The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia
Identifieur interne : 000552 ( Istex/Corpus ); précédent : 000551; suivant : 000553The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia
Auteurs : Wenjau Lee ; Nancy L. WayneSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1997.
English descriptors
- Teeft :
- Abdominal ganglia, Abdominal ganglion, Academic press, Afterdischarge, Anisomycin, Anisomycin treatment, Aplysia, Aplysia californica, Californica, Cell activities, Cell afterdischarge, Cell cluster, Cell clusters, Cell content, Cell control, Cell homogenates, Cell neurons, Cell preparation, Cell preparations, Cell system, Cellular regulation, Compound action potentials, Consecutive days, Control period, Control sample, Copyright, Culture medium, Daily afterdischarges, Daily stimulation, Degradation, Electrical afterdischarge, Electrical stimulation, Endocrinology, Experimental design, Experimental period, Experimental sample, Ganglion, Glucose, Growth hormone, High rate, Hormone secretion, Hormone synthesis, Inhibitor, Intermediate products, Kaczmarek, Linear regression analysis, Lower values, Major role, Membrane excitability, Neuroendocrine, Neuroendocrine cells, Neuron, Other cluster, Other hand, Overall pattern, Pancreatic beta cells, Parathyroid hormone, Peptide, Percentage release, Physiol, Preferential release, Present study, Prohormone, Protein synthesis, Protein synthesis inhibitor, Radiolabeled, Relative contribution, Releasable, Room temperature, Same concentration, Sample collection, Scheller, Secretion, Secretory, Secretory granules, Secretory material, Successive days, Suction electrode, Sympathetic ganglion, Total amount, Total amounts, Ucla school.
Abstract
Abstract: The neuroendocrine bag cells fromAplysiacontrol reproductive functions through the regulated release of egg-laying hormone (ELH). The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [3H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying inAplysia,indicating that the contribution of newly synthesized ELH was physiologically relevant.
Url:
DOI: 10.1006/gcen.1997.6916
Links to Exploration step
ISTEX:A050ECB5D9A6340A34E7F23EC4A86C36E3566347Le document en format XML
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Wayne</name><affiliation><mods:affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="ISSN">0016-6480</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">107</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="201">201</biblScope><biblScope unit="page" to="211">211</biblScope></imprint><idno type="ISSN">0016-6480</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0016-6480</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Abdominal ganglia</term><term>Abdominal ganglion</term><term>Academic press</term><term>Afterdischarge</term><term>Anisomycin</term><term>Anisomycin treatment</term><term>Aplysia</term><term>Aplysia californica</term><term>Californica</term><term>Cell activities</term><term>Cell afterdischarge</term><term>Cell cluster</term><term>Cell clusters</term><term>Cell content</term><term>Cell control</term><term>Cell homogenates</term><term>Cell neurons</term><term>Cell preparation</term><term>Cell preparations</term><term>Cell system</term><term>Cellular regulation</term><term>Compound action potentials</term><term>Consecutive days</term><term>Control period</term><term>Control sample</term><term>Copyright</term><term>Culture medium</term><term>Daily afterdischarges</term><term>Daily stimulation</term><term>Degradation</term><term>Electrical afterdischarge</term><term>Electrical stimulation</term><term>Endocrinology</term><term>Experimental design</term><term>Experimental period</term><term>Experimental sample</term><term>Ganglion</term><term>Glucose</term><term>Growth hormone</term><term>High rate</term><term>Hormone secretion</term><term>Hormone synthesis</term><term>Inhibitor</term><term>Intermediate products</term><term>Kaczmarek</term><term>Linear regression analysis</term><term>Lower values</term><term>Major role</term><term>Membrane excitability</term><term>Neuroendocrine</term><term>Neuroendocrine cells</term><term>Neuron</term><term>Other cluster</term><term>Other hand</term><term>Overall pattern</term><term>Pancreatic beta cells</term><term>Parathyroid hormone</term><term>Peptide</term><term>Percentage release</term><term>Physiol</term><term>Preferential release</term><term>Present study</term><term>Prohormone</term><term>Protein synthesis</term><term>Protein synthesis inhibitor</term><term>Radiolabeled</term><term>Relative contribution</term><term>Releasable</term><term>Room temperature</term><term>Same concentration</term><term>Sample collection</term><term>Scheller</term><term>Secretion</term><term>Secretory</term><term>Secretory granules</term><term>Secretory material</term><term>Successive days</term><term>Suction electrode</term><term>Sympathetic ganglion</term><term>Total amount</term><term>Total amounts</term><term>Ucla school</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The neuroendocrine bag cells fromAplysiacontrol reproductive functions through the regulated release of egg-laying hormone (ELH). The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [3H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying inAplysia,indicating that the contribution of newly synthesized ELH was physiologically relevant.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>aplysia</json:string><json:string>afterdischarge</json:string><json:string>anisomycin</json:string><json:string>cell clusters</json:string><json:string>neuroendocrine</json:string><json:string>secretory</json:string><json:string>ganglion</json:string><json:string>releasable</json:string><json:string>copyright</json:string><json:string>academic press</json:string><json:string>scheller</json:string><json:string>californica</json:string><json:string>electrical stimulation</json:string><json:string>endocrinology</json:string><json:string>protein synthesis</json:string><json:string>peptide</json:string><json:string>experimental period</json:string><json:string>kaczmarek</json:string><json:string>abdominal ganglion</json:string><json:string>compound action potentials</json:string><json:string>glucose</json:string><json:string>radiolabeled</json:string><json:string>physiol</json:string><json:string>aplysia californica</json:string><json:string>prohormone</json:string><json:string>neuron</json:string><json:string>daily stimulation</json:string><json:string>cell neurons</json:string><json:string>room temperature</json:string><json:string>hormone synthesis</json:string><json:string>experimental design</json:string><json:string>cell preparations</json:string><json:string>protein synthesis inhibitor</json:string><json:string>present study</json:string><json:string>consecutive days</json:string><json:string>secretion</json:string><json:string>degradation</json:string><json:string>control period</json:string><json:string>secretory material</json:string><json:string>cell content</json:string><json:string>percentage release</json:string><json:string>other hand</json:string><json:string>neuroendocrine cells</json:string><json:string>abdominal ganglia</json:string><json:string>total amount</json:string><json:string>other cluster</json:string><json:string>culture medium</json:string><json:string>membrane excitability</json:string><json:string>growth hormone</json:string><json:string>secretory granules</json:string><json:string>inhibitor</json:string><json:string>suction electrode</json:string><json:string>successive days</json:string><json:string>high rate</json:string><json:string>same concentration</json:string><json:string>sample collection</json:string><json:string>major role</json:string><json:string>cell activities</json:string><json:string>control sample</json:string><json:string>overall pattern</json:string><json:string>cell homogenates</json:string><json:string>linear regression analysis</json:string><json:string>cell afterdischarge</json:string><json:string>relative contribution</json:string><json:string>experimental sample</json:string><json:string>daily afterdischarges</json:string><json:string>cell preparation</json:string><json:string>intermediate products</json:string><json:string>electrical afterdischarge</json:string><json:string>cellular regulation</json:string><json:string>anisomycin treatment</json:string><json:string>pancreatic beta cells</json:string><json:string>lower values</json:string><json:string>cell cluster</json:string><json:string>total amounts</json:string><json:string>parathyroid hormone</json:string><json:string>ucla school</json:string><json:string>cell system</json:string><json:string>cell control</json:string><json:string>preferential release</json:string><json:string>sympathetic ganglion</json:string><json:string>hormone secretion</json:string></teeft></keywords><author><json:item><name>Wenjau Lee</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</json:string></affiliations></json:item><json:item><name>Nancy L. Wayne</name><affiliations><json:string>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-W92B1X4G-T</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The neuroendocrine bag cells fromAplysiacontrol reproductive functions through the regulated release of egg-laying hormone (ELH). The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [3H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying inAplysia,indicating that the contribution of newly synthesized ELH was physiologically relevant.</abstract><qualityIndicators><score>9.916</score><pdfWordCount>6714</pdfWordCount><pdfCharCount>40249</pdfCharCount><pdfVersion>1.1</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>554 x 734 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>243</abstractWordCount><abstractCharCount>1591</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia</title><pmid><json:string>9245528</json:string></pmid><pii><json:string>S0016-6480(97)96916-1</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>General and Comparative Endocrinology</title><language><json:string>unknown</json:string></language><publicationDate>1997</publicationDate><issn><json:string>0016-6480</json:string></issn><pii><json:string>S0016-6480(00)X0056-1</json:string></pii><volume>107</volume><issue>2</issue><pages><first>201</first><last>211</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1997</json:string><json:string>35S</json:string></date><geogName></geogName><orgName><json:string>National Diagnostics, Atlanta, GA</json:string><json:string>Department of Physiology</json:string><json:string>Peninsula Laboratories</json:string><json:string>Sigma Chemical Co.</json:string><json:string>Department of Physiology, UCLA School of Medicine, Room</json:string><json:string>HD</json:string><json:string>NIH</json:string></orgName><orgName_funder><json:string>HD</json:string><json:string>NIH</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Nancy L. Wayne</json:string><json:string>Wayne Beach</json:string><json:string>Yun Jin</json:string><json:string>Costa Mesa</json:string><json:string>Unless</json:string></persName><placeName><json:string>IL</json:string><json:string>Rochester</json:string><json:string>NY</json:string><json:string>Belmont</json:string><json:string>CA</json:string><json:string>Arlington Heights</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Basch and Talamantes, 1986</json:string><json:string>Loh et al., 1975</json:string><json:string>Halban, 1982</json:string><json:string>Jung and Scheller, 1991</json:string><json:string>Luz et al., 1983, 1985</json:string><json:string>Nagle et al., 1985</json:string><json:string>Hanley and Wellings, 1985</json:string><json:string>Newcomb and Scheller, 1990</json:string><json:string>Blankenship and Haskins, 1979</json:string><json:string>Wayne et al., 1996</json:string><json:string>Berry and Yates, 1986</json:string><json:string>Chu et al. (1983)</json:string><json:string>Kaczmarek et al., 1979</json:string><json:string>Chiu et al., 1979</json:string><json:string>Sossin et al., 1990</json:string><json:string>Stachura et al., 1989</json:string><json:string>Pinsker and Dudek, 1977</json:string><json:string>Molenaar and Polak, 1976</json:string><json:string>ELH; Kupfermann, 1970</json:string><json:string>Mangat and De Bold, 1993</json:string><json:string>Wayne and Block, 1992</json:string><json:string>Coggeshall, 1970</json:string><json:string>Maas et al., 1991</json:string><json:string>Rothman et al., 1983</json:string><json:string>Prado et al., 1992</json:string><json:string>Loechner et al., 1990</json:string><json:string>Bernheim and Mayeri, 1995</json:string><json:string>Pinsker and Parsons, 1985</json:string><json:string>Fisher et al., 1988</json:string><json:string>Arch and Berry, 1989</json:string><json:string>Conn and Kaczmarek, 1989</json:string><json:string>Laemmli, 1970</json:string><json:string>Halban (1982)</json:string><json:string>Wayne and Wong, 1994</json:string><json:string>Azhderian and Kaczmarek, 1990</json:string><json:string>Arch et al., 1976</json:string><json:string>Schwartz et al., 1971</json:string><json:string>Collier, 1969</json:string><json:string>Piercy and Shin, 1981</json:string><json:string>Scheller et al., 1983</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-W92B1X4G-T</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - endocrinology & metabolism</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - endocrinology & metabolism</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Endocrinology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1997</publicationDate><copyrightDate>1997</copyrightDate><doi><json:string>10.1006/gcen.1997.6916</json:string></doi><id>A050ECB5D9A6340A34E7F23EC4A86C36E3566347</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/A050ECB5D9A6340A34E7F23EC4A86C36E3566347/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/A050ECB5D9A6340A34E7F23EC4A86C36E3566347/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/A050ECB5D9A6340A34E7F23EC4A86C36E3566347/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1997 Academic Press</p></availability><date>1997</date></publicationStmt><notesStmt><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia</title><author xml:id="author-0000"><persName><forename type="first">Wenjau</forename><surname>Lee</surname></persName><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Nancy L.</forename><surname>Wayne</surname></persName><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation></author><idno type="istex">A050ECB5D9A6340A34E7F23EC4A86C36E3566347</idno><idno type="DOI">10.1006/gcen.1997.6916</idno><idno type="PII">S0016-6480(97)96916-1</idno></analytic><monogr><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="pISSN">0016-6480</idno><idno type="PII">S0016-6480(00)X0056-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997"></date><biblScope unit="volume">107</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="201">201</biblScope><biblScope unit="page" to="211">211</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1997</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The neuroendocrine bag cells fromAplysiacontrol reproductive functions through the regulated release of egg-laying hormone (ELH). The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [3H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying inAplysia,indicating that the contribution of newly synthesized ELH was physiologically relevant.</p></abstract></profileDesc><revisionDesc><change when="1997">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/A050ECB5D9A6340A34E7F23EC4A86C36E3566347/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YGCEN</jid><aid>96916</aid><ce:pii>S0016-6480(97)96916-1</ce:pii><ce:doi>10.1006/gcen.1997.6916</ce:doi><ce:copyright type="full-transfer" year="1997">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of<ce:italic>Aplysia</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>Wenjau</ce:given-name><ce:surname>Lee</ce:surname></ce:author><ce:author><ce:given-name>Nancy L.</ce:given-name><ce:surname>Wayne</ce:surname><ce:cross-ref refid="GC976916FN1">1<ce:footnote id="GC976916FN1"><ce:label>1</ce:label><ce:note-para>To whom correspondence should be addressed at Department of Physiology, UCLA School of Medicine, Room 53-231 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095-1751. Fax: (310) 206-5661. E-mail: nwayne@physiology.medsch.ucla.edu.</ce:note-para></ce:footnote></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</ce:textfn></ce:affiliation></ce:author-group><ce:date-accepted day="17" month="3" year="1997"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The neuroendocrine bag cells from<ce:italic>Aplysia</ce:italic>control reproductive functions through the regulated release of egg-laying hormone (ELH). The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [<ce:sup>3</ce:sup>H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying in<ce:italic>Aplysia,</ce:italic>indicating that the contribution of newly synthesized ELH was physiologically relevant.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of Aplysia</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>The Fate of Newly Synthesized Hormone from Neuroendocrine Cells of</title></titleInfo><name type="personal"><namePart type="given">Wenjau</namePart><namePart type="family">Lee</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nancy L.</namePart><namePart type="family">Wayne</namePart><affiliation>Department of Physiology, UCLA School of Medicine, Los Angeles, California, 90095-1751</affiliation><description>To whom correspondence should be addressed at Department of Physiology, UCLA School of Medicine, Room 53-231 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095-1751. Fax: (310) 206-5661. E-mail: nwayne@physiology.medsch.ucla.edu.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1997</dateIssued><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The neuroendocrine bag cells fromAplysiacontrol reproductive functions through the regulated release of egg-laying hormone (ELH). The purposes of this study were to investigate (1) if synthesis of ELH in bag cells compensates for the release and degradation of the hormone, (2) the ratio of released to stored ELH, (3) the fate of newly synthesized ELH and its relative contribution to the overall pattern of ELH secretion, and (4) the consequences of blocking protein synthesis in bag cells on the pattern of ELH secretion and subsequent reproductive function. Excised bag cells were incubated with [3H]leucine to radiolabel newly synthesized ELH and were then electrically stimulated to trigger secretion of the hormone on 4 successive days, mimicking bag cell activities during the breeding season. Samples of bag cell secretion were collected; total (new and old) and radiolabeled (new) ELH levels were determined. The results showed that ELH synthesis could not keep up with the daily loss of ELH through release and degradation, but the ratio of released to stored ELH remained constant over the 4-day study. In addition, releasable ELH was secreted preferentially within 24 hr after it was synthesized. Blocking protein synthesis resulted in a 50% decrease in total ELH released, suggesting that newly synthesized ELH contributes to half of the total ELH secreted. Furthermore, this lowered level of total ELH, i.e., older ELH alone, was not sufficient to stimulate egg laying inAplysia,indicating that the contribution of newly synthesized ELH was physiologically relevant.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>General and Comparative Endocrinology</title></titleInfo><titleInfo type="abbreviated"><title>YGCEN</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">199708</dateIssued></originInfo><identifier type="ISSN">0016-6480</identifier><identifier type="PII">S0016-6480(00)X0056-1</identifier><part><date>199708</date><detail type="volume"><number>107</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>147</start><end>290</end></extent><extent unit="pages"><start>201</start><end>211</end></extent></part></relatedItem><identifier type="istex">A050ECB5D9A6340A34E7F23EC4A86C36E3566347</identifier><identifier type="ark">ark:/67375/6H6-W92B1X4G-T</identifier><identifier type="DOI">10.1006/gcen.1997.6916</identifier><identifier type="PII">S0016-6480(97)96916-1</identifier><accessCondition type="use and reproduction" contentType="copyright">©1997 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1997</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/A050ECB5D9A6340A34E7F23EC4A86C36E3566347/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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