Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells
Identifieur interne : 000219 ( Hal/Corpus ); précédent : 000218; suivant : 000220Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells
Auteurs : Patrick Midoux ; Christina Mendes ; Alain Legrand ; Jacques Raimond ; Roger Mayer ; Michel Monsigny ; Annie Claude RocheSource :
- Nucleic Acids Research [ 0305-1048 ] ; 1993-02-25.
Abstract
Plasmid DNA/glycosylated polylysine complexes were used to transfer in vitro a luciferase reporter gene into human hepatoma cells by a receptor-mediated endocytosis process. HepG2 cells which express a galactose specific membrane lectin were efficiently and selectively transfected with pSV2Luc/lactosylated polylysine complexes in a sugar dependent manner: i) HepG2 cells which do not express membrane lectin specific for mannose were quite poorly transfected with pSV2Luc/mannosylated polylysine complexes, ii) HeLa cells which do not express membrane lectin specific for galactose were not transfected with pSV2Luc/lactosylated polylysine complexes. The transfection efficiency of HepG2 cells with pSV2Luc/lactosylated polylysine complexes was greatly enhanced either in the presence of chloroquine or in the presence of a fusogenic peptide. A 22-residue peptide derived from the influenza virus hemagglutinin HA2 N-terminal polypeptide that mimics the fusogenic activity of the virus, was selected. In the presence of the fusogenic peptide, the luciferase activity in HepG2 cells was 10 fold larger than that of cells transfected with pSV2Luc/lactosylated polylysine complexes in the presence of chloroquine.
Url:
DOI: 10.1093/nar/21.4.871
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Hal:hal-02138456Le document en format XML
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Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Roche, Annie Claude" sort="Roche, Annie Claude" uniqKey="Roche A" first="Annie Claude" last="Roche">Annie Claude Roche</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno> <idno type="RNSR">196717612S</idno> <orgName>Centre de biophysique moléculaire</orgName> <orgName type="acronym">CBM</orgName> <date type="start">1967-01-01</date> <desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://cbm.cnrs-orleans.fr/</ref> </desc> <listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation> <relation name="UPR4301" active="#struct-303623" type="direct"></relation> <relation name="UPR4301" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno> <idno type="ISNI">0000000121581666 </idno> <orgName>Université d'Orléans</orgName> <orgName type="acronym">UO</orgName> <desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author></analytic><idno type="DOI">10.1093/nar/21.4.871</idno><series><title level="j">Nucleic Acids Research</title><idno type="ISSN">0305-1048</idno><imprint><date type="datePub">1993-02-25</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Plasmid DNA/glycosylated polylysine complexes were used to transfer in vitro a luciferase reporter gene into human hepatoma cells by a receptor-mediated endocytosis process. HepG2 cells which express a galactose specific membrane lectin were efficiently and selectively transfected with pSV2Luc/lactosylated polylysine complexes in a sugar dependent manner: i) HepG2 cells which do not express membrane lectin specific for mannose were quite poorly transfected with pSV2Luc/mannosylated polylysine complexes, ii) HeLa cells which do not express membrane lectin specific for galactose were not transfected with pSV2Luc/lactosylated polylysine complexes. The transfection efficiency of HepG2 cells with pSV2Luc/lactosylated polylysine complexes was greatly enhanced either in the presence of chloroquine or in the presence of a fusogenic peptide. A 22-residue peptide derived from the influenza virus hemagglutinin HA2 N-terminal polypeptide that mimics the fusogenic activity of the virus, was selected. In the presence of the fusogenic peptide, the luciferase activity in HepG2 cells was 10 fold larger than that of cells transfected with pSV2Luc/lactosylated polylysine complexes in the presence of chloroquine.</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells</title> <author role="aut"> <persName> <forename type="first">Patrick</forename> <surname>Midoux</surname> </persName> <email type="md5">c8f24a3cf955469aa969463cc1db0798</email> <email type="domain">cnrs-orleans.fr</email> <idno type="idhal" notation="string">patrick-midoux</idno> <idno type="idhal" notation="numeric">176062</idno> <idno type="halauthorid">67616</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Christina</forename> <surname>Mendes</surname> </persName> <idno type="halauthorid">11494855</idno> </author> <author role="aut"> <persName> <forename type="first">Alain</forename> <surname>Legrand</surname> </persName> <email type="md5">2f12ba3c198c214fe4df328a9d393187</email> <email type="domain">cnrs-orleans.fr</email> <idno type="idhal" notation="string">alain-legrand</idno> <idno type="idhal" notation="numeric">175092</idno> <idno type="halauthorid">533342</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jacques</forename> <surname>Raimond</surname> </persName> <idno type="halauthorid">11494435</idno> </author> <author role="aut"> <persName> <forename type="first">Roger</forename> <surname>Mayer</surname> </persName> <idno type="halauthorid">11470491</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Michel</forename> <surname>Monsigny</surname> </persName> <idno type="halauthorid">200710</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Annie Claude</forename> <surname>Roche</surname> </persName> <idno type="halauthorid">11494856</idno> <affiliation ref="#struct-451"></affiliation> </author> <editor role="depositor"> <persName> <forename>Laëtitia</forename> <surname>LEGOUPIL</surname> </persName> <email type="md5">e9a3fa0bdfd22a9d0a2d2ac46e7772a2</email> <email type="domain">cnrs-orleans.fr</email> </editor> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2019-05-23 17:53:48</date> <date type="whenModified">2019-06-20 12:22:09</date> <date type="whenReleased">2019-05-23 17:53:48</date> <date type="whenProduced">1993-02-25</date> <ref type="externalLink" target="http://europepmc.org/articles/pmc309219?pdf=render"></ref> </edition> <respStmt> <resp>contributor</resp> <name key="712555"> <persName> <forename>Laëtitia</forename> <surname>LEGOUPIL</surname> </persName> <email type="md5">e9a3fa0bdfd22a9d0a2d2ac46e7772a2</email> <email type="domain">cnrs-orleans.fr</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">hal-02138456</idno> <idno type="halUri">https://hal.archives-ouvertes.fr/hal-02138456</idno> <idno type="halBibtex">midoux:hal-02138456</idno> <idno type="halRefHtml">Nucleic Acids Research, Oxford University Press, 1993, 21 (4), pp.871-878. ⟨10.1093/nar/21.4.871⟩</idno> <idno type="halRef">Nucleic Acids Research, Oxford University Press, 1993, 21 (4), pp.871-878. ⟨10.1093/nar/21.4.871⟩</idno> </publicationStmt> <seriesStmt> <idno type="stamp" n="CBM">Centre de biophysique moléculaire</idno> <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno> <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno> <idno type="stamp" n="UNIV-ORLEANS">Université d'Orléans</idno> </seriesStmt> <notesStmt> <note type="audience" n="2">International</note> <note type="popular" n="0">No</note> <note type="peer" n="1">Yes</note> </notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells</title> <author role="aut"> <persName> <forename type="first">Patrick</forename> <surname>Midoux</surname> </persName> <email type="md5">c8f24a3cf955469aa969463cc1db0798</email> <email type="domain">cnrs-orleans.fr</email> <idno type="idhal" notation="string">patrick-midoux</idno> <idno type="idhal" notation="numeric">176062</idno> <idno type="halauthorid">67616</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Christina</forename> <surname>Mendes</surname> </persName> <idno type="halauthorid">11494855</idno> </author> <author role="aut"> <persName> <forename type="first">Alain</forename> <surname>Legrand</surname> </persName> <email type="md5">2f12ba3c198c214fe4df328a9d393187</email> <email type="domain">cnrs-orleans.fr</email> <idno type="idhal" notation="string">alain-legrand</idno> <idno type="idhal" notation="numeric">175092</idno> <idno type="halauthorid">533342</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jacques</forename> <surname>Raimond</surname> </persName> <idno type="halauthorid">11494435</idno> </author> <author role="aut"> <persName> <forename type="first">Roger</forename> <surname>Mayer</surname> </persName> <idno type="halauthorid">11470491</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Michel</forename> <surname>Monsigny</surname> </persName> <idno type="halauthorid">200710</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Annie Claude</forename> <surname>Roche</surname> </persName> <idno type="halauthorid">11494856</idno> <affiliation ref="#struct-451"></affiliation> </author> </analytic> <monogr> <idno type="halJournalId" status="VALID">1850</idno> <idno type="issn">0305-1048</idno> <idno type="eissn">1362-4962</idno> <title level="j">Nucleic Acids Research</title> <imprint> <publisher>Oxford University Press</publisher> <biblScope unit="volume">21</biblScope> <biblScope unit="issue">4</biblScope> <biblScope unit="pp">871-878</biblScope> <date type="datePub">1993-02-25</date> </imprint> </monogr> <idno type="doi">10.1093/nar/21.4.871</idno> <idno type="pubmed">8383843</idno> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode> <classCode scheme="halTypology" n="ART">Journal articles</classCode> </textClass> <abstract xml:lang="en"> <p>Plasmid DNA/glycosylated polylysine complexes were used to transfer in vitro a luciferase reporter gene into human hepatoma cells by a receptor-mediated endocytosis process. HepG2 cells which express a galactose specific membrane lectin were efficiently and selectively transfected with pSV2Luc/lactosylated polylysine complexes in a sugar dependent manner: i) HepG2 cells which do not express membrane lectin specific for mannose were quite poorly transfected with pSV2Luc/mannosylated polylysine complexes, ii) HeLa cells which do not express membrane lectin specific for galactose were not transfected with pSV2Luc/lactosylated polylysine complexes. The transfection efficiency of HepG2 cells with pSV2Luc/lactosylated polylysine complexes was greatly enhanced either in the presence of chloroquine or in the presence of a fusogenic peptide. A 22-residue peptide derived from the influenza virus hemagglutinin HA2 N-terminal polypeptide that mimics the fusogenic activity of the virus, was selected. In the presence of the fusogenic peptide, the luciferase activity in HepG2 cells was 10 fold larger than that of cells transfected with pSV2Luc/lactosylated polylysine complexes in the presence of chloroquine.</p> </abstract> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
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