A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation
Identifieur interne : 000114 ( Main/Exploration ); précédent : 000113; suivant : 000115A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation
Auteurs : Yuguo Lei ; David V. SchafferSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2013.
Abstract
Human pluripotent stem cells can be cultured in vitro and differentiated into presumably all cell types of the human body, and they therefore represent highly promising cell sources for biomedical applications such as cell therapies, tissue engineering, and drug discovery. These applications require large numbers of high-quality cells, and we report an efficient, defined, scalable, and good manufacturing practice-compatible 3D system for the production of human pluripotent stem cells and their progeny. The ease of use and flexible scalability of this system makes it suitable for numerous applications from the laboratory toward the clinic.
Url:
DOI: 10.1073/pnas.1309408110
PubMed: 24248365
PubMed Central: 3876251
Affiliations:
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Schaffer</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Chemical Engineering,</nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff3">California Institute for Quantitative Biosciences</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Helen Wills Neuroscience Institute,<institution>University of California</institution>, Berkeley,<addr-line>CA</addr-line> 94720</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>Human pluripotent stem cells can be cultured in vitro and differentiated into presumably all cell types of the human body, and they therefore represent highly promising cell sources for biomedical applications such as cell therapies, tissue engineering, and drug discovery. These applications require large numbers of high-quality cells, and we report an efficient, defined, scalable, and good manufacturing practice-compatible 3D system for the production of human pluripotent stem cells and their progeny. The ease of use and flexible scalability of this system makes it suitable for numerous applications from the laboratory toward the clinic.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Lei, Yuguo" sort="Lei, Yuguo" uniqKey="Lei Y" first="Yuguo" last="Lei">Yuguo Lei</name><name sortKey="Schaffer, David V" sort="Schaffer, David V" uniqKey="Schaffer D" first="David V." last="Schaffer">David V. Schaffer</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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