Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects
Identifieur interne : 000977 ( Main/Corpus ); précédent : 000976; suivant : 000978Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects
Auteurs : Frank Schwarz ; Daniel Ferrari ; Martin Sager ; Monika Herten ; Brigitte Hartig ; Jürgen BeckerSource :
- Clinical Oral Implants Research [ 0905-7161 ] ; 2009-11.
English descriptors
Abstract
Objectives: The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model. Material and methods: Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (n=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm2) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis. Results: At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups. Conclusion: It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.
Url:
DOI: 10.1111/j.1600-0501.2009.01796.x
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ISTEX:37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects</title><author><name sortKey="Schwarz, Frank" sort="Schwarz, Frank" uniqKey="Schwarz F" first="Frank" last="Schwarz">Frank Schwarz</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Ferrari, Daniel" sort="Ferrari, Daniel" uniqKey="Ferrari D" first="Daniel" last="Ferrari">Daniel Ferrari</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Sager, Martin" sort="Sager, Martin" uniqKey="Sager M" first="Martin" last="Sager">Martin Sager</name><affiliation><mods:affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Herten, Monika" sort="Herten, Monika" uniqKey="Herten M" first="Monika" last="Herten">Monika Herten</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hartig, Brigitte" sort="Hartig, Brigitte" uniqKey="Hartig B" first="Brigitte" last="Hartig">Brigitte Hartig</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Becker, Jurgen" sort="Becker, Jurgen" uniqKey="Becker J" first="Jürgen" last="Becker">Jürgen Becker</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno 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Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Sager, Martin" sort="Sager, Martin" uniqKey="Sager M" first="Martin" last="Sager">Martin Sager</name><affiliation><mods:affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Herten, Monika" sort="Herten, Monika" uniqKey="Herten M" first="Monika" last="Herten">Monika Herten</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hartig, Brigitte" sort="Hartig, Brigitte" uniqKey="Hartig B" first="Brigitte" last="Hartig">Brigitte Hartig</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Becker, Jurgen" sort="Becker, Jurgen" uniqKey="Becker J" first="Jürgen" last="Becker">Jürgen Becker</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Clinical Oral Implants Research</title><idno type="ISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2009-11">2009-11</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1219">1219</biblScope><biblScope unit="page" to="1230">1230</biblScope></imprint><idno type="ISSN">0905-7161</idno></series><idno type="istex">37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7</idno><idno type="DOI">10.1111/j.1600-0501.2009.01796.x</idno><idno type="ArticleID">CLR1796</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0905-7161</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>animal study</term><term>guided bone regeneration</term><term>immunohistochemistry</term><term>rhBMP‐2</term><term>rhGDF‐5</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objectives: The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model. Material and methods: Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (n=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm2) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis. Results: At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups. Conclusion: It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Frank Schwarz</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Daniel Ferrari</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Martin Sager</name><affiliations><json:string>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Monika Herten</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Brigitte Hartig</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Jürgen Becker</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>animal study</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>guided bone regeneration</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>immunohistochemistry</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>rhBMP‐2</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>rhGDF‐5</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Objectives: The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model. Material and methods: Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (n=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm2) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis. Results: At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups. Conclusion: It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.</abstract><qualityIndicators><score>7.844</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 782.362 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1564</abstractCharCount><pdfWordCount>8841</pdfWordCount><pdfCharCount>54886</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>237</abstractWordCount></qualityIndicators><title>Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects</title><genre><json:string>article</json:string></genre><host><volume>20</volume><pages><total>12</total><last>1230</last><first>1219</first></pages><issn><json:string>0905-7161</json:string></issn><issue>11</issue><genre></genre><language><json:string>unknown</json:string></language><eissn><json:string>1600-0501</json:string></eissn><title>Clinical Oral Implants Research</title><doi><json:string>10.1111/(ISSN)1600-0501</json:string></doi></host><publicationDate>2009</publicationDate><copyrightDate>2009</copyrightDate><doi><json:string>10.1111/j.1600-0501.2009.01796.x</json:string></doi><id>37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>WILEY</p></availability><date>2009</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects</title><author><persName><forename type="first">Frank</forename><surname>Schwarz</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Daniel</forename><surname>Ferrari</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Martin</forename><surname>Sager</surname></persName><affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Monika</forename><surname>Herten</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Brigitte</forename><surname>Hartig</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Jürgen</forename><surname>Becker</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author></analytic><monogr><title level="j">Clinical Oral Implants Research</title><idno type="pISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><idno type="DOI">10.1111/(ISSN)1600-0501</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2009-11"></date><biblScope unit="volume">20</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1219">1219</biblScope><biblScope unit="page" to="1230">1230</biblScope></imprint></monogr><idno type="istex">37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7</idno><idno type="DOI">10.1111/j.1600-0501.2009.01796.x</idno><idno type="ArticleID">CLR1796</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Objectives: The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model. Material and methods: Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (n=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm2) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis. Results: At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups. Conclusion: It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>animal study</term></item><item><term>guided bone regeneration</term></item><item><term>immunohistochemistry</term></item><item><term>rhBMP‐2</term></item><item><term>rhGDF‐5</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2009-11">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1600-0501</doi><issn type="print">0905-7161</issn><issn type="electronic">1600-0501</issn><idGroup><id type="product" value="CLR"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="CLINICAL ORAL IMPLANTS RESEARCH">Clinical Oral Implants Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="11011"><doi origin="wiley">10.1111/clr.2009.20.issue-11</doi><numberingGroup><numbering type="journalVolume" number="20">20</numbering><numbering type="journalIssue" number="11">11</numbering></numberingGroup><coverDate startDate="2009-11">November 2009</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="6" status="forIssue"><doi origin="wiley">10.1111/j.1600-0501.2009.01796.x</doi><idGroup><id type="unit" value="CLR1796"></id><id type="supplier" value="1796"></id></idGroup><countGroup><count type="pageTotal" number="12"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright>© 2009 John Wiley & Sons A/S</copyright><eventGroup><event type="firstOnline" date="2009-08-30"></event><event type="publishedOnlineFinalForm" date="2009-10-11"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:FullText result:FullText" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-12"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="1219">1219</numbering><numbering type="pageLast" number="1230">1230</numbering></numberingGroup><correspondenceTo><b>Correspondence to:</b>
<i>Priv. Doz. Dr Frank Schwarz</i>
Department of Oral Surgery
Westdeutsche Kieferklinik
Heinrich Heine University
D‐40225 Düsseldorf
Germany
Tel.: +49 211 810 4471
Fax: +49 211 811 6550
e‐mail: <email normalForm="frank.schwarz@med.uni-duesseldorf.de">frank.schwarz@med.uni‐duesseldorf.de</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CLR.CLR1796.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory><b>Date:</b> Accepted 26 May 2009</unparsedEditorialHistory><countGroup><count type="figureTotal" number="9"></count><count type="tableTotal" number="6"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="51"></count><count type="wordTotal" number="10769"></count><count type="linksCrossRef" number="85"></count></countGroup><titleGroup><title type="main">Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects</title><title type="shortAuthors">Schwarz et al.</title><title type="short">Coating using rhGDF‐5 and rhBMP‐2</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Frank</givenNames><familyName>Schwarz</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Daniel</givenNames><familyName>Ferrari</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>Martin</givenNames><familyName>Sager</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>Monika</givenNames><familyName>Herten</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>Brigitte</givenNames><familyName>Hartig</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>Jürgen</givenNames><familyName>Becker</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="DE"><unparsedAffiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="DE"><unparsedAffiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">animal study</keyword><keyword xml:id="k2">guided bone regeneration</keyword><keyword xml:id="k3">immunohistochemistry</keyword><keyword xml:id="k4">rhBMP‐2</keyword><keyword xml:id="k5">rhGDF‐5</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p><b>Objectives: </b> The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model.</p><p><b>Material and methods: </b> Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (<i>n</i>=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm<sup>2</sup>) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis.</p><p><b>Results: </b> At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups.</p><p><b>Conclusion: </b> It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.</p><!-- To cite this article:
Schwarz F, Ferrari D, Sager M, Herten M, Hartig B, Becker J. Guided bone regeneration using rhGDF-5- and rhBMP-2-coated natural bone mineral in rat calvarial defects.
Clin. Oral Impl. Res. 20, 2009; 1219–1230.
doi: 10.1111/j.1600-0501.2009.01796.x.
</abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects</title></titleInfo><titleInfo type="abbreviated"><title>Coating using rhGDF‐5 and rhBMP‐2</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects</title></titleInfo><name type="personal"><namePart type="given">Frank</namePart><namePart type="family">Schwarz</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Daniel</namePart><namePart type="family">Ferrari</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Martin</namePart><namePart type="family">Sager</namePart><affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Monika</namePart><namePart type="family">Herten</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Brigitte</namePart><namePart type="family">Hartig</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jürgen</namePart><namePart type="family">Becker</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2009-11</dateIssued><edition>Date: Accepted 26 May 2009</edition><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">9</extent><extent unit="tables">6</extent><extent unit="references">51</extent><extent unit="words">10769</extent></physicalDescription><abstract lang="en">Objectives: The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model. Material and methods: Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (n=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm2) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis. Results: At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups. Conclusion: It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.</abstract><subject lang="en"><genre>Keywords</genre><topic>animal study</topic><topic>guided bone regeneration</topic><topic>immunohistochemistry</topic><topic>rhBMP‐2</topic><topic>rhGDF‐5</topic></subject><relatedItem type="host"><titleInfo><title>Clinical Oral Implants Research</title></titleInfo><genre type="Journal">journal</genre><identifier type="ISSN">0905-7161</identifier><identifier type="eISSN">1600-0501</identifier><identifier type="DOI">10.1111/(ISSN)1600-0501</identifier><identifier type="PublisherID">CLR</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>20</number></detail><detail type="issue"><caption>no.</caption><number>11</number></detail><extent unit="pages"><start>1219</start><end>1230</end><total>12</total></extent></part></relatedItem><identifier type="istex">37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7</identifier><identifier type="DOI">10.1111/j.1600-0501.2009.01796.x</identifier><identifier type="ArticleID">CLR1796</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2009 John Wiley & Sons A/S</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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