Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.
Identifieur interne : 000103 ( Hal/Corpus ); précédent : 000102; suivant : 000104Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.
Auteurs : P. Erbacher ; A. C. Roche ; M. Monsigny ; P. MidouxSource :
- Bioconjugate Chemistry [ 1043-1802 ] ; 1995.
Abstract
A DNA delivery system based on the use of polylysine substituted with small recognition signals, such as carbohydrate moieties specifically recognized by membrane lectins present in a given cell line, has been developed [Midoux et al. (1993) Nucleic Acids Res. 21, 871-878]. Human hepatoma (HepG2) cells which express a galactose-specific membrane lectin are efficiently transfected in the presence of chloroquine with pSV2Luc plasmid complexed with a lactosylated polylysine. The optimization of the parameters involved in the formation of DNA/glycosylated polylysine complexes leads to the following conclusions: a high gene transfer efficiency is reached when (i) DNA/glycosylated polylysine complexes are completely retarded when subjected to electrophoresis and when (ii) 31 +/- 4% or 40 +/- 8% of the amino groups of a polylysine having a degree of polymerization (DP) of 190 are substituted with lactosyl or beta-D-galactosyl residues, respectively. In addition, carbohydrate residues bound to polylysine decrease the electrostatic strength between plasmid DNA and glycosylated polylysine, suggesting that the strength of the electrostatic interactions between the plasmid and the glycosylated polylysine plays an important role in the efficiency of the gene expression. The optimal lactosylated polylysine conjugate (polylysine DP 190 substituted with 60 lactosyl residues) transfers a 5 kb and a 12 kb plasmid with a similar efficiency.
Url:
DOI: 10.1021/bc00034a010
Links to Exploration step
Hal:hal-02161414Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.</title><author><name sortKey="Erbacher, P" sort="Erbacher, P" uniqKey="Erbacher P" first="P" last="Erbacher">P. Erbacher</name></author><author><name sortKey="Roche, A C" sort="Roche, A C" uniqKey="Roche A" first="A. C." last="Roche">A. C. Roche</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno> <idno type="RNSR">196717612S</idno> <orgName>Centre de biophysique moléculaire</orgName> <orgName type="acronym">CBM</orgName> <date type="start">1967-01-01</date> <desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://cbm.cnrs-orleans.fr/</ref> </desc> <listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation> <relation name="UPR4301" active="#struct-303623" type="direct"></relation> <relation name="UPR4301" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno> <idno type="ISNI">0000000121581666 </idno> <orgName>Université d'Orléans</orgName> <orgName type="acronym">UO</orgName> <desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Monsigny, M" sort="Monsigny, M" uniqKey="Monsigny M" first="M." last="Monsigny">M. Monsigny</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno> <idno type="RNSR">196717612S</idno> <orgName>Centre de biophysique moléculaire</orgName> <orgName type="acronym">CBM</orgName> <date type="start">1967-01-01</date> <desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://cbm.cnrs-orleans.fr/</ref> </desc> <listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation> <relation name="UPR4301" active="#struct-303623" type="direct"></relation> <relation name="UPR4301" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno> <idno type="ISNI">0000000121581666 </idno> <orgName>Université d'Orléans</orgName> <orgName type="acronym">UO</orgName> <desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Midoux, P" sort="Midoux, P" uniqKey="Midoux P" first="P." last="Midoux">P. Midoux</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno> <idno type="RNSR">196717612S</idno> <orgName>Centre de biophysique moléculaire</orgName> <orgName type="acronym">CBM</orgName> <date type="start">1967-01-01</date> <desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://cbm.cnrs-orleans.fr/</ref> </desc> <listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation> <relation name="UPR4301" active="#struct-303623" type="direct"></relation> <relation name="UPR4301" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno> <idno type="ISNI">0000000121581666 </idno> <orgName>Université d'Orléans</orgName> <orgName type="acronym">UO</orgName> <desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-02161414</idno><idno type="halId">hal-02161414</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-02161414</idno><idno type="url">https://hal.archives-ouvertes.fr/hal-02161414</idno><idno type="doi">10.1021/bc00034a010</idno><date when="1995">1995</date><idno type="wicri:Area/Hal/Corpus">000103</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.</title><author><name sortKey="Erbacher, P" sort="Erbacher, P" uniqKey="Erbacher P" first="P" last="Erbacher">P. Erbacher</name></author><author><name sortKey="Roche, A C" sort="Roche, A C" uniqKey="Roche A" first="A. C." last="Roche">A. C. Roche</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno> <idno type="RNSR">196717612S</idno> <orgName>Centre de biophysique moléculaire</orgName> <orgName type="acronym">CBM</orgName> <date type="start">1967-01-01</date> <desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://cbm.cnrs-orleans.fr/</ref> </desc> <listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation> <relation name="UPR4301" active="#struct-303623" type="direct"></relation> <relation name="UPR4301" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno> <idno type="ISNI">0000000121581666 </idno> <orgName>Université d'Orléans</orgName> <orgName type="acronym">UO</orgName> <desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Monsigny, M" sort="Monsigny, M" uniqKey="Monsigny M" first="M." last="Monsigny">M. Monsigny</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno> <idno type="RNSR">196717612S</idno> <orgName>Centre de biophysique moléculaire</orgName> <orgName type="acronym">CBM</orgName> <date type="start">1967-01-01</date> <desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://cbm.cnrs-orleans.fr/</ref> </desc> <listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation> <relation name="UPR4301" active="#struct-303623" type="direct"></relation> <relation name="UPR4301" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno> <idno type="ISNI">0000000121581666 </idno> <orgName>Université d'Orléans</orgName> <orgName type="acronym">UO</orgName> <desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Midoux, P" sort="Midoux, P" uniqKey="Midoux P" first="P." last="Midoux">P. Midoux</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-451" status="VALID"> <idno type="IdRef">170121070</idno> <idno type="RNSR">196717612S</idno> <orgName>Centre de biophysique moléculaire</orgName> <orgName type="acronym">CBM</orgName> <date type="start">1967-01-01</date> <desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://cbm.cnrs-orleans.fr/</ref> </desc> <listRelation> <relation name="UPR4301" active="#struct-300297" type="direct"></relation> <relation name="UPR4301" active="#struct-303623" type="direct"></relation> <relation name="UPR4301" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UPR4301" active="#struct-300297" type="direct"><org type="institution" xml:id="struct-300297" status="VALID"> <idno type="IdRef">026402971</idno> <idno type="ISNI">0000000121581666 </idno> <orgName>Université d'Orléans</orgName> <orgName type="acronym">UO</orgName> <desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-orleans.fr/</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle><tutelle name="UPR4301" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author></analytic><idno type="DOI">10.1021/bc00034a010</idno><series><title level="j">Bioconjugate Chemistry</title><idno type="ISSN">1043-1802</idno><imprint><date type="datePub">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>A DNA delivery system based on the use of polylysine substituted with small recognition signals, such as carbohydrate moieties specifically recognized by membrane lectins present in a given cell line, has been developed [Midoux et al. (1993) Nucleic Acids Res. 21, 871-878]. Human hepatoma (HepG2) cells which express a galactose-specific membrane lectin are efficiently transfected in the presence of chloroquine with pSV2Luc plasmid complexed with a lactosylated polylysine. The optimization of the parameters involved in the formation of DNA/glycosylated polylysine complexes leads to the following conclusions: a high gene transfer efficiency is reached when (i) DNA/glycosylated polylysine complexes are completely retarded when subjected to electrophoresis and when (ii) 31 +/- 4% or 40 +/- 8% of the amino groups of a polylysine having a degree of polymerization (DP) of 190 are substituted with lactosyl or beta-D-galactosyl residues, respectively. In addition, carbohydrate residues bound to polylysine decrease the electrostatic strength between plasmid DNA and glycosylated polylysine, suggesting that the strength of the electrostatic interactions between the plasmid and the glycosylated polylysine plays an important role in the efficiency of the gene expression. The optimal lactosylated polylysine conjugate (polylysine DP 190 substituted with 60 lactosyl residues) transfers a 5 kb and a 12 kb plasmid with a similar efficiency.</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.</title> <author role="aut"> <persName> <forename type="first">P</forename> <surname>Erbacher</surname> </persName> <idno type="halauthorid">11515780</idno> </author> <author role="aut"> <persName> <forename type="first">A.C.</forename> <surname>Roche</surname> </persName> <idno type="halauthorid">116305</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">M.</forename> <surname>Monsigny</surname> </persName> <idno type="halauthorid">116306</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">P.</forename> <surname>Midoux</surname> </persName> <email type="md5">c8f24a3cf955469aa969463cc1db0798</email> <email type="domain">cnrs-orleans.fr</email> <idno type="idhal" notation="string">patrick-midoux</idno> <idno type="idhal" notation="numeric">176062</idno> <idno type="halauthorid">116547</idno> <affiliation ref="#struct-451"></affiliation> </author> <editor role="depositor"> <persName> <forename>Laëtitia</forename> <surname>LEGOUPIL</surname> </persName> <email type="md5">e9a3fa0bdfd22a9d0a2d2ac46e7772a2</email> <email type="domain">cnrs-orleans.fr</email> </editor> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2019-06-20 16:46:47</date> <date type="whenModified">2019-06-21 01:39:42</date> <date type="whenReleased">2019-06-20 16:46:47</date> <date type="whenProduced">1995</date> <ref type="externalLink" target="https://api.istex.fr/ark:/67375/TPS-F25JC71P-K/fulltext.pdf?sid=hal"></ref> </edition> <respStmt> <resp>contributor</resp> <name key="712555"> <persName> <forename>Laëtitia</forename> <surname>LEGOUPIL</surname> </persName> <email type="md5">e9a3fa0bdfd22a9d0a2d2ac46e7772a2</email> <email type="domain">cnrs-orleans.fr</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">hal-02161414</idno> <idno type="halUri">https://hal.archives-ouvertes.fr/hal-02161414</idno> <idno type="halBibtex">erbacher:hal-02161414</idno> <idno type="halRefHtml">Bioconjugate Chemistry, American Chemical Society, 1995, 6 (4), pp.401-10. ⟨10.1021/bc00034a010⟩</idno> <idno type="halRef">Bioconjugate Chemistry, American Chemical Society, 1995, 6 (4), pp.401-10. ⟨10.1021/bc00034a010⟩</idno> </publicationStmt> <seriesStmt> <idno type="stamp" n="CBM">Centre de biophysique moléculaire</idno> <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno> <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno> <idno type="stamp" n="UNIV-ORLEANS">Université d'Orléans</idno> </seriesStmt> <notesStmt> <note type="audience" n="2">International</note> <note type="popular" n="0">No</note> <note type="peer" n="1">Yes</note> </notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.</title> <author role="aut"> <persName> <forename type="first">P</forename> <surname>Erbacher</surname> </persName> <idno type="halauthorid">11515780</idno> </author> <author role="aut"> <persName> <forename type="first">A.C.</forename> <surname>Roche</surname> </persName> <idno type="halauthorid">116305</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">M.</forename> <surname>Monsigny</surname> </persName> <idno type="halauthorid">116306</idno> <affiliation ref="#struct-451"></affiliation> </author> <author role="aut"> <persName> <forename type="first">P.</forename> <surname>Midoux</surname> </persName> <email type="md5">c8f24a3cf955469aa969463cc1db0798</email> <email type="domain">cnrs-orleans.fr</email> <idno type="idhal" notation="string">patrick-midoux</idno> <idno type="idhal" notation="numeric">176062</idno> <idno type="halauthorid">116547</idno> <affiliation ref="#struct-451"></affiliation> </author> </analytic> <monogr> <idno type="halJournalId" status="VALID">11133</idno> <idno type="issn">1043-1802</idno> <idno type="eissn">1520-4812</idno> <title level="j">Bioconjugate Chemistry</title> <imprint> <publisher>American Chemical Society</publisher> <biblScope unit="volume">6</biblScope> <biblScope unit="issue">4</biblScope> <biblScope unit="pp">401-10</biblScope> <date type="datePub">1995</date> </imprint> </monogr> <idno type="doi">10.1021/bc00034a010</idno> <idno type="pubmed">7578360</idno> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode> <classCode scheme="halTypology" n="ART">Journal articles</classCode> </textClass> <abstract xml:lang="en"> <p>A DNA delivery system based on the use of polylysine substituted with small recognition signals, such as carbohydrate moieties specifically recognized by membrane lectins present in a given cell line, has been developed [Midoux et al. (1993) Nucleic Acids Res. 21, 871-878]. Human hepatoma (HepG2) cells which express a galactose-specific membrane lectin are efficiently transfected in the presence of chloroquine with pSV2Luc plasmid complexed with a lactosylated polylysine. The optimization of the parameters involved in the formation of DNA/glycosylated polylysine complexes leads to the following conclusions: a high gene transfer efficiency is reached when (i) DNA/glycosylated polylysine complexes are completely retarded when subjected to electrophoresis and when (ii) 31 +/- 4% or 40 +/- 8% of the amino groups of a polylysine having a degree of polymerization (DP) of 190 are substituted with lactosyl or beta-D-galactosyl residues, respectively. In addition, carbohydrate residues bound to polylysine decrease the electrostatic strength between plasmid DNA and glycosylated polylysine, suggesting that the strength of the electrostatic interactions between the plasmid and the glycosylated polylysine plays an important role in the efficiency of the gene expression. The optimal lactosylated polylysine conjugate (polylysine DP 190 substituted with 60 lactosyl residues) transfers a 5 kb and a 12 kb plasmid with a similar efficiency.</p> </abstract> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Hal/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000103 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Corpus/biblio.hfd -nk 000103 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Hal
|étape= Corpus
|type= RBID
|clé= Hal:hal-02161414
|texte= Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.
}}
| This area was generated with Dilib version V0.6.33. |  |