Identification of the interleukin-6/oncostation M response element in the rat tissue inhibitor of metalloproteinases-1 (TIMP-1)Promoter
Identifieur interne : 000E59 ( Main/Exploration ); précédent : 000E58; suivant : 000E60Identification of the interleukin-6/oncostation M response element in the rat tissue inhibitor of metalloproteinases-1 (TIMP-1)Promoter
Auteurs : Marcin Bugno [Allemagne] ; Lutz Graeve [Allemagne] ; Petros Gatsios [Allemagne] ; Aleksander Koj [Allemagne] ; Peter C. Heinrich [Allemagne] ; James Travls [États-Unis] ; Tomasz Kordula [Allemagne]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 1995.
Abstract
The rat tissue inhibitor of metalloproteinases 1 (TIMP-1) gene is expressed in rat hepatocytes, and this expression Is up-regulated by interieukln 6 (IL-6). We report here the cloning of the 5′ flanking region of the rat TIMP-1 gene and identification of an IL-6/oncostatln M (OSM) response element at −64 to −36 which functions In hepatic cells. Within this element we have identified two functional binding sites for transcription factors AP-1 (activatory proteln-1) and STAT (signal transducer and activator of transcription). IL-6/OSM stimulation Induces binding of a protein, identified as STAT3, to the IL-6/0SM response element, while binding of the AP-1 protein was constitutive. Binding sites for both AP-1 and STAT3 are necessary for full responsiveness of the TIMP-1 promoter to IL-6/OSM, as shown by deletion and mutation analysis. Furthermore, the entire IL-6/OSM response element conferred responsiveness onto a heterologous promoter, whereas this has not been observed when AP-1 and STAT elements were separately tested.
Url:
DOI: 10.1093/nar/23.24.5041
Affiliations:
- Allemagne, États-Unis
- District de Cologne, Géorgie (États-Unis), Rhénanie-du-Nord-Westphalie
- Aix-la-Chapelle
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Identification of the interleukin-6/oncostation M response element in the rat tissue inhibitor of metalloproteinases-1 (TIMP-1)Promoter</title>
<author><name sortKey="Bugno, Marcin" sort="Bugno, Marcin" uniqKey="Bugno M" first="Marcin" last="Bugno">Marcin Bugno</name>
</author>
<author><name sortKey="Graeve, Lutz" sort="Graeve, Lutz" uniqKey="Graeve L" first="Lutz" last="Graeve">Lutz Graeve</name>
</author>
<author><name sortKey="Gatsios, Petros" sort="Gatsios, Petros" uniqKey="Gatsios P" first="Petros" last="Gatsios">Petros Gatsios</name>
</author>
<author><name sortKey="Koj, Aleksander" sort="Koj, Aleksander" uniqKey="Koj A" first="Aleksander" last="Koj">Aleksander Koj</name>
</author>
<author><name sortKey="Heinrich, Peter C" sort="Heinrich, Peter C" uniqKey="Heinrich P" first="Peter C." last="Heinrich">Peter C. Heinrich</name>
</author>
<author><name sortKey="Travls, James" sort="Travls, James" uniqKey="Travls J" first="James" last="Travls">James Travls</name>
</author>
<author><name sortKey="Kordula, Tomasz" sort="Kordula, Tomasz" uniqKey="Kordula T" first="Tomasz" last="Kordula">Tomasz Kordula</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:1D7675AFC59A961D0A8354F65AFCCF473B5F7A9C</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1093/nar/23.24.5041</idno>
<idno type="url">https://api.istex.fr/document/1D7675AFC59A961D0A8354F65AFCCF473B5F7A9C/fulltext/pdf</idno>
<idno type="wicri:Area/Main/Corpus">000211</idno>
<idno type="wicri:Area/Main/Curation">000210</idno>
<idno type="wicri:Area/Main/Exploration">000E59</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">000E59</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Identification of the interleukin-6/oncostation M response element in the rat tissue inhibitor of metalloproteinases-1 (TIMP-1)Promoter</title>
<author><name sortKey="Bugno, Marcin" sort="Bugno, Marcin" uniqKey="Bugno M" first="Marcin" last="Bugno">Marcin Bugno</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Molecular Biology, Jagiellonian University, Krakow, Poland, Athens, GA 30602, USA, Aachen</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Cologne</region>
<settlement type="city">Aix-la-Chapelle</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Graeve, Lutz" sort="Graeve, Lutz" uniqKey="Graeve L" first="Lutz" last="Graeve">Lutz Graeve</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry, Rehnisch-Westfalische Technische Hochschule Aachen</wicri:regionArea>
<wicri:noRegion>Rehnisch-Westfalische Technische Hochschule Aachen</wicri:noRegion>
<wicri:noRegion>Rehnisch-Westfalische Technische Hochschule Aachen</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gatsios, Petros" sort="Gatsios, Petros" uniqKey="Gatsios P" first="Petros" last="Gatsios">Petros Gatsios</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry, Rehnisch-Westfalische Technische Hochschule Aachen</wicri:regionArea>
<wicri:noRegion>Rehnisch-Westfalische Technische Hochschule Aachen</wicri:noRegion>
<wicri:noRegion>Rehnisch-Westfalische Technische Hochschule Aachen</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Koj, Aleksander" sort="Koj, Aleksander" uniqKey="Koj A" first="Aleksander" last="Koj">Aleksander Koj</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Molecular Biology, Jagiellonian University, Krakow, Poland, Athens, GA 30602, USA, Aachen</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Cologne</region>
<settlement type="city">Aix-la-Chapelle</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Heinrich, Peter C" sort="Heinrich, Peter C" uniqKey="Heinrich P" first="Peter C." last="Heinrich">Peter C. Heinrich</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry, Rehnisch-Westfalische Technische Hochschule Aachen</wicri:regionArea>
<wicri:noRegion>Rehnisch-Westfalische Technische Hochschule Aachen</wicri:noRegion>
<wicri:noRegion>Rehnisch-Westfalische Technische Hochschule Aachen</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Travls, James" sort="Travls, James" uniqKey="Travls J" first="James" last="Travls">James Travls</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, The University of Georgia Athens, GA 30602</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
<affiliation></affiliation>
<affiliation></affiliation>
</author>
<author><name sortKey="Kordula, Tomasz" sort="Kordula, Tomasz" uniqKey="Kordula T" first="Tomasz" last="Kordula">Tomasz Kordula</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Molecular Biology, Jagiellonian University, Krakow, Poland, Athens, GA 30602, USA, Aachen</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Cologne</region>
<settlement type="city">Aix-la-Chapelle</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Nucleic Acids Research</title>
<idno type="ISSN">0305-1048</idno>
<idno type="eISSN">1362-4962</idno>
<imprint><publisher>Oxford University Press</publisher>
<date type="published" when="1995">1995</date>
<biblScope unit="volume">23</biblScope>
<biblScope unit="issue">24</biblScope>
<biblScope unit="page" from="5041">5041</biblScope>
<biblScope unit="page" to="5047">5047</biblScope>
</imprint>
<idno type="ISSN">0305-1048</idno>
</series>
<idno type="istex">1D7675AFC59A961D0A8354F65AFCCF473B5F7A9C</idno>
<idno type="DOI">10.1093/nar/23.24.5041</idno>
<idno type="ArticleID">23.24.5041</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0305-1048</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract">The rat tissue inhibitor of metalloproteinases 1 (TIMP-1) gene is expressed in rat hepatocytes, and this expression Is up-regulated by interieukln 6 (IL-6). We report here the cloning of the 5′ flanking region of the rat TIMP-1 gene and identification of an IL-6/oncostatln M (OSM) response element at −64 to −36 which functions In hepatic cells. Within this element we have identified two functional binding sites for transcription factors AP-1 (activatory proteln-1) and STAT (signal transducer and activator of transcription). IL-6/OSM stimulation Induces binding of a protein, identified as STAT3, to the IL-6/0SM response element, while binding of the AP-1 protein was constitutive. Binding sites for both AP-1 and STAT3 are necessary for full responsiveness of the TIMP-1 promoter to IL-6/OSM, as shown by deletion and mutation analysis. Furthermore, the entire IL-6/OSM response element conferred responsiveness onto a heterologous promoter, whereas this has not been observed when AP-1 and STAT elements were separately tested.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>États-Unis</li>
</country>
<region><li>District de Cologne</li>
<li>Géorgie (États-Unis)</li>
<li>Rhénanie-du-Nord-Westphalie</li>
</region>
<settlement><li>Aix-la-Chapelle</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Bugno, Marcin" sort="Bugno, Marcin" uniqKey="Bugno M" first="Marcin" last="Bugno">Marcin Bugno</name>
</region>
<name sortKey="Gatsios, Petros" sort="Gatsios, Petros" uniqKey="Gatsios P" first="Petros" last="Gatsios">Petros Gatsios</name>
<name sortKey="Graeve, Lutz" sort="Graeve, Lutz" uniqKey="Graeve L" first="Lutz" last="Graeve">Lutz Graeve</name>
<name sortKey="Heinrich, Peter C" sort="Heinrich, Peter C" uniqKey="Heinrich P" first="Peter C." last="Heinrich">Peter C. Heinrich</name>
<name sortKey="Koj, Aleksander" sort="Koj, Aleksander" uniqKey="Koj A" first="Aleksander" last="Koj">Aleksander Koj</name>
<name sortKey="Kordula, Tomasz" sort="Kordula, Tomasz" uniqKey="Kordula T" first="Tomasz" last="Kordula">Tomasz Kordula</name>
</country>
<country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Travls, James" sort="Travls, James" uniqKey="Travls J" first="James" last="Travls">James Travls</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/SchutzV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E59 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E59 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Musique |area= SchutzV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:1D7675AFC59A961D0A8354F65AFCCF473B5F7A9C |texte= Identification of the interleukin-6/oncostation M response element in the rat tissue inhibitor of metalloproteinases-1 (TIMP-1)Promoter }}
This area was generated with Dilib version V0.6.38. |