Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen
Identifieur interne : 000A79 ( Main/Corpus ); précédent : 000A78; suivant : 000A80Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen
Auteurs : Klaus Hempel ; K. M NnlSource :
- Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie [ 0365-5423 ] ; 1967-12-01.
Abstract
Summary: After injection of H-3-tyrosine the synthesis of H-3-dopa was measured in various organs (adrenal glands, brain stem, heart, liver, kidney, spleen, pancreas and blood) of fowl and cat. The isolation of H-3-dopa was carried out in the following way: 1. The acid soluble fraction was fractionated by high voltage paper electrophoresis in a pyridine-acetic acid-buffer. 2. The tyrosine-dopa-spot was eluated and descending paper chromatography was carried out. This system separated tyrosine from dopa. 3. The dopa-spot was eluated again, and dopa was characterized by its complex with boric acid in paper high voltage electrophoresis in a boric acid-sodium hydroxide-buffer. After inhibition of the dopa decarboxylase by NSD-1034 [N-methyl-N-(3-hydroxybenzyl)hydrazine dihydrogen phosphate] H-3-dopa represented in adrenal glands of domestic fowl 4% and in those of cats 5% of the total radioactivity. Under these conditions in all the other organs investigated H-3-dopa could not be detected. Without NSD-1034 H-3-dopa was undetectable in the adrenals, too. In experiments with cats the H-3-dopa concentration in the venous blood of the adrenals was measured. In some samples H-3-dopa represented up to 3% of the total radioactivity. Nearly the total amount of H-3-dopa formed in the adrenals was secreted into the venous blood, and only 5% of the total amount were detected in the gland at the end of the experiments. This demonstrates that dopa cannot be stored by the adrenals. We failed to detect H-3-dopa in blood from any other organ. The concentration of dopa in the adrenals was about 1 μg/g wet weight after inhibition of dopa decarboxylase and without inhibition it was <0.2 μg/g wet weight. The dopa synthesis rate in adrenals was calculated from the H-3-dopa activity in the gland and in the venous blood and from the specific activity of its precursor H-3-tyrosine. One adrenal of the cat synthetized 0.02 μg dopa/min·kg body weight (=1.2 mμMol/min·1 g adrenal).
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DOI: 10.1007/BF01047656
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="de">Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</title><author><name sortKey="Hempel, Klaus" sort="Hempel, Klaus" uniqKey="Hempel K" first="Klaus" last="Hempel">Klaus Hempel</name><affiliation><mods:affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="M Nnl, K" sort="M Nnl, K" uniqKey="M Nnl K" first="K." last="M Nnl">K. M Nnl</name><affiliation><mods:affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:EC677C8E08510998577B665E25D42168D8502016</idno><date when="1967" year="1967">1967</date><idno type="doi">10.1007/BF01047656</idno><idno type="url">https://api.istex.fr/document/EC677C8E08510998577B665E25D42168D8502016/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000A79</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="de">Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</title><author><name sortKey="Hempel, Klaus" sort="Hempel, Klaus" uniqKey="Hempel K" first="Klaus" last="Hempel">Klaus Hempel</name><affiliation><mods:affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="M Nnl, K" sort="M Nnl, K" uniqKey="M Nnl K" first="K." last="M Nnl">K. M Nnl</name><affiliation><mods:affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie</title><title level="j" type="abbrev">Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path.</title><idno type="ISSN">0365-5423</idno><idno type="eISSN">1432-1912</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1967-12-01">1967-12-01</date><biblScope unit="volume">257</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="391">391</biblScope><biblScope unit="page" to="408">408</biblScope></imprint><idno type="ISSN">0365-5423</idno></series><idno type="istex">EC677C8E08510998577B665E25D42168D8502016</idno><idno type="DOI">10.1007/BF01047656</idno><idno type="ArticleID">Art1</idno><idno type="ArticleID">BF01047656</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0365-5423</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="de">de</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: After injection of H-3-tyrosine the synthesis of H-3-dopa was measured in various organs (adrenal glands, brain stem, heart, liver, kidney, spleen, pancreas and blood) of fowl and cat. The isolation of H-3-dopa was carried out in the following way: 1. The acid soluble fraction was fractionated by high voltage paper electrophoresis in a pyridine-acetic acid-buffer. 2. The tyrosine-dopa-spot was eluated and descending paper chromatography was carried out. This system separated tyrosine from dopa. 3. The dopa-spot was eluated again, and dopa was characterized by its complex with boric acid in paper high voltage electrophoresis in a boric acid-sodium hydroxide-buffer. After inhibition of the dopa decarboxylase by NSD-1034 [N-methyl-N-(3-hydroxybenzyl)hydrazine dihydrogen phosphate] H-3-dopa represented in adrenal glands of domestic fowl 4% and in those of cats 5% of the total radioactivity. Under these conditions in all the other organs investigated H-3-dopa could not be detected. Without NSD-1034 H-3-dopa was undetectable in the adrenals, too. In experiments with cats the H-3-dopa concentration in the venous blood of the adrenals was measured. In some samples H-3-dopa represented up to 3% of the total radioactivity. Nearly the total amount of H-3-dopa formed in the adrenals was secreted into the venous blood, and only 5% of the total amount were detected in the gland at the end of the experiments. This demonstrates that dopa cannot be stored by the adrenals. We failed to detect H-3-dopa in blood from any other organ. The concentration of dopa in the adrenals was about 1 μg/g wet weight after inhibition of dopa decarboxylase and without inhibition it was <0.2 μg/g wet weight. The dopa synthesis rate in adrenals was calculated from the H-3-dopa activity in the gland and in the venous blood and from the specific activity of its precursor H-3-tyrosine. One adrenal of the cat synthetized 0.02 μg dopa/min·kg body weight (=1.2 mμMol/min·1 g adrenal).</div></front></TEI><istex><corpusName>springer</corpusName><author><json:item><name>Klaus Hempel</name><affiliations><json:string>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</json:string><json:string>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</json:string></affiliations></json:item><json:item><name>Heinrich F. K. Männl</name><affiliations><json:string>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</json:string><json:string>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>deu</json:string></lang><value>Dopa formation</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Tyrosine hydroxylase</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Dopa decarboxylase</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Adrenal gland</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Cat</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Dopa-Bildung</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Tyrosin-Hydroxylase</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Dopa-Decarboxylase</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Nebenniere</value></json:item><json:item><lang><json:string>deu</json:string></lang><value>Katze</value></json:item></subject><language><json:string>deu</json:string></language><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>443 x 661 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>10</keywordCount><abstractCharCount>1983</abstractCharCount><pdfWordCount>6632</pdfWordCount><pdfCharCount>40913</pdfCharCount><pdfPageCount>18</pdfPageCount><abstractWordCount>307</abstractWordCount></qualityIndicators><title>Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</title><genre><json:string>research-article</json:string></genre><host><volume>257</volume><pages><last>408</last><first>391</first></pages><issn><json:string>0365-5423</json:string></issn><issue>4</issue><subject><json:item><value>Neurosciences</value></json:item><json:item><value>Pharmacology/Toxicology</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1432-1912</json:string></eissn><title>Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie</title><publicationDate>1967</publicationDate><copyrightDate>1967</copyrightDate></host><publicationDate>1967</publicationDate><copyrightDate>1967</copyrightDate><doi><json:string>10.1007/BF01047656</json:string></doi><id>EC677C8E08510998577B665E25D42168D8502016</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/EC677C8E08510998577B665E25D42168D8502016/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/EC677C8E08510998577B665E25D42168D8502016/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/EC677C8E08510998577B665E25D42168D8502016/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="de">Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</title><title level="a" type="alt" xml:lang="en">The formation of H-3-dopa from H-3-tyrosine and the determination of the dopa synthesis rate in the adrenal gland of the cat under in vivo conditions</title><respStmt xml:id="ISTEX-API" resp="Références bibliographiques récupérées via GROBID" name="ISTEX-API (INIST-CNRS)"></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><availability><p>SPRINGER</p></availability><date>1967-03-05</date></publicationStmt><notesStmt><note>Über einen Teil der Ergebnisse wurde auf der Siebten Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz vorgetragen ( Hempel u. Männl , 1966a) und in Form einer Kurzmitteilung berichtet ( Hempel u. Männl , 1966b).</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="de">Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</title><title level="a" type="alt" xml:lang="en">The formation of H-3-dopa from H-3-tyrosine and the determination of the dopa synthesis rate in the adrenal gland of the cat under in vivo conditions</title><author><persName><forename type="first">Klaus</forename><surname>Hempel</surname></persName><affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</affiliation><affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</affiliation></author><author><persName><forename type="first">Heinrich</forename><surname>Männl</surname></persName><affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</affiliation><affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</affiliation></author></analytic><monogr><title level="j">Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie</title><title level="j" type="abbrev">Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path.</title><idno type="JournalID">210</idno><idno type="pISSN">0365-5423</idno><idno type="eISSN">1432-1912</idno><idno type="IssueArticleCount">6</idno><idno type="VolumeIssueCount">4</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1967-12-01"></date><biblScope unit="volume">257</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="391">391</biblScope><biblScope unit="page" to="408">408</biblScope></imprint></monogr><idno type="istex">EC677C8E08510998577B665E25D42168D8502016</idno><idno type="DOI">10.1007/BF01047656</idno><idno type="ArticleID">Art1</idno><idno type="ArticleID">BF01047656</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1967-03-05</date></creation><langUsage><language ident="de">de</language></langUsage><abstract xml:lang="en"><p>Summary: After injection of H-3-tyrosine the synthesis of H-3-dopa was measured in various organs (adrenal glands, brain stem, heart, liver, kidney, spleen, pancreas and blood) of fowl and cat. The isolation of H-3-dopa was carried out in the following way: 1. The acid soluble fraction was fractionated by high voltage paper electrophoresis in a pyridine-acetic acid-buffer. 2. The tyrosine-dopa-spot was eluated and descending paper chromatography was carried out. This system separated tyrosine from dopa. 3. The dopa-spot was eluated again, and dopa was characterized by its complex with boric acid in paper high voltage electrophoresis in a boric acid-sodium hydroxide-buffer. After inhibition of the dopa decarboxylase by NSD-1034 [N-methyl-N-(3-hydroxybenzyl)hydrazine dihydrogen phosphate] H-3-dopa represented in adrenal glands of domestic fowl 4% and in those of cats 5% of the total radioactivity. Under these conditions in all the other organs investigated H-3-dopa could not be detected. Without NSD-1034 H-3-dopa was undetectable in the adrenals, too. In experiments with cats the H-3-dopa concentration in the venous blood of the adrenals was measured. In some samples H-3-dopa represented up to 3% of the total radioactivity. Nearly the total amount of H-3-dopa formed in the adrenals was secreted into the venous blood, and only 5% of the total amount were detected in the gland at the end of the experiments. This demonstrates that dopa cannot be stored by the adrenals. We failed to detect H-3-dopa in blood from any other organ. The concentration of dopa in the adrenals was about 1 μg/g wet weight after inhibition of dopa decarboxylase and without inhibition it was <0.2 μg/g wet weight. The dopa synthesis rate in adrenals was calculated from the H-3-dopa activity in the gland and in the venous blood and from the specific activity of its precursor H-3-tyrosine. One adrenal of the cat synthetized 0.02 μg dopa/min·kg body weight (=1.2 mμMol/min·1 g adrenal).</p></abstract><abstract xml:lang="de"><p>Zusammenfassung: Nach Injektion von H-3-Tyrosin wurde die Bildung von H-3-Dopa in verschiedenen Organen (Nebenniere, Hirnstamm, Herz, Leber, Niere, Milz, Pankreas und Blut) des Huhnes und der Katze gemessen. Die Isolierung von H-3-Dopa wurde wie folgt durchgeführt: 1. Die säurelösliche Fraktion wurde durch Papierhochspannungselektrophorese im Pyridin-Eisessig-Puffer vorgetrennt. 2. Die Tyrosin-Dopa-Bande wurde eluiert und papierchromatographisch aufgetrennt. 3. Die Dopa-Bande wurde erneut eluiert und Dopa wurde als Borat-Komplex durch Papierhochspannungselektrophorese in einem Borat-Puffer charakterisiert. Nach Hemmung der Dopa-Decarboxylase mit NSD-1034 [N-Methyl-N-(3-hydroxybenzyl)hydrazin-dihydrogenphosphat] betrug der Anteil des H-3-Dopa an der Gesamtaktivität der säurelöslichen Fraktion der Nebenniere beim Huhn 4% und bei der Katze 5%. Unter diesen Bedingungen konnte H-3-Dopa in allen anderen untersuchten Organen nicht nachgewiesen werden. Ohne NSD-1034 fand sich auch in der Nebenniere kein H-3-Dopa. Bei Versuchen an Katzen wurde die H-3-Dopa-Konzentration im venösen Blut der Nebenniere gemessen. In einigen Blutproben betrug die H-3-Dopa-Aktivität bis zu 3% der Gesamtaktivität. Nahezu die gesamte von der Nebenniere gebildete Menge an H-3-Dopa wurde an das venöse Blut abgegeben. Nur 5% des neugebildeten Dopa waren am Ende des Versuches in der Nebenniere nachweisbar. Das zeigt, daß Dopa von der Nebenniere nicht gespeichert wird. Im Blut anderer Organe fand sich hingegen kein Dopa. Die Dopa-Konzentration der Nebenniere betrug nach Blockierung der Dopa-Decarboxylase ca. 1 μg/g Naßgewebe und war ohne Hemmung <0,2 μg/g. Die Dopa-Neubildungsrate der Nebenniere wurde abgeschätzt aus dem H-3-Dopa-Gehalt der Nebenniere sowie aus dem zeitlichen Verlauf der H-3-Dopa-Aktivität und der spezifischen Aktivität des freien H-3-Tyrosins. Bei der Katze werden von einer einzelnen Nebenniere etwa $$\frac{{0,02{{\mu}}g}}{{\min /kg}}$$ Dopa gebildet $$\left( { = \frac{{1,2{{m\mu Mol}}}}{{\min /1{{gNebenniere}}}}} \right)$$ .</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Neurosciences</term></item><item><term>Pharmacology/Toxicology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1967-03-05">Created</change><change when="1967-12-01">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-3-2">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/EC677C8E08510998577B665E25D42168D8502016/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, Publisher found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>210</JournalID><JournalPrintISSN>0365-5423</JournalPrintISSN><JournalElectronicISSN>1432-1912</JournalElectronicISSN><JournalTitle>Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie</JournalTitle><JournalAbbreviatedTitle>Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path.</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Neurosciences</JournalSubject><JournalSubject Type="Secondary">Pharmacology/Toxicology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>257</VolumeIDStart><VolumeIDEnd>257</VolumeIDEnd><VolumeIssueCount>4</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>4</IssueIDStart><IssueIDEnd>4</IssueIDEnd><IssueArticleCount>6</IssueArticleCount><IssueHistory><CoverDate><DateString>1967</DateString><Year>1967</Year><Month>12</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1967</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art1"><ArticleInfo Language="De" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF01047656</ArticleID><ArticleDOI>10.1007/BF01047656</ArticleDOI><ArticleSequenceNumber>1</ArticleSequenceNumber><ArticleTitle Language="De">Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</ArticleTitle><ArticleTitle Language="En">The formation of H-3-dopa from H-3-tyrosine and the determination of the dopa synthesis rate in the adrenal gland of the cat under in vivo conditions</ArticleTitle><ArticleFirstPage>391</ArticleFirstPage><ArticleLastPage>408</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>10</Month><Day>11</Day></RegistrationDate><Received><Year>1967</Year><Month>3</Month><Day>5</Day></Received></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1967</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>210</JournalID><VolumeIDStart>257</VolumeIDStart><VolumeIDEnd>257</VolumeIDEnd><IssueIDStart>4</IssueIDStart><IssueIDEnd>4</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Klaus</GivenName><FamilyName>Hempel</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Heinrich</GivenName><GivenName>F.</GivenName><GivenName>K.</GivenName><FamilyName>Männl</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgName>Institut für Medizinische Isotopenforschung der Universität Köln</OrgName><OrgAddress><Country>Germany</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>Institut für Medizinische Strahlenkunde der Universität Würzburg</OrgName><OrgAddress><Country>Germany</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>After injection of H-3-tyrosine the synthesis of H-3-dopa was measured in various organs (adrenal glands, brain stem, heart, liver, kidney, spleen, pancreas and blood) of fowl and cat. The isolation of H-3-dopa was carried out in the following way:<OrderedList><ListItem><ItemNumber>1.</ItemNumber><ItemContent><Para>The acid soluble fraction was fractionated by high voltage paper electrophoresis in a pyridine-acetic acid-buffer.</Para></ItemContent></ListItem><ListItem><ItemNumber>2.</ItemNumber><ItemContent><Para>The tyrosine-dopa-spot was eluated and descending paper chromatography was carried out. This system separated tyrosine from dopa.</Para></ItemContent></ListItem><ListItem><ItemNumber>3.</ItemNumber><ItemContent><Para>The dopa-spot was eluated again, and dopa was characterized by its complex with boric acid in paper high voltage electrophoresis in a boric acid-sodium hydroxide-buffer.</Para></ItemContent></ListItem></OrderedList></Para><Para>After inhibition of the dopa decarboxylase by NSD-1034 [N-methyl-N-(3-hydroxybenzyl)hydrazine dihydrogen phosphate] H-3-dopa represented in adrenal glands of domestic fowl 4% and in those of cats 5% of the total radioactivity. Under these conditions in all the other organs investigated H-3-dopa could not be detected. Without NSD-1034 H-3-dopa was undetectable in the adrenals, too.</Para><Para>In experiments with cats the H-3-dopa concentration in the venous blood of the adrenals was measured. In some samples H-3-dopa represented up to 3% of the total radioactivity. Nearly the total amount of H-3-dopa formed in the adrenals was secreted into the venous blood, and only 5% of the total amount were detected in the gland at the end of the experiments. This demonstrates that dopa cannot be stored by the adrenals. We failed to detect H-3-dopa in blood from any other organ.</Para><Para>The concentration of dopa in the adrenals was about 1 μg/g wet weight after inhibition of dopa decarboxylase and without inhibition it was <0.2 μg/g wet weight.</Para><Para>The dopa synthesis rate in adrenals was calculated from the H-3-dopa activity in the gland and in the venous blood and from the specific activity of its precursor H-3-tyrosine. One adrenal of the cat synthetized 0.02 μg dopa/min·kg body weight (=1.2 mμMol/min·1 g adrenal).</Para></Abstract><Abstract ID="Abs2" Language="De"><Heading>Zusammenfassung</Heading><Para>Nach Injektion von H-3-Tyrosin wurde die Bildung von H-3-Dopa in verschiedenen Organen (Nebenniere, Hirnstamm, Herz, Leber, Niere, Milz, Pankreas und Blut) des Huhnes und der Katze gemessen. Die Isolierung von H-3-Dopa wurde wie folgt durchgeführt:<OrderedList><ListItem><ItemNumber>1.</ItemNumber><ItemContent><Para>Die säurelösliche Fraktion wurde durch Papierhochspannungselektrophorese im Pyridin-Eisessig-Puffer vorgetrennt.</Para></ItemContent></ListItem><ListItem><ItemNumber>2.</ItemNumber><ItemContent><Para>Die Tyrosin-Dopa-Bande wurde eluiert und papierchromatographisch aufgetrennt.</Para></ItemContent></ListItem><ListItem><ItemNumber>3.</ItemNumber><ItemContent><Para>Die Dopa-Bande wurde erneut eluiert und Dopa wurde als Borat-Komplex durch Papierhochspannungselektrophorese in einem Borat-Puffer charakterisiert.</Para></ItemContent></ListItem></OrderedList></Para><Para>Nach Hemmung der Dopa-Decarboxylase mit NSD-1034 [N-Methyl-N-(3-hydroxybenzyl)hydrazin-dihydrogenphosphat] betrug der Anteil des H-3-Dopa an der Gesamtaktivität der säurelöslichen Fraktion der Nebenniere beim Huhn 4% und bei der Katze 5%. Unter diesen Bedingungen konnte H-3-Dopa in allen anderen untersuchten Organen nicht nachgewiesen werden. Ohne NSD-1034 fand sich auch in der Nebenniere kein H-3-Dopa.</Para><Para>Bei Versuchen an Katzen wurde die H-3-Dopa-Konzentration im venösen Blut der Nebenniere gemessen. In einigen Blutproben betrug die H-3-Dopa-Aktivität bis zu 3% der Gesamtaktivität. Nahezu die gesamte von der Nebenniere gebildete Menge an H-3-Dopa wurde an das venöse Blut abgegeben. Nur 5% des neugebildeten Dopa waren am Ende des Versuches in der Nebenniere nachweisbar. Das zeigt, daß Dopa von der Nebenniere nicht gespeichert wird. Im Blut anderer Organe fand sich hingegen kein Dopa.</Para><Para>Die Dopa-Konzentration der Nebenniere betrug nach Blockierung der Dopa-Decarboxylase ca. 1 μg/g Naßgewebe und war ohne Hemmung <0,2 μg/g.</Para><Para>Die Dopa-Neubildungsrate der Nebenniere wurde abgeschätzt aus dem H-3-Dopa-Gehalt der Nebenniere sowie aus dem zeitlichen Verlauf der H-3-Dopa-Aktivität und der spezifischen Aktivität des freien H-3-Tyrosins. Bei der Katze werden von einer einzelnen Nebenniere etwa<InlineEquation ID="IE1"><InlineMediaObject><ImageObject FileRef="210_2004_Article_BF01047656_TeX2GIFIE1.gif" Format="GIF" Color="BlackWhite" Type="Linedraw" Rendition="HTML"></ImageObject></InlineMediaObject><EquationSource Format="TEX"> $$\frac{{0,02{{\mu}}g}}{{\min /kg}}$$ </EquationSource></InlineEquation> Dopa gebildet<InlineEquation ID="IE2"><InlineMediaObject><ImageObject FileRef="210_2004_Article_BF01047656_TeX2GIFIE2.gif" Format="GIF" Color="BlackWhite" Type="Linedraw" Rendition="HTML"></ImageObject></InlineMediaObject><EquationSource Format="TEX"> $$\left( { = \frac{{1,2{{m\mu Mol}}}}{{\min /1{{gNebenniere}}}}} \right)$$ </EquationSource></InlineEquation>.</Para></Abstract><KeywordGroup Language="En"><Heading>Key-Words</Heading><Keyword>Dopa formation</Keyword><Keyword>Tyrosine hydroxylase</Keyword><Keyword>Dopa decarboxylase</Keyword><Keyword>Adrenal gland</Keyword><Keyword>Cat</Keyword></KeywordGroup><KeywordGroup Language="De"><Heading>Schlüsselwörter</Heading><Keyword>Dopa-Bildung</Keyword><Keyword>Tyrosin-Hydroxylase</Keyword><Keyword>Dopa-Decarboxylase</Keyword><Keyword>Nebenniere</Keyword><Keyword>Katze</Keyword></KeywordGroup><ArticleNote Type="Misc"><SimplePara>Über einen Teil der Ergebnisse wurde auf der Siebten Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz vorgetragen (<Emphasis Type="SmallCaps">Hempel</Emphasis> u.<Emphasis Type="SmallCaps">Männl</Emphasis>, 1966a) und in Form einer Kurzmitteilung berichtet (<Emphasis Type="SmallCaps">Hempel</Emphasis> u.<Emphasis Type="SmallCaps">Männl</Emphasis>, 1966b).</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="de"><title>Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="de"><title>Über die Bildung von H-3-Dopa aus H-3-Tyrosin und die Bestimmung der Dopa-Neubildungsrate in der Nebenniere des Huhnes und der Katze unter in vivo-Bedingungen</title></titleInfo><titleInfo lang="en"><title>The formation of H-3-dopa from H-3-tyrosine and the determination of the dopa synthesis rate in the adrenal gland of the cat under in vivo conditions</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>The formation of H-3-dopa from H-3-tyrosine and the determination of the dopa synthesis rate in the adrenal gland of the cat under in vivo conditions</title></titleInfo><name type="personal"><namePart type="given">Klaus</namePart><namePart type="family">Hempel</namePart><affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</affiliation><affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Heinrich</namePart><namePart type="given">F.</namePart><namePart type="given">K.</namePart><namePart type="family">Männl</namePart><affiliation>Institut für Medizinische Isotopenforschung der Universität Köln, Germany</affiliation><affiliation>Institut für Medizinische Strahlenkunde der Universität Würzburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">1967-03-05</dateCreated><dateIssued encoding="w3cdtf">1967-12-01</dateIssued><copyrightDate encoding="w3cdtf">1967</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">de</languageTerm><languageTerm type="code" authority="iso639-2b">deu</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Summary: After injection of H-3-tyrosine the synthesis of H-3-dopa was measured in various organs (adrenal glands, brain stem, heart, liver, kidney, spleen, pancreas and blood) of fowl and cat. The isolation of H-3-dopa was carried out in the following way: 1. The acid soluble fraction was fractionated by high voltage paper electrophoresis in a pyridine-acetic acid-buffer. 2. The tyrosine-dopa-spot was eluated and descending paper chromatography was carried out. This system separated tyrosine from dopa. 3. The dopa-spot was eluated again, and dopa was characterized by its complex with boric acid in paper high voltage electrophoresis in a boric acid-sodium hydroxide-buffer. After inhibition of the dopa decarboxylase by NSD-1034 [N-methyl-N-(3-hydroxybenzyl)hydrazine dihydrogen phosphate] H-3-dopa represented in adrenal glands of domestic fowl 4% and in those of cats 5% of the total radioactivity. Under these conditions in all the other organs investigated H-3-dopa could not be detected. Without NSD-1034 H-3-dopa was undetectable in the adrenals, too. In experiments with cats the H-3-dopa concentration in the venous blood of the adrenals was measured. In some samples H-3-dopa represented up to 3% of the total radioactivity. Nearly the total amount of H-3-dopa formed in the adrenals was secreted into the venous blood, and only 5% of the total amount were detected in the gland at the end of the experiments. This demonstrates that dopa cannot be stored by the adrenals. We failed to detect H-3-dopa in blood from any other organ. The concentration of dopa in the adrenals was about 1 μg/g wet weight after inhibition of dopa decarboxylase and without inhibition it was <0.2 μg/g wet weight. The dopa synthesis rate in adrenals was calculated from the H-3-dopa activity in the gland and in the venous blood and from the specific activity of its precursor H-3-tyrosine. One adrenal of the cat synthetized 0.02 μg dopa/min·kg body weight (=1.2 mμMol/min·1 g adrenal).</abstract><abstract lang="de">Zusammenfassung: Nach Injektion von H-3-Tyrosin wurde die Bildung von H-3-Dopa in verschiedenen Organen (Nebenniere, Hirnstamm, Herz, Leber, Niere, Milz, Pankreas und Blut) des Huhnes und der Katze gemessen. Die Isolierung von H-3-Dopa wurde wie folgt durchgeführt: 1. Die säurelösliche Fraktion wurde durch Papierhochspannungselektrophorese im Pyridin-Eisessig-Puffer vorgetrennt. 2. Die Tyrosin-Dopa-Bande wurde eluiert und papierchromatographisch aufgetrennt. 3. Die Dopa-Bande wurde erneut eluiert und Dopa wurde als Borat-Komplex durch Papierhochspannungselektrophorese in einem Borat-Puffer charakterisiert. Nach Hemmung der Dopa-Decarboxylase mit NSD-1034 [N-Methyl-N-(3-hydroxybenzyl)hydrazin-dihydrogenphosphat] betrug der Anteil des H-3-Dopa an der Gesamtaktivität der säurelöslichen Fraktion der Nebenniere beim Huhn 4% und bei der Katze 5%. Unter diesen Bedingungen konnte H-3-Dopa in allen anderen untersuchten Organen nicht nachgewiesen werden. Ohne NSD-1034 fand sich auch in der Nebenniere kein H-3-Dopa. Bei Versuchen an Katzen wurde die H-3-Dopa-Konzentration im venösen Blut der Nebenniere gemessen. In einigen Blutproben betrug die H-3-Dopa-Aktivität bis zu 3% der Gesamtaktivität. Nahezu die gesamte von der Nebenniere gebildete Menge an H-3-Dopa wurde an das venöse Blut abgegeben. Nur 5% des neugebildeten Dopa waren am Ende des Versuches in der Nebenniere nachweisbar. Das zeigt, daß Dopa von der Nebenniere nicht gespeichert wird. Im Blut anderer Organe fand sich hingegen kein Dopa. Die Dopa-Konzentration der Nebenniere betrug nach Blockierung der Dopa-Decarboxylase ca. 1 μg/g Naßgewebe und war ohne Hemmung <0,2 μg/g. Die Dopa-Neubildungsrate der Nebenniere wurde abgeschätzt aus dem H-3-Dopa-Gehalt der Nebenniere sowie aus dem zeitlichen Verlauf der H-3-Dopa-Aktivität und der spezifischen Aktivität des freien H-3-Tyrosins. Bei der Katze werden von einer einzelnen Nebenniere etwa $$\frac{{0,02{{\mu}}g}}{{\min /kg}}$$ Dopa gebildet $$\left( { = \frac{{1,2{{m\mu Mol}}}}{{\min /1{{gNebenniere}}}}} \right)$$ .</abstract><note>Über einen Teil der Ergebnisse wurde auf der Siebten Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz vorgetragen ( Hempel u. Männl , 1966a) und in Form einer Kurzmitteilung berichtet ( Hempel u. Männl , 1966b).</note><relatedItem type="host"><titleInfo><title>Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie</title></titleInfo><titleInfo type="abbreviated"><title>Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path.</title></titleInfo><genre type="Journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1967-12-01</dateIssued><copyrightDate encoding="w3cdtf">1967</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Neurosciences</topic><topic>Pharmacology/Toxicology</topic></subject><identifier type="ISSN">0365-5423</identifier><identifier type="eISSN">1432-1912</identifier><identifier type="JournalID">210</identifier><identifier type="IssueArticleCount">6</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>1967</date><detail type="volume"><number>257</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="pages"><start>391</start><end>408</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1967</recordOrigin></recordInfo></relatedItem><identifier type="istex">EC677C8E08510998577B665E25D42168D8502016</identifier><identifier type="DOI">10.1007/BF01047656</identifier><identifier type="ArticleID">Art1</identifier><identifier type="ArticleID">BF01047656</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Springer-Verlag, 1967</recordOrigin></recordInfo></mods></metadata><enrichments><istex:refBibTEI uri="https://api.istex.fr/document/EC677C8E08510998577B665E25D42168D8502016/enrichments/refBib"><teiHeader></teiHeader><text><front></front><body></body><back><listBibl><biblStruct xml:id="b0"><analytic><title level="a" type="main">Synthese tritiummarkie1'ter Aminos~uren hoher spezifischer Aktivit~it</title><author><persName><forename type="first">Bi~ko~er</forename></persName></author><author><persName><forename type="first">L</forename><forename type="middle">K</forename><surname>Hemp~l</surname></persName></author></analytic><monogr><title level="j">Chem. 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