control. The percentage of FITC-DNA positive cells was 66.0% for HUVEC and 45.0% for SW620 cells. On the contrary, the percentage of GFP-positive cells was 13.8% and 43% for HUVEC and SW620, respectively. By increasing the amount of DNA as well as the protocol of administration the percentage of GFP-positive HUVEC was enhanced suggesting a rapid degradation of DNA in the HUVEC cytoplasm.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Maria Giovanna Colombo</name><affiliations><json:string>Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy</json:string><json:string>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</json:string></affiliations></json:item><json:item><name>Lorenzo Citti</name><affiliations><json:string>Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy</json:string></affiliations></json:item><json:item><name>Giuseppina Basta</name><affiliations><json:string>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</json:string></affiliations></json:item><json:item><name>Raffaele De Caterina</name><affiliations><json:string>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</json:string></affiliations></json:item><json:item><name>Andrea Biagini</name><affiliations><json:string>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</json:string></affiliations></json:item><json:item><name>Giuseppe Rainaldi</name><affiliations><json:string>Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy</json:string><json:string>E-mail: g.rainaldi@imd.pi.cnr.it</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>human endothelial cells</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pharmacology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>gene delivery</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>gene expression</value></json:item></subject><articleId><json:string>336296</json:string><json:string>Art6</json:string></articleId><arkIstex>ark:/67375/VQC-91SH4C0X-M</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Vascular endothelium gene expression regulates blood-vessel wall interactions, vascular permeability, smooth muscle cell growth and tone. The possibility to introduce exogenous DNA or RNA sequences in endothelial cells represents a novel therapeutic approach of vascular disease. The aim of the work was to investigate the ability of endothelial cells to internalize and express exogenous DNA sequences. Human umbilical vein endothelial cells (HUVEC) were transfected with either a 780 bp fluorescein-labeled DNA (FITC-DNA) or pEGFP-C1 plasmid encoding for a green fluorescent protein (GFP), using the cationic liposome DOTAP as transfection reagent. The transfected cell population was passed through a FACScan apparatus and percentage of fluorescent cells was determined using a FACScan analysis programme. The SW620 tumor-derived cell line was used as control. The percentage of FITC-DNA positive cells was 66.0% for HUVEC and 45.0% for SW620 cells. On the contrary, the percentage of GFP-positive cells was 13.8% and 43% for HUVEC and SW620, respectively. 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The possibility to introduce exogenous DNA or RNA sequences in endothelial cells represents a novel therapeutic approach of vascular disease. The aim of the work was to investigate the ability of endothelial cells to internalize and express exogenous DNA sequences. Human umbilical vein endothelial cells (HUVEC) were transfected with either a 780 bp fluorescein-labeled DNA (FITC-DNA) or pEGFP-C1 plasmid encoding for a green fluorescent protein (GFP), using the cationic liposome DOTAP as transfection reagent. The transfected cell population was passed through a FACScan apparatus and percentage of fluorescent cells was determined using a FACScan analysis programme. The SW620 tumor-derived cell line was used as control. The percentage of FITC-DNA positive cells was 66.0% for HUVEC and 45.0% for SW620 cells. On the contrary, the percentage of GFP-positive cells was 13.8% and 43% for HUVEC and SW620, respectively. By increasing the amount of DNA as well as the protocol of administration the percentage of GFP-positive HUVEC was enhanced suggesting a rapid degradation of DNA in the HUVEC cytoplasm.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>human endothelial cells</term></item><item><term>pharmacology</term></item><item><term>gene delivery</term></item><item><term>gene expression</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Medicine & Public Health</head><item><term>Cardiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001-01-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-91SH4C0X-M/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" 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DNA</ArticleTitle><ArticleFirstPage>25</ArticleFirstPage><ArticleLastPage>29</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>10</Month><Day>19</Day></RegistrationDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>2001</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>10557</JournalID><VolumeIDStart>15</VolumeIDStart><VolumeIDEnd>15</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Maria</GivenName><GivenName>Giovanna</GivenName><FamilyName>Colombo</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Lorenzo</GivenName><FamilyName>Citti</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Giuseppina</GivenName><FamilyName>Basta</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Raffaele</GivenName><FamilyName>De Caterina</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Andrea</GivenName><FamilyName>Biagini</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Giuseppe</GivenName><FamilyName>Rainaldi</FamilyName></AuthorName><Contact><Email>g.rainaldi@imd.pi.cnr.it</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Istituto di Mutagenesi e Differenziamento</OrgDivision><OrgName>CNR</OrgName><OrgAddress><City>Pisa</City><Country>Italy</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Istituto di Fisiologia Clinica</OrgDivision><OrgName>CNR</OrgName><OrgAddress><City>Pisa</City><Country>Italy</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Vascular endothelium gene expression regulates blood-vessel wall interactions, vascular permeability, smooth muscle cell growth and tone. The possibility to introduce exogenous DNA or RNA sequences in endothelial cells represents a novel therapeutic approach of vascular disease. The aim of the work was to investigate the ability of endothelial cells to internalize and express exogenous DNA sequences. Human umbilical vein endothelial cells (HUVEC) were transfected with either a 780 bp fluorescein-labeled DNA (FITC-DNA) or pEGFP-C1 plasmid encoding for a green fluorescent protein (GFP), using the cationic liposome DOTAP as transfection reagent. The transfected cell population was passed through a FACScan apparatus and percentage of fluorescent cells was determined using a FACScan analysis programme. The SW620 tumor-derived cell line was used as control. The percentage of FITC-DNA positive cells was 66.0% for HUVEC and 45.0% for SW620 cells. On the contrary, the percentage of GFP-positive cells was 13.8% and 43% for HUVEC and SW620, respectively. By increasing the amount of DNA as well as the protocol of administration the percentage of GFP-positive HUVEC was enhanced suggesting a rapid degradation of DNA in the HUVEC cytoplasm.</Para></Abstract><KeywordGroup Language="En"><Keyword>human endothelial cells</Keyword><Keyword>pharmacology</Keyword><Keyword>gene delivery</Keyword><Keyword>gene expression</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Differential Ability of Human Endothelial Cells to Internalize and Express Exogenous DNA</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Differential Ability of Human Endothelial Cells to Internalize and Express Exogenous DNA</title></titleInfo><name type="personal"><namePart type="given">Maria</namePart><namePart type="given">Giovanna</namePart><namePart type="family">Colombo</namePart><affiliation>Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy</affiliation><affiliation>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lorenzo</namePart><namePart type="family">Citti</namePart><affiliation>Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Giuseppina</namePart><namePart type="family">Basta</namePart><affiliation>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Raffaele</namePart><namePart type="family">De Caterina</namePart><affiliation>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Andrea</namePart><namePart type="family">Biagini</namePart><affiliation>Istituto di Fisiologia Clinica, CNR, Pisa, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Giuseppe</namePart><namePart type="family">Rainaldi</namePart><affiliation>Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy</affiliation><affiliation>E-mail: g.rainaldi@imd.pi.cnr.it</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Boston</placeTerm></place><dateIssued encoding="w3cdtf">2001-01-01</dateIssued><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Vascular endothelium gene expression regulates blood-vessel wall interactions, vascular permeability, smooth muscle cell growth and tone. The possibility to introduce exogenous DNA or RNA sequences in endothelial cells represents a novel therapeutic approach of vascular disease. The aim of the work was to investigate the ability of endothelial cells to internalize and express exogenous DNA sequences. Human umbilical vein endothelial cells (HUVEC) were transfected with either a 780 bp fluorescein-labeled DNA (FITC-DNA) or pEGFP-C1 plasmid encoding for a green fluorescent protein (GFP), using the cationic liposome DOTAP as transfection reagent. The transfected cell population was passed through a FACScan apparatus and percentage of fluorescent cells was determined using a FACScan analysis programme. The SW620 tumor-derived cell line was used as control. The percentage of FITC-DNA positive cells was 66.0% for HUVEC and 45.0% for SW620 cells. On the contrary, the percentage of GFP-positive cells was 13.8% and 43% for HUVEC and SW620, respectively. By increasing the amount of DNA as well as the protocol of administration the percentage of GFP-positive HUVEC was enhanced suggesting a rapid degradation of DNA in the HUVEC cytoplasm.</abstract><subject lang="en"><topic>human endothelial cells</topic><topic>pharmacology</topic><topic>gene delivery</topic><topic>gene expression</topic></subject><relatedItem type="host"><titleInfo><title>Cardiovascular Drugs and Therapy</title></titleInfo><titleInfo type="abbreviated"><title>Cardiovasc Drugs Ther</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2001-01-01</dateIssued><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><subject><genre>Medicine & Public Health</genre><topic>Cardiology</topic></subject><identifier type="ISSN">0920-3206</identifier><identifier type="eISSN">1573-7241</identifier><identifier type="JournalID">10557</identifier><identifier type="IssueArticleCount">15</identifier><identifier type="VolumeIssueCount">7</identifier><part><date>2001</date><detail type="volume"><number>15</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>25</start><end>29</end></extent></part><recordInfo><recordOrigin>Kluwer Academic Publishers, 2001</recordOrigin></recordInfo></relatedItem><identifier type="istex">8DE5A7DF28102455A9E5E3313511F2C7095A41B5</identifier><identifier type="ark">ark:/67375/VQC-91SH4C0X-M</identifier><identifier type="DOI">10.1023/A:1011154616992</identifier><identifier type="ArticleID">336296</identifier><identifier type="ArticleID">Art6</identifier><accessCondition type="use and reproduction" contentType="copyright">Kluwer Academic Publishers, 2001</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Kluwer Academic Publishers, 2001</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-91SH4C0X-M/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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