Long‐term, noninvasive imaging of regulated gene expression in living mice
Identifieur interne : 001054 ( Main/Corpus ); précédent : 001053; suivant : 001055Long‐term, noninvasive imaging of regulated gene expression in living mice
Auteurs : Mazahir T. Hasan ; Kai Schönig ; Stefan Berger ; Walter Graewe ; Hermann BujardSource :
- genesis [ 1526-954X ] ; 2001-03.
English descriptors
- KwdEn :
Abstract
Summary: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long‐term studies of gene function in individual mice. genesis 29:116–122, 2001. © 2001 Wiley‐Liss, Inc.
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DOI: 10.1002/gene.1014
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ISTEX:F023DFDE4CFF32F55FA4033B936EC624163ED56DLe document en format XML
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We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long‐term studies of gene function in individual mice. genesis 29:116–122, 2001. © 2001 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Mazahir T. Hasan</name><affiliations><json:string>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</json:string></affiliations></json:item><json:item><name>Kai Schönig</name><affiliations><json:string>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</json:string></affiliations></json:item><json:item><name>Stefan Berger</name><affiliations><json:string>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</json:string></affiliations></json:item><json:item><name>Walter Graewe</name><affiliations><json:string>Hamamatsu Photonics Deutschland GmbH, Herrsching, Germany</json:string></affiliations></json:item><json:item><name>Hermann Bujard</name><affiliations><json:string>Zentrum für Molekulare Biologie der Universitaet Heidelberg (ZMBH), Heidelberg, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>tetracycline</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>regulatable transcription</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cre recombinase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>firefly luciferase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>bioluminescence</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>transgenics</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Summary: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. 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