Polyethylenimine‐mediated gene delivery: a mechanistic study
Identifieur interne : 000378 ( France/Analysis ); précédent : 000377; suivant : 000379Polyethylenimine‐mediated gene delivery: a mechanistic study
Auteurs : Antoine Kichler [France] ; Christian Leborgne [France] ; Emmanuel Coeytaux [France] ; Olivier Danos [France]Source :
- The Journal of Gene Medicine [ 1099-498X ] ; 2001-03.
English descriptors
- Teeft :
- Acidic, Assay, Assay buffer, Bioconjug chem, Biol, Biol chem, Cationic, Cationic polymers, Cell line, Cell lines, Chem, Chloroquine, Copyright, Cytosol, Dotap, Endocytic vesicles, Endosomes, Endosomolytic, Endosomolytic activity, Endosomolytic agents, Erythrocyte, Fusogenic peptides, Gene, Gene delivery, Gene transfer, Hela cells, Hemolytic activity, Hepg2, Hepg2 cells, Inhibitor, John wiley sons, Kichler, Lipid, Luciferase, Luciferase activity, Lysis, Mammalian cells, Monocationic lipid, Pei, Peptide, Plasmid, Polymer, Polyplexes, Present data show, Present results, Proc natl acad, Proton sponge effect, Reporter gene, Reporter gene expression, Same time, Transfected, Transfection, Transfection complexes, Transfection experiments, Transfection medium, Vesicle.
Abstract
Background: Ethylenimine polymers (PEIs) belong to one of the most efficient family of cationic compounds for delivery of plasmid DNA into mammalian cells. The high transfection efficiencies are obtained even in the absence of endosomolytic agents such as fusogenic peptides or chloroquine, which is in contrast to most of the other cationic polymers. It has been hypothesized that the efficiency of PEI is due to its capacity to buffer the endosomes. Methods: To investigate the importance of the acidification of endosomes during PEI‐mediated DNA transfer we used proton pump inhibitors such as bafilomycin A1 and concanamycin A. Moreover, we tested whether PEI is able to destabilize natural membranes per se at neutral or acidic pH by performing erythrocyte lysis assays. Results: PEI‐mediated transfection in the presence of bafilomycin A1 resulted in a 7–74‐fold decrease in reporter gene expression depending on the cell line used. In contrast, the efficiency of the monocationic lipid, DOTAP, was not importantly altered in the presence of the drug. Furthermore, the present data show that PEI cannot destabilize erythrocyte membranes, even at acidic pH, and that PEI, complexed or not to DNA, can increase the transfection efficiency of the cationic polymer, polylysine, when added at the same time to the cells. Conclusions: The transfection efficiency of PEIs partially relies on their ability to capture the protons which are transferred into the endosomes during their acidification. In addition, PEI is able to deliver significant amounts of DNA into cells and the DNA complexes involved in the expression of the transgene escape within 4 h from the endosomes. Copyright © 2001 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jgm.173
Affiliations:
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CNRS URA 1923, 1 Rue de l'Internationale, BP 60, F‐91002 Evry</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Évry (Essonne)</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Gene Medicine</title><title level="j" type="alt">JOURNAL OF GENE MEDICINE, THE</title><idno type="ISSN">1099-498X</idno><idno type="eISSN">1521-2254</idno><imprint><biblScope unit="vol">3</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="135">135</biblScope><biblScope unit="page" to="144">144</biblScope><biblScope unit="page-count">10</biblScope><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2001-03">2001-03</date></imprint><idno type="ISSN">1099-498X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1099-498X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acidic</term><term>Assay</term><term>Assay buffer</term><term>Bioconjug chem</term><term>Biol</term><term>Biol chem</term><term>Cationic</term><term>Cationic polymers</term><term>Cell line</term><term>Cell lines</term><term>Chem</term><term>Chloroquine</term><term>Copyright</term><term>Cytosol</term><term>Dotap</term><term>Endocytic vesicles</term><term>Endosomes</term><term>Endosomolytic</term><term>Endosomolytic activity</term><term>Endosomolytic agents</term><term>Erythrocyte</term><term>Fusogenic peptides</term><term>Gene</term><term>Gene delivery</term><term>Gene transfer</term><term>Hela cells</term><term>Hemolytic activity</term><term>Hepg2</term><term>Hepg2 cells</term><term>Inhibitor</term><term>John wiley sons</term><term>Kichler</term><term>Lipid</term><term>Luciferase</term><term>Luciferase activity</term><term>Lysis</term><term>Mammalian cells</term><term>Monocationic lipid</term><term>Pei</term><term>Peptide</term><term>Plasmid</term><term>Polymer</term><term>Polyplexes</term><term>Present data show</term><term>Present results</term><term>Proc natl acad</term><term>Proton sponge effect</term><term>Reporter gene</term><term>Reporter gene expression</term><term>Same time</term><term>Transfected</term><term>Transfection</term><term>Transfection complexes</term><term>Transfection experiments</term><term>Transfection medium</term><term>Vesicle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Ethylenimine polymers (PEIs) belong to one of the most efficient family of cationic compounds for delivery of plasmid DNA into mammalian cells. The high transfection efficiencies are obtained even in the absence of endosomolytic agents such as fusogenic peptides or chloroquine, which is in contrast to most of the other cationic polymers. It has been hypothesized that the efficiency of PEI is due to its capacity to buffer the endosomes. Methods: To investigate the importance of the acidification of endosomes during PEI‐mediated DNA transfer we used proton pump inhibitors such as bafilomycin A1 and concanamycin A. Moreover, we tested whether PEI is able to destabilize natural membranes per se at neutral or acidic pH by performing erythrocyte lysis assays. Results: PEI‐mediated transfection in the presence of bafilomycin A1 resulted in a 7–74‐fold decrease in reporter gene expression depending on the cell line used. In contrast, the efficiency of the monocationic lipid, DOTAP, was not importantly altered in the presence of the drug. Furthermore, the present data show that PEI cannot destabilize erythrocyte membranes, even at acidic pH, and that PEI, complexed or not to DNA, can increase the transfection efficiency of the cationic polymer, polylysine, when added at the same time to the cells. Conclusions: The transfection efficiency of PEIs partially relies on their ability to capture the protons which are transferred into the endosomes during their acidification. In addition, PEI is able to deliver significant amounts of DNA into cells and the DNA complexes involved in the expression of the transgene escape within 4 h from the endosomes. Copyright © 2001 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Évry (Essonne)</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Kichler, Antoine" sort="Kichler, Antoine" uniqKey="Kichler A" first="Antoine" last="Kichler">Antoine Kichler</name></region><name sortKey="Coeytaux, Emmanuel" sort="Coeytaux, Emmanuel" uniqKey="Coeytaux E" first="Emmanuel" last="Coeytaux">Emmanuel Coeytaux</name><name sortKey="Danos, Olivier" sort="Danos, Olivier" uniqKey="Danos O" first="Olivier" last="Danos">Olivier Danos</name><name sortKey="Kichler, Antoine" sort="Kichler, Antoine" uniqKey="Kichler A" first="Antoine" last="Kichler">Antoine Kichler</name><name sortKey="Kichler, Antoine" sort="Kichler, Antoine" uniqKey="Kichler A" first="Antoine" last="Kichler">Antoine Kichler</name><name sortKey="Leborgne, Christian" sort="Leborgne, Christian" uniqKey="Leborgne C" first="Christian" last="Leborgne">Christian Leborgne</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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