Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles
Identifieur interne : 000432 ( Main/Corpus ); précédent : 000431; suivant : 000433Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles
Auteurs : Athanasios Zovoilis ; Jessica Nolte ; Nadja Drusenheimer ; Ulrich Zechner ; Hiroki Hada ; Kaomei Guan ; Gerd Hasenfuss ; Karim Nayernia ; Wolfgang EngelSource :
- Molecular Human Reproduction [ 1360-9947 ] ; 2008-09.
Abstract
Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of in vitro differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues.
Url:
DOI: 10.1093/molehr/gan044
Links to Exploration step
ISTEX:72795F8315C90A38A44D4CBFC08D3270BE060C56Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</title><author><name sortKey="Zovoilis, Athanasios" sort="Zovoilis, Athanasios" uniqKey="Zovoilis A" first="Athanasios" last="Zovoilis">Athanasios Zovoilis</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: azovoil@gwdg.de</mods:affiliation></affiliation></author><author><name sortKey="Nolte, Jessica" sort="Nolte, Jessica" uniqKey="Nolte J" first="Jessica" last="Nolte">Jessica Nolte</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author><author><name sortKey="Drusenheimer, Nadja" sort="Drusenheimer, Nadja" uniqKey="Drusenheimer N" first="Nadja" last="Drusenheimer">Nadja Drusenheimer</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author><author><name sortKey="Zechner, Ulrich" sort="Zechner, Ulrich" uniqKey="Zechner U" first="Ulrich" last="Zechner">Ulrich Zechner</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Mainz, Mainz 55131, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hada, Hiroki" sort="Hada, Hiroki" uniqKey="Hada H" first="Hiroki" last="Hada">Hiroki Hada</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author><author><name sortKey="Guan, Kaomei" sort="Guan, Kaomei" uniqKey="Guan K" first="Kaomei" last="Guan">Kaomei Guan</name><affiliation><mods:affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hasenfuss, Gerd" sort="Hasenfuss, Gerd" uniqKey="Hasenfuss G" first="Gerd" last="Hasenfuss">Gerd Hasenfuss</name><affiliation><mods:affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</mods:affiliation></affiliation></author><author><name sortKey="Nayernia, Karim" sort="Nayernia, Karim" uniqKey="Nayernia K" first="Karim" last="Nayernia">Karim Nayernia</name><affiliation><mods:affiliation>Institute of Human Genetics, International Centre for Life, University of Newcastle, Newcastle upon Tyne NE1 3BZ, UK</mods:affiliation></affiliation></author><author><name sortKey="Engel, Wolfgang" sort="Engel, Wolfgang" uniqKey="Engel W" first="Wolfgang" last="Engel">Wolfgang Engel</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:72795F8315C90A38A44D4CBFC08D3270BE060C56</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1093/molehr/gan044</idno><idno type="url">https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000432</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</title><author><name sortKey="Zovoilis, Athanasios" sort="Zovoilis, Athanasios" uniqKey="Zovoilis A" first="Athanasios" last="Zovoilis">Athanasios Zovoilis</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: azovoil@gwdg.de</mods:affiliation></affiliation></author><author><name sortKey="Nolte, Jessica" sort="Nolte, Jessica" uniqKey="Nolte J" first="Jessica" last="Nolte">Jessica Nolte</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author><author><name sortKey="Drusenheimer, Nadja" sort="Drusenheimer, Nadja" uniqKey="Drusenheimer N" first="Nadja" last="Drusenheimer">Nadja Drusenheimer</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author><author><name sortKey="Zechner, Ulrich" sort="Zechner, Ulrich" uniqKey="Zechner U" first="Ulrich" last="Zechner">Ulrich Zechner</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Mainz, Mainz 55131, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hada, Hiroki" sort="Hada, Hiroki" uniqKey="Hada H" first="Hiroki" last="Hada">Hiroki Hada</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author><author><name sortKey="Guan, Kaomei" sort="Guan, Kaomei" uniqKey="Guan K" first="Kaomei" last="Guan">Kaomei Guan</name><affiliation><mods:affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hasenfuss, Gerd" sort="Hasenfuss, Gerd" uniqKey="Hasenfuss G" first="Gerd" last="Hasenfuss">Gerd Hasenfuss</name><affiliation><mods:affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</mods:affiliation></affiliation></author><author><name sortKey="Nayernia, Karim" sort="Nayernia, Karim" uniqKey="Nayernia K" first="Karim" last="Nayernia">Karim Nayernia</name><affiliation><mods:affiliation>Institute of Human Genetics, International Centre for Life, University of Newcastle, Newcastle upon Tyne NE1 3BZ, UK</mods:affiliation></affiliation></author><author><name sortKey="Engel, Wolfgang" sort="Engel, Wolfgang" uniqKey="Engel W" first="Wolfgang" last="Engel">Wolfgang Engel</name><affiliation><mods:affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Human Reproduction</title><idno type="ISSN">1360-9947</idno><idno type="eISSN">1460-2407</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2008-09">2008-09</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="521">521</biblScope><biblScope unit="page" to="529">529</biblScope></imprint><idno type="ISSN">1360-9947</idno></series><idno type="istex">72795F8315C90A38A44D4CBFC08D3270BE060C56</idno><idno type="DOI">10.1093/molehr/gan044</idno><idno type="ArticleID">gan044</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1360-9947</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of in vitro differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Athanasios Zovoilis</name><affiliations><json:string>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</json:string><json:string>E-mail: azovoil@gwdg.de</json:string></affiliations></json:item><json:item><name>Jessica Nolte</name><affiliations><json:string>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</json:string></affiliations></json:item><json:item><name>Nadja Drusenheimer</name><affiliations><json:string>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</json:string></affiliations></json:item><json:item><name>Ulrich Zechner</name><affiliations><json:string>Institute of Human Genetics, University of Mainz, Mainz 55131, Germany</json:string></affiliations></json:item><json:item><name>Hiroki Hada</name><affiliations><json:string>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</json:string></affiliations></json:item><json:item><name>Kaomei Guan</name><affiliations><json:string>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</json:string></affiliations></json:item><json:item><name>Gerd Hasenfuss</name><affiliations><json:string>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</json:string></affiliations></json:item><json:item><name>Karim Nayernia</name><affiliations><json:string>Institute of Human Genetics, International Centre for Life, University of Newcastle, Newcastle upon Tyne NE1 3BZ, UK</json:string></affiliations></json:item><json:item><name>Wolfgang Engel</name><affiliations><json:string>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Articles</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>multipotent adult germline stem cells</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>embryonic stem cells</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>microRNAs</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Oct4</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pluripotency markers</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of in vitro differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues.</abstract><qualityIndicators><score>7.772</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612.283 x 790.866 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>6</keywordCount><abstractCharCount>1570</abstractCharCount><pdfWordCount>6413</pdfWordCount><pdfCharCount>38429</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>231</abstractWordCount></qualityIndicators><title>Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</title><genre><json:string>research-article</json:string></genre><host><volume>14</volume><pages><last>529</last><first>521</first></pages><issn><json:string>1360-9947</json:string></issn><issue>9</issue><genre></genre><language><json:string>unknown</json:string></language><eissn><json:string>1460-2407</json:string></eissn><title>Molecular Human Reproduction</title></host><categories><wos><json:string>DEVELOPMENTAL BIOLOGY</json:string><json:string>REPRODUCTIVE BIOLOGY</json:string></wos></categories><publicationDate>2008</publicationDate><copyrightDate>2008</copyrightDate><doi><json:string>10.1093/molehr/gan044</json:string></doi><id>72795F8315C90A38A44D4CBFC08D3270BE060C56</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability><p>OUP</p></availability><date>2008-08-12</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</title><author><persName><forename type="first">Athanasios</forename><surname>Zovoilis</surname></persName><email>azovoil@gwdg.de</email><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation></author><author><persName><forename type="first">Jessica</forename><surname>Nolte</surname></persName><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation></author><author><persName><forename type="first">Nadja</forename><surname>Drusenheimer</surname></persName><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation></author><author><persName><forename type="first">Ulrich</forename><surname>Zechner</surname></persName><affiliation>Institute of Human Genetics, University of Mainz, Mainz 55131, Germany</affiliation></author><author><persName><forename type="first">Hiroki</forename><surname>Hada</surname></persName><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation></author><author><persName><forename type="first">Kaomei</forename><surname>Guan</surname></persName><affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</affiliation></author><author><persName><forename type="first">Gerd</forename><surname>Hasenfuss</surname></persName><affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</affiliation></author><author><persName><forename type="first">Karim</forename><surname>Nayernia</surname></persName><affiliation>Institute of Human Genetics, International Centre for Life, University of Newcastle, Newcastle upon Tyne NE1 3BZ, UK</affiliation></author><author><persName><forename type="first">Wolfgang</forename><surname>Engel</surname></persName><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation></author></analytic><monogr><title level="j">Molecular Human Reproduction</title><idno type="JournalID">molehr</idno><idno type="pISSN">1360-9947</idno><idno type="eISSN">1460-2407</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2008-09"></date><biblScope unit="volume">14</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="521">521</biblScope><biblScope unit="page" to="529">529</biblScope></imprint></monogr><idno type="istex">72795F8315C90A38A44D4CBFC08D3270BE060C56</idno><idno type="DOI">10.1093/molehr/gan044</idno><idno type="ArticleID">gan044</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2008-08-12</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of in vitro differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues.</p></abstract><textClass><keywords scheme="keyword"><list><head>heading</head><item><term>Articles</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>multipotent adult germline stem cells</term></item><item><term>embryonic stem cells</term></item><item><term>microRNAs</term></item><item><term>Oct4</term></item><item><term>pluripotency markers</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-08-12">Created</change><change when="2008-09">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="publisher-id">molehr</journal-id><journal-id journal-id-type="hwp">molehr</journal-id><journal-title>Molecular Human Reproduction</journal-title><issn pub-type="ppub">1360-9947</issn><issn pub-type="epub">1460-2407</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/molehr/gan044</article-id><article-id pub-id-type="publisher-id">gan044</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><series-title>editor's choice</series-title></article-categories><title-group><article-title>Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zovoilis</surname><given-names>Athanasios</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="corresp" rid="cor1">5</xref></contrib><contrib contrib-type="author"><name><surname>Nolte</surname><given-names>Jessica</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Drusenheimer</surname><given-names>Nadja</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zechner</surname><given-names>Ulrich</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Hada</surname><given-names>Hiroki</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Guan</surname><given-names>Kaomei</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Hasenfuss</surname><given-names>Gerd</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Nayernia</surname><given-names>Karim</given-names></name><xref ref-type="aff" rid="af4">4</xref></contrib><contrib contrib-type="author"><name><surname>Engel</surname><given-names>Wolfgang</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib></contrib-group><aff id="af1"><label>1</label><addr-line>Institute of Human Genetics</addr-line>, <institution>University of Goettingen</institution>, <addr-line>Heinrich-Dueker- Weg 12, Goettingen D-37073</addr-line>, <country>Germany</country></aff><aff id="af2"><label>2</label><addr-line>Institute of Human Genetics</addr-line>, <institution>University of Mainz</institution>, <addr-line>Mainz 55131</addr-line>, <country>Germany</country></aff><aff id="af3"><label>3</label><addr-line>Department of Cardiology and Pneumology</addr-line>, <institution>University of Goettingen</institution>, <addr-line>Goettingen 37075</addr-line>, <country>Germany</country></aff><aff id="af4"><label>4</label><addr-line>Institute of Human Genetics, International Centre for Life</addr-line>, <institution>University of Newcastle</institution>, <addr-line>Newcastle upon Tyne NE1 3BZ</addr-line>, <country>UK</country></aff><author-notes><corresp id="cor1"><label>5</label>Correspondence address. Tel: <phone>+49-551-397589</phone>; Fax: <fax>+49-551-399303</fax>; E-mail: <email>azovoil@gwdg.de</email></corresp></author-notes><pub-date pub-type="ppub"><month>9</month><year>2008</year></pub-date><pub-date pub-type="epub"><day>12</day><month>8</month><year>2008</year></pub-date><volume>14</volume><issue>9</issue><fpage>521</fpage><lpage>529</lpage><history><date date-type="received"><day>29</day><month>3</month><year>2008</year></date><date date-type="rev-recd"><day>24</day><month>7</month><year>2008</year></date><date date-type="accepted"><day>6</day><month>8</month><year>2008</year></date></history><copyright-statement>© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org</copyright-statement><copyright-year>2008</copyright-year><abstract><p>Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of <italic>in vitro</italic> differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues.</p></abstract><kwd-group><title>Keywords</title><kwd>multipotent adult germline stem cells</kwd><kwd>embryonic stem cells</kwd><kwd>microRNAs</kwd><kwd>Oct4</kwd><kwd>pluripotency markers</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><partName>editor's choice</partName><title>Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><partName>editor's choice</partName><title>Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles</title></titleInfo><name type="personal"><namePart type="given">Athanasios</namePart><namePart type="family">Zovoilis</namePart><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation><affiliation>E-mail: azovoil@gwdg.de</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jessica</namePart><namePart type="family">Nolte</namePart><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nadja</namePart><namePart type="family">Drusenheimer</namePart><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ulrich</namePart><namePart type="family">Zechner</namePart><affiliation>Institute of Human Genetics, University of Mainz, Mainz 55131, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hiroki</namePart><namePart type="family">Hada</namePart><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kaomei</namePart><namePart type="family">Guan</namePart><affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gerd</namePart><namePart type="family">Hasenfuss</namePart><affiliation>Department of Cardiology and Pneumology, University of Goettingen, Goettingen 37075, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Karim</namePart><namePart type="family">Nayernia</namePart><affiliation>Institute of Human Genetics, International Centre for Life, University of Newcastle, Newcastle upon Tyne NE1 3BZ, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wolfgang</namePart><namePart type="family">Engel</namePart><affiliation>Institute of Human Genetics, University of Goettingen, Heinrich-Dueker- Weg 12, Goettingen D-37073, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article">research-article</genre><subject><genre>heading</genre><topic>Articles</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2008-09</dateIssued><dateCreated encoding="w3cdtf">2008-08-12</dateCreated><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell types. Despite the differences regarding time and extent of differentiation observed between ESCs and maGSCs, expression profiles of both miRNA families showed similarities between these two cell types, suggesting similar underlying mechanisms in maintenance of pluripotency and differentiation. Our results indicate that the 290-miRNA family is connected with Oct-4 and maintenance of the pluripotent state. In contrast, members of the 302-miRNA family are induced during first stages of in vitro differentiation in all cell types tested. Therefore, detection of miRNAs of miR-302 family in pluripotent cells can be attributed to the proportion of spontaneously differentiating cells in cultures of pluripotent cells. These results are consistent with ESC-like nature of maGSCs and their potential as an alternative source of pluripotent cells from non-embryonic tissues.</abstract><subject><genre>Keywords</genre><topic>multipotent adult germline stem cells</topic><topic>embryonic stem cells</topic><topic>microRNAs</topic><topic>Oct4</topic><topic>pluripotency markers</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Human Reproduction</title></titleInfo><identifier type="ISSN">1360-9947</identifier><identifier type="eISSN">1460-2407</identifier><identifier type="JournalID">molehr</identifier><identifier type="JournalID-hwp">molehr</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>14</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>521</start><end>529</end></extent></part></relatedItem><identifier type="istex">72795F8315C90A38A44D4CBFC08D3270BE060C56</identifier><identifier type="DOI">10.1093/molehr/gan044</identifier><identifier type="ArticleID">gan044</identifier><accessCondition type="use and reproduction" contentType="Copyright">The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissionsoxfordjournals.org</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/tiff</mimetype><extension>tiff</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/covers/tiff</uri></json:item><json:item><original>true</original><mimetype>text/html</mimetype><extension>html</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/covers/html</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/annexes/gif</uri></json:item><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/annexes/pdf</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/72795F8315C90A38A44D4CBFC08D3270BE060C56/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">DEVELOPMENTAL BIOLOGY</classCode><classCode scheme="WOS">REPRODUCTIVE BIOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/SchutzV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000432 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000432 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= SchutzV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= ISTEX:72795F8315C90A38A44D4CBFC08D3270BE060C56
|texte= Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles
}}
| This area was generated with Dilib version V0.6.38. |  |