Improved confocal characterization of targets in nuclei of cytogenetic preparations.
Identifieur interne : 000014 ( PubMed/Corpus ); précédent : 000013; suivant : 000015Improved confocal characterization of targets in nuclei of cytogenetic preparations.
Auteurs : Edmond Kahn ; Philippe Coullin ; Frédérique Frouin ; Andrew Todd-Pokropek ; Alain BernheimSource :
- Analytical and quantitative cytology and histology [ 0884-6812 ] ; 2002.
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Abstract
To show that cellular preparations requiring depth analysis of different domains stained by molecular cytogenetic methods (fluorescence in situ hybridization and primed in situ) can be improved by regularized factor analysis of medical image sequences (FAMIS) to isolate fluorescent probes by means of intensity depth profiles of fluorochromes, to track relevant DNA sequences (cosmids and centromeres) in cell nuclei during interphase and to improve the use of cytogenetic techniques resulting in flat preparations of whole cells that are assumed to preserve probe access to their targets.
PubMed: 12102131
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Improved confocal characterization of targets in nuclei of cytogenetic preparations.</title><author><name sortKey="Kahn, Edmond" sort="Kahn, Edmond" uniqKey="Kahn E" first="Edmond" last="Kahn">Edmond Kahn</name><affiliation><nlm:affiliation>Institut National de la Santé et de la Recherche Médicale U494, Centre Hospitalier Universitaire Pitié-Salpêtrière, Paris, France.</nlm:affiliation></affiliation></author><author><name sortKey="Coullin, Philippe" sort="Coullin, Philippe" uniqKey="Coullin P" first="Philippe" last="Coullin">Philippe Coullin</name></author><author><name sortKey="Frouin, Frederique" sort="Frouin, Frederique" uniqKey="Frouin F" first="Frédérique" last="Frouin">Frédérique Frouin</name></author><author><name sortKey="Todd Pokropek, Andrew" sort="Todd Pokropek, Andrew" uniqKey="Todd Pokropek A" first="Andrew" last="Todd-Pokropek">Andrew Todd-Pokropek</name></author><author><name sortKey="Bernheim, Alain" sort="Bernheim, Alain" uniqKey="Bernheim A" first="Alain" last="Bernheim">Alain Bernheim</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12102131</idno><idno type="pmid">12102131</idno><idno type="wicri:Area/PubMed/Corpus">00014</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">00014</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Improved confocal characterization of targets in nuclei of cytogenetic preparations.</title><author><name sortKey="Kahn, Edmond" sort="Kahn, Edmond" uniqKey="Kahn E" first="Edmond" last="Kahn">Edmond Kahn</name><affiliation><nlm:affiliation>Institut National de la Santé et de la Recherche Médicale U494, Centre Hospitalier Universitaire Pitié-Salpêtrière, Paris, France.</nlm:affiliation></affiliation></author><author><name sortKey="Coullin, Philippe" sort="Coullin, Philippe" uniqKey="Coullin P" first="Philippe" last="Coullin">Philippe Coullin</name></author><author><name sortKey="Frouin, Frederique" sort="Frouin, Frederique" uniqKey="Frouin F" first="Frédérique" last="Frouin">Frédérique Frouin</name></author><author><name sortKey="Todd Pokropek, Andrew" sort="Todd Pokropek, Andrew" uniqKey="Todd Pokropek A" first="Andrew" last="Todd-Pokropek">Andrew Todd-Pokropek</name></author><author><name sortKey="Bernheim, Alain" sort="Bernheim, Alain" uniqKey="Bernheim A" first="Alain" last="Bernheim">Alain Bernheim</name></author></analytic><series><title level="j">Analytical and quantitative cytology and histology</title><idno type="ISSN">0884-6812</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Nucleus (chemistry)</term><term>Cells, Cultured</term><term>Centromere (chemistry)</term><term>Cytogenetic Analysis (methods)</term><term>Humans</term><term>Image Processing, Computer-Assisted</term><term>In Situ Hybridization, Fluorescence (methods)</term><term>Male</term><term>Microscopy, Confocal (methods)</term><term>Sensitivity and Specificity</term><term>Signal Processing, Computer-Assisted</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Nucleus</term><term>Centromere</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cytogenetic Analysis</term><term>In Situ Hybridization, Fluorescence</term><term>Microscopy, Confocal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Humans</term><term>Image Processing, Computer-Assisted</term><term>Male</term><term>Sensitivity and Specificity</term><term>Signal Processing, Computer-Assisted</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To show that cellular preparations requiring depth analysis of different domains stained by molecular cytogenetic methods (fluorescence in situ hybridization and primed in situ) can be improved by regularized factor analysis of medical image sequences (FAMIS) to isolate fluorescent probes by means of intensity depth profiles of fluorochromes, to track relevant DNA sequences (cosmids and centromeres) in cell nuclei during interphase and to improve the use of cytogenetic techniques resulting in flat preparations of whole cells that are assumed to preserve probe access to their targets.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12102131</PMID><DateCompleted><Year>2003</Year><Month>01</Month><Day>06</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0884-6812</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>3</Issue><PubDate><Year>2002</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Analytical and quantitative cytology and histology</Title><ISOAbbreviation>Anal. Quant. Cytol. Histol.</ISOAbbreviation></Journal><ArticleTitle>Improved confocal characterization of targets in nuclei of cytogenetic preparations.</ArticleTitle><Pagination><MedlinePgn>178-84</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To show that cellular preparations requiring depth analysis of different domains stained by molecular cytogenetic methods (fluorescence in situ hybridization and primed in situ) can be improved by regularized factor analysis of medical image sequences (FAMIS) to isolate fluorescent probes by means of intensity depth profiles of fluorochromes, to track relevant DNA sequences (cosmids and centromeres) in cell nuclei during interphase and to improve the use of cytogenetic techniques resulting in flat preparations of whole cells that are assumed to preserve probe access to their targets.</AbstractText><AbstractText Label="STUDY DESIGN" NlmCategory="METHODS">3D sequences of images obtained by depth displacement in a confocal microscope were first analyzed by the FAMIS algorithm, which provides factor curves. Factor images then resulted from regularization methods that improve signal/noise ratio while preserving target contours.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Factor curves and regularized factor images helped analyze targets inside nuclei.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">It is possible to process preparations containing numerous spots (even when they are on different planes) to differentiate stained targets, to investigate depth differences and to improve visualization and detection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kahn</LastName><ForeName>Edmond</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institut National de la Santé et de la Recherche Médicale U494, Centre Hospitalier Universitaire Pitié-Salpêtrière, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Coullin</LastName><ForeName>Philippe</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Frouin</LastName><ForeName>Frédérique</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Todd-Pokropek</LastName><ForeName>Andrew</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bernheim</LastName><ForeName>Alain</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Quant Cytol Histol</MedlineTA><NlmUniqueID>8506819</NlmUniqueID><ISSNLinking>0884-6812</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002503" MajorTopicYN="N">Centromere</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020732" MajorTopicYN="N">Cytogenetic Analysis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007091" MajorTopicYN="N">Image Processing, Computer-Assisted</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017404" MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012815" MajorTopicYN="N">Signal Processing, Computer-Assisted</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>7</Month><Day>10</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>1</Month><Day>8</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>7</Month><Day>10</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12102131</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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