The in vitro production of prostanoids by cultured bovine articular chondrocytes
Identifieur interne : 000471 ( France/Analysis ); précédent : 000470; suivant : 000472The in vitro production of prostanoids by cultured bovine articular chondrocytes
Auteurs : D. Mitrovic [France] ; E. Mccall [France, Royaume-Uni] ; F. Dray [France]Source :
- Prostaglandins [ 0090-6980 ] ; 1982.
English descriptors
- Teeft :
- Arachidonic, Arachidonic acid, Articular, Articular chondrocytes, Assay, Cartilage, Cell suspensions, Chondrocytes, Culture medium, Ethyl acetate, Foetal calf serum, Indomethacin, January, Keto, Keto pgfla, Keto pgfla levels, Keto pgfta, Monolayer cultures, Pellet, Pge2, Pgf2a, Pgfa, Pgfla, Prostaglandin, Prostanoid production, Txb2.
Abstract
Abstract: Bovine articular chondrocytes, cultured as cell suspensions and monolayers, produced prostaglandin (PG) E2 and PGI2 (assayed as 6 keto PGF1α), rather less PGF2α and irregular quantities of thromboxane (Tx) B2. Addition of foetal calf serum to the medium greatly stimulated PG production (a sixfold increase in PGE2 and a twofold increase in 6 keto PGF1α).Prostanoid production by cell suspension grown in serum-free medium generally plateaued after 24 hours. In the presence of 20% foetal calf serum, prostanoid production in long-term monolayer cultures increased during the first 6 days of culture. Levels of PGE2α levels remained high. Indomethacin (10-6M) inhibited chondrocyte PG production both in the presence and absence of added arachidonic acid (10-4M). Prostanoids produced by chondrocytes may play a role in the modulation of cartilage metabolism in vivo.
Url:
DOI: 10.1016/0090-6980(82)90018-1
Affiliations:
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ISTEX:737699ACBD58312E2026B10F4EFBAAF77270F097Le document en format XML
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Mitrovic</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>U18 Inserm, Hopital Lariboisière, 6 rue Guy Patin 75010 Paris, and U.R.I.A., Institut Pasteur, 28 rue de Docteur Roux, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Mccall, E" sort="Mccall, E" uniqKey="Mccall E" first="E." last="Mccall">E. Mccall</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>U18 Inserm, Hopital Lariboisière, 6 rue Guy Patin 75010 Paris, and U.R.I.A., Institut Pasteur, 28 rue de Docteur Roux, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>3Present address: Dept. of Immunology, The Rayne Institute St. Thomas' Hospital, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Dray, F" sort="Dray, F" uniqKey="Dray F" first="F." last="Dray">F. Dray</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>U18 Inserm, Hopital Lariboisière, 6 rue Guy Patin 75010 Paris, and U.R.I.A., Institut Pasteur, 28 rue de Docteur Roux, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Prostaglandins</title><title level="j" type="abbrev">PROOLD</title><idno type="ISSN">0090-6980</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1982">1982</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="17">17</biblScope><biblScope unit="page" to="28">28</biblScope></imprint><idno type="ISSN">0090-6980</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0090-6980</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Arachidonic</term><term>Arachidonic acid</term><term>Articular</term><term>Articular chondrocytes</term><term>Assay</term><term>Cartilage</term><term>Cell suspensions</term><term>Chondrocytes</term><term>Culture medium</term><term>Ethyl acetate</term><term>Foetal calf serum</term><term>Indomethacin</term><term>January</term><term>Keto</term><term>Keto pgfla</term><term>Keto pgfla levels</term><term>Keto pgfta</term><term>Monolayer cultures</term><term>Pellet</term><term>Pge2</term><term>Pgf2a</term><term>Pgfa</term><term>Pgfla</term><term>Prostaglandin</term><term>Prostanoid production</term><term>Txb2</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Bovine articular chondrocytes, cultured as cell suspensions and monolayers, produced prostaglandin (PG) E2 and PGI2 (assayed as 6 keto PGF1α), rather less PGF2α and irregular quantities of thromboxane (Tx) B2. Addition of foetal calf serum to the medium greatly stimulated PG production (a sixfold increase in PGE2 and a twofold increase in 6 keto PGF1α).Prostanoid production by cell suspension grown in serum-free medium generally plateaued after 24 hours. In the presence of 20% foetal calf serum, prostanoid production in long-term monolayer cultures increased during the first 6 days of culture. Levels of PGE2α levels remained high. Indomethacin (10-6M) inhibited chondrocyte PG production both in the presence and absence of added arachidonic acid (10-4M). Prostanoids produced by chondrocytes may play a role in the modulation of cartilage metabolism in vivo.</div></front></TEI><affiliations><list><country><li>France</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Grand Londres</li><li>Île-de-France</li></region><settlement><li>Londres</li><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Mitrovic, D" sort="Mitrovic, D" uniqKey="Mitrovic D" first="D." last="Mitrovic">D. Mitrovic</name></region><name sortKey="Dray, F" sort="Dray, F" uniqKey="Dray F" first="F." last="Dray">F. Dray</name><name sortKey="Mccall, E" sort="Mccall, E" uniqKey="Mccall E" first="E." last="Mccall">E. Mccall</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Mccall, E" sort="Mccall, E" uniqKey="Mccall E" first="E." last="Mccall">E. Mccall</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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