Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of Aplysia californica to Inhibitors of Protein Kinase A and Protein Kinase C
Identifieur interne : 000637 ( Istex/Corpus ); précédent : 000636; suivant : 000638Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of Aplysia californica to Inhibitors of Protein Kinase A and Protein Kinase C
Auteurs : Nancy L. Wayne ; Yun J. Kim ; Rocio J. Yong-MontenegroSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1998.
English descriptors
- Teeft :
- Abdominal ganglion, Academic press, Action potentials, Adenylate cyclase, Afterdischarge, Aplysia, Aplysia californica, Aplysia neurons, Assay, Calcium action potentials, Calcium channels, Californica, Camp production, Cell neurons, Comp, Consecutive years, Copyright, Cyclic, Diacylglycerol, Diacylglycerol pathways, Dos, Electrical stimulation, Ganglion, Hormone secretion, Inhibitor, Kaczmarek, Kinase, Membrane excitability, Ndings support, Neuroendocrine, Neuroendocrine cells, Neuron, Neurosci, Neurosecretory, Nonbreeding, Nonbreeding season, Nonbreeding seasons, Pathway, Peptide, Peptidergic neurons, Physiol, Present study, Previous work, Protein kinase, Protein kinases, Seasonal changes, Seasonal fluctuation, Seasonal regulation, Seasonal response, Secondmessenger, Secondmessenger systems, Secretion, Strumwasser, Total amount, Total number, Untreated controls, Water temperature.
Abstract
Abstract: The neuroendocrine bag cells ofAplysiaprovide an excellent model system for exploring the roles of second-messenger pathways regulating peptide hormone secretion. Both the cAMP and diacylglycerol second-messenger systems and their associated protein kinases (PKA and PKC) are involved in regulating membrane excitability in bag-cell neurons ofAplysia.The purpose of the present set of experiments was to determine if PKA and PKC also play roles in regulating egg laying hormone (ELH) secretion from bag-cell neurons. Abdominal ganglia with attached bag-cell clusters and connective nerves were dissected from reproductively matureAplysia,and ELH secretion in response to electrically stimulated afterdischarges was measured by RIA. ELH secretion from bag cells treated with protein-kinase inhibitors (Rp-cAMPS to inhibit PKA; H-7 to inhibit PKC) was compared to that from untreated controls. Our experiments showed that 100 μM Rp-cAMPS significantly attenuated ELH secretion during the nonbreeding seasons (winter and spring) of 2 consecutive years. This suggested a role for PKA in regulating ELH secretion. However, Rp-cAMPS had no effect on ELH secretion during the breeding seasons (summer and fall) of 2 consecutive years, even when the dose of Rp-cAMPS was increased to 200 μM. These findings indicate that there is a seasonal fluctuation in responsiveness to PKA inhibition. We also investigated if there was a seasonal fluctuation in the ability of the PKC inhibitor H-7 to suppress ELH secretion. During the nonbreeding season, 10–100 μM H-7 significantly inhibited ELH secretion, but during the breeding season, only the highest dose (100 μM) of H-7 inhibited ELH release. These results confirm that PKC plays a role in regulating ELH secretion and indicate that there is a seasonal fluctuation in responsiveness to PKC inhibition. Overall, our findings suggest that both the cAMP and diacylglycerol second-messenger pathways are regulated on a seasonal basis.
Url:
DOI: 10.1006/gcen.1997.7040
Links to Exploration step
ISTEX:39E36B3482B080ACFCD87AA71833CE43B5FC91F8Le document en format XML
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Wayne</name><affiliation><mods:affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</mods:affiliation></affiliation></author><author><name sortKey="Kim, Yun J" sort="Kim, Yun J" uniqKey="Kim Y" first="Yun J." last="Kim">Yun J. Kim</name><affiliation><mods:affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</mods:affiliation></affiliation></author><author><name sortKey="Yong Montenegro, Rocio J" sort="Yong Montenegro, Rocio J" uniqKey="Yong Montenegro R" first="Rocio J." last="Yong-Montenegro">Rocio J. Yong-Montenegro</name><affiliation><mods:affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="ISSN">0016-6480</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">109</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="356">356</biblScope><biblScope unit="page" to="365">365</biblScope></imprint><idno type="ISSN">0016-6480</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0016-6480</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Abdominal ganglion</term><term>Academic press</term><term>Action potentials</term><term>Adenylate cyclase</term><term>Afterdischarge</term><term>Aplysia</term><term>Aplysia californica</term><term>Aplysia neurons</term><term>Assay</term><term>Calcium action potentials</term><term>Calcium channels</term><term>Californica</term><term>Camp production</term><term>Cell neurons</term><term>Comp</term><term>Consecutive years</term><term>Copyright</term><term>Cyclic</term><term>Diacylglycerol</term><term>Diacylglycerol pathways</term><term>Dos</term><term>Electrical stimulation</term><term>Ganglion</term><term>Hormone secretion</term><term>Inhibitor</term><term>Kaczmarek</term><term>Kinase</term><term>Membrane excitability</term><term>Ndings support</term><term>Neuroendocrine</term><term>Neuroendocrine cells</term><term>Neuron</term><term>Neurosci</term><term>Neurosecretory</term><term>Nonbreeding</term><term>Nonbreeding season</term><term>Nonbreeding seasons</term><term>Pathway</term><term>Peptide</term><term>Peptidergic neurons</term><term>Physiol</term><term>Present study</term><term>Previous work</term><term>Protein kinase</term><term>Protein kinases</term><term>Seasonal changes</term><term>Seasonal fluctuation</term><term>Seasonal regulation</term><term>Seasonal response</term><term>Secondmessenger</term><term>Secondmessenger systems</term><term>Secretion</term><term>Strumwasser</term><term>Total amount</term><term>Total number</term><term>Untreated controls</term><term>Water temperature</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The neuroendocrine bag cells ofAplysiaprovide an excellent model system for exploring the roles of second-messenger pathways regulating peptide hormone secretion. Both the cAMP and diacylglycerol second-messenger systems and their associated protein kinases (PKA and PKC) are involved in regulating membrane excitability in bag-cell neurons ofAplysia.The purpose of the present set of experiments was to determine if PKA and PKC also play roles in regulating egg laying hormone (ELH) secretion from bag-cell neurons. Abdominal ganglia with attached bag-cell clusters and connective nerves were dissected from reproductively matureAplysia,and ELH secretion in response to electrically stimulated afterdischarges was measured by RIA. ELH secretion from bag cells treated with protein-kinase inhibitors (Rp-cAMPS to inhibit PKA; H-7 to inhibit PKC) was compared to that from untreated controls. Our experiments showed that 100 μM Rp-cAMPS significantly attenuated ELH secretion during the nonbreeding seasons (winter and spring) of 2 consecutive years. This suggested a role for PKA in regulating ELH secretion. However, Rp-cAMPS had no effect on ELH secretion during the breeding seasons (summer and fall) of 2 consecutive years, even when the dose of Rp-cAMPS was increased to 200 μM. These findings indicate that there is a seasonal fluctuation in responsiveness to PKA inhibition. We also investigated if there was a seasonal fluctuation in the ability of the PKC inhibitor H-7 to suppress ELH secretion. During the nonbreeding season, 10–100 μM H-7 significantly inhibited ELH secretion, but during the breeding season, only the highest dose (100 μM) of H-7 inhibited ELH release. These results confirm that PKC plays a role in regulating ELH secretion and indicate that there is a seasonal fluctuation in responsiveness to PKC inhibition. Overall, our findings suggest that both the cAMP and diacylglycerol second-messenger pathways are regulated on a seasonal basis.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>aplysia</json:string><json:string>afterdischarge</json:string><json:string>kinase</json:string><json:string>kaczmarek</json:string><json:string>nonbreeding</json:string><json:string>neuron</json:string><json:string>action potentials</json:string><json:string>nonbreeding season</json:string><json:string>pathway</json:string><json:string>cell neurons</json:string><json:string>cyclic</json:string><json:string>academic press</json:string><json:string>californica</json:string><json:string>copyright</json:string><json:string>physiol</json:string><json:string>protein kinase</json:string><json:string>comp</json:string><json:string>total number</json:string><json:string>membrane excitability</json:string><json:string>neurosci</json:string><json:string>ganglion</json:string><json:string>secondmessenger</json:string><json:string>diacylglycerol</json:string><json:string>strumwasser</json:string><json:string>neurosecretory</json:string><json:string>electrical stimulation</json:string><json:string>neuroendocrine</json:string><json:string>aplysia californica</json:string><json:string>peptide</json:string><json:string>secretion</json:string><json:string>inhibitor</json:string><json:string>nonbreeding seasons</json:string><json:string>abdominal ganglion</json:string><json:string>assay</json:string><json:string>protein kinases</json:string><json:string>total amount</json:string><json:string>aplysia neurons</json:string><json:string>neuroendocrine cells</json:string><json:string>seasonal changes</json:string><json:string>untreated controls</json:string><json:string>dos</json:string><json:string>present study</json:string><json:string>seasonal fluctuation</json:string><json:string>water temperature</json:string><json:string>seasonal response</json:string><json:string>ndings support</json:string><json:string>previous work</json:string><json:string>secondmessenger systems</json:string><json:string>seasonal regulation</json:string><json:string>calcium channels</json:string><json:string>hormone secretion</json:string><json:string>calcium action potentials</json:string><json:string>camp production</json:string><json:string>adenylate cyclase</json:string><json:string>diacylglycerol pathways</json:string><json:string>consecutive years</json:string><json:string>peptidergic neurons</json:string></teeft></keywords><author><json:item><name>Nancy L. Wayne</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</json:string></affiliations></json:item><json:item><name>Yun J. Kim</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</json:string></affiliations></json:item><json:item><name>Rocio J. Yong-Montenegro</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-1GW00B1C-X</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The neuroendocrine bag cells ofAplysiaprovide an excellent model system for exploring the roles of second-messenger pathways regulating peptide hormone secretion. Both the cAMP and diacylglycerol second-messenger systems and their associated protein kinases (PKA and PKC) are involved in regulating membrane excitability in bag-cell neurons ofAplysia.The purpose of the present set of experiments was to determine if PKA and PKC also play roles in regulating egg laying hormone (ELH) secretion from bag-cell neurons. Abdominal ganglia with attached bag-cell clusters and connective nerves were dissected from reproductively matureAplysia,and ELH secretion in response to electrically stimulated afterdischarges was measured by RIA. ELH secretion from bag cells treated with protein-kinase inhibitors (Rp-cAMPS to inhibit PKA; H-7 to inhibit PKC) was compared to that from untreated controls. Our experiments showed that 100 μM Rp-cAMPS significantly attenuated ELH secretion during the nonbreeding seasons (winter and spring) of 2 consecutive years. This suggested a role for PKA in regulating ELH secretion. However, Rp-cAMPS had no effect on ELH secretion during the breeding seasons (summer and fall) of 2 consecutive years, even when the dose of Rp-cAMPS was increased to 200 μM. These findings indicate that there is a seasonal fluctuation in responsiveness to PKA inhibition. We also investigated if there was a seasonal fluctuation in the ability of the PKC inhibitor H-7 to suppress ELH secretion. During the nonbreeding season, 10–100 μM H-7 significantly inhibited ELH secretion, but during the breeding season, only the highest dose (100 μM) of H-7 inhibited ELH release. These results confirm that PKC plays a role in regulating ELH secretion and indicate that there is a seasonal fluctuation in responsiveness to PKC inhibition. Overall, our findings suggest that both the cAMP and diacylglycerol second-messenger pathways are regulated on a seasonal basis.</abstract><qualityIndicators><score>10</score><pdfWordCount>5690</pdfWordCount><pdfCharCount>35405</pdfCharCount><pdfVersion>1.1</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>554 x 734 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>290</abstractWordCount><abstractCharCount>1980</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of Aplysia californica to Inhibitors of Protein Kinase A and Protein Kinase C</title><pmid><json:string>9480743</json:string></pmid><pii><json:string>S0016-6480(97)97040-4</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>General and Comparative Endocrinology</title><language><json:string>unknown</json:string></language><publicationDate>1998</publicationDate><issn><json:string>0016-6480</json:string></issn><pii><json:string>S0016-6480(00)X0048-2</json:string></pii><volume>109</volume><issue>3</issue><pages><first>356</first><last>365</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1</json:string><json:string>1998</json:string><json:string>2</json:string></date><geogName></geogName><orgName><json:string>Grass Instruments</json:string><json:string>Hitachi Denshi, Ltd.</json:string><json:string>Yong-Montenegro TABLE</json:string><json:string>Sigma Chemical Co.</json:string><json:string>Baxter Healthcare Corp., Glendale</json:string><json:string>NIH Grants R29</json:string></orgName><orgName_funder><json:string>NIH Grants R29</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>J. Zool</json:string><json:string>Unless</json:string><json:string>Yun J. Kim</json:string><json:string>Christopher Colwell</json:string><json:string>Wenjau</json:string><json:string>Loechner</json:string><json:string>Summer Winter</json:string></persName><placeName><json:string>NY</json:string><json:string>Woodbury</json:string><json:string>Quincy</json:string><json:string>Montenegro</json:string><json:string>MA</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Strumwasser et al., 1969</json:string><json:string>Nagle et al., 1985</json:string><json:string>Audesirk, 1979</json:string><json:string>Driefuss et al., 1971</json:string><json:string>Wayne and Frumovitz, 1995</json:string><json:string>DeRiemer et al., 1985</json:string><json:string>Newcomb and Scheller, 1990</json:string><json:string>reviewed by Conn and Kaczmarek, 1989</json:string><json:string>Blankenship and Haskins, 1979</json:string><json:string>Berry, 1984</json:string><json:string>Kaczmarek et al., 1980</json:string><json:string>Strong et al., 1987</json:string><json:string>Pinchasi et al., 1985</json:string><json:string>Forscher et al., 1987</json:string><json:string>Azhderian et al., 1994</json:string><json:string>Kaczmarek et al., 1979</json:string><json:string>Loechner et al., 1992</json:string><json:string>Heller et al., 1980</json:string><json:string>Chiu et al., 1979</json:string><json:string>Rothermel and Botelho, 1988</json:string><json:string>Ghirardi et al., 1992</json:string><json:string>Kubokawa et al., 1994</json:string><json:string>Jackson et al., 1991</json:string><json:string>Bruehl and Berry, 1985</json:string><json:string>Arch et al., 1978</json:string><json:string>Berry et al., 1994</json:string><json:string>Conn et al., 1989a</json:string><json:string>Choate et al., 1993</json:string><json:string>DeReimer et al., 1985</json:string><json:string>Berry, 1982</json:string><json:string>Wayne and Block, 1992</json:string><json:string>Coggeshall, 1970</json:string><json:string>Conn et al., 1989a,b</json:string><json:string>Loechner et al., 1990</json:string><json:string>Bernheim and Mayeri, 1995</json:string><json:string>Jennings et al., 1982</json:string><json:string>Pinsker and Parsons, 1985</json:string><json:string>Arch and Berry, 1989</json:string><json:string>Conn and Kaczmarek, 1989</json:string><json:string>Wayne and Wong (1994)</json:string><json:string>Berry, 1986</json:string><json:string>Redman and Berry, 1991</json:string><json:string>Dudek and Kossatz, 1982</json:string><json:string>Wayne and Wong, 1994</json:string><json:string>Azhderian and Kaczmarek, 1990</json:string><json:string>Kaczmarek et al., 1978, 1980</json:string><json:string>Strong and Kaczmarek, 1986</json:string><json:string>Kupfermann and Kandel, 1970</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-1GW00B1C-X</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - endocrinology & metabolism</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - endocrinology & metabolism</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Endocrinology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1006/gcen.1997.7040</json:string></doi><id>39E36B3482B080ACFCD87AA71833CE43B5FC91F8</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/39E36B3482B080ACFCD87AA71833CE43B5FC91F8/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/39E36B3482B080ACFCD87AA71833CE43B5FC91F8/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/39E36B3482B080ACFCD87AA71833CE43B5FC91F8/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of Aplysia californica to Inhibitors of Protein Kinase A and Protein Kinase C</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1998 Academic Press</p></availability><date>1998</date></publicationStmt><notesStmt><note>Preliminary results from portions of this study were reported in the Proceedings of the Fourth Symposium on Molluscan Neurobiology (Netherlands J. Zool.44,588–598).</note><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of Aplysia californica to Inhibitors of Protein Kinase A and Protein Kinase C</title><author xml:id="author-0000"><persName><forename type="first">Nancy L.</forename><surname>Wayne</surname></persName><affiliation>To whom correspondence should be addressed.</affiliation><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Yun J.</forename><surname>Kim</surname></persName><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Rocio J.</forename><surname>Yong-Montenegro</surname></persName><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation></author><idno type="istex">39E36B3482B080ACFCD87AA71833CE43B5FC91F8</idno><idno type="DOI">10.1006/gcen.1997.7040</idno><idno type="PII">S0016-6480(97)97040-4</idno></analytic><monogr><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="pISSN">0016-6480</idno><idno type="PII">S0016-6480(00)X0048-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">109</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="356">356</biblScope><biblScope unit="page" to="365">365</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The neuroendocrine bag cells ofAplysiaprovide an excellent model system for exploring the roles of second-messenger pathways regulating peptide hormone secretion. Both the cAMP and diacylglycerol second-messenger systems and their associated protein kinases (PKA and PKC) are involved in regulating membrane excitability in bag-cell neurons ofAplysia.The purpose of the present set of experiments was to determine if PKA and PKC also play roles in regulating egg laying hormone (ELH) secretion from bag-cell neurons. Abdominal ganglia with attached bag-cell clusters and connective nerves were dissected from reproductively matureAplysia,and ELH secretion in response to electrically stimulated afterdischarges was measured by RIA. ELH secretion from bag cells treated with protein-kinase inhibitors (Rp-cAMPS to inhibit PKA; H-7 to inhibit PKC) was compared to that from untreated controls. Our experiments showed that 100 μM Rp-cAMPS significantly attenuated ELH secretion during the nonbreeding seasons (winter and spring) of 2 consecutive years. This suggested a role for PKA in regulating ELH secretion. However, Rp-cAMPS had no effect on ELH secretion during the breeding seasons (summer and fall) of 2 consecutive years, even when the dose of Rp-cAMPS was increased to 200 μM. These findings indicate that there is a seasonal fluctuation in responsiveness to PKA inhibition. We also investigated if there was a seasonal fluctuation in the ability of the PKC inhibitor H-7 to suppress ELH secretion. During the nonbreeding season, 10–100 μM H-7 significantly inhibited ELH secretion, but during the breeding season, only the highest dose (100 μM) of H-7 inhibited ELH release. These results confirm that PKC plays a role in regulating ELH secretion and indicate that there is a seasonal fluctuation in responsiveness to PKC inhibition. Overall, our findings suggest that both the cAMP and diacylglycerol second-messenger pathways are regulated on a seasonal basis.</p></abstract></profileDesc><revisionDesc><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/39E36B3482B080ACFCD87AA71833CE43B5FC91F8/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YGCEN</jid><aid>97040</aid><ce:pii>S0016-6480(97)97040-4</ce:pii><ce:doi>10.1006/gcen.1997.7040</ce:doi><ce:copyright type="full-transfer" year="1998">Academic Press</ce:copyright></item-info><head><ce:article-footnote><ce:label>☆</ce:label><ce:note-para>Preliminary results from portions of this study were reported in the Proceedings of the Fourth Symposium on Molluscan Neurobiology (<ce:italic>Netherlands J. Zool.</ce:italic><ce:bold>44,</ce:bold>588–598).</ce:note-para></ce:article-footnote><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of<ce:italic>Aplysia californica</ce:italic>to Inhibitors of Protein Kinase A and Protein Kinase C</ce:title><ce:author-group><ce:author><ce:given-name>Nancy L.</ce:given-name><ce:surname>Wayne</ce:surname><ce:cross-ref refid="GC977040FN2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Yun J.</ce:given-name><ce:surname>Kim</ce:surname></ce:author><ce:author><ce:given-name>Rocio J.</ce:given-name><ce:surname>Yong-Montenegro</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</ce:textfn></ce:affiliation><ce:footnote id="GC977040FN2"><ce:label>2</ce:label><ce:note-para>To whom correspondence should be addressed.</ce:note-para></ce:footnote></ce:author-group><ce:date-accepted day="17" month="11" year="1997"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The neuroendocrine bag cells of<ce:italic>Aplysia</ce:italic>provide an excellent model system for exploring the roles of second-messenger pathways regulating peptide hormone secretion. Both the cAMP and diacylglycerol second-messenger systems and their associated protein kinases (PKA and PKC) are involved in regulating membrane excitability in bag-cell neurons of<ce:italic>Aplysia.</ce:italic>The purpose of the present set of experiments was to determine if PKA and PKC also play roles in regulating egg laying hormone (ELH) secretion from bag-cell neurons. Abdominal ganglia with attached bag-cell clusters and connective nerves were dissected from reproductively mature<ce:italic>Aplysia,</ce:italic>and ELH secretion in response to electrically stimulated afterdischarges was measured by RIA. ELH secretion from bag cells treated with protein-kinase inhibitors (Rp-cAMPS to inhibit PKA; H-7 to inhibit PKC) was compared to that from untreated controls. Our experiments showed that 100 μM Rp-cAMPS significantly attenuated ELH secretion during the nonbreeding seasons (winter and spring) of 2 consecutive years. This suggested a role for PKA in regulating ELH secretion. However, Rp-cAMPS had no effect on ELH secretion during the breeding seasons (summer and fall) of 2 consecutive years, even when the dose of Rp-cAMPS was increased to 200 μM. These findings indicate that there is a seasonal fluctuation in responsiveness to PKA inhibition. We also investigated if there was a seasonal fluctuation in the ability of the PKC inhibitor H-7 to suppress ELH secretion. During the nonbreeding season, 10–100 μM H-7 significantly inhibited ELH secretion, but during the breeding season, only the highest dose (100 μM) of H-7 inhibited ELH release. These results confirm that PKC plays a role in regulating ELH secretion and indicate that there is a seasonal fluctuation in responsiveness to PKC inhibition. Overall, our findings suggest that both the cAMP and diacylglycerol second-messenger pathways are regulated on a seasonal basis.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of Aplysia californica to Inhibitors of Protein Kinase A and Protein Kinase C</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells of</title></titleInfo><name type="personal"><namePart type="given">Nancy L.</namePart><namePart type="family">Wayne</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation><description>To whom correspondence should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yun J.</namePart><namePart type="family">Kim</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rocio J.</namePart><namePart type="family">Yong-Montenegro</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The neuroendocrine bag cells ofAplysiaprovide an excellent model system for exploring the roles of second-messenger pathways regulating peptide hormone secretion. Both the cAMP and diacylglycerol second-messenger systems and their associated protein kinases (PKA and PKC) are involved in regulating membrane excitability in bag-cell neurons ofAplysia.The purpose of the present set of experiments was to determine if PKA and PKC also play roles in regulating egg laying hormone (ELH) secretion from bag-cell neurons. Abdominal ganglia with attached bag-cell clusters and connective nerves were dissected from reproductively matureAplysia,and ELH secretion in response to electrically stimulated afterdischarges was measured by RIA. ELH secretion from bag cells treated with protein-kinase inhibitors (Rp-cAMPS to inhibit PKA; H-7 to inhibit PKC) was compared to that from untreated controls. Our experiments showed that 100 μM Rp-cAMPS significantly attenuated ELH secretion during the nonbreeding seasons (winter and spring) of 2 consecutive years. This suggested a role for PKA in regulating ELH secretion. However, Rp-cAMPS had no effect on ELH secretion during the breeding seasons (summer and fall) of 2 consecutive years, even when the dose of Rp-cAMPS was increased to 200 μM. These findings indicate that there is a seasonal fluctuation in responsiveness to PKA inhibition. We also investigated if there was a seasonal fluctuation in the ability of the PKC inhibitor H-7 to suppress ELH secretion. During the nonbreeding season, 10–100 μM H-7 significantly inhibited ELH secretion, but during the breeding season, only the highest dose (100 μM) of H-7 inhibited ELH release. These results confirm that PKC plays a role in regulating ELH secretion and indicate that there is a seasonal fluctuation in responsiveness to PKC inhibition. Overall, our findings suggest that both the cAMP and diacylglycerol second-messenger pathways are regulated on a seasonal basis.</abstract><note>Preliminary results from portions of this study were reported in the Proceedings of the Fourth Symposium on Molluscan Neurobiology (Netherlands J. Zool.44,588–598).</note><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>General and Comparative Endocrinology</title></titleInfo><titleInfo type="abbreviated"><title>YGCEN</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">199803</dateIssued></originInfo><identifier type="ISSN">0016-6480</identifier><identifier type="PII">S0016-6480(00)X0048-2</identifier><part><date>199803</date><detail type="volume"><number>109</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>293</start><end>392</end></extent><extent unit="pages"><start>356</start><end>365</end></extent></part></relatedItem><identifier type="istex">39E36B3482B080ACFCD87AA71833CE43B5FC91F8</identifier><identifier type="ark">ark:/67375/6H6-1GW00B1C-X</identifier><identifier type="DOI">10.1006/gcen.1997.7040</identifier><identifier type="PII">S0016-6480(97)97040-4</identifier><accessCondition type="use and reproduction" contentType="copyright">©1998 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1998</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/39E36B3482B080ACFCD87AA71833CE43B5FC91F8/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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