Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs
Identifieur interne : 000666 ( Main/Corpus ); précédent : 000665; suivant : 000667Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs
Auteurs : Frank Schwarz ; Daniel Rothamel ; Monika Herten ; Daniel Ferrari ; Martin Sager ; Jürgen BeckerSource :
- Clinical Oral Implants Research [ 0905-7161 ] ; 2008-07.
English descriptors
- KwdEn :
Abstract
Objectives: The aim of the present study was to immunohistochemically evaluate lateral ridge augmentation using a particulated (BOG) or block (BOB) natural bone mineral biocoated with rhGDF‐5 and rhBMP‐2 in dogs. Materials and methods: Three standardized box‐shaped defects were surgically created at the buccal aspect of the alveolar ridge in each quadrant of eight beagle dogs. After 2 months of healing, the chronic‐type defects were randomly allocated in a split‐mouth design to either (i) BOG or (ii) BOB biocoated with (a) rhGDF‐5 or (b) rhBMP‐2, respectively. Uncoated grafts served as controls. After 3 and 8 weeks, dissected blocks were prepared for immunohistochemical [osteocalcin (OC)] and histomorphometrical analysis [e.g. area (mm2) of new bone fill (BF), newly formed mineralized (MT) and non‐mineralized tissue (NMT)]. Results: rhBMP‐2 biocoated BOG revealed significantly highest BF and MT values at 3 (upper and lower jaws – UJ/LJ – compared with BOG) and 8 weeks (UJ – compared with rhGDF‐5). Biocoating of BOB using both rhGDF‐5 and rhBMP‐2 resulted in significantly increased MT values at 8 weeks (UJ/LJ – compared with BOB). In all groups, NMT adjacent to BOG and BOB scaffolds revealed pronounced signs of an OC antigen reactivity. Conclusions: Within the limits of the present study, it was concluded that both rhGDF‐5 and rhBMP‐2 have shown efficacy; however, their bone regenerative effect was markedly influenced by the carrier.
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DOI: 10.1111/j.1600-0501.2008.01537.x-i2
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs</title><author><name sortKey="Schwarz, Frank" sort="Schwarz, Frank" uniqKey="Schwarz F" first="Frank" last="Schwarz">Frank Schwarz</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Rothamel, Daniel" sort="Rothamel, Daniel" uniqKey="Rothamel D" first="Daniel" last="Rothamel">Daniel Rothamel</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Herten, Monika" sort="Herten, Monika" uniqKey="Herten M" first="Monika" last="Herten">Monika Herten</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Ferrari, Daniel" sort="Ferrari, Daniel" uniqKey="Ferrari D" first="Daniel" last="Ferrari">Daniel Ferrari</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Sager, Martin" sort="Sager, Martin" uniqKey="Sager M" first="Martin" last="Sager">Martin Sager</name><affiliation><mods:affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Becker, Jurgen" sort="Becker, Jurgen" uniqKey="Becker J" first="Jürgen" last="Becker">Jürgen Becker</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno 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Rothamel</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Herten, Monika" sort="Herten, Monika" uniqKey="Herten M" first="Monika" last="Herten">Monika Herten</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Ferrari, Daniel" sort="Ferrari, Daniel" uniqKey="Ferrari D" first="Daniel" last="Ferrari">Daniel Ferrari</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Sager, Martin" sort="Sager, Martin" uniqKey="Sager M" first="Martin" last="Sager">Martin Sager</name><affiliation><mods:affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Becker, Jurgen" sort="Becker, Jurgen" uniqKey="Becker J" first="Jürgen" last="Becker">Jürgen Becker</name><affiliation><mods:affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Clinical Oral Implants Research</title><idno type="ISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2008-07">2008-07</date><biblScope unit="volume">19</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="642">642</biblScope><biblScope unit="page" to="652">652</biblScope></imprint><idno type="ISSN">0905-7161</idno></series><idno type="istex">51EDBCE210C43D463D6290B76BE9F8D0419928CA</idno><idno type="DOI">10.1111/j.1600-0501.2008.01537.x-i2</idno><idno type="ArticleID">CLR1537</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0905-7161</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>animal experiments</term><term>bone substitutes</term><term>growth factors</term><term>guided bone regeneration</term><term>morphometric analysis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objectives: The aim of the present study was to immunohistochemically evaluate lateral ridge augmentation using a particulated (BOG) or block (BOB) natural bone mineral biocoated with rhGDF‐5 and rhBMP‐2 in dogs. Materials and methods: Three standardized box‐shaped defects were surgically created at the buccal aspect of the alveolar ridge in each quadrant of eight beagle dogs. After 2 months of healing, the chronic‐type defects were randomly allocated in a split‐mouth design to either (i) BOG or (ii) BOB biocoated with (a) rhGDF‐5 or (b) rhBMP‐2, respectively. Uncoated grafts served as controls. After 3 and 8 weeks, dissected blocks were prepared for immunohistochemical [osteocalcin (OC)] and histomorphometrical analysis [e.g. area (mm2) of new bone fill (BF), newly formed mineralized (MT) and non‐mineralized tissue (NMT)]. Results: rhBMP‐2 biocoated BOG revealed significantly highest BF and MT values at 3 (upper and lower jaws – UJ/LJ – compared with BOG) and 8 weeks (UJ – compared with rhGDF‐5). Biocoating of BOB using both rhGDF‐5 and rhBMP‐2 resulted in significantly increased MT values at 8 weeks (UJ/LJ – compared with BOB). In all groups, NMT adjacent to BOG and BOB scaffolds revealed pronounced signs of an OC antigen reactivity. Conclusions: Within the limits of the present study, it was concluded that both rhGDF‐5 and rhBMP‐2 have shown efficacy; however, their bone regenerative effect was markedly influenced by the carrier.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Frank Schwarz</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Daniel Rothamel</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Monika Herten</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Daniel Ferrari</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Martin Sager</name><affiliations><json:string>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item><json:item><name>Jürgen Becker</name><affiliations><json:string>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>animal experiments</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>bone substitutes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>growth factors</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>guided bone regeneration</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>morphometric analysis</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Objectives: The aim of the present study was to immunohistochemically evaluate lateral ridge augmentation using a particulated (BOG) or block (BOB) natural bone mineral biocoated with rhGDF‐5 and rhBMP‐2 in dogs. Materials and methods: Three standardized box‐shaped defects were surgically created at the buccal aspect of the alveolar ridge in each quadrant of eight beagle dogs. After 2 months of healing, the chronic‐type defects were randomly allocated in a split‐mouth design to either (i) BOG or (ii) BOB biocoated with (a) rhGDF‐5 or (b) rhBMP‐2, respectively. Uncoated grafts served as controls. After 3 and 8 weeks, dissected blocks were prepared for immunohistochemical [osteocalcin (OC)] and histomorphometrical analysis [e.g. area (mm2) of new bone fill (BF), newly formed mineralized (MT) and non‐mineralized tissue (NMT)]. Results: rhBMP‐2 biocoated BOG revealed significantly highest BF and MT values at 3 (upper and lower jaws – UJ/LJ – compared with BOG) and 8 weeks (UJ – compared with rhGDF‐5). Biocoating of BOB using both rhGDF‐5 and rhBMP‐2 resulted in significantly increased MT values at 8 weeks (UJ/LJ – compared with BOB). In all groups, NMT adjacent to BOG and BOB scaffolds revealed pronounced signs of an OC antigen reactivity. Conclusions: Within the limits of the present study, it was concluded that both rhGDF‐5 and rhBMP‐2 have shown efficacy; however, their bone regenerative effect was markedly influenced by the carrier.</abstract><qualityIndicators><score>7.688</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 782.362 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1456</abstractCharCount><pdfWordCount>6279</pdfWordCount><pdfCharCount>39920</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>224</abstractWordCount></qualityIndicators><title>Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs</title><genre><json:string>article</json:string></genre><host><volume>19</volume><pages><total>11</total><last>652</last><first>642</first></pages><issn><json:string>0905-7161</json:string></issn><issue>7</issue><genre></genre><language><json:string>unknown</json:string></language><eissn><json:string>1600-0501</json:string></eissn><title>Clinical Oral Implants Research</title><doi><json:string>10.1111/(ISSN)1600-0501</json:string></doi></host><publicationDate>2008</publicationDate><copyrightDate>2008</copyrightDate><doi><json:string>10.1111/j.1600-0501.2008.01537.x-i2</json:string></doi><id>51EDBCE210C43D463D6290B76BE9F8D0419928CA</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/51EDBCE210C43D463D6290B76BE9F8D0419928CA/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/51EDBCE210C43D463D6290B76BE9F8D0419928CA/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/51EDBCE210C43D463D6290B76BE9F8D0419928CA/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>WILEY</p></availability><date>2008</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs</title><author><persName><forename type="first">Frank</forename><surname>Schwarz</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Daniel</forename><surname>Rothamel</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Monika</forename><surname>Herten</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Daniel</forename><surname>Ferrari</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Martin</forename><surname>Sager</surname></persName><affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</affiliation></author><author><persName><forename type="first">Jürgen</forename><surname>Becker</surname></persName><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation></author></analytic><monogr><title level="j">Clinical Oral Implants Research</title><idno type="pISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><idno type="DOI">10.1111/(ISSN)1600-0501</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2008-07"></date><biblScope unit="volume">19</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="642">642</biblScope><biblScope unit="page" to="652">652</biblScope></imprint></monogr><idno type="istex">51EDBCE210C43D463D6290B76BE9F8D0419928CA</idno><idno type="DOI">10.1111/j.1600-0501.2008.01537.x-i2</idno><idno type="ArticleID">CLR1537</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2008</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Objectives: The aim of the present study was to immunohistochemically evaluate lateral ridge augmentation using a particulated (BOG) or block (BOB) natural bone mineral biocoated with rhGDF‐5 and rhBMP‐2 in dogs. Materials and methods: Three standardized box‐shaped defects were surgically created at the buccal aspect of the alveolar ridge in each quadrant of eight beagle dogs. After 2 months of healing, the chronic‐type defects were randomly allocated in a split‐mouth design to either (i) BOG or (ii) BOB biocoated with (a) rhGDF‐5 or (b) rhBMP‐2, respectively. Uncoated grafts served as controls. After 3 and 8 weeks, dissected blocks were prepared for immunohistochemical [osteocalcin (OC)] and histomorphometrical analysis [e.g. area (mm2) of new bone fill (BF), newly formed mineralized (MT) and non‐mineralized tissue (NMT)]. Results: rhBMP‐2 biocoated BOG revealed significantly highest BF and MT values at 3 (upper and lower jaws – UJ/LJ – compared with BOG) and 8 weeks (UJ – compared with rhGDF‐5). Biocoating of BOB using both rhGDF‐5 and rhBMP‐2 resulted in significantly increased MT values at 8 weeks (UJ/LJ – compared with BOB). In all groups, NMT adjacent to BOG and BOB scaffolds revealed pronounced signs of an OC antigen reactivity. Conclusions: Within the limits of the present study, it was concluded that both rhGDF‐5 and rhBMP‐2 have shown efficacy; however, their bone regenerative effect was markedly influenced by the carrier.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>animal experiments</term></item><item><term>bone substitutes</term></item><item><term>growth factors</term></item><item><term>guided bone regeneration</term></item><item><term>morphometric analysis</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-07">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/51EDBCE210C43D463D6290B76BE9F8D0419928CA/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1600-0501</doi><issn type="print">0905-7161</issn><issn type="electronic">1600-0501</issn><idGroup><id type="product" value="CLR"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="CLINICAL ORAL IMPLANTS RESEARCH">Clinical Oral Implants Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="07007"><doi origin="wiley">10.1111/clr.2008.19.issue-7</doi><numberingGroup><numbering type="journalVolume" number="19">19</numbering><numbering type="journalIssue" number="7">7</numbering></numberingGroup><coverDate startDate="2008-07">July 2008</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="2" status="forIssue"><doi origin="wiley">10.1111/j.1600-0501.2008.01537.x-i2</doi><idGroup><id type="unit" value="CLR1537"></id><id type="supplier" value="1537"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright> © 2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard</copyright><eventGroup><event type="firstOnline" date="2008-05-19"></event><event type="publishedOnlineFinalForm" date="2008-05-19"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:FullText result:FullText" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-12"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="642">642</numbering><numbering type="pageLast" number="652">652</numbering></numberingGroup><correspondenceTo><b>Correspondence to:</b>
<i>PD Dr Frank Schwarz</i>
Department of Oral Surgery
Westdeutsche Kieferklinik
Heinrich Heine University
D‐40225 Düsseldorf
Germany
Tel.: +49 211 8118149
Fax: +49 211 1713542
e‐mail: <email normalForm="Frank.Schwarz@med.uni-duesseldorf.de">Frank.Schwarz@med.uni‐duesseldorf.de</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CLR.CLR1537.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory><b>Date:</b> Accepted 10 October 2007</unparsedEditorialHistory><countGroup><count type="figureTotal" number="10"></count><count type="tableTotal" number="1"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="44"></count><count type="wordTotal" number="7799"></count><count type="linksCrossRef" number="53"></count></countGroup><titleGroup><title type="main">Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs</title><title type="shortAuthors">Schwarz et al.</title><title type="short">Bone regenerative effect of rhGDF‐5 and rhBMP‐2</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Frank</givenNames><familyName>Schwarz</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Daniel</givenNames><familyName>Rothamel</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Monika</givenNames><familyName>Herten</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>Daniel</givenNames><familyName>Ferrari</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>Martin</givenNames><familyName>Sager</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>Jürgen</givenNames><familyName>Becker</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="DE"><unparsedAffiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="DE"><unparsedAffiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">animal experiments</keyword><keyword xml:id="k2">bone substitutes</keyword><keyword xml:id="k3">growth factors</keyword><keyword xml:id="k4">guided bone regeneration</keyword><keyword xml:id="k5">morphometric analysis</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p><b>Objectives: </b> The aim of the present study was to immunohistochemically evaluate lateral ridge augmentation using a particulated (BOG) or block (BOB) natural bone mineral biocoated with rhGDF‐5 and rhBMP‐2 in dogs.</p><p><b>Materials and methods: </b> Three standardized box‐shaped defects were surgically created at the buccal aspect of the alveolar ridge in each quadrant of eight beagle dogs. After 2 months of healing, the chronic‐type defects were randomly allocated in a split‐mouth design to either (i) BOG or (ii) BOB biocoated with (a) rhGDF‐5 or (b) rhBMP‐2, respectively. Uncoated grafts served as controls. After 3 and 8 weeks, dissected blocks were prepared for immunohistochemical [osteocalcin (OC)] and histomorphometrical analysis [e.g. area (mm<sup>2</sup>) of new bone fill (BF), newly formed mineralized (MT) and non‐mineralized tissue (NMT)].</p><p><b>Results: </b> rhBMP‐2 biocoated BOG revealed significantly highest BF and MT values at 3 (upper and lower jaws – UJ/LJ – compared with BOG) and 8 weeks (UJ – compared with rhGDF‐5). Biocoating of BOB using both rhGDF‐5 and rhBMP‐2 resulted in significantly increased MT values at 8 weeks (UJ/LJ – compared with BOB). In all groups, NMT adjacent to BOG and BOB scaffolds revealed pronounced signs of an OC antigen reactivity.</p><p><b>Conclusions: </b> Within the limits of the present study, it was concluded that both rhGDF‐5 and rhBMP‐2 have shown efficacy; however, their bone regenerative effect was markedly influenced by the carrier.</p><!-- To cite this article:
Schwarz F, Rothamel D, Herten M, Ferrari D, Sager M, Becker J. Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF-5 and rhBMP-2: an immunohistochemical study in dogs.
Clin. Oral Impl. Res. 19, 2008; 642–652
10.1111/j.1600-0501.2008.01537.x
</abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs</title></titleInfo><titleInfo type="abbreviated"><title>Bone regenerative effect of rhGDF‐5 and rhBMP‐2</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Lateral ridge augmentation using particulated or block bone substitutes biocoated with rhGDF‐5 and rhBMP‐2: an immunohistochemical study in dogs</title></titleInfo><name type="personal"><namePart type="given">Frank</namePart><namePart type="family">Schwarz</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Daniel</namePart><namePart type="family">Rothamel</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Monika</namePart><namePart type="family">Herten</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Daniel</namePart><namePart type="family">Ferrari</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Martin</namePart><namePart type="family">Sager</namePart><affiliation>Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jürgen</namePart><namePart type="family">Becker</namePart><affiliation>Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2008-07</dateIssued><edition>Date: Accepted 10 October 2007</edition><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">10</extent><extent unit="tables">1</extent><extent unit="references">44</extent><extent unit="words">7799</extent></physicalDescription><abstract lang="en">Objectives: The aim of the present study was to immunohistochemically evaluate lateral ridge augmentation using a particulated (BOG) or block (BOB) natural bone mineral biocoated with rhGDF‐5 and rhBMP‐2 in dogs. Materials and methods: Three standardized box‐shaped defects were surgically created at the buccal aspect of the alveolar ridge in each quadrant of eight beagle dogs. After 2 months of healing, the chronic‐type defects were randomly allocated in a split‐mouth design to either (i) BOG or (ii) BOB biocoated with (a) rhGDF‐5 or (b) rhBMP‐2, respectively. Uncoated grafts served as controls. After 3 and 8 weeks, dissected blocks were prepared for immunohistochemical [osteocalcin (OC)] and histomorphometrical analysis [e.g. area (mm2) of new bone fill (BF), newly formed mineralized (MT) and non‐mineralized tissue (NMT)]. Results: rhBMP‐2 biocoated BOG revealed significantly highest BF and MT values at 3 (upper and lower jaws – UJ/LJ – compared with BOG) and 8 weeks (UJ – compared with rhGDF‐5). Biocoating of BOB using both rhGDF‐5 and rhBMP‐2 resulted in significantly increased MT values at 8 weeks (UJ/LJ – compared with BOB). In all groups, NMT adjacent to BOG and BOB scaffolds revealed pronounced signs of an OC antigen reactivity. Conclusions: Within the limits of the present study, it was concluded that both rhGDF‐5 and rhBMP‐2 have shown efficacy; however, their bone regenerative effect was markedly influenced by the carrier.</abstract><subject lang="en"><genre>Keywords</genre><topic>animal experiments</topic><topic>bone substitutes</topic><topic>growth factors</topic><topic>guided bone regeneration</topic><topic>morphometric analysis</topic></subject><relatedItem type="host"><titleInfo><title>Clinical Oral Implants Research</title></titleInfo><genre type="Journal">journal</genre><identifier type="ISSN">0905-7161</identifier><identifier type="eISSN">1600-0501</identifier><identifier type="DOI">10.1111/(ISSN)1600-0501</identifier><identifier type="PublisherID">CLR</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>19</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>642</start><end>652</end><total>11</total></extent></part></relatedItem><identifier type="istex">51EDBCE210C43D463D6290B76BE9F8D0419928CA</identifier><identifier type="DOI">10.1111/j.1600-0501.2008.01537.x-i2</identifier><identifier type="ArticleID">CLR1537</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2008 The Authors. 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