Laboratory Diagnosis of Amebiasis
Identifieur interne : 000137 ( Pmc/Curation ); précédent : 000136; suivant : 000138Laboratory Diagnosis of Amebiasis
Auteurs : Mehmet Tanyuksel ; William A. PetriSource :
- Clinical Microbiology Reviews [ 0893-8512 ] ; 2003.
Abstract
The detection of
Url:
DOI: 10.1128/CMR.16.4.713-729.2003
PubMed: 14557296
PubMed Central: 207118
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Laboratory Diagnosis of Amebiasis</title><author><name sortKey="Tanyuksel, Mehmet" sort="Tanyuksel, Mehmet" uniqKey="Tanyuksel M" first="Mehmet" last="Tanyuksel">Mehmet Tanyuksel</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Petri, William A" sort="Petri, William A" uniqKey="Petri W" first="William A." last="Petri">William A. Petri</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">14557296</idno><idno type="pmc">207118</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC207118</idno><idno type="RBID">PMC:207118</idno><idno type="doi">10.1128/CMR.16.4.713-729.2003</idno><date when="2003">2003</date><idno type="wicri:Area/Pmc/Corpus">000137</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000137</idno><idno type="wicri:Area/Pmc/Curation">000137</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000137</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Laboratory Diagnosis of Amebiasis</title><author><name sortKey="Tanyuksel, Mehmet" sort="Tanyuksel, Mehmet" uniqKey="Tanyuksel M" first="Mehmet" last="Tanyuksel">Mehmet Tanyuksel</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Petri, William A" sort="Petri, William A" uniqKey="Petri W" first="William A." last="Petri">William A. Petri</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Clinical Microbiology Reviews</title><idno type="ISSN">0893-8512</idno><idno type="eISSN">1098-6618</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The detection of <italic>Entamoeba histolytica</italic>, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of <italic>E. histolytica</italic> infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of <italic>E. histolytica</italic>, there is great potential for further understanding the pathogenesis of amebiasis. Molecular biology-based diagnosis may become the technique of choice in the future because establishment of these protozoa in culture is still not a routine clinical laboratory process. In all cases, combination of serologic tests with detection of the parasite (by antigen detection or PCR) offers the best approach to diagnosis, while PCR techniques remain impractical in many developing country settings. The detection of amebic markers in serum in patients with amebic colitis and liver abscess appears promising but is still only a research tool. On the other hand, stool antigen detection tests offer a practical, sensitive, and specific way for the clinical laboratory to detect intestinal <italic>E. histolytica</italic>. All the current tests suffer from the fact that the antigens detected are denatured by fixation of the stool specimen, limiting testing to fresh or frozen samples.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Microbiol Rev</journal-id><journal-id journal-id-type="publisher-id">cmr</journal-id><journal-title>Clinical Microbiology Reviews</journal-title><issn pub-type="ppub">0893-8512</issn><issn pub-type="epub">1098-6618</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">14557296</article-id><article-id pub-id-type="pmc">207118</article-id><article-id pub-id-type="publisher-id">0073</article-id><article-id pub-id-type="doi">10.1128/CMR.16.4.713-729.2003</article-id><article-categories><subj-group subj-group-type="heading"><subject>Reviews</subject></subj-group></article-categories><title-group><article-title>Laboratory Diagnosis of Amebiasis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tanyuksel</surname><given-names>Mehmet</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Petri</surname><given-names>William A.</given-names><suffix>Jr.</suffix></name><xref ref-type="aff" rid="aff1">2</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1">Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Etlik, Ankara 06018, Turkey,<label>1</label> Department of Internal Medicine, University of Virginia, Charlottesville, Virginia 22908-1340<label>2</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Division of Infectious Diseases and International Health, University of Virginia Health System, MR4 Building, room 2115, Lane Rd., P.O. Box 801340, Charlottesville, VA 22908-1340. Phone: (434) 924-5621. Fax: (434) 924-0075. E-mail: <email>wap3g@virginia.edu</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>10</month><year>2003</year></pub-date><volume>16</volume><issue>4</issue><fpage>713</fpage><lpage>729</lpage><copyright-statement>Copyright © 2003, American Society for Microbiology</copyright-statement><copyright-year>2003</copyright-year><abstract><p>The detection of <italic>Entamoeba histolytica</italic>, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of <italic>E. histolytica</italic> infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of <italic>E. histolytica</italic>, there is great potential for further understanding the pathogenesis of amebiasis. Molecular biology-based diagnosis may become the technique of choice in the future because establishment of these protozoa in culture is still not a routine clinical laboratory process. In all cases, combination of serologic tests with detection of the parasite (by antigen detection or PCR) offers the best approach to diagnosis, while PCR techniques remain impractical in many developing country settings. The detection of amebic markers in serum in patients with amebic colitis and liver abscess appears promising but is still only a research tool. On the other hand, stool antigen detection tests offer a practical, sensitive, and specific way for the clinical laboratory to detect intestinal <italic>E. histolytica</italic>. All the current tests suffer from the fact that the antigens detected are denatured by fixation of the stool specimen, limiting testing to fresh or frozen samples.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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