Shaping tubes in cells
Identifieur interne : 000161 ( Hal/Checkpoint ); précédent : 000160; suivant : 000162Shaping tubes in cells
Auteurs : Martin Lenz [France]Source :
Descripteurs français
English descriptors
Abstract
Membrane remodeling events are essential for the life of the cell, and involve membrane tubes shaped by proteins. We study three different configurations where such tubes appear. We first focus on the helical dynamin polymer, which encircles membrane tubes and severs them upon GTP hydrolysis. Dynamin recruitment is shown to depend on the membrane's curvature. We formulate hypotheses and propose experiments to understand the nucleation of the dynamin polymer and its interactions with the membrane. Dynamin's GTP-induced concerted conformational change is described using generalized hydrodynamics and seemingly contradictory experimental results are reconciled through mechanical arguments. The long-time dynamics of the dynamin-membrane tube is diffusive and dominated by an effective dynamin/membrane friction, which experiments confirm. Our second topic is the ESCRT-III complex, which tubulates flat membranes and assembles inside of them. We account for this deformation with a novel buckling instability arising when sticky curved filaments bind to the membrane. This hypothesis could be verified experimentally. A metastable regime for the flat membrane is uncovered, which the cell could use to quickly generate tubes. Thirdly, we turn to stereocilia, which are actin-based cellular protrusions essential for hearing. We predict their shape from the detachment dynamics of actin cross-linkers, which accounts for experimental data. If the cross-linkers are allowed to reattach, our model yields a dynamical phase transition towards unbounded growth and numerical simulations suggest an anomalous power-law divergence of the protrusion length.
Url:
Links toward previous steps (curation, corpus...)
Links to Exploration step
Hal:tel-00541655Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Shaping tubes in cells</title><author><name sortKey="Lenz, Martin" sort="Lenz, Martin" uniqKey="Lenz M" first="Martin" last="Lenz">Martin Lenz</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-509" status="VALID"> <idno type="RNSR">199411684F</idno> <orgName>Physico-Chimie-Curie</orgName> <orgName type="acronym">PCC</orgName> <desc> <address> <addrLine>Bâtiment Curie 26 rue d'Ulm 75248 PARIS CEDEX 05</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.upmc.fr/fr/recherche/pole_2/pole_energie_matiere_et_univers2/physico_chimie_curie_pcc_umr_168.html</ref> </desc> <listRelation> <relation active="#struct-93591" type="direct"></relation> <relation active="#struct-364868" type="direct"></relation> <relation name="UMR168" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-93591" type="direct"><org type="institution" xml:id="struct-93591" status="VALID"> <orgName>Université Pierre et Marie Curie - Paris 6</orgName> <orgName type="acronym">UPMC</orgName> <desc> <address> <addrLine>4 place Jussieu - 75005 Paris</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.upmc.fr/</ref> </desc> </org></tutelle><tutelle active="#struct-364868" type="direct"><org type="institution" xml:id="struct-364868" status="INCOMING"> <orgName>INSTITUT CURIE</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR168" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:tel-00541655</idno><idno type="halId">tel-00541655</idno><idno type="halUri">https://tel.archives-ouvertes.fr/tel-00541655</idno><idno type="url">https://tel.archives-ouvertes.fr/tel-00541655</idno><date when="2009-10-13">2009-10-13</date><idno type="wicri:Area/Hal/Corpus">000134</idno><idno type="wicri:Area/Hal/Curation">000134</idno><idno type="wicri:Area/Hal/Checkpoint">000161</idno><idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000161</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Shaping tubes in cells</title><author><name sortKey="Lenz, Martin" sort="Lenz, Martin" uniqKey="Lenz M" first="Martin" last="Lenz">Martin Lenz</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-509" status="VALID"> <idno type="RNSR">199411684F</idno> <orgName>Physico-Chimie-Curie</orgName> <orgName type="acronym">PCC</orgName> <desc> <address> <addrLine>Bâtiment Curie 26 rue d'Ulm 75248 PARIS CEDEX 05</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.upmc.fr/fr/recherche/pole_2/pole_energie_matiere_et_univers2/physico_chimie_curie_pcc_umr_168.html</ref> </desc> <listRelation> <relation active="#struct-93591" type="direct"></relation> <relation active="#struct-364868" type="direct"></relation> <relation name="UMR168" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-93591" type="direct"><org type="institution" xml:id="struct-93591" status="VALID"> <orgName>Université Pierre et Marie Curie - Paris 6</orgName> <orgName type="acronym">UPMC</orgName> <desc> <address> <addrLine>4 place Jussieu - 75005 Paris</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.upmc.fr/</ref> </desc> </org></tutelle><tutelle active="#struct-364868" type="direct"><org type="institution" xml:id="struct-364868" status="INCOMING"> <orgName>INSTITUT CURIE</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR168" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author></analytic></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>biophysics</term><term>dynamin</term><term>out-of-equilibrium</term><term>protein-membrane interactions</term><term>stereocilia</term></keywords><keywords scheme="mix" xml:lang="fr"><term>ESCRT-III</term><term>biophysique</term><term>dynamine</term><term>hors équilibre</term><term>interactions protéines-membrane</term><term>stéréocils</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Membrane remodeling events are essential for the life of the cell, and involve membrane tubes shaped by proteins. We study three different configurations where such tubes appear. We first focus on the helical dynamin polymer, which encircles membrane tubes and severs them upon GTP hydrolysis. Dynamin recruitment is shown to depend on the membrane's curvature. We formulate hypotheses and propose experiments to understand the nucleation of the dynamin polymer and its interactions with the membrane. Dynamin's GTP-induced concerted conformational change is described using generalized hydrodynamics and seemingly contradictory experimental results are reconciled through mechanical arguments. The long-time dynamics of the dynamin-membrane tube is diffusive and dominated by an effective dynamin/membrane friction, which experiments confirm. Our second topic is the ESCRT-III complex, which tubulates flat membranes and assembles inside of them. We account for this deformation with a novel buckling instability arising when sticky curved filaments bind to the membrane. This hypothesis could be verified experimentally. A metastable regime for the flat membrane is uncovered, which the cell could use to quickly generate tubes. Thirdly, we turn to stereocilia, which are actin-based cellular protrusions essential for hearing. We predict their shape from the detachment dynamics of actin cross-linkers, which accounts for experimental data. If the cross-linkers are allowed to reattach, our model yields a dynamical phase transition towards unbounded growth and numerical simulations suggest an anomalous power-law divergence of the protrusion length.</div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Shaping tubes in cells</title> <author role="aut"> <persName> <forename type="first">Martin</forename> <surname>Lenz</surname> </persName> <email type="md5">6055d4f9bdc64fb0149eefc721d350e6</email> <email type="domain">uchicago.edu</email> <idno type="halauthorid">552968</idno> <affiliation ref="#struct-509"></affiliation> </author> <editor role="depositor"> <persName> <forename>Martin</forename> <surname>Lenz</surname> </persName> <email type="md5">6055d4f9bdc64fb0149eefc721d350e6</email> <email type="domain">uchicago.edu</email> </editor> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2010-11-30 23:38:10</date> <date type="whenModified">2018-01-11 06:15:14</date> <date type="whenReleased">2010-12-02 14:42:35</date> <date type="whenProduced">2009-10-13</date> <date type="whenEndEmbargoed">2010-11-30</date> <ref type="file" target="https://tel.archives-ouvertes.fr/tel-00541655/document"> <date notBefore="2010-11-30"></date> </ref> <ref type="file" n="1" target="https://tel.archives-ouvertes.fr/tel-00541655/file/Lenz2009.pdf"> <date notBefore="2010-11-30"></date> </ref> </edition> <respStmt> <resp>contributor</resp> <name key="156878"> <persName> <forename>Martin</forename> <surname>Lenz</surname> </persName> <email type="md5">6055d4f9bdc64fb0149eefc721d350e6</email> <email type="domain">uchicago.edu</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">tel-00541655</idno> <idno type="halUri">https://tel.archives-ouvertes.fr/tel-00541655</idno> <idno type="halBibtex">lenz:tel-00541655</idno> <idno type="halRefHtml">Biological Physics [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2009. English</idno> <idno type="halRef">Biological Physics [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2009. English</idno> </publicationStmt> <seriesStmt> <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno> <idno type="stamp" n="THESES-UPMC">Thèses de l'Université Pierre et Marie Curie</idno> <idno type="stamp" n="FNCLCC" p="INSERM">UNICANCER</idno> <idno type="stamp" n="PCC_UMR168" p="UPMC">Physico-Chimie Curie</idno> <idno type="stamp" n="UPMC">Université Pierre et Marie Curie</idno> <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno> <idno type="stamp" n="PSL">Paris Sciences et Lettres (PSL) Research University</idno> <idno type="stamp" n="CURIE" p="FNCLCC">Institut Curie</idno> <idno type="stamp" n="UPMC_POLE_2" p="UPMC">UPMC Pôle 2</idno> </seriesStmt> <notesStmt></notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Shaping tubes in cells</title> <author role="aut"> <persName> <forename type="first">Martin</forename> <surname>Lenz</surname> </persName> <email type="md5">6055d4f9bdc64fb0149eefc721d350e6</email> <email type="domain">uchicago.edu</email> <idno type="halauthorid">552968</idno> <affiliation ref="#struct-509"></affiliation> </author> </analytic> <monogr> <imprint> <date type="dateDefended">2009-10-13</date> </imprint> <authority type="institution">Université Pierre et Marie Curie - Paris VI</authority> <authority type="supervisor">Jean-François Joanny & Jacques Prost</authority> <authority type="jury">Jean-François Joanny (directeur)</authority> <authority type="jury">Jacques Prost (co-directeur)</authority> <authority type="jury">Luca Peliti (rapporteur)</authority> <authority type="jury">Samuel A. Safran (rapporteur)</authority> <authority type="jury">Alain Comtet (examinateur)</authority> <authority type="jury">François Gallet (président)</authority> </monogr> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <keywords scheme="author"> <term xml:lang="en">biophysics</term> <term xml:lang="en">protein-membrane interactions</term> <term xml:lang="en">dynamin</term> <term xml:lang="en">stereocilia</term> <term xml:lang="en">out-of-equilibrium</term> <term xml:lang="fr">biophysique</term> <term xml:lang="fr">interactions protéines-membrane</term> <term xml:lang="fr">dynamine</term> <term xml:lang="fr">ESCRT-III</term> <term xml:lang="fr">stéréocils</term> <term xml:lang="fr">hors équilibre</term> </keywords> <classCode scheme="halDomain" n="phys.phys.phys-bio-ph">Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]</classCode> <classCode scheme="halTypology" n="THESE">Theses</classCode> </textClass> <abstract xml:lang="en">Membrane remodeling events are essential for the life of the cell, and involve membrane tubes shaped by proteins. We study three different configurations where such tubes appear. We first focus on the helical dynamin polymer, which encircles membrane tubes and severs them upon GTP hydrolysis. Dynamin recruitment is shown to depend on the membrane's curvature. We formulate hypotheses and propose experiments to understand the nucleation of the dynamin polymer and its interactions with the membrane. Dynamin's GTP-induced concerted conformational change is described using generalized hydrodynamics and seemingly contradictory experimental results are reconciled through mechanical arguments. The long-time dynamics of the dynamin-membrane tube is diffusive and dominated by an effective dynamin/membrane friction, which experiments confirm. Our second topic is the ESCRT-III complex, which tubulates flat membranes and assembles inside of them. We account for this deformation with a novel buckling instability arising when sticky curved filaments bind to the membrane. This hypothesis could be verified experimentally. A metastable regime for the flat membrane is uncovered, which the cell could use to quickly generate tubes. Thirdly, we turn to stereocilia, which are actin-based cellular protrusions essential for hearing. We predict their shape from the detachment dynamics of actin cross-linkers, which accounts for experimental data. If the cross-linkers are allowed to reattach, our model yields a dynamical phase transition towards unbounded growth and numerical simulations suggest an anomalous power-law divergence of the protrusion length.</abstract> <abstract xml:lang="fr">La cellule remodèle sa membrane en permanence, ce qui entraîne la formation de tubes de membrane façonnés par des protéines. Nous étudions trois cas impliquant de tels tubes. Le premier est le polymère hélicoïdal de dynamine, qui enveloppe les tubes de membrane puis les coupe en hydrolysant le GTP. Nous montrons que le recrutement de la dynamine dépend de la courbure de la membrane. Nous formulons des hypothèses et proposons des expériences pour comprendre la nucléation du polymère de dynamine et ses interactions avec la membrane. Nous donnons une description hydrodynamique généralisée du changement de conformation coopératif de la dynamine induit par le GTP et réconcilions des résultats expérimentaux apparemment contradictoires par des arguments mécaniques. La dynamique aux temps longs de l'assemblage dynamine-membrane est diffusive et dominée par une friction effective entre dynamine et membrane, ce qui est confirmé expérimentalement. Notre second sujet est le complexe ESCRT-III, qui tubule les membranes planes de l'intérieur. Nous expliquons cette déformation par une instabilité de flambage inédite se produisant lorsque des filaments courbés qui s'attirent se lient à la membrane. Cette hypothèse peut être vérifiée expérimentalement. Un régime métastable pour la membrane plane est mis en évidence, et pourrait être utilisé par la cellule pour former des tubes rapidement. Troisièment, nous nous tournons vers les stéréocils, des protrusions cellulaires à base d'actine essentielles pour l'audition. Nous expliquons leur forme par la dynamique de détachement de protéines liant l'actine, et rendons compte de résultats expérimentaux. Si ces protéines sont autorisées à se réattacher, notre modèle prévoit une transition de phase dynamique vers un état de croissance non-bornée, et des simulations numériques suggèrent que la longueur des protrusions diverge en loi de puissance avec un exposant anormal.</abstract> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Psychologie/explor/BernheimV1/Data/Hal/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000161 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000161 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Psychologie
|area= BernheimV1
|flux= Hal
|étape= Checkpoint
|type= RBID
|clé= Hal:tel-00541655
|texte= Shaping tubes in cells
}}
| This area was generated with Dilib version V0.6.33. |  |