Prolonged Hormone Secretion from Neuroendocrine Cells of Aplysia is Indepentdent of Extracellular Calcium
Identifieur interne : 000792 ( Istex/Corpus ); précédent : 000791; suivant : 000793Prolonged Hormone Secretion from Neuroendocrine Cells of Aplysia is Indepentdent of Extracellular Calcium
Auteurs : Nancy L. Wayne ; Jiin Kim ; Eugene LeeSource :
- Journal of Neuroendocrinology [ 0953-8194 ] ; 1998-07.
English descriptors
- Teeft :
- Abdominal ganglion, Acad, Action potentials, Afterdischarge, Afterdischarges, Aplysia, Aplysia californica, Bapta, Baseline, Blackwell science, Calcium, Calcium channels, Calcium ionophore, Calcium ionophores, Californica, Cell afterdischarge, Cell neurons, Chelators, Comp physiol, Control group, Control preparations, Depolarization, Egta, Electrical activity, Electrical afterdischarge, Electrical stimulation, Exocytosis, Experimental group, Extracellular, Extracellular calcium, Extracellular calcium chelators, Ganglion, Hormone secretion, Intracellular, Intracellular stores, Ionophore, Kaczmarek, Kinase, Natl, Ndings, Neuroendocrine, Neuroendocrine cells, Neuroendocrinology, Neuron, Neurosecretion, Peptide, Physiol, Present study, Pretreatment period, Proc, Proc natl acad, Protein kinase, Retinal neuron, Secretion, Secretory, Total amount, Treatment period, Vascular space.
Abstract
The role of Ca2+ from extracellular and intracellular sources in stimulating neurosecretion was investigated in four experiments using neuroendocrine bag cells of the marine mollusk Aplysia. (i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co2+‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca2+ from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca2+ is not required for maintenance of ELH release. (ii) Substitution of Ba2+ for Ca2+ has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca2+. We showed that this treatment also stimulates ELH secretion, suggesting that Ca2+ release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca2+ levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca2+ across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca2+ was from intracellular compartments, ELH secretion was stimulated. Taken together, these findings are consistent with the hypothesis that release of Ca2+ from intracellular stores is sufficient to stimulate ELH secretion.
Url:
DOI: 10.1046/j.1365-2826.1998.00235.x
Links to Exploration step
ISTEX:330A7CFB78C173DFD496EB9B87F10D1FB3C10711Le document en format XML
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Wayne</name><affiliation><mods:affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</mods:affiliation></affiliation></author><author><name sortKey="Kim, Jiin" sort="Kim, Jiin" uniqKey="Kim J" first="Jiin" last="Kim">Jiin Kim</name><affiliation><mods:affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</mods:affiliation></affiliation></author><author><name sortKey="Lee, Eugene" sort="Lee, Eugene" uniqKey="Lee E" first="Eugene" last="Lee">Eugene Lee</name><affiliation><mods:affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:330A7CFB78C173DFD496EB9B87F10D1FB3C10711</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1046/j.1365-2826.1998.00235.x</idno><idno type="url">https://api.istex.fr/document/330A7CFB78C173DFD496EB9B87F10D1FB3C10711/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000792</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000792</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Prolonged Hormone Secretion from Neuroendocrine Cells of <hi rend="italic">Aplysia</hi> is Indepentdent of Extracellular Calcium</title><author><name sortKey="Wayne, Nancy L" sort="Wayne, Nancy L" uniqKey="Wayne N" first="Nancy L." last="Wayne">Nancy L. 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(i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co2+‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca2+ from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca2+ is not required for maintenance of ELH release. (ii) Substitution of Ba2+ for Ca2+ has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca2+. We showed that this treatment also stimulates ELH secretion, suggesting that Ca2+ release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca2+ levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca2+ across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca2+ was from intracellular compartments, ELH secretion was stimulated. 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Wayne</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</json:string></affiliations></json:item><json:item><name>Jiin Kim</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</json:string></affiliations></json:item><json:item><name>Eugene Lee</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</json:string></affiliations></json:item></author><articleId><json:string>JNE235</json:string></articleId><arkIstex>ark:/67375/WNG-4J7KWLWM-5</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The role of Ca2+ from extracellular and intracellular sources in stimulating neurosecretion was investigated in four experiments using neuroendocrine bag cells of the marine mollusk Aplysia. (i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co2+‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca2+ from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca2+ is not required for maintenance of ELH release. (ii) Substitution of Ba2+ for Ca2+ has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca2+. We showed that this treatment also stimulates ELH secretion, suggesting that Ca2+ release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca2+ levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca2+ across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca2+ was from intracellular compartments, ELH secretion was stimulated. Taken together, these findings are consistent with the hypothesis that release of Ca2+ from intracellular stores is sufficient to stimulate ELH secretion.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>207</abstractWordCount><abstractCharCount>1449</abstractCharCount><keywordCount>0</keywordCount><score>9.484</score><pdfWordCount>6679</pdfWordCount><pdfCharCount>42497</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>596 x 842 pts (A4)</pdfPageSize></qualityIndicators><title>Prolonged Hormone Secretion from Neuroendocrine Cells of Aplysia is Indepentdent of Extracellular Calcium</title><pmid><json:string>9700680</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Journal of Neuroendocrinology</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1365-2826</json:string></doi><issn><json:string>0953-8194</json:string></issn><eissn><json:string>1365-2826</json:string></eissn><publisherId><json:string>JNE</json:string></publisherId><volume>10</volume><issue>7</issue><pages><first>529</first><last>537</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>10s</json:string><json:string>1998</json:string></date><geogName></geogName><orgName><json:string>Blackwell Science Ltd</json:string><json:string>Grass Instruments</json:string><json:string>Hitachi Denshi, Ltd</json:string><json:string>Molecular Probes, Inc.</json:string><json:string>Sigma Chemical Co.</json:string><json:string>Apple Computer, Inc., Cupertino</json:string><json:string>Baxter Healthcare Corp., Glendale</json:string><json:string>World Precision Instruments, Inc., Sarasota</json:string><json:string>Department of Physiology, UCLA School of Medicine</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Unless</json:string><json:string>Aplysia</json:string><json:string>N. 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(i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co<hi rend="superscript">2+</hi>‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca<hi rend="superscript">2+</hi> from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca<hi rend="superscript">2+</hi> is not required for maintenance of ELH release. (ii) Substitution of Ba<hi rend="superscript">2+</hi> for Ca<hi rend="superscript">2+</hi> has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca<hi rend="superscript">2+</hi>. We showed that this treatment also stimulates ELH secretion, suggesting that Ca<hi rend="superscript">2+</hi> release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca<hi rend="superscript">2+</hi> levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca<hi rend="superscript">2+</hi> across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca<hi rend="superscript">2+</hi> was from intracellular compartments, ELH secretion was stimulated. Taken together, these findings are consistent with the hypothesis that release of Ca<hi rend="superscript">2+</hi> from intracellular stores is sufficient to stimulate ELH secretion.</p></abstract><textClass><keywords rend="tocHeading1"><term>Original Article</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/330A7CFB78C173DFD496EB9B87F10D1FB3C10711/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd, UK</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2826</doi><issn type="print">0953-8194</issn><issn type="electronic">1365-2826</issn><idGroup><id type="product" value="JNE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF NEUROENDOCRINOLOGY">Journal of Neuroendocrinology</title><title type="short">Journal of Neuroendocrinology</title></titleGroup></publicationMeta><publicationMeta level="part" position="07007"><doi origin="wiley">10.1111/jne.1998.10.issue-7</doi><numberingGroup><numbering type="journalVolume" number="10">10</numbering><numbering type="journalIssue" number="7">7</numbering></numberingGroup><coverDate startDate="1998-07">July 1998</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0052900" status="forIssue"><doi origin="wiley">10.1046/j.1365-2826.1998.00235.x</doi><idGroup><id type="unit" value="JNE235"></id></idGroup><titleGroup><title type="tocHeading1">Original Article</title></titleGroup><eventGroup><event type="firstOnline" date="2001-12-25"></event><event type="publishedOnlineFinalForm" date="2001-12-25"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:Header result:Header" date="2010-03-16"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-31"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="529">529</numbering><numbering type="pageLast" number="537">537</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:JNE.JNE235.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="50"></count><count type="wordTotal" number="7981"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Prolonged Hormone Secretion from Neuroendocrine Cells of <i>Aplysia</i> is Indepentdent of Extracellular Calcium</title><title type="shortAuthors">Wayne</title><title type="short">Calcium and neurosecretion</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Nancy L.</givenNames><familyName>Wayne</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Jiin</givenNames><familyName>Kim</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Eugene</givenNames><familyName>Lee</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="US"><unparsedAffiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The role of Ca<sup>2+</sup> from extracellular and intracellular sources in stimulating neurosecretion was investigated in four experiments using neuroendocrine bag cells of the marine mollusk <i>Aplysia</i>. (i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co<sup>2+</sup>‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca<sup>2+</sup> from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca<sup>2+</sup> is not required for maintenance of ELH release. (ii) Substitution of Ba<sup>2+</sup> for Ca<sup>2+</sup> has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca<sup>2+</sup>. We showed that this treatment also stimulates ELH secretion, suggesting that Ca<sup>2+</sup> release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca<sup>2+</sup> levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca<sup>2+</sup> across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca<sup>2+</sup> was from intracellular compartments, ELH secretion was stimulated. Taken together, these findings are consistent with the hypothesis that release of Ca<sup>2+</sup> from intracellular stores is sufficient to stimulate ELH secretion.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Prolonged Hormone Secretion from Neuroendocrine Cells of Aplysia is Indepentdent of Extracellular Calcium</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Calcium and neurosecretion</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Prolonged Hormone Secretion from Neuroendocrine Cells of Aplysia is Indepentdent of Extracellular Calcium</title></titleInfo><name type="personal"><namePart type="given">Nancy L.</namePart><namePart type="family">Wayne</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jiin</namePart><namePart type="family">Kim</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eugene</namePart><namePart type="family">Lee</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, CA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Science Ltd, UK</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1998-07</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">4</extent><extent unit="tables">0</extent><extent unit="formulas">0</extent><extent unit="references">50</extent><extent unit="linksCrossRef">0</extent><extent unit="words">7981</extent></physicalDescription><abstract lang="en">The role of Ca2+ from extracellular and intracellular sources in stimulating neurosecretion was investigated in four experiments using neuroendocrine bag cells of the marine mollusk Aplysia. (i) Bag cells were treated with either an extracellular calcium chelator (BAPTA) or Co2+‐substitution within 30 s after onset of an electrical afterdischarge to prevent influx of Ca2+ from extracellular fluid. These treatments shortened the duration of the afterdischarge, but did not significantly affect the overall pattern or total amount of egg laying hormone (ELH) secretion, suggesting that extracellular Ca2+ is not required for maintenance of ELH release. (ii) Substitution of Ba2+ for Ca2+ has previously been shown to support bag cell afterdischarges that trigger transient elevations in intracellular Ca2+. We showed that this treatment also stimulates ELH secretion, suggesting that Ca2+ release from intracellular stores can stimulate ELH secretion. (iii) To raise intracellular Ca2+ levels in the absence of an afterdischarge, the calcium ionophore X537A was used to transport Ca2+ across plasma and organelle membranes. When this treatment was combined with extracellular calcium chelators so that the only source of Ca2+ was from intracellular compartments, ELH secretion was stimulated. Taken together, these findings are consistent with the hypothesis that release of Ca2+ from intracellular stores is sufficient to stimulate ELH secretion.</abstract><relatedItem type="host"><titleInfo><title>Journal of Neuroendocrinology</title></titleInfo><titleInfo type="abbreviated"><title>Journal of Neuroendocrinology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0953-8194</identifier><identifier type="eISSN">1365-2826</identifier><identifier type="DOI">10.1111/(ISSN)1365-2826</identifier><identifier type="PublisherID">JNE</identifier><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>529</start><end>537</end></extent></part></relatedItem><identifier type="istex">330A7CFB78C173DFD496EB9B87F10D1FB3C10711</identifier><identifier type="ark">ark:/67375/WNG-4J7KWLWM-5</identifier><identifier type="DOI">10.1046/j.1365-2826.1998.00235.x</identifier><identifier type="ArticleID">JNE235</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Science Ltd, UK</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/330A7CFB78C173DFD496EB9B87F10D1FB3C10711/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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