In situ analysis of trifluorothymidine-resistant (TFTr) mutants of L5178Y/TK+/− mouse lymphoma cells
Identifieur interne : 000C26 ( Main/Exploration ); précédent : 000C25; suivant : 000C27In situ analysis of trifluorothymidine-resistant (TFTr) mutants of L5178Y/TK+/− mouse lymphoma cells
Auteurs : Martha M. Moore [États-Unis] ; Donald Clive [États-Unis] ; Barry E. Howard [États-Unis] ; A. Gail Batson [États-Unis] ; Nancy T. Turner [États-Unis]Source :
- Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis [ 0027-5107 ] ; 1985.
English descriptors
- Teeft :
- Agar, Agar concentration, Amacher, Assay, Automatic colony counter, Budr, Budr selection, Chromosomal, Chromosome, Chromosome aberrations, Clive, Colony size, Colony size distribution, Cytogenetic, Cytogenetic analysis, Days expression, Environmental protection agency, Histogram, Hozier, Jacobson, Kinase, Kinetics, Large colony, Lymphoma, Mammalian cells, Methyl iodide, Methyl methanesulfonate, Mouse lymphoma, Mouse lymphoma cells, Mutagen, Mutagenesis, Mutagenicity, Mutant, Mutant frequency, Mutant induction, Mutant selection, Mutation, Paillet, Phenotype, Relative proportion, Relative proportions, Research triangle park, Significant proportion, Size discriminator, Size distribution, Small colonies, Small colony, Test systems, Thymidine, Thymidine kinase locus, Toxicity, Unselected population, Viable count plates.
Abstract
Abstract: TFTr mutants of L5178Y/TK+/− mouse lymphoma cells are analyzed as they appear in situ following cloning and incubation for 9–11 days in soft agar cloning medium. These TFTr mutants can be divided by colony size into σ, small colony, and λ, large colony, mutants. The use of a size discriminator on an automatic colony counter allows the production of histograms to evaluate the size distribution of colonies on a plate. The evaluation of these size distribution curves provides insight into the properties of σ and λ mutants. From these analyses several conclusions may be drawn. The σ phenotype is preferentially associated with the TFTr subpopulation of a treated culture. The σ phenotype is not an artifact of delayed toxicity following treatment. The frequency of quantifiable σ mutants is not affected by agar concentrations between 0.20% and 0.45% in the cloning medium. TFTr σ mutants are produced spontaneously and can be induced by a variety of mutagens. The decline in overall detectable mutant frequency observed for some mutagens with increasing time after treatment is due to the decline in σ mutant frequency. The quantitation of both σ and λ mutants is thus useful in obtaining maximum utility of the information provided by the L5178Y/TK+/− mouse lymphoma assay.
Url:
DOI: 10.1016/0027-5107(85)90193-9
Affiliations:
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These TFTr mutants can be divided by colony size into σ, small colony, and λ, large colony, mutants. The use of a size discriminator on an automatic colony counter allows the production of histograms to evaluate the size distribution of colonies on a plate. The evaluation of these size distribution curves provides insight into the properties of σ and λ mutants. From these analyses several conclusions may be drawn. The σ phenotype is preferentially associated with the TFTr subpopulation of a treated culture. The σ phenotype is not an artifact of delayed toxicity following treatment. The frequency of quantifiable σ mutants is not affected by agar concentrations between 0.20% and 0.45% in the cloning medium. TFTr σ mutants are produced spontaneously and can be induced by a variety of mutagens. The decline in overall detectable mutant frequency observed for some mutagens with increasing time after treatment is due to the decline in σ mutant frequency. The quantitation of both σ and λ mutants is thus useful in obtaining maximum utility of the information provided by the L5178Y/TK+/− mouse lymphoma assay.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Moore, Martha M" sort="Moore, Martha M" uniqKey="Moore M" first="Martha M." last="Moore">Martha M. Moore</name></region><name sortKey="Batson, A Gail" sort="Batson, A Gail" uniqKey="Batson A" first="A. Gail" last="Batson">A. Gail Batson</name><name sortKey="Clive, Donald" sort="Clive, Donald" uniqKey="Clive D" first="Donald" last="Clive">Donald Clive</name><name sortKey="Howard, Barry E" sort="Howard, Barry E" uniqKey="Howard B" first="Barry E." last="Howard">Barry E. Howard</name><name sortKey="Turner, Nancy T" sort="Turner, Nancy T" uniqKey="Turner N" first="Nancy T." last="Turner">Nancy T. 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